Abstract：
:Resonances of the water exchangeable iminoprotons of the acceptor and D stem of yeast tRNAPhe have been assigned by means of Nuclear Overhauser Effects (NOE's). Assignments were made for spectra recorded from tRNA dialysed against a buffer with 110 mM sodium and 5 mM magnesium ions and against a buffer with 430 mM sodium and no magnesium ions. Remarkable is the assignment of a resonance at 13.6 - 13.7 ppm to the iminoproton of C11G24. This assignment as well as those of G1C72, G3C70, U7A66, U12A23 and C13G22 are different from those made previously on the basis of less direct evidence. NOE experiments performed at 45 degrees C support the view that the D stem together with the tertiary interaction U8A14 is one of the most stable parts of the molecule in the presence of magnesium ions. A comparison of the spectra recorded under the two different buffer conditions shows that an excess of 320 mM sodium ions is not capable to force the tRNA in the same conformation as 5 mM magnesium ions can do.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Heerschap A,Haasnoot CA,Hilbers CWdoi
10.1093/nar/10.21.6981subject
Has Abstractpub_date
1982-11-11 00:00:00pages
6981-7000issue
21eissn
0305-1048issn
1362-4962journal_volume
10pub_type
杂志文章abstract：:We have isolated a second gene (MLS1), which in addition to DAL7, encodes malate synthase from S. cerevisiae. Expression of the two genes is specific for their physiological roles in carbon and nitrogen metabolism. Expression of MLS1, which participates in the utilization of non-fermentable carbon sources, is sensitiv...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.21.5677
更新日期：1992-11-11 00:00:00
abstract：:The human telomeric DNA sequence with four repeats can fold into a parallel-stranded propeller-type topology. NMR structures solved under molecular crowding experiments correlate with the crystal structures found with crystal-packing interactions that are effectively equivalent to molecular crowding. This topology has...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks1331
更新日期：2013-02-01 00:00:00
abstract：:A library of three synthetic ribozymes with randomized arms, targeting NUX, GUX and NXG triplets, respectively, were used to identify ribozyme-accessible sites on the HIV-1 LTR transcript comprising positions -533 to 386. Three cleavable sites were identified at positions 109, 115 and 161. Ribozymes were designed agai...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.21.4059
更新日期：2000-11-01 00:00:00
abstract：:G-DNA, a polymorphic family of four-stranded DNA structures, has been proposed to play roles in a variety of biological processes including telomere function, meiotic recombination and gene regulation. Here we report the purification and cloning of TGP1, a G-DNA specific binding protein from Tetrahymena thermophila. T...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.7.1613
更新日期：1998-04-01 00:00:00
abstract：:RNA molecules of > 20 nucleotides have been the focus of numerous recent NMR structural studies. Several investigators have used the UNCG family of hairpins to ensure proper folding. We show that th UUCG hairpin has a minimum requirement of a two base-pair stem. Hairpins with a CG loop closing base pair and an initial...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.15.3056
更新日期：1995-08-11 00:00:00
abstract：:The design and synthesis of metal complexes that can specifically target DNA secondary structure has attracted considerable attention. Chiral metallosupramolecular complexes (e.g. helicates) in particular display unique DNA-binding behavior, however until recently few examples which are both water-compatible and enant...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx244
更新日期：2017-05-19 00:00:00
abstract：:The S-adenosylmethionine (AdoMet) analog sinefungin is a natural product antibiotic that inhibits nucleic acid methyltransferases and arrests the growth of unicellular eukarya and eukaryal viruses. The basis for the particular sensitivity of fungi and protozoa to sinefungin is not known. Here we report the isolation a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm817
更新日期：2007-01-01 00:00:00
abstract：:Rice alpha-amylases are encoded by a multigene family that has previously been classified into 5 hybridization groups. DNA sequence and Southern blot analysis identified three genes (RAmy1A, RAmy1B and RAmy1C) in Group 1 with DNA sequence identity of at least 90%. Hybridization Group 2 is represented by only one gene,...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.23.7007
更新日期：1990-12-11 00:00:00
abstract：:We characterized and purified a protein from rat liver which specifically binds to a DNA motif present in a liver-specific enhancer of the rat tyrosine aminotransferase (TAT) gene, when offered as single-stranded DNA. Binding is highly sequence-specific and coincides with a region known to be essential for function of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.6.1243
更新日期：1992-03-25 00:00:00
abstract：:A cDNA that encodes a transcription factor able to recognize the thyroglobulin gene promoter in vitro was isolated from a dog thyroid cDNA expression library in lambda gt11. The library was screened with a multimerized 20 bp-oligonucleotide probe corresponding to the -126 to -107 bp region of the bovine thyroglobulin ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.5.1121
更新日期：1991-03-11 00:00:00
abstract：:Human thymine-DNA glycosylase (TDG) is well known to excise thymine and uracil from G.T and G.U mismatches, respectively, and was therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine. In this study, we characterized ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg344
更新日期：2003-05-01 00:00:00
abstract：:Ribosomal protein L11 and its associated binding site on 23S rRNA together comprise one of the principle components that mediate interactions of translation factors with the ribosome. This site is also the target of the antibiotic thiostrepton, which has been proposed to act by preventing important structural transiti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkh644
更新日期：2004-06-15 00:00:00
abstract：:Intestinal apolipoprotein B mRNA is edited at nucleotide 6666 by a C to U transition resulting in a translational stop codon. The enzymatic properties of the editing activity were characterised in vitro using rat enterocyte cytosolic extract. The editing activity has no nucleotide or ion cofactor requirement. It shows...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/19.13.3569
更新日期：1991-07-11 00:00:00
abstract：:DNA helicases are required for virtually every aspect of DNA metabolism, including replication, repair, recombination and transcription. A comprehensive description of these essential biochemical processes requires detailed understanding of helicase mechanisms. These enzymes are ubiquitous, having been identified in v...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkl501
更新日期：2006-01-01 00:00:00
abstract：:Fourteen P-element insertion mutants at the yellow (y) gene of D. melanogaster have been analysed by restriction mapping and DNA sequencing. The distribution of the insertion sites is nonrandom, with a preferred target site in the 5'-transcribed but non-translated leader of the gene. Transcription analysis indicates t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.7.3039
更新日期：1988-04-11 00:00:00
abstract：:Two types of different repetitive DNA sequences called RepMP1 and RepMP2 were identified in the genome of Mycoplasma pneumoniae. The number of these repeated elements, their nucleotide sequence and their localization on a physical map of the M. pneumoniae genome were determined. The results show that RepMP1 appears at...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.17.8337
更新日期：1988-09-12 00:00:00
abstract：:A formaldehyde denaturation map of the replicative form of phiX174 DNA is obtained. The RFI DNA was converted into a linear state by restriction endonuclease pst I which introduces into this DNA a single double-stranded break. The map has four clear-cut peaks. Their positions excellently correlate with the peak positi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/5.7.2493
更新日期：1978-07-01 00:00:00
abstract：:For the rapid and sensitive detection of p53 'hot spot' mutations, we combined polymerase chain reaction based single-strand conformational polymorphism (PCR-SSCP) analysis with sequence specific-clamping by peptide nucleic acids (PNAs) in a one-step reaction tube protocol. For this purpose, we designed two PNA molecu...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.5.1356
更新日期：1998-03-01 00:00:00
abstract：:The multifunctional RNA-dependent RNA polymerase L protein of vesicular stomatitis virus catalyzes unconventional pre-mRNA capping via the covalent enzyme-pRNA intermediate formation, which requires the histidine-arginine (HR) motif in the polyribonucleotidyltransferase domain. Here, the effects of cap-defective mutat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku901
更新日期：2014-10-29 00:00:00
abstract：:The database of 3D interacting domains (3did, available online for browsing and bulk download at http://3did.irbbarcelona.org) is a catalog of protein-protein interactions for which a high-resolution 3D structure is known. 3did collects and classifies all structural templates of domain-domain interactions in the Prote...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt887
更新日期：2014-01-01 00:00:00
abstract：:The effect of UV-irradiation on the conversion of the single-stranded DNA of the parvovirus Minute-Virus-of-Mice (MVM) to duplex Replicative Forms (RF) was studied after infection of mouse A9 fibroblasts. UV-irradiation of the virus prior to infection of unirradiated cells resulted in a dose-dependent, single-hit, inh...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.8.2577
更新日期：1982-04-24 00:00:00
abstract：:Segments of SV40 DNA having homologous overlapping termini recombine to produce viable genomes in monkey cells. Frequencies of recombination on either side of a deletion marker are non-random; replication and palindromes do not appear to be essential. Since recombination involves host enzymes, a suitable system has be...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/8.12.2725
更新日期：1980-06-25 00:00:00
abstract：:Gene expression is a multi-step process involving many layers of regulation. The main regulators of the pathway are DNA and RNA binding proteins. While over the years, a large number of DNA and RNA binding proteins have been identified and extensively studied, it is still expected that many other proteins, some with y...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw454
更新日期：2016-07-08 00:00:00
abstract：:We have isolated an eight kilobase fragment of Bacillus subtilis DNA by specific integration and excision of a plasmid containing a sequence adjacent to ribosomal operon rrn O. The genetic locus of the cloned fragment was verified by linkage of the integrated vector to nearby genetic markers using both transduction an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.15.6307
更新日期：1984-08-10 00:00:00
abstract：:PIWI-interacting RNAs (piRNAs) engage PIWI proteins to silence transposons and promote germ cell development in animals. In diverse species, piRNA biogenesis occurs near the mitochondrial surface, and involves mitochondrial membrane-anchored factors. In mice, two cytoplasmic PIWI proteins, MIWI and MILI, receive proce...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1281
更新日期：2019-03-18 00:00:00
abstract：:The use of bacterial artificial chromosomes (BACs) provides a consistent and high targeting efficiency of homologous recombination in embryonic stem (ES) cells, facilitated by long stretches of sequence homology. Here, we introduce a BAC targeting method which employs restriction fragment length polymorphisms (RFLPs) ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr550
更新日期：2011-10-01 00:00:00
abstract：:Using a bio-oligo pull-down DNA-binding assay we investigated the binding capacity of endogenous, DNA damage-induced p53 in human diploid fibroblasts to several p53-responsive elements (REs) present in p53-regulated genes. During the course of p53 accumulation, we observed a decrease in p53 binding to the GADD45 but n...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.11.2340
更新日期：2002-06-01 00:00:00
abstract：:From a sarkosyl-insoluble outer membrane fraction prepared from the Helicobacter pylori strain ATCC 43504, 19 proteins could be sequenced N-terminally by Edman degradation. Oligonucleotides were deduced and used for screening of a genomic library. From the isolated genes, five code for different members of a H.pylori ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.16.3325
更新日期：1999-08-15 00:00:00
abstract：:We present a new coarse grained method for the simulation of duplex DNA. The algorithm uses a generalized multi-harmonic model that can represent any multi-normal distribution of helical parameters, thus avoiding caveats of current mesoscopic models for DNA simulation and representing a breakthrough in the field. The ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa015
更新日期：2020-03-18 00:00:00
abstract：:Single nucleotide polymorphisms (SNPs) are, together with copy number variation, the primary source of variation in the human genome. SNPs are associated with altered response to drug treatment, susceptibility to disease and other phenotypic variation. Furthermore, during genetic screens for disease-associated mutatio...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm979
更新日期：2008-01-01 00:00:00
