USF-related transcription factor, HIV-TF1, stimulates transcription of human immunodeficiency virus-1.

Abstract:

:The transcription factor HIV-TF1, which binds to a region about 60 bp upstream from the enhancer of the human immunodeficiency virus-1 (HIV-1), was purified from human B cells. HIV-TF1 had a molecular weight of 39,000. Binding of HIV-TF1 to the HIV long terminal repeat (LTR) activated transcription from the HIV promoter in vitro. The HIV-TF1-binding site in HIV LTR was similar to the site recognized by upstream stimulatory factor (USF) in the adenovirus major late promoter. DNA-binding properties of HIV-TF1 suggested that HIV-TF1 might be identical or related to USF. Interestingly, treatment of purified HIV-TF1 by phosphatase greatly reduced its DNA-binding activity, suggesting that phosphorylation of HIV-TF1 was essential for DNA binding. The disruption of HIV-TF1-binding site induced a 60% decrease in the level of transcription from the HIV promoter in vivo. These results suggest that HIV-TF1 is involved in transcriptional regulation of HIV-1.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Maekawa T,Sudo T,Kurimoto M,Ishii S

doi

10.1093/nar/19.17.4689

keywords:

subject

Has Abstract

pub_date

1991-09-11 00:00:00

pages

4689-94

issue

17

eissn

0305-1048

issn

1362-4962

journal_volume

19

pub_type

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