Abstract:
:N-(guanosin-8-yl) acetylaminofluorene (Guo-AAF) was prepared by the reaction of N-acetoxy-N-2-acetylaminofluorene (AAAF) and guanosine. Antibodies to Guo-AAF were elicited in rabbits by immunization with bovine serum albumin-Guo-AAF conjugate. The antibodies were purified by affinity chromatography on a Sepharose-Guo-AAF column. The reactivity of these antibodies towards several ligands was studied by radioimmunoassay. The antibodies have the same affinity for double stranded DNA-AAF and single stranded DNA-AAF. Thus the geometry of the regions of DNA substituted by AAF residues is the same in native and denatured DNA. The affinity of the antibodies is smaller for DNA-AAF than for Guo-AAF. This can be due in part to the stacking of AAF residues with the adjacent bases as shown by the study of the interactions between the antibodies and AAF-oligonucleotides. The circular dichroism spectra of AAF-oligonucleotides bound to the antibodies are reported.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Guigues M,Leng Mdoi
10.1093/nar/6.2.733subject
Has Abstractpub_date
1979-02-01 00:00:00pages
733-44issue
2eissn
0305-1048issn
1362-4962journal_volume
6pub_type
杂志文章abstract::CDK12 (cyclin-dependent kinase 12) is a regulatory kinase with evolutionarily conserved roles in modulating transcription elongation. Recent tumor genome studies of breast and ovarian cancers highlighted recurrent CDK12 mutations, which have been shown to disrupt DNA repair in cell-based assays. In breast cancers, CDK...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx187
更新日期:2017-06-20 00:00:00
abstract::In Pseudomonas aeruginosa the RNA chaperone Hfq and the catabolite repression control protein (Crc) act as post-transcriptional regulators during carbon catabolite repression (CCR). In this regard Crc is required for full-fledged Hfq-mediated translational repression of catabolic genes. RNAseq based transcriptome anal...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1245
更新日期:2018-02-16 00:00:00
abstract::The two genes encoding the class IIS restriction-modification system MboII from Moraxella bovis were cloned separately in two compatible plasmids and expressed in E. coli RR1 delta M15. The nucleotide sequences of the MboII endonuclease (R.MboII) and methylase (M.MboII) genes were determined and the putative start cod...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.5.1007
更新日期:1991-03-11 00:00:00
abstract::Improving the accuracy of prediction of gene starts is one of a few remaining open problems in computer prediction of prokaryotic genes. Its difficulty is caused by the absence of relatively strong sequence patterns identifying true translation initiation sites. In the current paper we show that the accuracy of gene s...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.12.2607
更新日期:2001-06-15 00:00:00
abstract::Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelic...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.7.2897
更新日期:1988-04-11 00:00:00
abstract::The last few years have witnessed significant changes in Pfam (https://pfam.xfam.org). The number of families has grown substantially to a total of 17,929 in release 32.0. New additions have been coupled with efforts to improve existing families, including refinement of domain boundaries, their classification into Pfa...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2019-01-08 00:00:00
abstract::The nucleotide sequence for human argininosuccinate synthetase cDNA was determined by analysis of six clones isolated from a single experiment. The sequence covered 1623 nucleotides including 76 bases of poly(A) and contained a 1236 nucleotide open reading frame encoding a protein of 46,434 daltons. In one cDNA isolat...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.18.6505
更新日期:1983-09-24 00:00:00
abstract::We have synthesized the tetradecamer GAGCXGATAACAAG containing a part of the sequence of the lactose operator. A guanine base in the sequence is replaced by the adduct of the carcinogen 2-acetylaminofluorene with guanine. Under the standard conditions of de-protection, the fluorene moiety is lost, leaving behind a gua...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.15.5093
更新日期:1983-08-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2018-07-06 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1986-01-24 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr455
更新日期:2011-09-01 00:00:00
abstract::DNA synthesis by phage T4 DNA polymerase is arrested at specific sequences in single-stranded DNA templates. To determine whether or not T4 DNA polymerase accessory proteins 32, 44, 45 and 62 eliminated recognition of these arrest sites, unique primer-templates were constructed in which DNA synthesis began at a DNA pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.8.3343
更新日期:1986-04-25 00:00:00
abstract::Transcription is the first step in gene expression, and it is the step at which most of the regulation of expression occurs. Although sequenced prokaryotic genomes provide a wealth of information, transcriptional regulatory networks are still poorly understood using the available genomic information, largely because a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr795
更新日期:2012-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa813
更新日期:2020-11-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gky712
更新日期:2018-09-28 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg667
更新日期:2003-08-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks450
更新日期:2012-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.20.4215
更新日期:2001-10-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/14.18.7305
更新日期:1986-09-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-02-28 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:1994-09-01 00:00:00
abstract::Methods for increasing the transient level of expression of transfected DNA in cultured Drosophila cells have been examined. Here we show that cells exposed to the steroid hormone 20-hydroxyecdysone for 48h prior to transfection show an 4 to 5 fold increase in the levels of expression of transfected DNA. By analysis, ...
journal_title:Nucleic acids research
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更新日期:1987-11-25 00:00:00
abstract::TERC is an RNA component of telomerase. However, TERC is also ubiquitously expressed in most human terminally differentiated cells, which don't have telomerase activity. The function of TERC in these cells is largely unknown. Here, we report that TERC enhances the expression and secretion of inflammatory cytokines by ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz584
更新日期:2019-09-05 00:00:00
abstract::We report here on a quantitative technique called COBRA to determine DNA methylation levels at specific gene loci in small amounts of genomic DNA. Restriction enzyme digestion is used to reveal methylation-dependent sequence differences in PCR products of sodium bisulfite-treated DNA as described previously. We show t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.12.2532
更新日期:1997-06-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku490
更新日期:2014-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw105
更新日期:2016-06-02 00:00:00
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journal_title:Nucleic acids research
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更新日期:1984-01-11 00:00:00
abstract::The Schizosaccharomyces pombe genome is one of the smallest among the free-living eukaryotes. We further reduced the S. pombe gene number by large-scale gene deletion to identify a minimal gene set required for growth under laboratory conditions. The genome-reduced strain has four deletion regions: 168.4 kb in the lef...
journal_title:Nucleic acids research
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更新日期:2013-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.22.6047
更新日期:1981-11-25 00:00:00