The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis.


:Genetic recombination in meiosis plays an important role in generating diversity of genetic information. In yeast an Escherichia coli RecA-like gene, DMC1, is expressed in meiotic prophase and its product co-localizes with Rad51 protein on zygotene chromosomes. We have cloned the mouse and human homologs of the yeast DMC1 gene. The predicted human and mouse DMC1 proteins showed 54.1% sequence identity with yeast Dmc1 protein. The domain II region, highly conserved in the E.coli RecA-like protein family, was also found in the mammalian DMC1 proteins, including the two ATP binding motifs and DNA binding sites with the region. In situ hybridization analysis revealed expression of the mouse Dmc1 gene in testicular germ cells in meiosis; RT-PCR showed expression in embryonal ovaries. These findings suggest that DMC1 plays an important role in meiotic homologous recombination. From both the man and mouse we have isolated an alternative spliced form of Dmc1 cDNA (Dmc1-d), which is deleted for a region between the two motifs involved in nucleotide binding. Since the alternatively spliced Dmc1-d transcript was detected in both male and female germ cells, the encoded protein DMC1-D may have a novel role in mammalian genetic recombination in meiosis.


Nucleic Acids Res


Nucleic acids research


Habu T,Taki T,West A,Nishimune Y,Morita T




Has Abstract


1996-02-01 00:00:00














  • Substrate masking: binding of RNA by EGTA-inactivated micrococcal nuclease results in artifactual inhibition of RNA processing reactions.

    abstract::Inhibition of an RNA processing reaction after treatment with the Ca2(+)-dependent micrococcal nuclease (MN) is often used as a criterion for the presence of a required RNA or ribonucleoprotein component in the system. Following MN digestion, the nuclease is inactivated with EGTA and radiolabeled substrate is added to...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wang MJ,Gegenheimer P

    更新日期:1990-11-25 00:00:00

  • GeneAlign: a coding exon prediction tool based on phylogenetical comparisons.

    abstract::GeneAlign is a coding exon prediction tool for predicting protein coding genes by measuring the homologies between a sequence of a genome and related sequences, which have been annotated, of other genomes. Identifying protein coding genes is one of most important tasks in newly sequenced genomes. With increasing numbe...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hsieh SJ,Lin CY,Liu NH,Chow WY,Tang CY

    更新日期:2006-07-01 00:00:00

  • Flexibility and stabilization of HgII-mediated C:T and T:T base pairs in DNA duplex.

    abstract::Owing to their great potentials in genetic code extension and the development of nucleic acid-based functional nanodevices, DNA duplexes containing HgII-mediated base pairs have been extensively studied during the past 60 years. However, structural basis underlying these base pairs remains poorly understood. Herein, w...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Liu H,Cai C,Haruehanroengra P,Yao Q,Chen Y,Yang C,Luo Q,Wu B,Li J,Ma J,Sheng J,Gan J

    更新日期:2017-03-17 00:00:00

  • jpHMM at GOBICS: a web server to detect genomic recombinations in HIV-1.

    abstract::Detecting recombinations in the genome sequence of human immunodeficiency virus (HIV-1) is crucial for epidemiological studies and for vaccine development. Herein, we present a web server for subtyping and localization of phylogenetic breakpoints in HIV-1. Our software is based on a jumping profile Hidden Markov Model...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zhang M,Schultz AK,Calef C,Kuiken C,Leitner T,Korber B,Morgenstern B,Stanke M

    更新日期:2006-07-01 00:00:00

  • Interference activity of a minimal Type I CRISPR-Cas system from Shewanella putrefaciens.

    abstract::Type I CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) systems exist in bacterial and archaeal organisms and provide immunity against foreign DNA. The Cas protein content of the DNA interference complexes (termed Cascade) varies between different CRISPR-Cas subtypes. A minima...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Dwarakanath S,Brenzinger S,Gleditzsch D,Plagens A,Klingl A,Thormann K,Randau L

    更新日期:2015-10-15 00:00:00

  • PharmaGist: a webserver for ligand-based pharmacophore detection.

    abstract::Predicting molecular interactions is a major goal in rational drug design. Pharmacophore, which is the spatial arrangement of features that is essential for a molecule to interact with a specific target receptor, is an important model for achieving this goal. We present a freely available web server, named PharmaGist,...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schneidman-Duhovny D,Dror O,Inbar Y,Nussinov R,Wolfson HJ

    更新日期:2008-07-01 00:00:00

  • Argonaute binding within 3'-untranslated regions poorly predicts gene repression.

    abstract::Despite two decades of study, the full scope of RNAi in mammalian cells has remained obscure. Here we combine: (i) Knockout of argonaute (AGO) variants; (ii) RNA sequencing analysis of gene expression changes and (iii) Enhanced Crosslinking Immunoprecipitation Sequencing (eCLIP-seq) using anti-AGO2 antibody to identif...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chu Y,Kilikevicius A,Liu J,Johnson KC,Yokota S,Corey DR

    更新日期:2020-07-27 00:00:00

  • Structures of m-iodo Hoechst-DNA complexes in crystals with reduced solvent content: implications for minor groove binder drug design.

    abstract::The DNA photosensitisers m-iodo Hoechst and m-iodo, p-methoxy Hoechst have been co-crystallised with the oligonucleotide d(CGCGAATTCGCG)(2)and their crystal structures determined. The crystals were then subjected to slow dehydration, which reduced their solvent contents from 40 (normal) to 30 (partially dehydrated) an...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Squire CJ,Baker LJ,Clark GR,Martin RF,White J

    更新日期:2000-03-01 00:00:00

  • Binding of aminoglycoside antibiotics to helix 69 of 23S rRNA.

    abstract::Aminoglycosides antibiotics negate dissociation and recycling of the bacterial ribosome's subunits by binding to Helix 69 (H69) of 23S rRNA. The differential binding of various aminoglycosides to the chemically synthesized terminal domains of the Escherichia coli and human H69 has been characterized using spectroscopy...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Scheunemann AE,Graham WD,Vendeix FA,Agris PF

    更新日期:2010-05-01 00:00:00

  • Analysis of the 5' regulatory region of the gene for delta-aminolevulinic acid synthetase of Rhizobium meliloti.

    abstract::Transcriptional regulation of the delta-aminolevulinic acid synthetase gene of Rhizobium meliloti was investigated under conditions of normal vegetative growth and during symbiosis with the legume host alfalfa. S1 nuclease mapping and DNA sequence analysis indicated that transcription originates from two sites separat...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Leong SA,Williams PH,Ditta GS

    更新日期:1985-08-26 00:00:00

  • Identification of a novel epigenetic regulatory region within the pluripotency associated microRNA cluster, EEmiRC.

    abstract::The miR-290 cluster is expressed in embryonic stem cells (ESCs) and is important for the maintenance of pluripotency, but little is known about the mechanisms regulating the early embryonic microRNA cluster (EEmiRC) expression. Here we report the identification of a 332-bp intragenic enhancer (IE) able to modulate the...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tata PR,Tata NR,Kühl M,Sirbu IO

    更新日期:2011-05-01 00:00:00

  • Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer.

    abstract::As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Yoshimura K,Rosenfeld MA,Nakamura H,Scherer EM,Pavirani A,Lecocq JP,Crystal RG

    更新日期:1992-06-25 00:00:00

  • Structural dynamics of propeller loop: towards folding of RNA G-quadruplex.

    abstract::We have carried out an extended set of standard and enhanced-sampling MD simulations (for a cumulative simulation time of 620 μs) with the aim to study folding landscapes of the rGGGUUAGGG and rGGGAGGG parallel G-hairpins (PH) with propeller loop. We identify folding and unfolding pathways of the PH, which is bridged ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Havrila M,Stadlbauer P,Kührová P,Banáš P,Mergny JL,Otyepka M,Šponer J

    更新日期:2018-09-28 00:00:00

  • Pituitary-specific chromatin structure of the rat prolactin distal enhancer element.

    abstract::The location of target DNA sequences within chromatin may affect the ability of trans-acting factors to bind cis-elements and regulate gene transcription. To examine the effect of chromatin structure on the ability of the estrogen-estrogen receptor complex (E2R) to bind its respective DNA binding element within the ra...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Willis SD,Seyfred MA

    更新日期:1996-03-15 00:00:00

  • The phosphate clamp: a small and independent motif for nucleic acid backbone recognition.

    abstract::The 1.7 Å X-ray crystal structure of the B-DNA dodecamer, [d(CGCGAATTCGCG)]₂ (DDD)-bound non-covalently to a platinum(II) complex, [{Pt(NH₃)₃}₂-µ-{trans-Pt(NH₃)₂(NH₂(CH₂)₆NH₂)₂}](NO₃)₆ (1, TriplatinNC-A,) shows the trinuclear cation extended along the phosphate backbone and bridging the minor groove. The square planar...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Komeda S,Moulaei T,Chikuma M,Odani A,Kipping R,Farrell NP,Williams LD

    更新日期:2011-01-01 00:00:00

  • Structural basis for single-stranded RNA recognition and cleavage by C3PO.

    abstract::Translin and translin-associated factor-x are highly conserved in eukaroytes; they can form heteromeric complexes (known as C3POs) and participate in various nucleic acid metabolism pathways. In humans and Drosophila, C3POs cleave the fragmented siRNA passenger strands and facilitate the activation of RNA-induced sile...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Zhang J,Liu H,Yao Q,Yu X,Chen Y,Cui R,Wu B,Zheng L,Zuo J,Huang Z,Ma J,Gan J

    更新日期:2016-11-02 00:00:00

  • RaftProt: mammalian lipid raft proteome database.

    abstract::RaftProt ( is a database of mammalian lipid raft-associated proteins as reported in high-throughput mass spectrometry studies. Lipid rafts are specialized membrane microdomains enriched in cholesterol and sphingolipids thought to act as dynamic signalling and sorting platforms....

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Shah A,Chen D,Boda AR,Foster LJ,Davis MJ,Hill MM

    更新日期:2015-01-01 00:00:00

  • Quantitative sampling of conformational heterogeneity of a DNA hairpin using molecular dynamics simulations and ultrafast fluorescence spectroscopy.

    abstract::Molecular dynamics (MD) simulations and time resolved fluorescence (TRF) spectroscopy were combined to quantitatively describe the conformational landscape of the DNA primary binding sequence (PBS) of the HIV-1 genome, a short hairpin targeted by retroviral nucleocapsid proteins implicated in the viral reverse transcr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Voltz K,Léonard J,Touceda PT,Conyard J,Chaker Z,Dejaegere A,Godet J,Mély Y,Haacke S,Stote RH

    更新日期:2016-04-20 00:00:00

  • Nucleotide sequence analysis of a region of adenovirus 5 DNA encoding a hitherto unidentified gene.

    abstract::The nucleotide sequence of a region located on the adenovirus type 5 genome between coordinates 59.9 and 62.5 has been determined. This region comprises the 3'-termini of mRNAs coding for the hexon and the DNA binding protein. The sequence reveals the presence of an open reading frame in r-strand transcripts downstrea...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Kruijer W,van Schaik FM,Sussenbach JS

    更新日期:1980-12-20 00:00:00

  • Comparison of five methods for finding conserved sequences in multiple alignments of gene regulatory regions.

    abstract::Conserved segments in DNA or protein sequences are strong candidates for functional elements and thus appropriate methods for computing them need to be developed and compared. We describe five methods and computer programs for finding highly conserved blocks within previously computed multiple alignments, primarily fo...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Stojanovic N,Florea L,Riemer C,Gumucio D,Slightom J,Goodman M,Miller W,Hardison R

    更新日期:1999-10-01 00:00:00

  • Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) genotype analysis and validation using real-time PCR.

    abstract::With an increased emphasis on genotyping of single nucleotide polymorphisms (SNPs) in disease association studies, the genotyping platform of choice is constantly evolving. In addition, the development of more specific SNP assays and appropriate genotype validation applications is becoming increasingly critical to elu...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Johnson MP,Haupt LM,Griffiths LR

    更新日期:2004-03-26 00:00:00

  • Identification of the ATP-binding site in the terminase subunit pUL56 of human cytomegalovirus.

    abstract::Human cytomegalovirus (HCMV) terminase is composed of subunits pUL56 (130 kDa) and pUL89 ( approximately 75 kDa), encoded by the UL56 and UL89 genes. In a recent investigation, we demonstrated that the main ATPase activity is associated with the large terminase subunit pUL56. The protein has two putative ATP-binding s...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Scholz B,Rechter S,Drach JC,Townsend LB,Bogner E

    更新日期:2003-03-01 00:00:00

  • MERAV: a tool for comparing gene expression across human tissues and cell types.

    abstract::The oncogenic transformation of normal cells into malignant, rapidly proliferating cells requires major alterations in cell physiology. For example, the transformed cells remodel their metabolic processes to supply the additional demand for cellular building blocks. We have recently demonstrated essential metabolic pr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Shaul YD,Yuan B,Thiru P,Nutter-Upham A,McCallum S,Lanzkron C,Bell GW,Sabatini DM

    更新日期:2016-01-04 00:00:00

  • In vitro DNA dependent synthesis of globin RNA sequences from erythroleukemic cell chromatin.

    abstract::Murine erythroleukemic cells in culture accumulate cytoplasmic globin mRNA during differentiation induced by dimethyl sulfoxide (DMSO)1. Chromatin was prepared from DMSO induced erythroleukemic cells that were transcribing globin RNA in order to determine whether in vitro synthesis of globin RNA sequences was possible...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Reff ME,Davidson RL

    更新日期:1979-01-01 00:00:00

  • Joint annotation of coding and non-coding single nucleotide polymorphisms and mutations in the SNPeffect and PupaSuite databases.

    abstract::Single nucleotide polymorphisms (SNPs) are, together with copy number variation, the primary source of variation in the human genome. SNPs are associated with altered response to drug treatment, susceptibility to disease and other phenotypic variation. Furthermore, during genetic screens for disease-associated mutatio...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Reumers J,Conde L,Medina I,Maurer-Stroh S,Van Durme J,Dopazo J,Rousseau F,Schymkowitz J

    更新日期:2008-01-01 00:00:00

  • Involvement of Schizosaccharomyces pombe rrp1+ and rrp2+ in the Srs2- and Swi5/Sfr1-dependent pathway in response to DNA damage and replication inhibition.

    abstract::Previously we identified Rrp1 and Rrp2 as two proteins required for the Sfr1/Swi5-dependent branch of homologous recombination (HR) in Schizosaccharomyces pombe. Here we use a yeast two-hybrid approach to demonstrate that Rrp1 and Rrp2 can interact with each other and with Swi5, an HR mediator protein. Rrp1 and Rrp2 f...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Dziadkowiec D,Kramarz K,Kanik K,Wisniewski P,Carr AM

    更新日期:2013-09-01 00:00:00

  • A screen of chemical modifications identifies position-specific modification by UNA to most potently reduce siRNA off-target effects.

    abstract::Small interfering RNAs (siRNAs) are now established as the preferred tool to inhibit gene function in mammalian cells yet trigger unintended gene silencing due to their inherent miRNA-like behavior. Such off-target effects are primarily mediated by the sequence-specific interaction between the siRNA seed regions (posi...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bramsen JB,Pakula MM,Hansen TB,Bus C,Langkjær N,Odadzic D,Smicius R,Wengel SL,Chattopadhyaya J,Engels JW,Herdewijn P,Wengel J,Kjems J

    更新日期:2010-09-01 00:00:00

  • Effect of 5-methylcytosine on the stability of triple-stranded DNA--a thermodynamic study.

    abstract::We have previously shown that the pyrimidine oligonucleotide 5'CTTCCTCCTCT (Y11) recognizes the double-helical stem of hairpin 5'GAAGGAGGAGA-T4-TCTCCTCCTTC (h26) by triple-helix formation (1). In this paper, we report the effect on triplex formation of substituting the cytosine residues of Y11 with 5-methylcytosines (...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Xodo LE,Manzini G,Quadrifoglio F,van der Marel GA,van Boom JH

    更新日期:1991-10-25 00:00:00

  • Endonucleolytic activation directs dark-induced chloroplast mRNA degradation.

    abstract::Plastid mRNA stability is tightly regulated by external signals such as light. We have investigated the biochemical mechanism responsible for the dark-induced decrease of relative half-lives for mRNAs encoding photosynthetic proteins. Protein fractions isolated from plastids of light-grown and dark-adapted plants corr...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Baginsky S,Gruissem W

    更新日期:2002-10-15 00:00:00

  • The Bgee suite: integrated curated expression atlas and comparative transcriptomics in animals.

    abstract::Bgee is a database to retrieve and compare gene expression patterns in multiple animal species, produced by integrating multiple data types (RNA-Seq, Affymetrix, in situ hybridization, and EST data). It is based exclusively on curated healthy wild-type expression data (e.g., no gene knock-out, no treatment, no disease...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Bastian FB,Roux J,Niknejad A,Comte A,Fonseca Costa SS,de Farias TM,Moretti S,Parmentier G,de Laval VR,Rosikiewicz M,Wollbrett J,Echchiki A,Escoriza A,Gharib WH,Gonzales-Porta M,Jarosz Y,Laurenczy B,Moret P,Person E,

    更新日期:2021-01-08 00:00:00