Molecular analysis of RNA polymerase alpha subunit gene from Streptomyces coelicolor A3(2).

abstract：:Within a single infected individual, a virus population can have a high genomic variability. In the case of HIV, several mutations can be present even in a small genomic window of 20-30 nucleotides. For diagnostics purposes, it is often needed to resequence genomic subsets where crucial mutations are known to occur. I...

journal_title：Nucleic acids research

authors： Hadiwikarta WW,Van Dorst B,Hollanders K,Stuyver L,Carlon E,Hooyberghs J

更新日期：2013-10-01 00:00:00

abstract：:The DNA-binding protein RdgC has been identified as an inhibitor of RecA-mediated homologous recombination in Escherichia coli. In Neisseria species, RdgC also has a role in virulence-associated antigenic variation. We have previously solved the crystal structure of the E. coli RdgC protein and shown it to form a toro...

journal_title：Nucleic acids research

authors： Briggs GS,Yu J,Mahdi AA,Lloyd RG

更新日期：2010-10-01 00:00:00

abstract：:The chromatin structure of yeast ribosomal DNA was analyzed in vivo by crosslinking intact cells with psoralen. We found that in exponentially growing cultures the regions coding for the 35S rRNA precursor fall into two distinct classes. One class was highly accessible to psoralen and associated with nascent RNAs, cha...

journal_title：Nucleic acids research

authors： Dammann R,Lucchini R,Koller T,Sogo JM

更新日期：1993-05-25 00:00:00

abstract：:Increased cytochrome P450 2E1 (CYP2E1) expression is the main cause of oxidative stress, which exacerbates alcoholic liver diseases (ALDs). Estrogen-related receptor gamma (ERRγ) induces CYP2E1 expression and contributes to enhancing alcohol-induced liver injury. Retinoic acid-related orphan receptor alpha (RORα) has ...

journal_title：Nucleic acids research

authors： Han YH,Kim DK,Na TY,Ka NL,Choi HS,Lee MO

更新日期：2016-02-18 00:00:00

abstract：:A new class of synthetic DNA duplexes containing repeating oligonucleotide sequences, double-helical concatemers, is characterized. The UV-absorption and circular dichroism of a concatemer formed in self-association of d(T-G-C-A-C-A-T-G) have been studied. The thermodynamical parameters of complex formation are the fo...

journal_title：Nucleic acids research

authors： Shabarova ZA,Dolinnaya NG,Turkin SI,Gromova ES

更新日期：1980-06-11 00:00:00

abstract：:Mitochondrial DNA from human tissue culture cells contains heterogeneous sequences located within a previously identified, evolutionarily conserved region termed CSB-2. CSB-2 is located near the origin of heavy-strand mitochondrial DNA synthesis and the major transcriptional promoters for each strand of human mitochon...

journal_title：Nucleic acids research

authors： Hauswirth WW,Clayton DA

更新日期：1985-11-25 00:00:00

abstract：:Reactive oxygen species (ROS) have been implicated as the cause of cumulative damage to DNA, proteins and lipids that can ultimately result in cell death. A common problem when measuring oxidative DNA damage has been the introduction of modifications in the native state of the molecule by many DNA isolation methods. W...

journal_title：Nucleic acids research

authors： Milano J,Day BJ

更新日期：2000-02-15 00:00:00

abstract：:Initiation of bacterial DNA replication at oriC is mediated by primosomal proteins that act cooperatively to melt an AT-rich region where the replicative helicase is loaded prior to the assembly of the replication fork. In Bacillus subtilis, the dnaD, dnaB and dnaI genes are essential for initiation of DNA replication...

journal_title：Nucleic acids research

authors： Grainger WH,Machón C,Scott DJ,Soultanas P

更新日期：2010-05-01 00:00:00

abstract：:During translation initiation, eukaryotic initiation factor 2 (eIF2) delivers the Met-tRNA to the 40S ribosomal subunit to locate the initiation codon (AUGi) of mRNA during the scanning process. Stress-induced eIF2 phosphorylation leads to a general blockade of translation initiation and represents a key antiviral pat...

journal_title：Nucleic acids research

authors： Toribio R,Díaz-López I,Boskovic J,Ventoso I

更新日期：2016-05-19 00:00:00

abstract：:An investigation of the mobility of short duplexes of DNA -octamers and decamers-on polyacrylamide gels is presented, showing that molecules containing less than one helical turn exhibit sequence dependent mobilities. Analysis of chains with different sequences indicates that any arrangement of two or more adjacent A....

journal_title：Nucleic acids research

authors： Chen JH,Seeman NC,Kallenbach NR

更新日期：1988-07-25 00:00:00

abstract：:Homing endonuclease genes (HEGs) are mobile DNA elements that are thought to confer no benefit to their host. They encode site-specific DNA endonucleases that perpetuate the element within a species population by homing and disseminate it between species by horizontal transfer. Several yeast species contain the VMA1 H...

journal_title：Nucleic acids research

authors： Posey KL,Koufopanou V,Burt A,Gimble FS

更新日期：2004-07-27 00:00:00

abstract：:The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters face each other and overlap with an operator region containing two domains which may act as distinct repressor binding si...

journal_title：Nucleic acids research

authors： Piette J,Cunin R,Boyen A,Charlier D,Crabeel M,Van Vliet F,Glansdorff N,Squires C,Squires CL

更新日期：1982-12-20 00:00:00

abstract：:This paper describes a computer program designed to look for similarities between pairs of nucleic or amino acid sequences. The program looks both for segments of perfect identity or for regions where, using a scoring matrix, a minimum value is exceeded. The results of comparisons are presented as a matrix which is di...

journal_title：Nucleic acids research

authors： Staden R

更新日期：1982-05-11 00:00:00

abstract：:The human RAD54B protein is a paralog of the RAD54 protein, which plays important roles in homologous recombination. RAD54B contains an N-terminal region outside the SWI2/SNF2 domain that shares less conservation with the corresponding region in RAD54. The biochemical roles of this region of RAD54B are not known, alth...

journal_title：Nucleic acids research

authors： Sarai N,Kagawa W,Fujikawa N,Saito K,Hikiba J,Tanaka K,Miyagawa K,Kurumizaka H,Yokoyama S

更新日期：2008-10-01 00:00:00

abstract：:An efficient procedure for the synthesis of unnatural alpha-anomeric oligodeoxyribonucleotides is described. This solid-phase procedure is based on the use of alpha-nucleoside phosphoramidites and alpha-nucleoside derivatized solid supports corresponding to the four natural bases and allow rapid synthesis of oligonucl...

journal_title：Nucleic acids research

authors： Morvan F,Rayner B,Leonetti JP,Imbach JL

更新日期：1988-02-11 00:00:00

abstract：:The polymerase encoded by human hepatitis B virus, which has reverse transcriptase and RNase H activity, binds to its pregenomic RNA template in a two-step process involving a terminal redundancy. Both first strand and second strand DNA synthesis involve primer translocation and second strand synthesis involves a temp...

journal_title：Nucleic acids research

authors： Kidd AH,Kidd-Ljunggren K

更新日期：1996-09-01 00:00:00

abstract：:Previously we identified Rrp1 and Rrp2 as two proteins required for the Sfr1/Swi5-dependent branch of homologous recombination (HR) in Schizosaccharomyces pombe. Here we use a yeast two-hybrid approach to demonstrate that Rrp1 and Rrp2 can interact with each other and with Swi5, an HR mediator protein. Rrp1 and Rrp2 f...

journal_title：Nucleic acids research

authors： Dziadkowiec D,Kramarz K,Kanik K,Wisniewski P,Carr AM

更新日期：2013-09-01 00:00:00

abstract：:Vascular endothelial growth factor A (VEGF-A) is a master regulator of angiogenesis, vascular development and function. In this study we investigated the transcriptional regulation of VEGF-A-responsive genes in primary human aortic endothelial cells (HAECs) and human umbilical vein endothelial cells (HUVECs) using gen...

journal_title：Nucleic acids research

authors： Kaikkonen MU,Niskanen H,Romanoski CE,Kansanen E,Kivelä AM,Laitalainen J,Heinz S,Benner C,Glass CK,Ylä-Herttuala S

更新日期：2014-11-10 00:00:00

abstract：:The mouse is widely used as system to study human genetic mechanisms. However, extensive rewiring of transcriptional regulatory networks often confounds translation of findings between human and mouse. Site-specific gain and loss of individual transcription factor binding sites (TFBS) has caused functional divergence ...

journal_title：Nucleic acids research

authors： Diehl AG,Boyle AP

更新日期：2018-02-28 00:00:00

abstract：:In a gene targeting experiment, the generation of a targeting construct often requires complex DNA manipulations. We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dual prokaryotic and e...

journal_title：Nucleic acids research

authors： Aoyama M,Agari K,Sun-Wada GH,Futai M,Wada Y

更新日期：2005-03-22 00:00:00

abstract：:TGGCA-binding proteins are nuclear proteins with high affinity for double-stranded DNA homologous to the prototype recognition sequence 5'YTGGCANNNTGCCAR 3'. Their ubiquitous tissue distribution in higher vertebrates characterizes them as a class of highly conserved proteins which may exert a basic function. To obtain...

journal_title：Nucleic acids research

authors： Nowock J,Borgmeyer U,Püschel AW,Rupp RA,Sippel AE

更新日期：1985-03-25 00:00:00

abstract：:Orthology is a widely used concept in comparative and evolutionary genomics. In addition to prokaryotic orthology, delineating eukaryotic orthology has provided insight into the evolution of higher organisms. Indeed, many eukaryotic ortholog databases have been established for this purpose. However, unlike prokaryotes...

journal_title：Nucleic acids research

authors： Ho MR,Jang WJ,Chen CH,Ch'ang LY,Lin WC

更新日期：2008-06-01 00:00:00

abstract：:We have studied the effect of temperature on transcription initiation in vitro at two related promoters ga/Pcon and ga/P1, which have the same nucleotide sequence around the -10 region and transcription start site, but differ in upstream sequences. One of the promoters, ga/Pcon, carries the consensus -35 hexamer, 5'TT...

journal_title：Nucleic acids research

authors： Grimes E,Busby S,Minchin S

更新日期：1991-11-25 00:00:00

abstract：:7SK snRNA, an abundant RNA discovered in human nucleus, regulates transcription by RNA polymerase II (RNAPII). It sequesters and inhibits the transcription elongation factor P-TEFb which, by phosphorylation of RNAPII, switches transcription from initiation to processive elongation and relieves pauses of transcription....

journal_title：Nucleic acids research

authors： Lebars I,Martinez-Zapien D,Durand A,Coutant J,Kieffer B,Dock-Bregeon AC

更新日期：2010-11-01 00:00:00

abstract：:IGF2 mRNA-binding protein 1 (IMP1) is a key regulator of messenger RNA (mRNA) metabolism and transport in organismal development and, in cancer, its mis-regulation is an important component of tumour metastasis. IMP1 function relies on the recognition of a diverse set of mRNA targets that is mediated by the combinator...

journal_title：Nucleic acids research

authors： Dagil R,Ball NJ,Ogrodowicz RW,Hobor F,Purkiss AG,Kelly G,Martin SR,Taylor IA,Ramos A

更新日期：2019-05-07 00:00:00

abstract：:The nucleotide excision repair pathway removes ultraviolet (UV) photoproducts from the human genome in the form of short oligonucleotides ∼ 30 nt in length. Because there are limitations to many of the currently available methods for investigating UV photoproduct repair in vivo, we developed a convenient non-radioisot...

journal_title：Nucleic acids research

authors： Choi JH,Gaddameedhi S,Kim SY,Hu J,Kemp MG,Sancar A

更新日期：2014-02-01 00:00:00

abstract：:The 4.5S rRNA was isolated from the chloroplast ribosomes from Dryopteris acuminata. The complete nucleotide sequence was determined to be: OHUAAGGUCACGGCAAGACGAGCCGUUUAUCACCACGAUAGGUGCUAAGUGGAGGUGCAGUAAUGUAUGCAGCUGAGGC AUCCUAAUAGACCGAGAGGUUUGAACOH. The 4.5S rRNA is composed of 103 nucleotides and shows strong homolog...

journal_title：Nucleic acids research

authors： Takaiwa F,Kusuda M,Sugiura M

更新日期：1982-04-10 00:00:00

abstract：:NusG is an essential transcription factor that plays multiple key regulatory roles in transcription elongation, termination and coupling translation and transcription. The core role of NusG is to enhance transcription elongation and RNA polymerase processivity. Here, we present the structure of Escherichia coli RNA po...

journal_title：Nucleic acids research

authors： Liu B,Steitz TA

更新日期：2017-01-25 00:00:00

abstract：:Two new beta-lactoglobulin (BLG)/human serum albumin (HSA) hybrid gene vectors were constructed and tested for expression in COS-7 cells and in transgenic mice. The HSA sequences were inserted between the second and sixth BLG exons. Transient transfection experiments with these vectors as well as a series of additiona...

journal_title：Nucleic acids research

authors： Barash I,Nathan M,Kari R,Ilan N,Shani M,Hurwitz DR

更新日期：1996-02-15 00:00:00

abstract：:Some human cancers maintain their telomeres using the alternative lengthening of telomeres (ALT) mechanism; a process thought to involve recombination. Different types of recombinational telomere elongation pathways have been identified in yeasts. In senescing yeast telomerase deletion (ter1-Delta) mutants with very s...

journal_title：Nucleic acids research

authors： Basenko EY,Cesare AJ,Iyer S,Griffith JD,McEachern MJ

更新日期：2010-01-01 00:00:00