Elongation complexes of Thermus thermophilus RNA polymerase that possess distinct translocation conformations.

abstract：:The E.coli ada gene protein coding region has been ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase gene promoter region to produce pADH06C. The yeast strains SX46A, 7799-4B and VV-6 are deficient in endogenous O6-alkylguanine-DNA-alkyltransferase and...

journal_title：Nucleic acids research

authors： Brozmanova J,Kleibl K,Vlckova V,Skorvaga M,Cernakova L,Margison GP

更新日期：1990-01-25 00:00:00

abstract：:The transcriptional activity of a human transposon-like family of repeats, called the THE-1 family, has been studied in cell culture and in human tissue. Both strands of THE-1 are present in several discrete length poly A plus RNAs. Primer extension studies and the structures of cDNA clones show that these THE-1 trans...

journal_title：Nucleic acids research

authors： Paulson KE,Matera AG,Deka N,Schmid CW

更新日期：1987-07-10 00:00:00

abstract：:iSARST is a web server for efficient protein structural similarity searches. It is a multi-processor, batch-processing and integrated implementation of several structural comparison tools and two database searching methods: SARST for common structural homologs and CPSARST for homologs with circular permutations. iSARS...

journal_title：Nucleic acids research

authors： Lo WC,Lee CY,Lee CC,Lyu PC

更新日期：2009-07-01 00:00:00

abstract：:Rapid and transient activation of both c-fos transcription and mRNA accumulation occurs when resting CCL39 hamster fibroblasts are serum-stimulated to grow. By using several combinations of serum and cycloheximide, a protein synthesis inhibitor, we showed that: i) addition of cycloheximide to resting cell elicits an i...

journal_title：Nucleic acids research

authors： Fort P,Rech J,Vie A,Piechaczyk M,Bonnieu A,Jeanteur P,Blanchard JM

更新日期：1987-07-24 00:00:00

abstract：:The goal of the present study is to identify genes that respond to iron availability. Suppression subtraction hybridization (SSH) was used to generate cDNA libraries from iron loaded and control human astrocytoma cells (SW1088). The cDNA libraries were screened with antisense cDNA probes obtained from mRNA isolated fr...

journal_title：Nucleic acids research

authors： Ye Z,Connor JR

更新日期：2000-04-15 00:00:00

abstract：:Specific guanine-rich regions in human genome can form higher-order DNA structures called G-quadruplexes, which regulate many relevant biological processes. For instance, the formation of G-quadruplex at telomeres can alter cellular functions, inducing apoptosis. Thus, developing small molecules that are able to bind ...

journal_title：Nucleic acids research

authors： Di Leva FS,Novellino E,Cavalli A,Parrinello M,Limongelli V

更新日期：2014-05-01 00:00:00

abstract：:Acetyl-CoA Carboxylase catalyzes the first committed step in fatty acid synthesis. Escherichia coli acetyl-CoA carboxylase is composed of biotin carboxylase, carboxyltransferase and biotin carboxyl carrier protein functions. The accA and accD genes that code for the alpha- and beta-subunits, respectively, are not in a...

journal_title：Nucleic acids research

authors： Meades G Jr,Benson BK,Grove A,Waldrop GL

更新日期：2010-03-01 00:00:00

abstract：:The 0.048502 megabase (Mb), primarily double-stranded DNA of bacteriophage lambda has single-stranded, complementary termini (cohesive ends) that undergo either spontaneous intramolecular joining to form open circular DNA or spontaneous intermolecular joining to form linear, end-to-end oligomeric DNAs (concatemers); c...

journal_title：Nucleic acids research

authors： Louie D,Serwer P

更新日期：1991-06-11 00:00:00

abstract：:Investigation of biological processes using selective chemical interventions is generally applied in biomedical research and drug discovery. Many studies of this kind make use of gene expression experiments to explore cellular responses to chemical interventions. Recently, some research groups constructed libraries of...

journal_title：Nucleic acids research

authors： Li Y,Hao P,Zheng S,Tu K,Fan H,Zhu R,Ding G,Dong C,Wang C,Li X,Thiesen HJ,Chen YE,Jiang H,Liu L,Li Y

更新日期：2008-11-01 00:00:00

abstract：:The deoxyoctanucleotide 5'd (AAGGAGGT) which is complementary to the 3' terminus of 16S RNA has been used as a probe to measure the potential of this rRNA region to engage in intermolecular basepairing. The site specific binding of the octanucleotide is shown by labeling 16S RNA in situ at its 3' end with [32P]pCp and...

journal_title：Nucleic acids research

authors： Backendorf C,Ravensbergen CJ,Van der Plas J,van Boom JH,Veeneman G,Van Duin J

更新日期：1981-03-25 00:00:00

abstract：:The E. coli protein StpA has RNA annealing and strand displacement activities and it promotes folding of RNAs by loosening their structures. To understand the mode of action of StpA, we analysed the relationship of its RNA chaperone activity to its RNA-binding properties. For acceleration of annealing of two short RNA...

journal_title：Nucleic acids research

authors： Mayer O,Rajkowitsch L,Lorenz C,Konrat R,Schroeder R

更新日期：2007-01-01 00:00:00

abstract：:A 34 base-pair (bp) fragment spanning sequences -154 to -120 of the promoter of the CHO1 gene (structural gene for phosphatidylserine synthase) from the yeast Saccharomyces cerevisiae has been shown to place transcription of a promoter-less Escherichia coli lacZ gene under control of the phospholipid precursors inosit...

journal_title：Nucleic acids research

authors： Bailis AM,Lopes JM,Kohlwein SD,Henry SA

更新日期：1992-03-25 00:00:00

abstract：:Cell cycle checkpoints induced by DNA damage play an integral role in preservation of genomic stability by allowing cells to limit the propagation of deleterious mutations. The retinoblastoma tumor suppressor (RB) is crucial for the maintenance of the DNA damage checkpoint function because it elicits cell cycle arrest...

journal_title：Nucleic acids research

authors： Bosco EE,Mayhew CN,Hennigan RF,Sage J,Jacks T,Knudsen ES

更新日期：2004-01-02 00:00:00

abstract：:The noncoding RNA database (ncRNAdb) was created as a source of information on RNA molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to constitutively expressed, housekeeping or infrastructural RNAs, there is a wide variety of RNAs participating in mechanisms involved...

journal_title：Nucleic acids research

authors： Szymanski M,Erdmann VA,Barciszewski J

更新日期：2007-01-01 00:00:00

abstract：:Analysis of large-scale gene expression studies usually begins with gene clustering. A ubiquitous problem is that different algorithms applied to the same data inevitably give different results, and the differences are often substantial, involving a quarter or more of the genes analyzed. This raises a series of import...

journal_title：Nucleic acids research

authors： Hart CE,Sharenbroich L,Bornstein BJ,Trout D,King B,Mjolsness E,Wold BJ

更新日期：2005-05-10 00:00:00

abstract：:CAG repeats occur predominantly in the coding regions of human genes, which suggests their functional importance. In some genes, these sequences can undergo pathogenic expansions leading to neurodegenerative polyglutamine (poly-Q) diseases. The mutant transcripts containing expanded CAG repeats possibly contribute to ...

journal_title：Nucleic acids research

authors： Kiliszek A,Kierzek R,Krzyzosiak WJ,Rypniewski W

更新日期：2010-12-01 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated Cas proteins comprise a prokaryotic RNA-guided adaptive immune system that interferes with mobile genetic elements, such as plasmids and phages. The type I-E CRISPR interference complex Cascade from Escherichia coli is composed of...

journal_title：Nucleic acids research

authors： Beloglazova N,Kuznedelov K,Flick R,Datsenko KA,Brown G,Popovic A,Lemak S,Semenova E,Severinov K,Yakunin AF

更新日期：2015-01-01 00:00:00

abstract：:Essential genes are genes that are indispensable to support cellular life. These genes constitute a minimal gene set required for a living cell. We have constructed a Database of Essential Genes (DEG), which contains all the essential genes that are currently available. The functions encoded by essential genes are con...

journal_title：Nucleic acids research

authors： Zhang R,Ou HY,Zhang CT

更新日期：2004-01-01 00:00:00

abstract：:The essential and universal N(6)-threonylcarbamoyladenosine (t(6)A) modification at position 37 of ANN-decoding tRNAs plays a pivotal role in translational fidelity through enhancement of the cognate codon recognition and stabilization of the codon-anticodon interaction. In Escherichia coli, the YgjD (TsaD), YeaZ (Tsa...

journal_title：Nucleic acids research

authors： Zhang W,Collinet B,Perrochia L,Durand D,van Tilbeurgh H

更新日期：2015-02-18 00:00:00

abstract：:High performance liquid chromatography analysis of imidazole open ring 7-methylguanine, 2-6 diamino-4-hydroxy-5N-methyl-formamidopyrimidine (rom7G), showed two well-separated peaks (fI and fII) of the same magnitude. Rechromatography of each isolated component indicated that they are slowly interconverted to give a 1:...

journal_title：Nucleic acids research

authors： Boiteux S,Belleney J,Roques BP,Laval J

更新日期：1984-07-11 00:00:00

abstract：:We have used in vitro transcription in isolated pea nuclei to determine whether the expression of genes for the seed storage proteins legumin and vicilin is regulated transcriptionally or post-transcriptionally in response to changing sulfur status in the developing seed. During the first 48 h of recovery from S defic...

journal_title：Nucleic acids research

authors： Beach LR,Spencer D,Randall PJ,Higgins TJ

更新日期：1985-02-11 00:00:00

abstract：:H/ACA small nucleolar RNAs (snoRNAs) guide pseudouridylation as part of a small nucleolar ribonucleoprotein complex (snoRNP). Disruption of H/ACA snoRNA levels in stem cells impairs pluripotency, yet it remains unclear how H/ACA snoRNAs contribute to differentiation. To determine if H/ACA snoRNA levels are dynamic dur...

journal_title：Nucleic acids research

authors： McCann KL,Kavari SL,Burkholder AB,Phillips BT,Hall TMT

更新日期：2020-09-04 00:00:00

abstract：:The expression of the nicotinic acetylcholine receptor alpha 2 subunit gene is highly restricted to the Spiriform lateralis nucleus of the Chick diencephalon. As a first step toward understanding the molecular mechanism underlying this regulation, we have investigated the structural and regulatory properties of the 5'...

journal_title：Nucleic acids research

authors： Bessis A,Savatier N,Devillers-Thiéry A,Bejanin S,Changeux JP

更新日期：1993-05-11 00:00:00

abstract：:Gene losses provide an insightful route for studying the morphological and physiological adaptations of species, but their discovery is challenging. Existing genome annotation tools focus on annotating intact genes and do not attempt to distinguish nonfunctional genes from genes missing annotation due to sequencing an...

journal_title：Nucleic acids research

authors： Turakhia Y,Chen HI,Marcovitz A,Bejerano G

更新日期：2020-09-18 00:00:00

abstract：:The satellite RNA of tobacco ringspot virus (STobRV RNA) replicates and becomes encapsidated in association with tobacco ringspot virus. Previous results show that the infected tissue produces multimeric STobRV RNAs of both polarities. RNA that is complementary to encapsidated STobRV RNA, designated as having the (-) ...

journal_title：Nucleic acids research

authors： Buzayan JM,Hampel A,Bruening G

更新日期：1986-12-22 00:00:00

abstract：:The numerous genomic sequences and ESTs released by the Arabidopsis thaliana Genome Initiative (AGI) have allowed a systematic and functional study of the DEAD box RNA helicase family. Sequencing and in silico analysis led to the characterization of 28 novel A. thaliana DEAD box RNA helicases forming a family of 32 me...

journal_title：Nucleic acids research

authors： Aubourg S,Kreis M,Lecharny A

更新日期：1999-01-15 00:00:00

abstract：:The -1 ribosomal frameshifting requires the existence of an in cis RNA slippery sequence and is promoted by a downstream stimulator RNA. An atypical RNA pseudoknot with an extra stem formed by complementary sequences within loop 2 of an H-type pseudoknot is characterized in the severe acute respiratory syndrome corona...

journal_title：Nucleic acids research

authors： Su MC,Chang CT,Chu CH,Tsai CH,Chang KY

更新日期：2005-07-29 00:00:00

abstract：:MAGIA(2) (http://gencomp.bio.unipd.it/magia2) is an update, extension and evolution of the MAGIA web tool. It is dedicated to the integrated analysis of in silico target prediction, microRNA (miRNA) and gene expression data for the reconstruction of post-transcriptional regulatory networks. miRNAs are fundamental post...

journal_title：Nucleic acids research

authors： Bisognin A,Sales G,Coppe A,Bortoluzzi S,Romualdi C

更新日期：2012-07-01 00:00:00

abstract：:A hybrid plasmid was constructed containing beta-lactamase gene of plasmid pBR322 and cloned coding sequences of bovine growth hormone (BGH). The constructed plasmid contains all DNA sequences required to encode BGH, and when used as a hybridization probe it detects one growth hormone gene in the bovine genome. The cl...

journal_title：Nucleic acids research

authors： Keshet E,Rosner A,Bernstein Y,Gorecki M,Aviv H

更新日期：1981-01-10 00:00:00

abstract：:We have sequenced the external transcribed spacer (ETS) of a ribosomal transcription unit from Xenopus laevis, together with sections of the preceding non-transcribed spacer. Our analysis was carried out on the same cloned transcription unit as that from which the internal transcribed spacers (ITS) were previously seq...

journal_title：Nucleic acids research

authors： Maden BE,Moss M,Salim M

更新日期：1982-04-10 00:00:00