Abstract：
:DNA bearing lambda operator sequences was methylated by dimethyl sulfate (DMS) in the presence or absence of lambda repressor. Under the experimental conditions, DMS methylates only the purine residues. The presence of lambda repressor affects only the methylation of certain G residues in the operators. Repressor blocks the methylation of certain G's and enhances the methylation of other G's. Since the reactive ring-nitrogen of G lies in the major groove of double-stranded DNA, and the reactive ring-nitrogen of A lies in the minor groove, the above results imply that the repressor makes contacts in the major groove of the helix. The repressor effect on G-methylation is sharply confined to the three 17 base pair units within each lambda operator previously proposed as the repressor-binding sites.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Humayun Z,Kleid D,Ptashne Mdoi
10.1093/nar/4.5.1595subject
Has Abstractpub_date
1977-01-01 00:00:00pages
1595-607issue
5eissn
0305-1048issn
1362-4962journal_volume
4pub_type
杂志文章abstract：:SINE-VNTR-Alu (SVA) elements are non-autonomous, hominid-specific non-LTR retrotransposons and distinguished by their organization as composite mobile elements. They represent the evolutionarily youngest, currently active family of human non-LTR retrotransposons, and sporadically generate disease-causing insertions. S...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr863
更新日期：2012-02-01 00:00:00
abstract：:The nucleotide sequence of the 5S ribosomal RNA of Streptococcus cremoris has been determined. The sequence is 5' (sequence in text) 3'. Comparison of the S. cremoris 5S RNA sequence to an updated prokaryotic generalized 5S RNA structural model shows that this 5S RNA contains some unusual structural features. These fe...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.21.7569
更新日期：1983-11-11 00:00:00
abstract：:A highly repetitive DNA sequence from tilapia (Oreochromis mossambicus/hornorum) has been cloned and sequenced. It is a tandemly arrayed sequence of 237 bp and constitutes 7% of the fish genome. The copy number of the repeat is approximately 3 x 10(5) per haploid genome. DNA sequence analysis of 7 cloned repeats revea...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.13.5071
更新日期：1989-07-11 00:00:00
abstract：:Signal transducer and activator of transcription 6 (STAT6) is a regulator of transcription for interleukin-4 (IL-4)-induced genes. The ability of STAT6 to activate transcription depends on functional interaction with other transcription factors and coactivators. We have characterized the mechanism of STAT6-mediated tr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl539
更新日期：2006-01-01 00:00:00
abstract：:Mammalian DNA (cytosine-5) methyltransferase 1 (DNMT1) is essential for maintenance methylation. Phosphorylation of Ser143 (pSer143) stabilizes DNMT1 during DNA replication. Here, we show 14-3-3 is a reader protein of DNMT1pSer143. In mammalian cells 14-3-3 colocalizes and binds DNMT1pSer143 post-DNA replication. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1162
更新日期：2016-02-29 00:00:00
abstract：:The excision of specific DNA sequences from integrated transgenes in insects permits the dissection in situ of structural elements that may be important in controlling gene expression. Furthermore, manipulation of potential control elements in the context of a single integration site mitigates against insertion site i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng148
更新日期：2003-11-15 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.4.739
更新日期：1990-02-25 00:00:00
abstract：:PlantGDB (http://www.plantgdb.org/) is a genomics database encompassing sequence data for green plants (Viridiplantae). PlantGDB provides annotated transcript assemblies for >100 plant species, with transcripts mapped to their cognate genomic context where available, integrated with a variety of sequence analysis tool...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm1041
更新日期：2008-01-01 00:00:00
abstract：:The Genomes Online Database (GOLD) (https://gold.jgi.doe.gov) is a manually curated data management system that catalogs sequencing projects with associated metadata from around the world. In the current version of GOLD (v.6), all projects are organized based on a four level classification system in the form of a Stud...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw992
更新日期：2017-01-04 00:00:00
abstract：:A restriction endonuclease, ApaI, has been partially purified from Acetobacter pasteurianus. This enzyme cleaves bacteriophage lambda DNA and Simian virus 40 DNA at one site, adenovirus-2 DNA at more than nine sites, but it does not cleave phi X174 DNA nor plasmid pBR322 DNA. This enzyme recognizes the sequence (formu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.13.4409
更新日期：1983-07-11 00:00:00
abstract：:Single-nucleotide polymorphisms (SNPs) are often linked to critical phenotypes such as diseases or responses to vaccines, medications and environmental factors. However, the specific molecular mechanisms by which a causal SNP acts is usually not obvious. Changes in RNA secondary structure emerge as a possible explanat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1009
更新日期：2013-01-07 00:00:00
abstract：:The transcription factor HIV-TF1, which binds to a region about 60 bp upstream from the enhancer of the human immunodeficiency virus-1 (HIV-1), was purified from human B cells. HIV-TF1 had a molecular weight of 39,000. Binding of HIV-TF1 to the HIV long terminal repeat (LTR) activated transcription from the HIV promot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.17.4689
更新日期：1991-09-11 00:00:00
abstract：:A comparison of gene structure, sequence, and transcription pattern of heat shock loci 93D of Drosophila melanogaster and 48B of Drosophila hydei has been performed. Both heat shock loci consist of an unique region that is flanked by an internally repetitive element. Different members of these elements are highly cons...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.8.3317
更新日期：1987-04-24 00:00:00
abstract：:The Eukaryotic Promoter Database (EPD) is an annotated non-redundant collection of eukaryotic POL II promoters, experimentally defined by a transcription start site (TSS). Access to promoter sequences is provided by pointers to positions in the corresponding genomes. Promoter evidence comes from conventional TSS mappi...
journal_title：Nucleic acids research
pub_type： 历史文章,杂志文章
doi：10.1093/nar/gkj146
更新日期：2006-01-01 00:00:00
abstract：:DNA and RNA guanine-quadruplexes (G4s) are stabilized by several cations, in particular by potassium and sodium ions. Generally, potassium stabilizes guanine-quartet assemblies to a larger extent than sodium; in this article we report about a higher-order G4 structure more stable in sodium than in potassium. Repeats o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw003
更新日期：2016-04-07 00:00:00
abstract：:Aging research is a multi-disciplinary field encompassing knowledge from many areas of basic, applied and clinical research. Age-related processes occur on molecular, cellular, tissue, organ, system, organismal and even psychological levels, trigger the onset of multiple debilitating diseases and lead to a loss of fun...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1287
更新日期：2016-01-04 00:00:00
abstract：:Nuclear actin-related proteins (Arps) are subunits of several chromatin remodelers, but their molecular functions within these complexes are unclear. We report the crystal structure of the INO80 complex subunit Arp8 in its ATP-bound form. Human Arp8 has several insertions in the conserved actin fold that explain its i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks842
更新日期：2012-11-01 00:00:00
abstract：:The mouse c-Ki-ras protooncogene promoter contains an unusual DNA element consisting of a 27 bp-long homopurine-homopyrimidine mirror repeat (H-motif) adjacent to a d(C-G)5 repeat. We have previously shown that in vitro these repeats may adopt H and Z conformations, respectively, causing nuclease and chemical hypersen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.16.3271
更新日期：1994-08-25 00:00:00
abstract：:Aberration within the p53 tumor suppressor gene is the most frequently identified genetic damage in human cancer. Regulatory functions proposed for the p53 protein include modulation of the cell cycle, cellular differentiation, signal transduction, and gene expression. Additionally, the p53 gene product may guard the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/21.3.713
更新日期：1993-02-11 00:00:00
abstract：:Anti-silencing function 1 (Asf1) and Chromatin Assembly Factor 1 (CAF-1) chaperone histones H3/H4 during the assembly of nucleosomes on newly replicated DNA. To understand the mechanism of histone H3/H4 transfer among Asf1, CAF-1 and DNA from a thermodynamic perspective, we developed and employed biophysical approache...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks906
更新日期：2012-12-01 00:00:00
abstract：:Helicase loading at a DNA replication origin often requires the dynamic interactions between the DNA helicase and an accessory protein. In E. coli, the DNA helicase is DnaB and DnaC is its loading partner. We used the method of hydrogen/deuterium exchange mass spectrometry to address the importance of DnaB-DnaC comple...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv961
更新日期：2016-01-08 00:00:00
abstract：:The nucleotide sequences of the Streptomyces transposable element IS110 and its insertion site in the DNA of a derivative of the temperate phage luminal diameter C31 were determined. The element is inserted about 460 bp from the right-hand end of luminal diameter C31 DNA, in a region of apparently non-coding DNA. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.17.7053
更新日期：1987-09-11 00:00:00
abstract：:The nucleotide sequence of the Anacystis nidulans 23S rRNA gene, including the 5'- and 3'-flanking regions has been determined. The gene is 2876 nucleotides long and shows higher primary sequence homology to the 23S rRNAs of plastids (84.5%) than to that of E. coli (79%). The predicted rRNA transcript also shares many...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.7.3373
更新日期：1984-04-11 00:00:00
abstract：:A UCSD Pascal program was developed which can analyze nucleic acid dot matrices of up to 9500 X 9500 in size on the Apple II computer. Although matrices of such size consume large amounts of computer memory, this program minimizes these problems by analyzing only small strips of the matrix at a time, and then transfer...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.1part2.767
更新日期：1984-01-11 00:00:00
abstract：:N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/6.2.733
更新日期：1979-02-01 00:00:00
abstract：:Cell differentiation is a central process in development and in cancer growth and dissemination. OCT4 (POU5F1) and NANOG are essential for cell stemness and pluripotency; yet, the mechanisms that regulate their expression remain largely unknown. Repetitive elements account for almost half of the Human Genome; still, t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw095
更新日期：2016-06-02 00:00:00
abstract：:Earlier studies in DNA self-assembly have foretold the feasibility of building addressable nanostructures with multi-stranded motifs, which is fully validated in this study. In realizing this feasibility in DNA nanotechnology, a diversified set of motifs of modified domain lengths is extended from a classic type. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx121
更新日期：2017-04-07 00:00:00
abstract：:Three novel DNA-binding proteins with apparent molecular masses of 7, 10 and 30 kDa have been isolated from the hyperthermophilic methanogen Methanopyrus kandleri. The proteins were identified using a blot overlay assay that was modified to emulate the high ionic strength intracellular environment of M.kandleri protei...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.3.685
更新日期：2002-02-01 00:00:00
abstract：:The structure-specific Mus81-Eme1/Mms4 endonuclease contributes importantly to DNA repair and genome integrity maintenance. Here, using budding yeast, we have studied its function and regulation during the cellular response to DNA damage and show that this endonuclease is necessary for successful chromosome replicatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt645
更新日期：2013-10-01 00:00:00
abstract：:During protein synthesis, the nascent peptide chain traverses the peptide exit tunnel of the ribosome. We monitor the co-translational movement of the nascent peptide using a fluorescent probe attached to the N-terminus of the nascent chain. Due to fluorophore quenching, the time-dependent fluorescence signal emitted ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky740
更新日期：2018-12-14 00:00:00
