Abstract:
:RNA silencing is an evolutionarily conserved mechanism triggered by double-stranded RNA that is processed into 21- to 24-nt small interfering (si)RNA or micro (mi)RNA by RNaseIII-like enzymes called Dicers. Gene regulations by RNA silencing have fundamental implications in a large number of biological processes that include antiviral defense, maintenance of genome integrity and the orchestration of cell fates. Although most generic or core components of the various plant small RNA pathways have been likely identified over the past 15 years, factors involved in RNAi regulation through post-translational modifications are just starting to emerge, mostly through forward genetic studies. A genetic screen designed to identify factors required for RNAi in Arabidopsis identified the serine/threonine protein kinase, TOUSLED (TSL). Mutations in TSL affect exogenous and virus-derived siRNA activity in a manner dependent upon its kinase activity. By contrast, despite their pleiotropic developmental phenotype, tsl mutants show no defect in biogenesis or activity of miRNA or endogenous trans-acting siRNA. These data suggest a possible role for TSL phosphorylation in the specific regulation of exogenous and antiviral RNA silencing in Arabidopsis and identify TSL as an intrinsic regulator of RNA interference.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Uddin MN,Dunoyer P,Schott G,Akhter S,Shi C,Lucas WJ,Voinnet O,Kim JYdoi
10.1093/nar/gku422subject
Has Abstractpub_date
2014-07-01 00:00:00pages
7971-80issue
12eissn
0305-1048issn
1362-4962pii
gku422journal_volume
42pub_type
杂志文章abstract::Bacterial transcription factors often function as DNA-binding proteins that selectively activate or repress promoters, although the biochemical mechanisms vary. In most well-understood examples, activators function by either increasing the affinity of RNA polymerase (RNAP) for the target promoter, or by increasing the...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr1280
更新日期:2012-04-01 00:00:00
abstract::The interaction of N--Acetyl--Phe--tRNA Phe with 70 S ribosomes is a reversible process in the absence as well as in the presence of messenger. The equilibrium binding constants of these interactions were measured at different magnesium concentrations and temperatures and thermodynamical quantities computed. The entha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.10.3871
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abstract::Myotonic dystrophy type 1 (DM1) is caused by the expansion of a (CTG).(CAG) repeat in the DMPK gene on chromosome 19q13.3. At least 17 neurological diseases have similar genetic mutations, the expansion of DNA repeats. In most of these disorders, the disease severity is related to the length of the repeat expansion, a...
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pub_type: 杂志文章
doi:10.1093/nar/7.6.1541
更新日期:1979-11-24 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.1.206
更新日期:1996-01-01 00:00:00
abstract::The Saccharomyces Genome Database (SGD; http://www.yeastgenome.org) is an expertly curated database of literature-derived functional information for the model organism budding yeast, Saccharomyces cerevisiae. SGD constantly strives to synergize new types of experimental data and bioinformatics predictions with existin...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-01-04 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr011
更新日期:2011-05-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.7.2419
更新日期:1982-04-10 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm173
更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkh315
更新日期:2004-02-27 00:00:00
abstract::In this study, we present the DNA-Binding Site Identifier (DBSI), a new structure-based method for predicting protein interaction sites for DNA binding. DBSI was trained and validated on a data set of 263 proteins (TRAIN-263), tested on an independent set of protein-DNA complexes (TEST-206) and data sets of 29 unbound...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt617
更新日期:2013-09-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh352
更新日期:2004-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv563
更新日期:2015-10-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1098
更新日期:2014-02-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2013-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw142
更新日期:2016-06-20 00:00:00
abstract::The nucleotide sequence of the promoter region and the first five genes of the atp (or unc) operon of Escherichia coli has been determined. The first proposed gene in the operon contains four AUA codons and may be poorly expressed; it encodes a basic but yet hydrophobic protein which could function as a pilot protein ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.16.3919
更新日期:1981-08-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.suppl.2181
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journal_title:Nucleic acids research
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更新日期:1994-08-11 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkx553
更新日期:2017-09-06 00:00:00
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更新日期:2012-02-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2005-03-14 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.14.2774
更新日期:1996-07-15 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1303
更新日期:2014-03-01 00:00:00
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更新日期:1977-08-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2010-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh148
更新日期:2004-11-01 00:00:00