Abstract:
:Allosteric nucleic acid ligases have been used previously to transform analyte-binding into the formation of oligonucleotide templates that can be amplified and detected. We have engineered binary deoxyribozyme ligases whose two components are brought together by bridging oligonucleotide effectors. The engineered ligases can 'read' one sequence and then 'write' (by ligation) a separate, distinct sequence, which can in turn be uniquely amplified. The binary deoxyribozymes show great specificity, can discriminate against a small number of mutations in the effector, and can read and recode DNA information with high fidelity even in the presence of excess obscuring genomic DNA. In addition, the binary deoxyribozymes can read non-natural nucleotides and write natural sequence information. The binary deoxyribozyme ligases could potentially be used in a variety of applications, including the detection of single nucleotide polymorphisms in genomic DNA or the identification of short nucleic acids such as microRNAs.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Tabor JJ,Levy M,Ellington ADdoi
10.1093/nar/gkl176subject
Has Abstractpub_date
2006-04-28 00:00:00pages
2166-72issue
8eissn
0305-1048issn
1362-4962pii
34/8/2166journal_volume
34pub_type
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