Abstract:
:Many, if not all, mRNAs in T.brucei start with the same sequence of 35 nucleotides, separately encoded in clustered so-called mini-exon repeats. From these mini-exon repeats a 141-nt precursor RNA with the 35-nt sequence at its 5' end is transcribed. Indirect evidence suggests that this RNA is linked in a second step to pre-mRNA transcripts. We have studied the sensitivity of RNA synthesis to alpha-amanitin in isolated trypanosome nuclei. Transcription of several protein coding genes is almost completely inhibited by a concentration of 5 micrograms alpha-amanitin per ml, whereas strong inhibition of mini-exon transcription is achieved with 200 micrograms alpha-amanitin per ml. In contrast, transcription of genes for variant surface glycoproteins (VSGs) is not inhibited by 1000 micrograms alpha-amanitin per ml, as is transcription of the genes for the major rRNAs. Since the mature VSG mRNAs start with the 35-nt sequence, our results provide additional evidence that the 35-nt sequence and the main part of VSG mRNA are produced from independent transcription units, these are transcribed by (partly) different RNA polymerases.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Kooter JM,Borst Pdoi
10.1093/nar/12.24.9457subject
Has Abstractpub_date
1984-12-21 00:00:00pages
9457-72issue
24eissn
0305-1048issn
1362-4962journal_volume
12pub_type
杂志文章abstract::The SeqA protein binds hemi-methylated GATC sites and forms structures that sequester newly replicated origins and trail the replication forks. Cells that lack SeqA display signs of replication fork disintegration. The broken forks could arise because of over-initiation (the launching of too many forks) or lack of dyn...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx263
更新日期:2017-06-20 00:00:00
abstract::This paper presents TurboFold II, an extension of the TurboFold algorithm for predicting secondary structures for multiple RNA homologs. TurboFold II augments the structure prediction capabilities of TurboFold by additionally providing multiple sequence alignments. Probabilities for alignment of nucleotide positions b...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx815
更新日期:2017-11-16 00:00:00
abstract::Chromatin footprinting in Drosophila tissue culture cells has detected the binding of a non-histone protein at +8 of the distal Adh RNA start site, on a 10-bp direct repeat motif abutting a nucleosome positioned over the inactive Adh distal promoter. Alternatively the active promoter is bound by a transcription initia...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.17.4481
更新日期:1992-09-11 00:00:00
abstract::The use of the ammonia deprotection step to introduce (15)N labels at specific exocyclic amino positions of adenine, cytosine, guanine or 2-aminopurine of oligodeoxynucleotides is described. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.15.2982
更新日期:1994-08-11 00:00:00
abstract::The linking number of DNA in minichromosomes increases by 2 turns during SV40 assembly. Changes in temperature also influence the average linking number of the total intracellular forms of SV40 DNA. When the isolated minichromosomes assembled in vivo are incubated with topoisomerase I at 33 degrees C in vitro, the lin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.9.3703
更新日期:1987-05-11 00:00:00
abstract::Fully protected diastereoisomers of deoxyguanylyl (3' leads to 5') deoxyadenosine stereospecifically labelled on phosphorus with oxygen-18 have been synthesized by oxidation of phosphite triester intermediates in the presence of 18O-labelled water. The diastereoisomers have been chromatographically separated and their...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.20.7087
更新日期:1983-10-25 00:00:00
abstract::As protein-protein interactions are crucial in most biological processes, it is valuable to understand how and where protein pairs interact. We developed a web server HOMCOS (Homology Modeling of Complex Structure, http://biunit.naist.jp/homcos) to predict interacting protein pairs and interacting sites by homology mo...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn218
更新日期:2008-07-01 00:00:00
abstract::Publicly available database of co-expressed gene sets would be a valuable tool for a wide variety of experimental designs, including targeting of genes for functional identification or for regulatory investigation. Here, we report the construction of an Arabidopsis thaliana trans-factor and cis-element prediction data...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl783
更新日期:2007-01-01 00:00:00
abstract::We report the use of an engineered ribozyme to produce a circular human exon in vitro. Specifically, we have designed a derivative of a yeast self-splicing group II intron that is able to catalyze the formation of a circular exon encoding the first kringle domain (K1) of the human tissue plasminogen activator protein....
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.24.5085
更新日期:1997-12-15 00:00:00
abstract::It is well established that gene regulation can be achieved through activator and repressor proteins that bind to DNA and switch particular genes on or off, and that complex metabolic networks determine the levels of transcription of a given gene at a given time. Using three complementary computational techniques to s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw815
更新日期:2016-11-02 00:00:00
abstract::The nucleotide sequence of a region located on the adenovirus type 5 genome between coordinates 59.9 and 62.5 has been determined. This region comprises the 3'-termini of mRNAs coding for the hexon and the DNA binding protein. The sequence reveals the presence of an open reading frame in r-strand transcripts downstrea...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.24.6033
更新日期:1980-12-20 00:00:00
abstract::Comparative genome hybridization (CGH) to DNA microarrays (array CGH) is a technique capable of detecting deletions and duplications in genomes at high resolution. However, array CGH studies of the human genome noting false negative and false positive results using large insert clones as probes have raised important c...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki643
更新日期:2005-06-16 00:00:00
abstract::The oncogenic transformation of normal cells into malignant, rapidly proliferating cells requires major alterations in cell physiology. For example, the transformed cells remodel their metabolic processes to supply the additional demand for cellular building blocks. We have recently demonstrated essential metabolic pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1337
更新日期:2016-01-04 00:00:00
abstract::The essential elements of the sea urchin L. variegatus U1 snRNA promoter were mapped by microinjection of a U1 maxigene into sea urchin zygotes. Two elements are required for expression: a distal sequence element (DSE) located between -318 and -300 and a proximal sequence element (PSE) centered at -55. Removal or alte...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.14.3743
更新日期:1992-07-25 00:00:00
abstract::We propose a novel mechanism of gene regulation in Mycobacterium tuberculosis where the protein Rv1222 inhibits transcription by anchoring RNA polymerase (RNAP) onto DNA. In contrast to our existing knowledge that transcriptional repressors function either by binding to DNA at specific sequences or by binding to RNAP,...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv516
更新日期:2015-07-13 00:00:00
abstract::In order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. A 16-mer oligonucleotide (ODN) was derivatized at the 5' prime through a 32P phosphate spacer with cholesterol, yielding a 32P-labeled amphiphatic cho...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.17.4695
更新日期:1991-09-11 00:00:00
abstract::Natural oligopeptides may regulate nearly all vital processes. To date, the chemical structures of nearly 6000 oligopeptides have been identified from >1000 organisms representing all the biological kingdoms. We have compiled the known physical, chemical and biological properties of these oligopeptides--whether synthe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkj008
更新日期:2006-01-01 00:00:00
abstract::We have studied the effect of temperature on transcription initiation in vitro at two related promoters ga/Pcon and ga/P1, which have the same nucleotide sequence around the -10 region and transcription start site, but differ in upstream sequences. One of the promoters, ga/Pcon, carries the consensus -35 hexamer, 5'TT...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.22.6113
更新日期:1991-11-25 00:00:00
abstract::One of the most intensively studied chromatin binding factors is HP1α. HP1α is associated with silenced, heterochromatic regions of the genome and binds to H3K9me3. While H3K9me3 is necessary for HP1α recruitment to heterochromatin, it is becoming apparent that it is not sufficient suggesting that additional factors a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky632
更新日期:2018-10-12 00:00:00
abstract::We determined the structure around the gap region between 28S alpha and 28S beta rRNA of Bombyx mori, a lepidopteran insect, to know the introduction mechanism of the hidden break, an interruption of the phosphodiester bond specific to the 28S rRNA of protostomes. Sequence analysis and S1 nuclease mapping suggested th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.16.6393
更新日期:1986-08-26 00:00:00
abstract::Telomere G-quadruplex is emerging as a promising anti-cancer target due to its inhibition to telomerase, an enzyme expressed in more than 85% tumors. Telomerase-mediated telomere extension and some other reactions require a free 3' telomere end in single-stranded form. G-quadruplex formation near the 3' end of telomer...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr164
更新日期:2011-08-01 00:00:00
abstract::Transcriptionally non-co-linear (NCL) transcripts can originate from trans-splicing (trans-spliced RNA; 'tsRNA') or cis-backsplicing (circular RNA; 'circRNA'). While numerous circRNAs have been detected in various species, tsRNAs remain largely uninvestigated. Here, we utilize integrative transcriptome sequencing of p...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky032
更新日期:2018-04-20 00:00:00
abstract::TOP mRNAs are vertebrate transcripts which contain a 5'terminal oligopyrimidine tract (5'TOP), encode for ribosomal proteins and elongation factors 1alpha and 2, and are candidates for growth-dependent translational control mediated through their 5'TOP. In the present study we show that elongation factor 2 (EF2) mRNA ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.5.995
更新日期:1997-03-01 00:00:00
abstract::Human somatic cells have essentially no telomerase activity. Telomerase is linked to tumor genesis and is a valuable marker for malignant growth. Extreme paucity of the enzyme neccessitated development of a PCR-based assay, 'telomeric repeat amplification protocol' (TRAP). Unfortunately, this method is not without dif...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.4.919
更新日期:1997-02-15 00:00:00
abstract::Transgenic mice have proven to be an effective expression system for studying developmental control of the human fetal and adult beta-globin genes. In the current work we are interested in developing the transgenic mouse system for the study of the human embryonic beta-globin gene, epsilon. An epsilon-globin gene cons...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.18.5465
更新日期:1990-09-25 00:00:00
abstract::The ability of Deinococcus radiodurans to recover from extensive DNA damage is due in part to its ability to efficiently repair its genome, even following severe fragmentation by hundreds of double-strand breaks. The single-strand annealing pathway plays an important role early during the recovery process, making use ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt759
更新日期:2013-11-01 00:00:00
abstract::Protein-RNA interactions play essential roles in a number of regulatory mechanisms for gene expression such as RNA splicing, transport, translation and post-transcriptional control. As the number of available protein-RNA complex 3D structures has increased, it is now possible to statistically examine protein-RNA inter...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl819
更新日期:2006-01-01 00:00:00
abstract::Corynebacterium diphtheriae is a Gram-positive, non-spore forming, non-motile, pleomorphic rod belonging to the genus Corynebacterium and the actinomycete group of organisms. The organism produces a potent bacteriophage-encoded protein exotoxin, diphtheria toxin (DT), which causes the symptoms of diphtheria. This pote...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg874
更新日期:2003-11-15 00:00:00
abstract::DNase I and MPE.Fe (II) footprinting both employ partial cleavage of ligand-protected DNA restriction fragments and Maxam-Gilbert sequencing gel methods of analysis. One method utilizes the enzyme, DNase I, as the DNA cleaving agent while the other employs the synthetic molecule, methidium-propyl-EDTA (MPE). For actin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.16.5555
更新日期:1983-08-25 00:00:00
abstract::With recent advancements in techniques for cellular data acquisition, information on cellular processes has been increasing at a dramatic rate. Visualization is critical to analyzing and interpreting complex information; representing cellular processes or pathways is no exception. VISIBIOweb is a free, open-source, we...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq352
更新日期:2010-07-01 00:00:00