Abstract:
:The gene-external part of the human 7S L promoter was analyzed by transcription in vitro and in vivo. Compared to the wild type promoter (-178), a -66 5'deletion mutant revealed full activity in vitro but was inefficiently transcribed in vivo. Further deletion to -37 reduced template activity to 50% in vitro and to basal level expression in vivo (below 5%). A DNase I footprint observed around position -50 protected an ATF-like binding site ('TGACGT'). With respect to 7S L transcription regulation, the functionality of this ATF-like binding site was confirmed in competition experiments and by mutation analysis. Furthermore, S100 extracts of cells pretreated with forskolin in vivo to induce the cAMP system, revealed significantly increased transcription of 7S L RNA in vitro, with no effect on a 7S K RNA gene, lacking such an ATF binding site. Thus, the 7S L RNA gene too is controlled by a regulatory element originally defined in class II promoters and represents another rare example where a specific type of transcription regulation in vivo can be mimicked with cell-free extracts in vitro.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bredow S,Sürig D,Müller J,Kleinert H,Benecke BJdoi
10.1093/nar/18.23.6779subject
Has Abstractpub_date
1990-12-11 00:00:00pages
6779-84issue
23eissn
0305-1048issn
1362-4962journal_volume
18pub_type
杂志文章abstract::The proposition that the 2-amino group of guanine plays a critical role in determining how antibiotics recognise their binding sites in DNA has been tested by relocating it, using tyrT DNA derivative molecules substituted with inosine plus 2,6-diaminopurine (DAP). Irrespective of their mode of interaction with DNA, su...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.6.885
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:2016-06-02 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1992-03-25 00:00:00
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abstract::The gene encoding myelin basic protein (MBP) contains multiple activator sequences spanning upstream of its transcriptional initiation site which differentially promote transcription in glial cells. The proximal activator sequence, designated MB1, activates transcription in a glial cell type specific manner. This sequ...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1988-07-25 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-02-08 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-01-31 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
abstract::Sexual differentiation in Drosophila is regulated through alternative splicing of doublesex. Female-specific splicing is activated through the activity of splicing enhancer complexes assembled on multiple repeat elements. Each of these repeats serves as a binding platform for the cooperative assembly of a heterotrimer...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
abstract::In the battle against viruses, antiviral peptides (AVPs) had demonstrated the immense potential. Presently, more than 15 peptide-based drugs are in various stages of clinical trials. Emerging and re-emerging viruses further emphasize the efforts to accelerate antiviral drug discovery efforts. Despite, huge importance ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks450
更新日期:2012-07-01 00:00:00
abstract::Rad51 protein promotes homologous recombination in eukaryotes. Recombination activities are activated by Rad51 filament assembly on ssDNA. Previous studies of yeast Rad51 showed that His352 occupies an important position at the filament interface, where it could relay signals between subunits and active sites. To inve...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq209
更新日期:2010-08-01 00:00:00
abstract::Over millions of years the structure and complexity of the transcriptional regulatory network (TRN) in bacteria has changed, reorganized and enabled them to adapt to almost every environmental niche on earth. In order to understand the plasticity of TRNs in bacteria, we studied the conservation of currently known TRNs...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl423
更新日期:2006-07-13 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky486
更新日期:2018-09-19 00:00:00
abstract::A list of DNA sequences cloned from the human genome is presented. Intended as a guide to clone availability, this list includes published reports of cDNA, genomic and synthetic clones comprising gene and pseudogene sequences, uncharacterised DNA segments and repetitive DNA elements. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.suppl.r403
更新日期:1988-01-01 00:00:00
abstract::Quantitative real-time PCR (qPCR) is the method of choice for specific and sensitive quantification of nucleic acids. However, data validation is still a major issue, partially due to the complex effect of PCR inhibition on the results. If undetected PCR inhibition may severely impair the accuracy and sensitivity of r...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
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更新日期:2012-02-01 00:00:00
abstract::The far upstream region of herpes simplex virus (HSV) immediate early (IE) gene 3 has previously been shown to increase gene expression in an enhancer-like manner, and to contain sequences which respond to stimulation of transcription by a virion polypeptide, Vmw65. To analyse the specific DNA sequences which mediate ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.2.929
更新日期:1986-01-24 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.5.1111
更新日期:1981-03-11 00:00:00
abstract::The HpaR-mediated regulation of the hpa-meta operon (Pg promoter) of the 4-hydroxyphenylacetic acid catabolic pathway of Escherichia coli has been studied. The HpaR regulator was purified to homogeneity showing that it is able to bind selectively to 4-hydroxyphenylacetic, 3-hydroxyphenylacetic and 3,4-dihydroxyphenyla...
journal_title:Nucleic acids research
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更新日期:2003-11-15 00:00:00
abstract::Denaturing gradient gel electrophoresis (DGGE) is based upon the different melting behaviors of DNA molecules in a chemical denaturant gradient according to their sequences. This technique has recently become a widespread tool to detect mutations. The introduction of a GC-clamp enables the detection of most single bas...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.12.3331
更新日期:1991-06-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt030
更新日期:2013-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-12-25 00:00:00
abstract::Codon usage data has been compiled for 110 yeast genes. Cluster analysis on relative synonymous codon usage revealed two distinct groups of genes. One group corresponds to highly expressed genes, and has much more extreme synonymous codon preference. The pattern of codon usage observed is consistent with that expected...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1986-07-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2020-07-27 00:00:00