Abstract:
:Two models have been proposed for triggering release of the lagging strand polymerase at the replication fork, enabling cycling to the primer for the next Okazaki fragment--either collision with the 5'-end of the preceding fragment (collision model) or synthesis of a new primer by primase (signaling model). Specific perturbation of lagging strand elongation on minicircles with a highly asymmetric G:C distribution with ddGTP or dGDPNP yielded results that confirmed the signaling model and ruled out the collision model. We demonstrated that the presence of a primer, not primase per se, provides the signal that triggers cycling. Lagging strand synthesis proceeds much faster than leading strand synthesis, explaining why gaps between Okazaki fragments are not found under physiological conditions.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Yuan Q,McHenry CSdoi
10.1093/nar/gkt1098subject
Has Abstractpub_date
2014-02-01 00:00:00pages
1747-56issue
3eissn
0305-1048issn
1362-4962pii
gkt1098journal_volume
42pub_type
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