HapZipper: sharing HapMap populations just got easier.

abstract：:We have developed a novel method of genetic library construction on magnetic microbeads based on solid-phase single-molecule PCR in a fine and robust water-phase compartment formed in water-in-oil (w/o) emulsions. In this method, critically diluted DNA fragments were distributed over the emulsion as templates, where b...

journal_title：Nucleic acids research

authors： Kojima T,Takei Y,Ohtsuka M,Kawarasaki Y,Yamane T,Nakano H

更新日期：2005-10-06 00:00:00

abstract：:The DNA photosensitisers m-iodo Hoechst and m-iodo, p-methoxy Hoechst have been co-crystallised with the oligonucleotide d(CGCGAATTCGCG)(2)and their crystal structures determined. The crystals were then subjected to slow dehydration, which reduced their solvent contents from 40 (normal) to 30 (partially dehydrated) an...

journal_title：Nucleic acids research

authors： Squire CJ,Baker LJ,Clark GR,Martin RF,White J

更新日期：2000-03-01 00:00:00

abstract：:Eukaryotic topoisomerase II (topo II) is the essential decatenase of newly replicated chromosomes and the main relaxase of nucleosomal DNA. Apart from these general tasks, topo II participates in more specialized functions. In mammals, topo IIα interacts with specific RNA polymerases and chromatin-remodeling complexes...

journal_title：Nucleic acids research

authors： Nikolaou C,Bermúdez I,Manichanh C,García-Martinez J,Guigó R,Pérez-Ortín JE,Roca J

更新日期：2013-11-01 00:00:00

abstract：:RNA pseudoknots are functional structure elements with key roles in viral and cellular processes. Prediction of a pseudoknotted minimum free energy structure is an NP-complete problem. Practical algorithms for RNA structure prediction including restricted classes of pseudoknots suffer from high runtime and poor accura...

journal_title：Nucleic acids research

authors： Sperschneider J,Datta A

更新日期：2010-04-01 00:00:00

abstract：:Three computer programs are described which may be used to translate a DNA sequence into a protein sequence, back translate the protein sequence into an ambiguous DNA sequence, and then do pattern searching in the ambiguous sequence. The programs are written in the C programming language, have been compiled to run on ...

journal_title：Nucleic acids research

authors： Mount DW,Conrad B

更新日期：1984-01-11 00:00:00

abstract：:The reported binding preference of human hnRNP protein A1 for the 3'-splice site of some introns (Swanson and Dreyfuss (1988) EMBO J. 7, 3519-3529; Mayrand and Pederson (1990) Nucleic Acids Res. 18, 3307-3318) was tested by assaying in vitro the binding of purified recombinant A1 protein (expressed in bacteria) to syn...

journal_title：Nucleic acids research

authors： Buvoli M,Cobianchi F,Biamonti G,Riva S

更新日期：1990-11-25 00:00:00

abstract：:A number of cysteine-rich proteins have recently been isolated by homology screening, differential library screens, and association with other proteins. In this report, we describe the isolation of the rat cysteine-rich protein from a rat brain library during a search for clones with homology to the delta-opioid recep...

journal_title：Nucleic acids research

authors： McLaughlin CR,Tao Q,Abood ME

更新日期：1994-12-11 00:00:00

abstract：:CeR-2 RNA is one of the newly identified Caenorhabditis elegans noncoding RNAs (ncRNAs). The characterization of CeR-2 by RNomic studies has failed to classify it into any known ncRNA family. In this study, we examined the spatiotemporal expression patterns of CeR-2 to gain insight into its function. CeR-2 is expresse...

journal_title：Nucleic acids research

authors： Hokii Y,Sasano Y,Sato M,Sakamoto H,Sakata K,Shingai R,Taneda A,Oka S,Himeno H,Muto A,Fujiwara T,Ushida C

更新日期：2010-09-01 00:00:00

abstract：:Long Interspersed Element-1 (LINE-1) retrotransposition contributes to inter- and intra-individual genetic variation and occasionally can lead to human genetic disorders. Various strategies have been developed to identify human-specific LINE-1 (L1Hs) insertions from short-read whole genome sequencing (WGS) data; howev...

journal_title：Nucleic acids research

authors： Zhou W,Emery SB,Flasch DA,Wang Y,Kwan KY,Kidd JM,Moran JV,Mills RE

更新日期：2020-02-20 00:00:00

abstract：:Transcription factor binding sites (TFBSs) are short DNA sequences interacting with transcription factors (TFs), which regulate gene expression. Due to the relatively short length of such binding sites, it is largely unclear how the specificity of protein-DNA interaction is achieved. Here, we have performed a genome-w...

journal_title：Nucleic acids research

authors： Zhang C,Xuan Z,Otto S,Hover JR,McCorkle SR,Mandel G,Zhang MQ

更新日期：2006-05-02 00:00:00

abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that mediate gene expression at the post-transcriptional and translational levels by an imperfect binding to target mRNA 3'UTR regions. While the ab-initio computational prediction of miRNA-mRNA interactions still poses significant challenges, it is possible to overcome som...

journal_title：Nucleic acids research

authors： Sales G,Coppe A,Bicciato S,Bortoluzzi S,Romualdi C

更新日期：2010-04-01 00:00:00

abstract：:A complete physical map of the spinach mitochondrial genome has been established. The entire sequence content of 327 kilobase pairs (kb) is postulated to occur as a single circular molecule. Two directly repeated elements of approximately 6 kb, located on this "master chromosome", are proposed to participate in an int...

journal_title：Nucleic acids research

authors： Stern DB,Palmer JD

更新日期：1986-07-25 00:00:00

abstract：:We have determined the three-dimensional structure of a non-selfcomplementary nonanucleotide duplex which contains an abasic (apyrimidinic) site in the centre, i.e. a deoxyribose residue opposite an adenosine. The majority of the base and sugar proton resonances were assigned by NOESY, COSY and 2DQF spectra in D2O and...

journal_title：Nucleic acids research

authors： Cuniasse P,Sowers LC,Eritja R,Kaplan B,Goodman MF,Cognet JA,LeBret M,Guschlbauer W,Fazakerley GV

更新日期：1987-10-12 00:00:00

abstract：:MicroRNA 26a (miR-26a) reduces cell viability in several cancers, indicating that miR-26a could be used as a therapeutic option in patients. We demonstrate that miR-26a not only inhibits G1-S cell cycle transition and promotes apoptosis, as previously described, but also regulates multiple cell cycle checkpoints. We s...

journal_title：Nucleic acids research

authors： Castellano L,Dabrowska A,Pellegrino L,Ottaviani S,Cathcart P,Frampton AE,Krell J,Stebbing J

更新日期：2017-05-05 00:00:00

abstract：:The R-Coffee web server produces highly accurate multiple alignments of noncoding RNA (ncRNA) sequences, taking into account predicted secondary structures. R-Coffee uses a novel algorithm recently incorporated in the T-Coffee package. R-Coffee works along the same lines as T-Coffee: it uses pairwise or multiple seque...

journal_title：Nucleic acids research

authors： Moretti S,Wilm A,Higgins DG,Xenarios I,Notredame C

更新日期：2008-07-01 00:00:00

abstract：:We have prepared several novel phosphoramidites and have synthesised oligonucleotides incorporating them internally. The presence of these residues in an oligonucleotide template presents an impossible barrier to primed synthesis by Taq DNA polymerase. When extended as polymerase chain reaction products, these oligonu...

journal_title：Nucleic acids research

authors： Newton CR,Holland D,Heptinstall LE,Hodgson I,Edge MD,Markham AF,McLean MJ

更新日期：1993-03-11 00:00:00

abstract：:Ribosome-inactivating proteins (RIP) are a family of plant enzymes for which a unique activity was determined: rRNAN-glycosidase at a specific universally conserved position, A4324in the case of rat ribosomes. Recently we have shown that the RIP from Saponaria officinalis have a much wider substrate specificity: they ...

journal_title：Nucleic acids research

authors： Barbieri L,Valbonesi P,Bonora E,Gorini P,Bolognesi A,Stirpe F

更新日期：1997-02-01 00:00:00

abstract：:Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the ...

journal_title：Nucleic acids research

authors： Charlieu JP,Laurent AM,Carter DA,Bellis M,Roizès G

更新日期：1992-03-25 00:00:00

abstract：:Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and...

journal_title：Nucleic acids research

authors： Tsai-Morris CH,Cao XM,Sukhatme VP

更新日期：1988-09-26 00:00:00

abstract：:The Génolevures online database (http://cbi.labri.fr/Genolevures/ and http://genolevures.org/) provides exploratory tools and curated data sets relative to nine complete and seven partial genome sequences determined and manually annotated by the Génolevures Consortium, to facilitate comparative genomic studies of Hemi...

journal_title：Nucleic acids research

authors： Sherman DJ,Martin T,Nikolski M,Cayla C,Souciet JL,Durrens P,Génolevures Consortium.

更新日期：2009-01-01 00:00:00

abstract：:Reliable prediction of orthology is central to comparative genomics. Approaches based on phylogenetic analyses closely resemble the original definition of orthology and paralogy and are known to be highly accurate. However, the large computational cost associated to these analyses is a limiting factor that often preve...

journal_title：Nucleic acids research

authors： Pryszcz LP,Huerta-Cepas J,Gabaldón T

更新日期：2011-03-01 00:00:00

abstract：:The RNA bacteriophages of E. coli specifically encapsidate a single copy of the viral genome in a protein shell composed mainly of 180 molecules of coat protein. Coat protein is also a translational repressor and shuts off viral replicase synthesis by interaction with a RNA stem-loop containing the replicase initiatio...

journal_title：Nucleic acids research

authors： Pickett GG,Peabody DS

更新日期：1993-09-25 00:00:00

abstract：:Signal recognition particle (SRP) is a stable cytoplasmic ribonucleoprotein complex that serves to translocate secretory proteins across membranes during translation. The SRP Database (SRPDB) provides compilations of SRP components, ordered alphabetically and phylogenetically. Alignments emphasize phylogenetically-sup...

journal_title：Nucleic acids research

authors： Gorodkin J,Knudsen B,Zwieb C,Samuelsson T

更新日期：2001-01-01 00:00:00

abstract：:Translational repression (TR) plays an important role in post-transcriptional regulation of gene expression and embryonic development in metazoans. TR also regulates the expression of a subset of the cytoplasmic mRNA population during development of fertilized female gametes of the unicellular malaria parasite, Plasmo...

journal_title：Nucleic acids research

authors： Braks JA,Mair GR,Franke-Fayard B,Janse CJ,Waters AP

更新日期：2008-03-01 00:00:00

abstract：:Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and...

journal_title：Nucleic acids research

authors： Kang JJ,Watson RM,Fisher ME,Higuchi R,Gelfand DH,Holland MJ

更新日期：2000-01-15 00:00:00

abstract：:The design of a family shuffling strategy (CLERY: Combinatorial Libraries Enhanced by Recombination in Yeast) associating PCR-based and in vivo recombination and expression in yeast is described. This strategy was tested using human cytochrome P450 CYP1A1 and CYP1A2 as templates, which share 74% nucleotide sequence id...

journal_title：Nucleic acids research

authors： Abécassis V,Pompon D,Truan G

更新日期：2000-10-15 00:00:00

abstract：:Chromatin immunoprecipitation (ChIP) has ushered in a new era of scientific discovery by allowing new insights into DNA-protein interactions. ChIP is used to quantify enriched genomic regions using qPCR, and more recently is combined with next generation sequencing (ChIP-seq) to obtain a genome wide profile of protein...

journal_title：Nucleic acids research

authors： Kurtenbach S,Reddy R,Harbour JW

更新日期：2019-01-08 00:00:00

abstract：:RNase III enzymes cleave double stranded (ds)RNA. This is an essential step for regulating the processing of mRNA, rRNA, snoRNA and other small RNAs, including siRNA and miRNA. Arabidopsis thaliana encodes nine RNase III: four DICER-LIKE (DCL) and five RNASE THREE LIKE (RTL). To better understand the molecular functio...

journal_title：Nucleic acids research

authors： Charbonnel C,Niazi AK,Elvira-Matelot E,Nowak E,Zytnicki M,de Bures A,Jobet E,Opsomer A,Shamandi N,Nowotny M,Carapito C,Reichheld JP,Vaucheret H,Sáez-Vásquez J

更新日期：2017-11-16 00:00:00

abstract：:Nitrated polycyclic aromatic hydrocarbons are common environmental pollutants, of which many are mutagenic and carcinogenic. 1-Nitropyrene is the most abundant nitrated polycyclic aromatic hydrocarbon, which causes DNA damage and is carcinogenic in experimental animals. Error-prone translesion synthesis of 1-nitropyre...

journal_title：Nucleic acids research

authors： Kirouac KN,Basu AK,Ling H

更新日期：2013-02-01 00:00:00

abstract：:Understanding the normal state of human tissue transcriptome profiles is essential for recognizing tissue disease states and identifying disease markers. Recently, the Human Protein Atlas and the FANTOM5 consortium have each published extensive transcriptome data for human samples using Illumina-sequenced RNA-Seq and ...

journal_title：Nucleic acids research

authors： Yu NY,Hallström BM,Fagerberg L,Ponten F,Kawaji H,Carninci P,Forrest AR,Fantom Consortium.,Hayashizaki Y,Uhlén M,Daub CO

更新日期：2015-08-18 00:00:00