Abstract:
:Among four types of bacterial restriction enzymes that cleave a foreign DNA depending on its methylation status, type I enzymes composed of three subunits are interesting because of their unique DNA cleavage and translocation mechanisms performed by the restriction subunit (HsdR). The elucidated N-terminal fragment structure of a putative HsdR subunit from Vibrio vulnificus YJ016 reveals three globular domains. The nucleolytic core within an N-terminal nuclease domain (NTD) is composed of one basic and three acidic residues, which include a metal-binding site. An ATP hydrolase (ATPase) site at the interface of two RecA-like domains (RDs) is located close to the probable DNA-binding site for translocation, which is far from the NTD nucleolytic core. Comparison of relative domain arrangements with other functionally related ATP and/or DNA complex structures suggests a possible translocation and restriction mechanism of the HsdR subunit. Furthermore, careful analysis of its sequence and structure implies that a linker helix connecting two RDs and an extended region within the nuclease domain may play a central role in switching the DNA translocation into the restriction activity.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Uyen NT,Park SY,Choi JW,Lee HJ,Nishi K,Kim JSdoi
10.1093/nar/gkp603subject
Has Abstractpub_date
2009-11-01 00:00:00pages
6960-9issue
20eissn
0305-1048issn
1362-4962pii
gkp603journal_volume
37pub_type
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