Abstract:
:Cells respond to DNA damage by activating both cellular growth arrest and DNA repair processes. In Saccharomyces cerevesiae the RAD9 gene controls DNA damage-mediated cell cycle arrest that is known to allow efficient repair. To ascertain whether RAD9 plays a role in DNA repair per se, the removal of UV-induced photolesions was assessed in synchronized isogenic normal and rad9 cells using the high resolution primer extension technique. The results show that RAD9 is indeed involved in the removal of photolesions from both the transcribed and the non-transcribed strands of the reporter GAL10 gene, in G(1)- as well as G(2)/M-arrested cells. Interestingly, these data also reveal that in both normal and rad9 mutant, the repair strand bias towards the transcribed stand is more pronounced in G(2)/M- than in G(1)-arrested cells. These data indicate that RAD9 coordinate the cellular response to DNA damage by activating both cell cycle checkpoint and excision repair.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Al-Moghrabi NM,Al-Sharif IS,Aboussekhra Adoi
10.1093/nar/29.10.2020keywords:
subject
Has Abstractpub_date
2001-05-15 00:00:00pages
2020-5issue
10eissn
0305-1048issn
1362-4962journal_volume
29pub_type
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