Abstract:
:We present the preparation of an ion exchange paper which can be used as a solid carrier in the transfer of RNA from gels. In addition to detection by blot hybridization to specific probes, transferred RNA can be characterized by cell-free translation in situ. Preparation of the paper is simple, inexpensive and reproducible. Examples of applications are shown and possible other applications are discussed.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Saris CJ,Franssen HJ,Heuyerjans JH,van Eenbergen J,Bloemers HPdoi
10.1093/nar/10.16.4831subject
Has Abstractpub_date
1982-08-25 00:00:00pages
4831-43issue
16eissn
0305-1048issn
1362-4962journal_volume
10pub_type
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journal_title:Nucleic acids research
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abstract::The DNA sequence of a 354 basepair EcoRI-HindIII fragment of plasmid pMB9 which has originally been derived from plasmid pSC101 has been resolved. This fragment contains a promoter for transcription directed towards the EcoRI site. Escherichia coli RNA polymerase binds to a region within the EcoRI-HindIII fragment whi...
journal_title:Nucleic acids research
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doi:10.1093/nar/8.7.1535
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journal_title:Nucleic acids research
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doi:10.1093/nar/25.4.787
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abstract::The nucleotide sequences 3' to the translational termination codons in a collection of human genes have been analysed for evidence of a preferred 3' context for natural UAG codons. The aim was to see whether human UAG contexts can be related to the recent demonstration of the effects of 3' context on nonsense suppress...
journal_title:Nucleic acids research
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abstract::Long-range enhancers of transcription are a key component of the genomic regulatory architecture. Recent studies have identified bi-directionally transcribed RNAs emanating from these enhancers known as eRNAs. However, it remains unclear how tightly coupled eRNA production is with enhancer activity. Through our system...
journal_title:Nucleic acids research
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abstract::The purpose of this work was to analyse at the molecular level the DNA recognized by the antibodies to Z-DNA in in situ experiments. Antibodies to Z-DNA interact strongly with R-band positive heterochromatic segments of fixed metaphase chromosomes of Cebus (Viegas-Pequignot et al., 1983). These segments are constitute...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1289
更新日期:2015-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.24.8677
更新日期:1983-12-20 00:00:00
abstract::We have prepared several novel phosphoramidites and have synthesised oligonucleotides incorporating them internally. The presence of these residues in an oligonucleotide template presents an impossible barrier to primed synthesis by Taq DNA polymerase. When extended as polymerase chain reaction products, these oligonu...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.24.6805
更新日期:1991-12-25 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkv1313
更新日期:2016-01-04 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1983-02-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-02-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2015-05-26 00:00:00
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journal_title:Nucleic acids research
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更新日期:2019-01-08 00:00:00
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journal_title:Nucleic acids research
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更新日期:2012-12-01 00:00:00