Dihydropyrimidine amidohydrolases and dihydroorotases share the same origin and several enzymatic properties.

abstract：:In order to direct antisense oligonucleotides to specific tissues or cell types in vivo, we are exploring the possibility to utilize lipoproteins as transport vehicles. A 16-mer oligonucleotide (ODN) was derivatized at the 5' prime through a 32P phosphate spacer with cholesterol, yielding a 32P-labeled amphiphatic cho...

journal_title：Nucleic acids research

authors： de Smidt PC,Le Doan T,de Falco S,van Berkel TJ

更新日期：1991-09-11 00:00:00

abstract：:A highly sensitive method to detect traces of aldehyde-containing apurinic/apyrimidinic (AP) sites in nucleic acids has been developed. Based on this method, a novel approach to detect DNA base mismatches recognized by the mismatch repair glycosylase MutY is demonstrated. Open chain aldehydes generated in nucleic acid...

journal_title：Nucleic acids research

authors： Maulik G,Botchway S,Chakrabarti S,Tetradis S,Price B,Makrigiorgos GM

更新日期：1999-03-01 00:00:00

abstract：:We describe the structure (3840 bp) of a novel Euglena gracilis chloroplast ribosomal protein operon that encodes the five genes rpl16-rpl14-rpl5-rps8-rpl36. The gene organization resembles the spc and the 3'-end of the S10 ribosomal protein operons of E. coli. The rpl5 is a new chloroplast gene not previously reporte...

journal_title：Nucleic acids research

authors： Christopher DA,Hallick RB

更新日期：1989-10-11 00:00:00

abstract：:HORMA domain-containing proteins (HORMADs) play an essential role in meiosis in many organisms. The meiotic HORMADs, including yeast Hop1, mouse HORMAD1 and HORMAD2, and Arabidopsis ASY1, assemble along chromosomes at early prophase and the closure motif at their C-termini has been hypothesized to be instrumental for ...

journal_title：Nucleic acids research

authors： Yang C,Hu B,Portheine SM,Chuenban P,Schnittger A

更新日期：2020-11-18 00:00:00

abstract：:Resistance to androgen receptor (AR)-targeted therapies in prostate cancer (PC) is a major clinical problem. A key mechanism of treatment resistance in advanced PC is the generation of alternatively spliced forms of the AR termed AR variants (AR-Vs) that are refractory to targeted agents and drive tumour progression. ...

journal_title：Nucleic acids research

authors： Kounatidou E,Nakjang S,McCracken SRC,Dehm SM,Robson CN,Jones D,Gaughan L

更新日期：2019-06-20 00:00:00

abstract：:The transcription of ribosomal genes in a cell line (Kc) derived from female D.melanogaster, has been investigated using hybridization probes prepared from restriction fragments of a cloned rDNA repeat with a 5 kb type I [Wellauer et al. (1978) Cell 14, 269-278] intron. Gels, of nuclear RNA that have been transferred ...

journal_title：Nucleic acids research

authors： Jolly DJ,Thomas CA Jr

更新日期：1980-01-11 00:00:00

abstract：:Several lines of evidence indicate that differences in DNA repair capacity are an important source of variability in cancer risk. However, traditional assays for measurement of DNA repair activity in human samples are laborious and time-consuming. DNA glycosylases are the first step in base excision repair of a variet...

journal_title：Nucleic acids research

authors： Collini M,Caccia M,Chirico G,Barone F,Dogliotti E,Mazzei F

更新日期：2005-10-21 00:00:00

abstract：:The solution structures of two 27 nt RNA hairpins and their complexes with cobalt(III)-hexammine [Co(NH(3))(6)(3+)] were determined by NMR spectroscopy. The RNA hairpins are variants of the P4 region from Escherichia coli RNase P RNA: a U-to-A mutant changing the identity of the bulged nucleotide, and a U-to-C, C-to-U...

journal_title：Nucleic acids research

authors： Schmitz M

更新日期：2004-12-02 00:00:00

abstract：:The cnjC gene from the protozoan Tetrahymena thermophila was completely sequenced. The deduced gene product was found to have significant sequence similarity to the yeast and prokaryotic RNA polymerase subunits involved with subunit assembly. Since cnjC is active only during the sexual stage (conjugation) of Tetrahyme...

journal_title：Nucleic acids research

authors： Martindale DW

更新日期：1990-05-25 00:00:00

abstract：:The macronuclear genome of Euplotes crassus contains two different genes, EFA1 and EFA2, encoding EF-1alpha proteins. They are derived from micronuclear precursors in the course of a sexual process termed conjugation. We have found that two apparent micronuclear precursors exist for EFA1. They differ in their potentia...

journal_title：Nucleic acids research

authors： Florian V,Klein A

更新日期：1996-08-15 00:00:00

abstract：:The nature and pace of genome mutation is largely unknown. Because standard methods sequence DNA from populations of cells, the genetic composition of individual cells is lost, de novo mutations in cells are concealed within the bulk signal and per cell cycle mutation rates and mechanisms remain elusive. Although sing...

journal_title：Nucleic acids research

authors： Voet T,Kumar P,Van Loo P,Cooke SL,Marshall J,Lin ML,Zamani Esteki M,Van der Aa N,Mateiu L,McBride DJ,Bignell GR,McLaren S,Teague J,Butler A,Raine K,Stebbings LA,Quail MA,D'Hooghe T,Moreau Y,Futreal PA,Stratton MR

更新日期：2013-07-01 00:00:00

abstract：:Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imin...

journal_title：Nucleic acids research

authors： Kierzek R,He L,Turner DH

更新日期：1992-04-11 00:00:00

abstract：:The last 82 nucleotides of the 6.3 kb genomic RNA of plant turnip yellow mosaic virus (TYMV), the so-called 'tRNA-like' domain, presents functional, structural and primary sequence homologies with canonical tRNAs. In particular, one of the stem-loops resembles the TPsi(pseudouridine)-branch of tRNA, except for the pre...

journal_title：Nucleic acids research

authors： Becker HF,Motorin Y,Florentz C,Giegé R,Grosjean H

更新日期：1998-09-01 00:00:00

abstract：:Plants have large diverse families of small secreted proteins (SSPs) that play critical roles in the processes of development, differentiation, defense, flowering, stress response, symbiosis, etc. Oryza sativa is one of the major crops worldwide and an excellent model for monocotyledonous plants. However, there had no...

journal_title：Nucleic acids research

authors： Pan B,Sheng J,Sun W,Zhao Y,Hao P,Li X

更新日期：2013-01-01 00:00:00

abstract：:The Trypanosoma brucei exoribonuclease, TbDSS-1, has been implicated in multiple aspects of mitochondrial RNA metabolism. Here, we investigate the role of TbDSS-1 in RNA processing and surveillance by analyzing 12S rRNA processing intermediates in TbDSS-1 RNAi cells. RNA fragments corresponding to leader sequence upst...

journal_title：Nucleic acids research

authors： Mattiacio JL,Read LK

更新日期：2008-01-01 00:00:00

abstract：:Increasing numbers of proteins, nucleic acids and other molecular entities have been explored as therapeutic targets, hundreds of which are targets of approved and clinical trial drugs. Knowledge of these targets and corresponding drugs, particularly those in clinical uses and trials, is highly useful for facilitating...

journal_title：Nucleic acids research

authors： Zhu F,Han B,Kumar P,Liu X,Ma X,Wei X,Huang L,Guo Y,Han L,Zheng C,Chen Y

更新日期：2010-01-01 00:00:00

abstract：:A frequently used method of comparing large RNA molecules employs the two-dimensional display of oligonucleotides generated through the action of specific RNases (oligonucleotide mapping, fingerprinting). Using computer simulations and simple analytic expressions the number of large RNase T1-resistant oligonucleotides...

journal_title：Nucleic acids research

authors： Aaronson RP,Young JF,Palese P

更新日期：1982-01-11 00:00:00

abstract：:Coralyne is a small crescent-shaped molecule known to intercalate duplex and triplex DNA. We report that coralyne can cause the complete and irreversible disproportionation of duplex poly(dT)*poly(dA). That is, coralyne causes the strands of duplex poly(dT)*poly(dA) to repartition into equal molar equivalents of tripl...

journal_title：Nucleic acids research

authors： Polak M,Hud NV

更新日期：2002-02-15 00:00:00

abstract：:We have previously described programs for a variety of types of sequence analysis (1-4). These programs have now been integrated into a single package. They are written in the standard C programming language and run on virtually any computer system with a C compiler, such as the IBM/PC and other computers running unde...

journal_title：Nucleic acids research

authors： Mount DW,Conrad B

更新日期：1986-01-10 00:00:00

abstract：:The 338.5 kb of the Escherichia coli genome described here together with previously described segments bring the total of contiguous finished sequence of this genome to > 1 Mb. Of 319 open reading frames (ORFs) found in this 338.5 kb segment, 147 (46%) are potential new genes. The positions of several genes which had ...

journal_title：Nucleic acids research

authors： Burland V,Plunkett G 3rd,Sofia HJ,Daniels DL,Blattner FR

更新日期：1995-06-25 00:00:00

abstract：:Recently published alignments of available 5 S rRNA sequences have shown that a rigid base pairing pattern, pointing to the existence of a universal five-helix secondary structure for all 5 S RNAs, can be superimposed on such alignments. For a few species, the alignment and the base pairing pattern show distortions wi...

journal_title：Nucleic acids research

authors： Dams E,Vandenberghe A,De Wachter R

更新日期：1983-03-11 00:00:00

abstract：:DNA intercalators are found to recognize a DNA lesion as a high affinity receptor site. This lesion-specific binding is observed when one strand of a DNA double helix contains an extra, unpaired nucleotide. Our assay for binding controls for the effects of sequence with a series of oligodeoxynucleotide duplexes which ...

journal_title：Nucleic acids research

authors： Williams LD,Thivierge J,Goldberg IH

更新日期：1988-12-23 00:00:00

abstract：:G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. T...

journal_title：Nucleic acids research

authors： Lu Q,Schierer T,Kang SG,Henderson E

更新日期：1998-04-01 00:00:00

abstract：:The transcription factor Sox5 has previously been shown in chicken to be expressed in early neural crest cells and neural crest-derived peripheral glia. Here, we show in mouse that Sox5 expression also continues after neural crest specification in the melanocyte lineage. Despite its continued expression, Sox5 has litt...

journal_title：Nucleic acids research

authors： Stolt CC,Lommes P,Hillgärtner S,Wegner M

更新日期：2008-10-01 00:00:00

abstract：:Numerous RNA-binding proteins have modular structures, comprising one or several copies of a selective RNA-binding domain generally coupled to an auxiliary domain that binds RNA non-specifically. We have built and compared homology-based models of the cold-shock domain (CSD) of the Xenopus protein, FRGY2, and of the t...

journal_title：Nucleic acids research

authors： Manival X,Ghisolfi-Nieto L,Joseph G,Bouvet P,Erard M

更新日期：2001-06-01 00:00:00

abstract：:Primer extension studies have shown that the Y-family DNA polymerase IV (Dpo4) from Sulfolobus solfataricus P2 can preferentially insert C opposite N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (AAF-dG) [F. Boudsocq, S. Iwai, F. Hanaoka and R. Woodgate (2001) Nucleic Acids Res., 29, 4607-4616]. Our goal is to elucidate...

journal_title：Nucleic acids research

authors： Wang L,Broyde S

更新日期：2006-02-01 00:00:00

abstract：:Chromosomes in vertebrates are protected at both ends by telomere DNA composed of tandem (TTAGGG)n repeats. DNA replication produces a blunt-ended leading strand telomere and a lagging strand telomere carrying a single-stranded G-rich overhang at its end. The G-rich strand can form G-quadruplex structure in the presen...

journal_title：Nucleic acids research

authors： Kan ZY,Lin Y,Wang F,Zhuang XY,Zhao Y,Pang DW,Hao YH,Tan Z

更新日期：2007-01-01 00:00:00

abstract：:The FALC-Loop web server provides an online interface for protein loop modeling by employing an ab initio loop modeling method called FALC (fragment assembly and analytical loop closure). The server may be used to construct loop regions in homology modeling, to refine unreliable loop regions in experimental structures...

journal_title：Nucleic acids research

authors： Ko J,Lee D,Park H,Coutsias EA,Lee J,Seok C

更新日期：2011-07-01 00:00:00

abstract：:We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as templates, where b...

journal_title：Nucleic acids research

authors： Kojima T,Takei Y,Ohtsuka M,Kawarasaki Y,Yamane T,Nakano H

更新日期：2005-10-06 00:00:00

abstract：:Applications of conditional gene expression, whether for therapeutic or basic research purposes, are increasingly requiring mammalian gene control systems that exhibit far tighter control properties. While numerous approaches have been used to improve the widely used Tet-regulatory system, many applications, particula...

journal_title：Nucleic acids research

authors： Greber D,El-Baba MD,Fussenegger M

更新日期：2008-09-01 00:00:00