Abstract：
:The RNA bacteriophages of E. coli specifically encapsidate a single copy of the viral genome in a protein shell composed mainly of 180 molecules of coat protein. Coat protein is also a translational repressor and shuts off viral replicase synthesis by interaction with a RNA stem-loop containing the replicase initiation codon. We wondered whether the translational operator also serves as the viral pac site, the signal which mediates the exclusive encapsidation of viral RNA by its interaction with coat protein. To test this idea we measured the ability of lacZ RNA fused to the translational operator to be incorporated into virus-like particles formed from coat protein expressed from a plasmid. The results indicate that the operator-lacZ RNA is indeed encapsidated and that nucleotide substitutions in the translational operator which reduce the tightness of the coat protein-operator interaction also reduce or abolish encapsidation of the hybrid RNA. When coat protein is expressed in excess compared to the operator-lacZ RNA, host RNAs are packaged as well. However, elevation of the level of operator-lacZ RNA relative to coat protein results in its selective encapsidation at the expense of cellular RNAs. Our results are consistent with the proposition that this single protein-RNA interaction accounts both for translational repression and viral genome encapsidation.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Pickett GG,Peabody DSdoi
10.1093/nar/21.19.4621subject
Has Abstractpub_date
1993-09-25 00:00:00pages
4621-6issue
19eissn
0305-1048issn
1362-4962journal_volume
21pub_type
杂志文章abstract：:Type I toxin-antitoxin (TA) systems have been identified in a wide range of bacterial genomes. Here, we report the characterization of a new type I TA system present on the chromosome of the major human gastric pathogen, Helicobacter pylori. We show that the aapA1 gene encodes a 30 amino acid peptide whose artificial ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw1343
更新日期：2017-05-05 00:00:00
abstract：:The centromere is a highly specialized chromosomal element that is essential for chromosome segregation during mitosis. Centromere integrity must therefore be properly preserved and is strictly dependent upon the establishment and maintenance of surrounding chromatin structure. Here we identify WDHD1, a WD40-domain an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq1338
更新日期：2011-05-01 00:00:00
abstract：:The MyHits web site (http://myhits.isb-sib.ch) is an integrated service dedicated to the analysis of protein sequences. Since its first description in 2004, both the user interface and the back end of the server were improved. A number of tools (e.g. MAFFT, Jacop, Dotlet, Jalview, ESTScan) were added or updated to imp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm352
更新日期：2007-07-01 00:00:00
abstract：:We have identified cDNAs encoding Mel-N1, the mouse homologue of a human nervous system-specific RNA-binding protein, Hel-N1. Two major mRNA transcripts of Mel-N1 were detected predominantly in the adult mouse brain by Northern blot analysis. To gain insight into the RNA binding specificity of Mel-N1, we performed ite...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.11.2011
更新日期：1996-06-01 00:00:00
abstract：:We report the use of MonoQ FPLC (Fast Protein Liquid Chromatography) for the rapid purification of a novel Type II restriction endonuclease PmaCI, from Pseudomonas maltophila, which recognises the sequence 5'-CAC decreases GTG-3'. The resulting enzyme is free of other nucleases to a level suitable for its characterisa...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.3.1293
更新日期：1986-02-11 00:00:00
abstract：:Human stem cells are promising sources for regenerative therapy. To ensure safety of future therapeutic applications, the differentiation potency of stem cells has to be tested and be widely opened to the public. The potency is generally assessed by teratoma formation comprising differentiated cells from all three ger...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1096
更新日期：2016-01-04 00:00:00
abstract：:Gene finding is complicated in organisms that exhibit insertional RNA editing. Here, we demonstrate how our new algorithm Predictor of Insertional Editing (PIE) can be used to locate genes whose mRNAs are subjected to multiple frameshifting events, and extend the algorithm to include probabilistic predictions for site...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki820
更新日期：2005-09-07 00:00:00
abstract：:LTR units associated with cellular retrovirus-like elements are abundantly present in chromosomal DNA of animal cells. We have analyzed the promoter and enhancer activities of diverse LTR units associated with different members of the murine retrovirus-like family known as VL30. We report here that the structurally he...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.2.645
更新日期：1986-01-24 00:00:00
abstract：:Biological evolution resulted in a homochiral world in which nucleic acids consist exclusively of d-nucleotides and proteins made by ribosomal translation of l-amino acids. From the perspective of synthetic biology, however, particularly anabolic enzymes that could build the mirror-image counterparts of biological mac...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx079
更新日期：2017-04-20 00:00:00
abstract：:The DNA damage checkpoint halts cell cycle progression in G2 in response to genotoxic insults. Central to the execution of cell cycle arrest is the checkpoint-induced stabilization of securin-separase complex (yeast Pds1-Esp1). The checkpoint kinases Chk1 and Chk2 (yeast Chk1 and Rad53) are thought to critically contr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa355
更新日期：2020-06-19 00:00:00
abstract：:7,8-Dihydro-8-oxo-deoxyguanosine (8oxodG) is a highly premutagenic DNA lesion due to its ability to mispair with adenine. Schizosaccharomyces pombe lacks homologs for relevant enzymes that repair 8oxodG, which suggests that this lesion could be persistent and must be tolerated. Here we show that SpPol4, the unique Pol...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku711
更新日期：2014-09-01 00:00:00
abstract：:The Genomes On Line Database (GOLD) is a web resource for comprehensive access to information regarding complete and ongoing genome sequencing projects worldwide. The database currently incorporates information on over 1500 sequencing projects, of which 294 have been completed and the data deposited in the public data...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkj145
更新日期：2006-01-01 00:00:00
abstract：:Differential scanning microcalorimetry (DSC), UV absorption and circular dichroism (CD) have been used to study structure and stability of linear (lin), open circular (oc), supercoiled (cd) and relaxed circular duplex (rd) DNA and calf thymus (CT) DNA. Investigations were made in low salt buffer and in the presence of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.24.11737
更新日期：1988-12-23 00:00:00
abstract：:Mixed-lineage leukemia (MLL) maintains the expression of cellular memory genes during development, while leukemic MLL fusion proteins aberrantly maintain expression of hematopoietic stem cell program genes such as HOXA9 to cause leukemia. However, the molecular mechanism of gene activation is unclear. Here we show tha...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt1394
更新日期：2014-04-01 00:00:00
abstract：:We have isolated an eight kilobase fragment of Bacillus subtilis DNA by specific integration and excision of a plasmid containing a sequence adjacent to ribosomal operon rrn O. The genetic locus of the cloned fragment was verified by linkage of the integrated vector to nearby genetic markers using both transduction an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.15.6307
更新日期：1984-08-10 00:00:00
abstract：:Lambda-red system-based recombinogenic engineering is a powerful new method to engineer DNA without the need for restriction enzymes or ligases. Here, we report the use of a single selectable marker to enhance the usefulness of this approach. The strategy is to utilize the thymidylate synthase A (thyA) gene, which enc...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gni059
更新日期：2005-03-30 00:00:00
abstract：:In mammalian cells, DNA double-strand breaks (DSBs) are repaired by three pathways, nonhomologous end-joining (NHEJ), gene conversion (GC) and single-strand annealing (SSA). These pathways are distinct with regard to repair efficiency and mutagenic potential and must be tightly controlled to preserve viability and gen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkn347
更新日期：2008-07-01 00:00:00
abstract：:The 'regions' method for multisequence alignment used in the previously reported program MALIGN has been generalized to include recursive refinement so that unaligned portions between two regions at the current level of resolution can be handled with increased resolution. Additionally, there is incorporated a limiting...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.5.1683
更新日期：1988-03-11 00:00:00
abstract：:Adaptation of eucaryotic cDNA to heterologous expression was studied by mutating the translation initiation (TI) region upstream (mTI) and downstream (MTI) of the start codon. In the mTI subregion the 8 bases flanking the invariant Shine-Dalgarno motif GG-AG were mutagenized exhaustively, while the MTI subregion was s...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.12.3127
更新日期：1992-06-25 00:00:00
abstract：:The gene for the small subunit of human calcium-activated neutral protease was isolated and sequenced. It is 11 kb long and comprises 11 exons. No TATA or CAT box was found upstream of the possible transcription initiation sites, but there are three so-called G-C box sequences and one G-C box-like sequence, which are ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.22.8805
更新日期：1986-11-25 00:00:00
abstract：:Insertion of microRNA target sequences into the flavivirus genome results in selective tissue-specific attenuation and host-range restriction of live attenuated vaccine viruses. However, previous strategies for miRNA-targeting did not incorporate a mechanism to prevent target elimination under miRNA-mediated selective...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkw061
更新日期：2016-04-20 00:00:00
abstract：:We have measured the amount of 5S-ribosomal DNA in the genomes of Xenopus laevis, Triturus cristatus carnifex and Ambystoma mexicanum, three species of Amphibians which have widely different C-values. Our best estimate is that these organisms have about 24,000, 32,000 and 61,000 5S-genes per haploid genome respectivel...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.8.2381
更新日期：1983-04-25 00:00:00
abstract：:Human telomerase reverse transcriptase (hTERT), the catalytic subunit of human telomerase, contains conserved motifs common to retroviral reverse transcriptases and telomerases. Within the C motif of hTERT is the Leu866-Val867-Asp868-Asp869 tetrapeptide that includes a catalytically essential aspartate dyad. Site-dire...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm002
更新日期：2007-01-01 00:00:00
abstract：:A large number of signalling pathways converge on p53 to induce different cellular stress responses that aim to promote cell cycle arrest and repair or, if the damage is too severe, to induce irreversible senescence or apoptosis. The differentiation of p53 activity towards specific cellular outcomes is tightly regulat...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkz124
更新日期：2019-04-23 00:00:00
abstract：:The Transcription Regulatory Regions Database (TRRD) is a curated database designed for accumulation of experimental data on extended regulatory regions of eukaryotic genes, the regulatory elements they contain, i.e., transcription factor binding sites, promoters, enhancers, silencers, etc., and expression patterns of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.1.303
更新日期：1999-01-01 00:00:00
abstract：:AR9 is a giant Bacillus subtilis phage whose uracil-containing double-stranded DNA genome encodes distant homologs of β and β' subunits of bacterial RNA polymerase (RNAP). The products of these genes are thought to assemble into two non-canonical multisubunit RNAPs - a virion RNAP (vRNAP) that is injected into the hos...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx264
更新日期：2017-06-02 00:00:00
abstract：:Protein complexes are key molecular entities that integrate multiple gene products to perform cellular functions. The CORUM (http://mips.gsf.de/genre/proj/corum/index.html) database is a collection of experimentally verified mammalian protein complexes. Information is manually derived by critical reading of the scient...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkm936
更新日期：2008-01-01 00:00:00
abstract：:We have used gel retardation analysis to show that human DNA topoisomerase IIbeta can bind a 40 bp linear duplex containing a single DNA topoisomerase IIbeta cleavage site. Furthermore, we demonstrate for the first time that human DNA topoisomerase IIbeta binds to four-way junction DNA. This supports previous suggesti...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/27.4.984
更新日期：1999-02-15 00:00:00
abstract：:We have generated transgenic mice that express a diverse repertoire of human sequence immunoglobulins. The expression of this repertoire is directed by light and heavy chain minilocus transgenes comprised of human protein coding sequences in an unrearranged, germ-line configuration. In this paper we describe the const...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/20.23.6287
更新日期：1992-12-11 00:00:00
abstract：:Co-option of cis-regulatory modules has been suggested as a mechanism for the evolution of expression sites during development. However, the extent and mechanisms involved in mobilization of cis-regulatory modules remains elusive. To trace the history of non-coding elements, which may represent candidate ancestral cis...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt030
更新日期：2013-04-01 00:00:00
