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Processing of telomeric DNA ends requires the passage of a replication fork.

Abstract：

:During telomere replication in yeast, chromosome ends acquire a long single-stranded extension of the strand making the 3' end. Previous work showed that these 3' tails are generated late in S-phase, when conventional replication is virtually complete. In addition, the extensions were also observed in cells that lacked telomerase. Therefore, a model was proposed that predicted an activity that recessed the 5' ends at yeast telomeres after conventional replication was complete. Here, we demonstrate that this processing activity is dependent on the passage of a replication fork through yeast telomeres. A non-replicating linear plasmid with telomeres at each end does not acquire single-stranded extensions, while an identical construct containing an origin of replication does. Thus, the processing activity could be associated with the enzymes at the replication fork itself, or the passage of the fork through the telomeric sequences allows a transient access for the activity to the telomeres. We therefore propose that there is a mechanistic link between the conventional replication machinery and telomere maintenance.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Dionne I,Wellinger RJ

doi

10.1093/nar/26.23.5365

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

5365-71

issue

23

eissn

0305-1048

issn

1362-4962

pii

gkb861

journal_volume

26

pub_type

杂志文章

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