Abstract:
:An improved method was developed for microdissection and cloning of metaphase as well as pachytene chromosomes. The protocol incorporates efficient ligation of chromosomal DNA with linker adaptors, abolishment of microcloning steps and the reduction of micromanipulation. The threshold for amplifying genomic DNA template was in the range of 2-20 femtogram. The amplification products had a size distribution between 200 and 1300 bp (average 500 bp). Using pachytene chromosomes of maize the selectivity for segment-specific libraries can be increased between 10- and 20-fold. The approach described here is being applied to the fine mapping and isolation of genes conveying resistance against plant pathogens.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Ponelies N,Stein N,Weber Gdoi
10.1093/nar/25.17.3555subject
Has Abstractpub_date
1997-09-01 00:00:00pages
3555-7issue
17eissn
0305-1048issn
1362-4962pii
gka560journal_volume
25pub_type
杂志文章abstract::The variation of the proton chemical shifts due to the formation intermolecular hydrogen bonds is computed for a number of complexes which can be formed between the bases of the nucleic acids. The shifts expected for the isolated base pairs, in particular for the G-N1 H, T(or U)-N3H protons and the protons of the amin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/4.1.99
更新日期:1977-01-01 00:00:00
abstract::Gaussian network model (GNM) is a simple yet powerful model for investigating the dynamics of proteins and their complexes. GNM analysis became a broadly used method for assessing the conformational dynamics of biomolecular structures with the development of a user-friendly interface and database, iGNM, in 2005. We pr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1236
更新日期:2016-01-04 00:00:00
abstract::Posttranslational modifications (PTMs) of histones represent a crucial regulatory mechanism of nucleosome and chromatin dynamics in various of DNA-based cellular processes, such as replication, transcription and DNA damage repair. Lysine succinylation (Ksucc) is a newly identified histone PTM, but its regulation and f...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa663
更新日期:2020-09-25 00:00:00
abstract::Native nucleotides show a hyperbolic concentration dependence of the pre-steady-state rate of incorporation while maintaining concentration-independent amplitude due to fast, largely irreversible pyrophosphate release. The kinetics of 3'-azido-2',3'-dideoxythymidine (AZT) incorporation exhibit an increase in amplitude...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm695
更新日期:2007-01-01 00:00:00
abstract::BRCA1 expression is repressed by aberrant cytosine methylation in sporadic breast cancer. We hypothesized that aberrant cytosine methylation of the BRCA1 promoter was associated with the transcriptionally repressive effects of histone hypoacetylation and chromatin condensation. To address this question, we developed a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.17.3233
更新日期:2000-09-01 00:00:00
abstract::Spliceosomes are assembled in stages. The first stage forms complex E, which is characterized by the presence of U1 snRNPs base-paired to the 5' splice site, components recognizing the 3' splice site and proteins thought to connect them. The splice sites are held in close proximity and the pre-mRNA is committed to spl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks322
更新日期:2012-08-01 00:00:00
abstract::Patients with Alzheimer's disease (AD) exhibit higher levels of 8-oxo-guanine (8-oxoG) DNA lesions in their brain, suggesting a reduced or defective 8-oxoG repair. To test this hypothesis, this study investigated 14 AD patients and 10 age-matched controls for mutations of the major 8-oxoG removal gene OGG1. Whereas no...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkm189
更新日期:2007-01-01 00:00:00
abstract::Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented DNA, information traditionally lost in standard NGS library prepa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa128
更新日期:2020-05-07 00:00:00
abstract::Archaeal family-B DNA polymerases bind tightly to deaminated bases and stall replication on encountering uracil in template strands, four bases ahead of the primer-template junction. Should the polymerase progress further towards the uracil, for example, to position uracil only two bases in front of the junction, 3'-5...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks1364
更新日期:2013-02-01 00:00:00
abstract::Abasic substitutions in the non-template strand and promoter sequence changes were made to assess the roles of various promoter features in sigma70 holoenzyme interactions with fork junction probes. Removal of -10 element non-template single strand bases, leaving the phosphodiester backbone intact, did not interfere w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg400
更新日期:2003-06-01 00:00:00
abstract::Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crys...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku229
更新日期:2014-05-01 00:00:00
abstract::In plant organelles, RNA editing alters specific cytidine residues to uridine in transcripts. All of the site-specificity factors of RNA editing identified so far are pentatricopeptide repeat (PPR) proteins. A defect in a specific PPR protein often impairs RNA editing at multiple sites, at which the cis-acting element...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks164
更新日期:2012-06-01 00:00:00
abstract::A somatic cell hybrid line containing only human chromosome 21 on a mouse background has been used as the source of DNA for construction of a recombinant phage library. Individual phages containing human inserts have been identified. Repeat-free human DNA subclones have been prepared and used to screen for restriction...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.17.6075
更新日期:1985-09-11 00:00:00
abstract::Genomic imbalances are a major cause of constitutional and acquired disorders. Therefore, aneuploidy screening has become the cornerstone of preimplantation, prenatal and postnatal genetic diagnosis, as well as a routine aspect of the diagnostic workup of many acquired disorders. Recently, array comparative genomic hy...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl336
更新日期:2006-05-12 00:00:00
abstract::Members of the HMG-I(Y) family of mammalian nonhistone proteins are of importance because they have been demonstrated to bind specifically to the minor groove of A.T-rich sequences both in vitro and in vivo and to function as gene transcriptional regulatory proteins in vivo. Here we report the cloning, sequencing, cha...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.18.4259
更新日期:1993-09-11 00:00:00
abstract::Initiation of Adenovirus (Ad) DNA replication occurs by a protein-priming mechanism in which the viral precursor terminal protein (pTP) and DNA polymerase (pol) as well as two nuclear DNA-binding proteins from uninfected HeLa cells are required. Biochemical studies on the pTP and DNA polymerase proteins separately hav...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.6.2431
更新日期:1988-03-25 00:00:00
abstract::We have determined the NMR solution structures of the quadruplexes formed by d(TGLGLT) and d(TL4T), where L denotes LNA (locked nucleic acid) modified G-residues. Both structures are tetrameric, parallel and right-handed and the native global fold of the corresponding DNA quadruplex is retained upon introduction of th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl144
更新日期:2006-04-13 00:00:00
abstract::A problem in EST clustering is the presence of repeat sequences. To avoid false matches, repeats have to be masked. This can be a time-consuming process, and it depends on available repeat libraries. We present a fast and effective method that aims to eliminate the problems repeats cause in the process of clustering. ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki511
更新日期:2005-04-14 00:00:00
abstract::Ribosome synthesis involves the concomitance of pre-rRNA processing and ribosomal protein assembly. In eukaryotes, this is a complex process that requires the participation of specific sequences and structures within the pre-rRNAs, at least 200 trans-acting factors and the ribosomal proteins. There is little informati...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq260
更新日期:2010-08-01 00:00:00
abstract::Cellular senescence, an integral component of aging and cancer, arises in response to diverse triggers, including telomere attrition, macromolecular damage and signaling from activated oncogenes. At present, senescent cells are identified by the combined presence of multiple traits, such as senescence-associated prote...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz555
更新日期:2019-08-22 00:00:00
abstract::The integrity and stability of nucleosomes under transcription assay conditions has been found to depend on concentration and ionic environment. Rifamycin AF/013, a commonly used inhibitor of initiation, is particularly effective in destabilisation of nucleosomes. Intact nucleosomes are refractory to transcription by ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/7.2.377
更新日期:1979-09-25 00:00:00
abstract::The human transcription factor EF-1A binds to the purine-rich E1A core enhancer sequence in the adenovirus E1A and E4 and polyomavirus enhancer regions. The consensus binding site for EF-1A resembles that of members of the ets domain protein family. EF-1A activation of transcription requires a dimeric binding site. An...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.24.6555
更新日期:1992-12-25 00:00:00
abstract::In this article, we characterize histone demethylase activity of the entire family of JmjC+N proteins of Drosophila melanogaster. Our results show that Lid (little imaginal discs), which is structurally homologous to JARID1, demethylates H3K4me3. However, contrary to what would be inferred from its demethylase activit...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn098
更新日期:2008-05-01 00:00:00
abstract::Analysis of 41 histone homologous clones from an isogenic gene library of Drosophila melanogaster showed that non-histone fragments interrupt the histone repetitive clusters at several sites. Long (L) and short (S) forms of the repeating units are distinguished by the insertion of 240 bp into the spacer between H1 and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.1.225
更新日期:1989-01-11 00:00:00
abstract::We have previously shown that integration of a polyoma vector containing rodent repetitive elements into rat cellular DNA is non-random (Wallenburg et al. J. Virol. 50: 678-683). Junctions between the polyoma vector and the host DNA occur in the repetitive sequences of the vector about ten times more frequently than w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.19.7849
更新日期:1987-10-12 00:00:00
abstract::In order to study the effects of DNA structure on cellular processes such as transcription, we have made a series of plasmids that locate several different kinds of DNA structure (stiff, flexible or curved) near the sites of cleavage by commonly-used restriction enzymes. One can use these plasmids to place any DNA reg...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.22.9447
更新日期:1989-11-25 00:00:00
abstract::RNA editing of miRNAs, especially in the seed region, adds another layer to miRNA mediated gene regulation which can modify its targets, altering cellular signaling involved in important processes such as differentiation. In this study, we have explored the role of miRNA editing in CD4(+) T cell differentiation. CD4(+...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv752
更新日期:2015-09-18 00:00:00
abstract::The reactions between purified anti-poly A. poly U and-poly I. poly C. antibodies (IgG and IgM), and synthetic and natural polynucleotides were visualized at the molecular level. This was achieved by the use of fine tungsten bidirectional shadowing of molecules adsorbed onto thin carbon films, combined with dark field...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.8.1805
更新日期:1980-04-25 00:00:00
abstract::To investigate the mechanism of N4-aminocytidine-induced mutagenesis, N'-alkyl-N4-aminocytidines and N4-alkyl-N4-aminocytidines were prepared and their mutagenicity on bacteria were assayed. N'-Methyl-N4-aminocytidine, N'-(2-hydroxyethyl)-N4-aminocytidine and N',N'-dimethyl-N4-aminocytidine showed direct-acting mutage...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.24.8893
更新日期:1985-12-20 00:00:00
abstract::The loss of the tumour suppressor E-cadherin (Cdh1) is a key event during tumourigenesis and epithelial-mesenchymal transition (EMT). Transforming growth factor-β (TGFβ) triggers EMT by inducing the expression of non-histone chromatin protein High Mobility Group A2 (HMGA2). We have previously shown that HMGA2, togethe...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1293
更新日期:2015-01-01 00:00:00