Abstract：
:G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. TGP1 was purified by three-column chromatographies, including a G-DNA affinity column. Two major proteins (approximately 80 and approximately 40 kDa) were present in the most highly purified column fraction. Renaturation experiments showed that the approximately 80 kDa protein contains TGP1 activity. Biochemical characterization showed that TGP1 is a G-DNA specific binding protein with a preference for parallel G-DNAs. The TGP1/DNA complex has a dissociation constant (Kd) of approximately 2.2 x 10(-8) M and TGP1 can form supershift in gel mobility shift assays. The cDNA coding TGP1 was cloned and sequenced based upon an internal peptide sequence obtained from the approximately 80 kDa protein. Sequence analyses showed that TGP1 is a basic protein with a pI of 10.58, and contains two extensively hydrophilic and basic domains. Homology searches revealed that TGP1 is a novel protein sharing weak similarities with a number of proteins.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Lu Q,Schierer T,Kang SG,Henderson Edoi
10.1093/nar/26.7.1613subject
Has Abstractpub_date
1998-04-01 00:00:00pages
1613-20issue
7eissn
0305-1048issn
1362-4962pii
gkb313journal_volume
26pub_type
杂志文章abstract：:Topoisomerase II, purified from chicken erythrocytes, was reacted with a large number of different DNA fragments and cleavages were catalogued in the presence and absence of drugs that stabilize the cleavage intermediate. Cleavages were sequenced to derive a consensus for topoisomerase II that predicts catalytic sites...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.12.5533
更新日期：1988-06-24 00:00:00
abstract：:Aberration within the p53 tumor suppressor gene is the most frequently identified genetic damage in human cancer. Regulatory functions proposed for the p53 protein include modulation of the cell cycle, cellular differentiation, signal transduction, and gene expression. Additionally, the p53 gene product may guard the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1993-02-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv514
更新日期：2015-07-13 00:00:00
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更新日期：1985-04-25 00:00:00
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更新日期：2017-04-07 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2008-12-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2017-01-09 00:00:00
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journal_title：Nucleic acids research
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更新日期：2018-07-06 00:00:00
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更新日期：2009-11-01 00:00:00
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更新日期：1996-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：1987-01-12 00:00:00
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更新日期：2001-01-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/7.2.493
更新日期：1979-09-25 00:00:00
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pub_type： 杂志文章
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更新日期：1984-01-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：1991-08-11 00:00:00
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更新日期：2013-02-01 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2012-03-01 00:00:00
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更新日期：2017-11-02 00:00:00
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journal_title：Nucleic acids research
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更新日期：2009-04-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：1996-08-15 00:00:00
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更新日期：2004-12-01 00:00:00
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journal_title：Nucleic acids research
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更新日期：2009-08-01 00:00:00
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更新日期：2006-07-01 00:00:00
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更新日期：2003-06-15 00:00:00
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更新日期：2015-04-30 00:00:00
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更新日期：1986-09-11 00:00:00
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更新日期：1991-07-11 00:00:00
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journal_title：Nucleic acids research
pub_type： 杂志文章
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更新日期：2012-07-01 00:00:00
