A non-canonical multisubunit RNA polymerase encoded by a giant bacteriophage.


:The infection of Pseudomonas aeruginosa by the giant bacteriophage phiKZ is resistant to host RNA polymerase (RNAP) inhibitor rifampicin. phiKZ encodes two sets of polypeptides that are distantly related to fragments of the two largest subunits of cellular multisubunit RNAPs. Polypeptides of one set are encoded by middle phage genes and are found in the phiKZ virions. Polypeptides of the second set are encoded by early phage genes and are absent from virions. Here, we report isolation of a five-subunit RNAP from phiKZ-infected cells. Four subunits of this enzyme are cellular RNAP subunits homologs of the non-virion set; the fifth subunit is a protein of unknown function. In vitro, this complex initiates transcription from late phiKZ promoters in rifampicin-resistant manner. Thus, this enzyme is a non-virion phiKZ RNAP responsible for transcription of late phage genes. The phiKZ RNAP lacks identifiable assembly and promoter specificity subunits/factors characteristic for eukaryal, archaeal and bacterial RNAPs and thus provides a unique model for comparative analysis of the mechanism, regulation and evolution of this important class of enzymes.


Nucleic Acids Res


Nucleic acids research


Yakunina M,Artamonova T,Borukhov S,Makarova KS,Severinov K,Minakhin L




Has Abstract


2015-12-02 00:00:00














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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sirdeshmukh R,Schlessinger D

    更新日期:1985-07-25 00:00:00

  • Increased G + C content of DNA stabilizes methyl CpG dinucleotides.

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    journal_title:Nucleic acids research

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    authors: Adams RL,Eason R

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  • Point mutations in the Rpb9-homologous domain of Rpc11 that impair transcription termination by RNA polymerase III.

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    journal_title:Nucleic acids research

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    authors: Iben JR,Mazeika JK,Hasson S,Rijal K,Arimbasseri AG,Russo AN,Maraia RJ

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    authors: Qin J,Fauron CM,Brettell RI,Milhous M,Abbott AG

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    journal_title:Nucleic acids research

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    authors: Fickett JW

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    journal_title:Nucleic acids research

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    authors: Wevrick R,Willard HF

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    journal_title:Nucleic acids research

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    authors: Wiebers JL

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    journal_title:Nucleic acids research

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    authors: Hara Y,Onishi Y,Oishi K,Miyazaki K,Fukamizu A,Ishida N

    更新日期:2009-03-01 00:00:00

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    journal_title:Nucleic acids research

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    authors: Llinás M,Bozdech Z,Wong ED,Adai AT,DeRisi JL

    更新日期:2006-02-21 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Foissac S,Sammeth M

    更新日期:2007-07-01 00:00:00

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    journal_title:Nucleic acids research

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    authors: Xu Y,Guo M,Liu X,Wang C,Liu Y,Liu G

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    authors: Rosu F,Gabelica V,Houssier C,De Pauw E

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    authors: Hanes JW,Johnson KA

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    authors: Wang TW,Lewin AS,Small GM

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  • Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions.

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    authors: Aoyama M,Agari K,Sun-Wada GH,Futai M,Wada Y

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    journal_title:Nucleic acids research

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    authors: Saunders CA,Bostian KA,Halvorson HO

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    journal_title:Nucleic acids research

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    authors: Xiong Z,Laird PW

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Griffiths-Jones S,Moxon S,Marshall M,Khanna A,Eddy SR,Bateman A

    更新日期:2005-01-01 00:00:00

  • The abundance of RNPS1, a protein component of the exon junction complex, can determine the variability in efficiency of the Nonsense Mediated Decay pathway.

    abstract::Nonsense-mediated mRNA decay (NMD) is a molecular pathway of mRNA surveillance that ensures rapid degradation of mRNAs containing premature translation termination codons (PTCs) in eukaryotes. NMD has been shown to also regulate normal gene expression and thus emerged as one of the key post-transcriptional mechanisms ...

    journal_title:Nucleic acids research

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    authors: Viegas MH,Gehring NH,Breit S,Hentze MW,Kulozik AE

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    authors: Fujimoto K,Iida H,Kawakami M,Bando T,Tao ZF,Sugiyama H

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    authors: Pei DS,Yang XJ,Liu W,Guikema JE,Schrader CE,Strauss PR

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    pub_type: 杂志文章


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    更新日期:2016-04-20 00:00:00

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Dower WJ,Miller JF,Ragsdale CW

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    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Jobbins AM,Reichenbach LF,Lucas CM,Hudson AJ,Burley GA,Eperon IC

    更新日期:2018-03-16 00:00:00