Cycling of the E. coli lagging strand polymerase is triggered exclusively by the availability of a new primer at the replication fork.

Abstract:

:Two models have been proposed for triggering release of the lagging strand polymerase at the replication fork, enabling cycling to the primer for the next Okazaki fragment--either collision with the 5'-end of the preceding fragment (collision model) or synthesis of a new primer by primase (signaling model). Specific perturbation of lagging strand elongation on minicircles with a highly asymmetric G:C distribution with ddGTP or dGDPNP yielded results that confirmed the signaling model and ruled out the collision model. We demonstrated that the presence of a primer, not primase per se, provides the signal that triggers cycling. Lagging strand synthesis proceeds much faster than leading strand synthesis, explaining why gaps between Okazaki fragments are not found under physiological conditions.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Yuan Q,McHenry CS

doi

10.1093/nar/gkt1098

subject

Has Abstract

pub_date

2014-02-01 00:00:00

pages

1747-56

issue

3

eissn

0305-1048

issn

1362-4962

pii

gkt1098

journal_volume

42

pub_type

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