Abstract:
:We have studied the effect of temperature on transcription initiation in vitro at two related promoters ga/Pcon and ga/P1, which have the same nucleotide sequence around the -10 region and transcription start site, but differ in upstream sequences. One of the promoters, ga/Pcon, carries the consensus -35 hexamer, 5'TTGACA 3', whilst ga/P1 contains a block of 'distortable' upstream sequences that allow promoter function in the absence of a -35 region consensus sequence. RNA polymerase can form complexes with both promoters at a range of temperatures. However, the thermal energy requirement for open complex formation differs: open complexes can form at ga/P1 at low temperatures, whereas ga/Pcon requires higher temperatures. The thermal energy requirement for transcription from preformed open complexes is the same for both promoters.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Grimes E,Busby S,Minchin Sdoi
10.1093/nar/19.22.6113subject
Has Abstractpub_date
1991-11-25 00:00:00pages
6113-8issue
22eissn
0305-1048issn
1362-4962journal_volume
19pub_type
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