Abstract:
:DNA polymerase gamma from purified nuclei of EMT-6 cells (mice) seems to be identical to the mitochondrial DNA polymerase from the same source following several criteria. These two enzyme activities are strongly inhibited by ethidium bromide and acriflavin, while proflavin, acridine orange, daunomycin and chloroquine inhibition is less pronounced. In the case of DNA polymerases alpha and beta very little inhibition by ethidium bromide was observed. Intercalation of this dye in a poly dA-dT 12-18 template-primer was studied spectrophotometrically under conditions similar to those in the in vitro DNA polymerase assay. The polymerase assay. The inhibition by this drug of the mitochondrial DNA polymerase gamma activity was shown to be competitive at varying concentrations of TTP while the inhibition was of the non-competitive type at different concentrations of poly dA-dT 12-18. We conclude that the drug, most probably in the intercalated form, is able to interact with the active site (s) of mitochondrial DNA polymerase.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Tarrago-Litvak L,Viratelle O,Darriet D,Dalibart R,Graves PV,Litvak Sdoi
10.1093/nar/5.6.2197subject
Has Abstractpub_date
1978-06-01 00:00:00pages
2197-210issue
6eissn
0305-1048issn
1362-4962journal_volume
5pub_type
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