HuD binds to three AU-rich sequences in the 3'-UTR of neuroserpin mRNA and promotes the accumulation of neuroserpin mRNA and protein.


:Neuroserpin is an axonally secreted serine protease inhibitor expressed in the nervous system that protects neurons from ischemia-induced apoptosis. Mutant neuroserpin forms have been found polymerized in inclusion bodies in a familial autosomal encephalopathy causing dementia, or associated with epilepsy. Regulation of neuroserpin expression is mostly unknown. Here we demonstrate that neuroserpin mRNA and the RNA-binding protein HuD are co-expressed in the rat central nervous system, and that HuD binds neuroserpin mRNA in vitro with high affinity. Gel-shift, supershift and T1 RNase assays revealed three HuD-binding sequences in the 3'-untranslated region (3'-UTR) of neuroserpin mRNA. They are AU-rich and 20, 51 and 19 nt in length. HuD binding to neuroserpin mRNA was also demonstrated in extracts of PC12 pheochromocytoma cells. Additionally, ectopic expression of increasing amounts of HuD in these cells results in the accumulation of neuroserpin 3'-UTR mRNA. Furthermore, stably transfected PC12 cells over-expressing HuD contain increased levels of both neuroserpin mRNAs (3.0 and 1.6 kb) and protein. Our results indicate that HuD stabilizes neuroserpin mRNA by binding to specific AU-rich sequences in its 3'-UTR, which prolongs the mRNA lifetime and increases protein level.


Nucleic Acids Res


Nucleic acids research


Cuadrado A,Navarro-Yubero C,Furneaux H,Kinter J,Sonderegger P,Muñoz A





Has Abstract


2002-05-15 00:00:00












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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kitts PA,Ayres MD,Possee RD

    更新日期:1990-10-11 00:00:00

  • DNase I-defined chromatin configuration of the human CD3 gene cluster.

    abstract::The three CD3 genes on human chromosome 11q23 encode proteins (gamma, delta and epsilon) which form part of the antigen receptor on T lymphocytes. All three genes are clustered within 50 kb and are activated approximately contemporaneously during the early stages of T cell ontogeny. In order to pinpoint potential regu...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tunnacliffe A

    更新日期:1990-02-11 00:00:00

  • Solution structure of duplex DNA containing an extrahelical abasic site analog determined by NMR spectroscopy and molecular dynamics.

    abstract::Translesional DNA synthesis past abasic sites proceeds with the preferential incorporation of dAMP opposite the lesion and, depending on the sequence context, one or two base deletions. High-resolution NMR spectroscopy and molecular dynamics simulations were used to determine the three-dimensional structure of a DNA h...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lin Z,Hung KN,Grollman AP,de los Santos C

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  • Determination of the number of superhelical turns by the hyperchromicity of partially denatured covalently-closed DNA molecules.

    abstract::A novel method of determining the number of superhelical turns of a covalently-closed plasmid DNA is described. It relies on the determination of the hyperchromicity, and hence the proportion of unstacked basepairs, of a partially heat-denatured sample which co-migrates during electrophoresis with nicked circular dupl...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Dougherty G,Koller T

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  • The initiation accuracy of the SV40 early transcription is determined by the functional domains of two TATA elements.

    abstract::To locate the boundaries of the TATA element in the SV40 early promoter, point mutations have been constructed such as to cover the whole T + A-rich region of the replication origin. The effects of these mutations on the rate of transcription in vivo show that this region actually contains two TATA elements I and II, ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Pauly M,Treger M,Westhof E,Chambon P

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  • Defining the sequence recognized with BmFTZ-F1, a sequence specific DNA binding factor in the silkworm, Bombyx mori, as revealed by direct sequencing of bound oligonucleotides and gel mobility shift competition analysis.

    abstract::BmFTZ-F1 is a Bombyx mori homologue of FTZ-F1, a positive regulator of the fushi tarazu gene of Drosophila melanogaster. In order to determine the sequence recognized with this factor, we made three sets of oligonucleotide mixture which contain 4 possible nucleotides at different positions within the previously propos...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ueda H,Hirose S

    更新日期:1991-07-11 00:00:00

  • Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase.

    abstract::Insertion of the T3 DNA polymerase thioredoxin binding domain (TBD) into the distantly related thermostable Taq DNA polymerase at an analogous position in the thumb domain, converts the Taq DNA polymerase from a low processive to a highly processive enzyme. Processivity is dependent on the presence of thioredoxin. The...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Davidson JF,Fox R,Harris DD,Lyons-Abbott S,Loeb LA

    更新日期:2003-08-15 00:00:00

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    abstract::The structure of a ribonuclease III processing signal from bacteriophage T7 was examined by NMR spectroscopy, optical melting, and chemical and enzymatic modification. A 41 nucleotide variant of the T7 R1.1 processing signal has two Watson-Crick base-paired helices separated by an internal loop, consistent with its pr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schweisguth DC,Chelladurai BS,Nicholson AW,Moore PB

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    abstract::The thumb subdomain, located in various family B DNA polymerases in the C-terminal region, has been shown in their crystal structures to move upon binding of DNA, changing its conformation to nearly completely wrap around the DNA. It has therefore been involved in DNA binding. In agreement with this, partial proteolys...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Truniger V,Lázaro JM,Salas M

    更新日期:2004-01-16 00:00:00

  • Identification of iron responsive genes by screening cDNA libraries from suppression subtractive hybridization with antisense probes from three iron conditions.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ye Z,Connor JR

    更新日期:2000-04-15 00:00:00

  • Conservation of DNA curvature signals in regulatory regions of prokaryotic genes.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Jáuregui R,Abreu-Goodger C,Moreno-Hagelsieb G,Collado-Vides J,Merino E

    更新日期:2003-12-01 00:00:00

  • Human transcription factor IIIC contains a polypeptide of 55 kDa specifically binding to Pol III genes.

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schneider HR,Waldschmidt R,Seifart KH

    更新日期:1990-08-25 00:00:00

  • A new member of the plasma protease inhibitor gene family.

    abstract::A 2.1-kb cDNA clone representing a new member of the protease inhibitor family was isolated from a human liver cDNA library. The inhibitor, named human Leuserpin 2 (hLS2), comprises 480 amino acids and contains a leucine residue at its putative reactive center. HLS2 is about 25-28% homologous to three human members of...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ragg H

    更新日期:1986-01-24 00:00:00

  • The complete nucleotide sequence of the I-E alpha d immune response gene.

    abstract::We have isolated and sequenced the complete murine I-E alpha immune response gene of the H-2db haplotype. The I-E alpha d gene consists of 5300 basepairs and is organized into five or possibly six exons that correspond to different domains of the alpha chain. The amino acid sequence deduced from the I-E alpha gene sho...

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    pub_type: 杂志文章


    authors: Hyldig-Nielsen JJ,Schenning L,Hammerling U,Widmark E,Heldin E,Lind P,Servenius B,Lund T,Flavell R,Lee JS,Trowsdale J,Schreier PH,Zablitzky F,Larhammar D,Peterson PA,Rask L

    更新日期:1983-08-11 00:00:00

  • Site-specific DNA binding of nuclear factor I: effect of the spacer region.

    abstract::Nuclear factor I (NFI) is a site-specific DNA binding protein required for the replication of adenovirus type 2 DNA in vitro and in vivo. To study sequence requirements for the interaction of NFI with DNA, we have measured the binding of the protein to a variety of synthetic sites. Binding sites for NFI (FIB sites) we...

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    pub_type: 杂志文章


    authors: Gronostajski RM

    更新日期:1987-07-24 00:00:00

  • Changes in transfer ribonucleic acids of Bacillus subtilis during different growth phases.

    abstract::The transfer ribonucleic acids (tRNAs) of B. subtilis at different growth phases are examined for changes in the composition and the methylation of minor constituents. The composition of the tRNAs indicates about equal amounts of adenosine and uridine, and of guanosine and cytidine. About 3-4 residues are present as m...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Singhal RP,Vold B

    更新日期:1976-05-01 00:00:00

  • A 10S galectin-3-U1 snRNP complex assembles into active spliceosomes.

    abstract::In previous studies, we reported that fractionation of HeLa cell nuclear extracts on glycerol gradients revealed an endogenous ∼10S particle that contained galectin-3 and U1 snRNP and this particle was sufficient to load the galectin polypeptide onto a pre-mRNA substrate. We now document that this interaction between ...

    journal_title:Nucleic acids research

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    authors: Haudek KC,Voss PG,Wang JL,Patterson RJ

    更新日期:2016-07-27 00:00:00

  • An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

    abstract::The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs. ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Smith CL,Matsumoto T,Niwa O,Klco S,Fan JB,Yanagida M,Cantor CR

    更新日期:1987-06-11 00:00:00

  • An interplay of the base excision repair and mismatch repair pathways in active DNA demethylation.

    abstract::Active DNA demethylation (ADDM) in mammals occurs via hydroxylation of 5-methylcytosine (5mC) by TET and/or deamination by AID/APOBEC family enzymes. The resulting 5mC derivatives are removed through the base excision repair (BER) pathway. At present, it is unclear how the cell manages to eliminate closely spaced 5mC ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Grin I,Ishchenko AA

    更新日期:2016-05-05 00:00:00

  • Structural analysis of an RNA molecule involved in replication control of plasmid R1.

    abstract::The replication control circuit of the FII plasmids R1, R100 and R6-5 involves a small untranslated RNA molecule of about 93 nucleotides. This RNA, designated CopA RNA in plasmid R1, acts in vivo as a post-transcriptional inhibitor of the expression of the RepA protein whose synthesis is required for each round of pla...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Gerhart E,Wagner H,Nordström K

    更新日期:1986-03-25 00:00:00

  • The enigmatic mitochondrial ORF ymf39 codes for ATP synthase chain b.

    abstract::ymf39 is a conserved hypothetical protein-coding gene found in mitochondrial genomes of land plants and certain protists. We speculated earlier, based on a weak sequence similarity between Ymf39 from a green alga and the atpF gene product from Bradyrhizobium, that ymf39 might code for subunit b of mitochondrial F(0)F(...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Burger G,Lang BF,Braun HP,Marx S

    更新日期:2003-05-01 00:00:00

  • MethBank 3.0: a database of DNA methylomes across a variety of species.

    abstract::MethBank ( is a database that integrates high-quality DNA methylomes across a variety of species and provides an interactive browser for visualization of methylation data. Here, we present an updated implementation of MethBank (version 3.0) by incorporating more DNA methylomes from multi...

    journal_title:Nucleic acids research

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    authors: Li R,Liang F,Li M,Zou D,Sun S,Zhao Y,Zhao W,Bao Y,Xiao J,Zhang Z

    更新日期:2018-01-04 00:00:00

  • Analysis of rRNA processing and translation in mammalian cells using a synthetic 18S rRNA expression system.

    abstract::Analysis of processing, assembly, and function of higher eukaryotic ribosomal RNA (rRNA) has been hindered by the lack of an expression system that enables rRNA to be modified and then examined functionally. Given the potential usefulness of such a system, we have developed one for mammalian 18S rRNA. We inserted a se...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


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  • BhairPred: prediction of beta-hairpins in a protein from multiple alignment information using ANN and SVM techniques.

    abstract::This paper describes a method for predicting a supersecondary structural motif, beta-hairpins, in a protein sequence. The method was trained and tested on a set of 5102 hairpins and 5131 non-hairpins, obtained from a non-redundant dataset of 2880 proteins using the DSSP and PROMOTIF programs. Two machine-learning tech...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kumar M,Bhasin M,Natt NK,Raghava GP

    更新日期:2005-07-01 00:00:00

  • A novel regulatory circuit in base excision repair involving AP endonuclease 1, Creb1 and DNA polymerase beta.

    abstract::DNA repair is required to maintain genome stability in stem cells and early embryos. At critical junctures, oxidative damage to DNA requires the base excision repair (BER) pathway. Since early zebrafish embryos lack the major polymerase in BER, DNA polymerase ß, repair proceeds via replicative polymerases, even though...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Pei DS,Yang XJ,Liu W,Guikema JE,Schrader CE,Strauss PR

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  • Construction of recombinant DNA by exonuclease recession.

    abstract::We describe a new exonuclease-based method for joining and/or constructing two or more DNA molecules. DNA fragments containing ends complementary to those of a vector or another independent molecules were generated by the polymerase chain reaction. The 3' ends of these molecules as well as the vector DNA were then rec...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Yang YS,Watson WJ,Tucker PW,Capra JD

    更新日期:1993-04-25 00:00:00

  • The Sulfolobus solfataricus radA paralogue sso0777 is DNA damage inducible and positively regulated by the Sta1 protein.

    abstract::Little is known about the regulation of the DNA damage-mediated gene expression in archaea. Here we report that the addition of actinomycin D to Sulfolobus solfataricus cultures triggers the expression of the radA paralogue sso0777. Furthermore, a specific retarded band is observed when electrophoretic mobility shift ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Abella M,Rodríguez S,Paytubi S,Campoy S,White MF,Barbé J

    更新日期:2007-01-01 00:00:00

  • Conformational heterogeneity and bubble dynamics in single bacterial transcription initiation complexes.

    abstract::Transcription initiation is a major step in gene regulation for all organisms. In bacteria, the promoter DNA is first recognized by RNA polymerase (RNAP) to yield an initial closed complex. This complex subsequently undergoes conformational changes resulting in DNA strand separation to form a transcription bubble and ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Duchi D,Gryte K,Robb NC,Morichaud Z,Sheppard C,Brodolin K,Wigneshweraraj S,Kapanidis AN

    更新日期:2018-01-25 00:00:00

  • Synthesis of cyclic oligonucleotides by a modified phosphotriester approach.

    abstract::Evidence will be presented to show that the allyl group is suitable for the protection of a 3'-terminal phosphodiester function. The latter will be demonstrated by the synthesis, via a phosphotriester approach, of two cyclic tetraribonucleotides [r(AAAA) and r(UAMe2UAMe2)], two cyclic hexadeoxyribonucleotides [d(CGCGC...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: de Vroom E,Broxterman HJ,Sliedregt LA,van der Marel GA,van Boom JH

    更新日期:1988-05-25 00:00:00

  • Solution structure and functional importance of a conserved RNA hairpin of eel LINE UnaL2.

    abstract::The eel long interspersed element (LINE) UnaL2 and its partner short interspersed element (SINE) share a conserved 3' tail that is critical for their retrotransposition. The predicted secondary structure of the conserved 3' tail of UnaL2 RNA contains a stem region with a putative internal loop. Deletion of the putativ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Nomura Y,Kajikawa M,Baba S,Nakazato S,Imai T,Sakamoto T,Okada N,Kawai G

    更新日期:2006-01-01 00:00:00