The p53 mRNA: an integral part of the cellular stress response.

abstract：:The Bacillus cereus genome possesses three type IA topoisomerase genes. These genes, encoding DNA topoisomerase I and IIIalpha (bcTopo I, bcTopo IIIalpha), have been cloned into T7 RNA polymerase-regulated plasmid expression vectors and the enzymes have been overexpressed, purified and characterized. The proteins exhi...

journal_title：Nucleic acids research

authors： Li Z,Hiasa H,DiGate R

更新日期：2005-09-28 00:00:00

abstract：:By in vivo DMS footprint and reporter gene analyses we identified two transcription factor binding sites in the human c-sis/PDGF B gene promoter. The low basal activity of the PDGF B promoter in HeLa and undifferentiated K562 cells, which express low PDGF B mRNA levels, and in PC3 cells, which express a high PDGF B mR...

journal_title：Nucleic acids research

authors： Dirks RP,Jansen HJ,van Gerven B,Onnekink C,Bloemers HP

更新日期：1995-04-11 00:00:00

abstract：:We have developed an effective post-PCR sterilization process and have applied the procedure to a diagnostic assay for HIV-1. The method, which is based on isopsoralen photochemistry, satisfies both the inactivation and hybridization requirements of a practical sterilization process. The key feature of the technique i...

journal_title：Nucleic acids research

authors： Isaacs ST,Tessman JW,Metchette KC,Hearst JE,Cimino GD

更新日期：1991-01-11 00:00:00

abstract：:The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact ...

journal_title：Nucleic acids research

authors： Guigues M,Leng M

更新日期：1976-12-01 00:00:00

abstract：:Despite the development of new high-throughput sequencing techniques, microarrays are still attractive tools to study small genome organisms, thanks to sample multiplexing and high-feature densities. However, the oligonucleotide design remains a delicate step for most users. A vast array of software is available to de...

journal_title：Nucleic acids research

authors： Jourdren L,Duclos A,Brion C,Portnoy T,Mathis H,Margeot A,Le Crom S

更新日期：2010-06-01 00:00:00

abstract：:Peroxisomes are essential organelles of eukaryotic origin, ubiquitously distributed in cells and organisms, playing key roles in lipid and antioxidant metabolism. Loss or malfunction of peroxisomes causes more than 20 fatal inherited conditions. We have created a peroxisomal database (http://www.peroxisomeDB.org) that...

journal_title：Nucleic acids research

authors： Schlüter A,Fourcade S,Domènech-Estévez E,Gabaldón T,Huerta-Cepas J,Berthommier G,Ripp R,Wanders RJ,Poch O,Pujol A

更新日期：2007-01-01 00:00:00

abstract：:Conserved in a variety of evolutionarily divergent plant species, LOB DOMAIN (LBD) genes define a large, plant-specific family of largely unknown function. LBD genes have been implicated in a variety of developmental processes in plants, although to date, relatively few members have been assigned functions. LBD protei...

journal_title：Nucleic acids research

authors： Husbands A,Bell EM,Shuai B,Smith HM,Springer PS

更新日期：2007-01-01 00:00:00

abstract：:Despite systematic approaches to mapping networks of genetic interactions in Saccharomyces cerevisiae, exploration of genetic interactions on a genome-wide scale has been limited. The S. cerevisiae haploid genome has 110 regions that are longer than 10 kb but harbor only non-essential genes. Here, we attempted to dele...

journal_title：Nucleic acids research

authors： Kaboli S,Yamakawa T,Sunada K,Takagaki T,Sasano Y,Sugiyama M,Kaneko Y,Harashima S

更新日期：2014-09-01 00:00:00

abstract：:Escherichia coli DNA polymerase III holoenzyme is composed of 10 different subunits linked by noncovalent interactions. The polymerase activity resides in the alpha-subunit. The epsilon-subunit, which contains the proofreading exonuclease site within its N-terminal 185 residues, binds to alpha via a segment of 57 addi...

journal_title：Nucleic acids research

authors： Ozawa K,Jergic S,Park AY,Dixon NE,Otting G

更新日期：2008-09-01 00:00:00

abstract：:Several authors have suggested or inferred that modest changes in microRNA expression can potentiate or impinge on their capacity to mediate gene repression, and that doing so could play a significant role in diseases. Such interpretations are based on several assumptions, namely: (i) changes in microRNA expression co...

journal_title：Nucleic acids research

authors： Mayya VK,Duchaine TF

更新日期：2015-09-03 00:00:00

abstract：:Oxanine (O) is a deamination product derived from guanine with the nitrogen at the N1 position substituted by oxygen. Cytosine, thymine, adenine, guanine as well as oxanine itself can be incorporated by Klenow Fragment to pair with oxanine in a DNA template with similar efficiency, indicating that oxanine in DNA may c...

journal_title：Nucleic acids research

authors： Hitchcock TM,Gao H,Cao W

更新日期：2004-08-02 00:00:00

abstract：:The opening of DNA double strands is extremely relevant to several biological functions, such as replication and transcription or binding of specific proteins. Such opening phenomenon is particularly sensitive to the aqueous solvent conditions in which the DNA molecule is dispersed, as it can be observed by considerin...

journal_title：Nucleic acids research

authors： Tempestini A,Cassina V,Brogioli D,Ziano R,Erba S,Giovannoni R,Cerrito MG,Salerno D,Mantegazza F

更新日期：2013-02-01 00:00:00

abstract：:The H19 lncRNA has been implicated in development and growth control and is associated with human genetic disorders and cancer. Acting as a molecular sponge, H19 inhibits microRNA (miRNA) let-7. Here we report that H19 is significantly decreased in muscle of human subjects with type-2 diabetes and insulin resistant ro...

journal_title：Nucleic acids research

authors： Gao Y,Wu F,Zhou J,Yan L,Jurczak MJ,Lee HY,Yang L,Mueller M,Zhou XB,Dandolo L,Szendroedi J,Roden M,Flannery C,Taylor H,Carmichael GG,Shulman GI,Huang Y

更新日期：2014-12-16 00:00:00

abstract：:Transcription factors are important gene regulators with distinctive roles in development, cell signaling and cell cycling, and they have been associated with many diseases. The ConTra v3 web server allows easy visualization and exploration of predicted transcription factor binding sites (TFBSs) in any genomic region ...

journal_title：Nucleic acids research

authors： Kreft L,Soete A,Hulpiau P,Botzki A,Saeys Y,De Bleser P

更新日期：2017-07-03 00:00:00

abstract：:The contacts of phosphate groups in mRNAs with ribosomes were studied. Two mRNAs were used: one mRNA contained in the middle two defined codons to construct the pre- and the post-translocational states, the other was a sequence around the initiation site of the natural cro-mRNA. Phosphorothioate nucleotides were rando...

journal_title：Nucleic acids research

authors： Alexeeva EV,Shpanchenko OV,Dontsova OA,Bogdanov AA,Nierhaus KH

更新日期：1996-06-15 00:00:00

abstract：:In the most extensive examination to date of the relationship between the pausing of reverse transcrip-tase (RT) and RNA secondary structures, pause events were found to be correlated to inverted repeats both ahead of, and behind the catalytic site in vitro. In addition pausing events were strongly associated with pol...

journal_title：Nucleic acids research

authors： Harrison GP,Mayo MS,Hunter E,Lever AM

更新日期：1998-07-15 00:00:00

abstract：:Fundamental aspects of the biochemistry of Type III restriction endonucleases remain unresolved despite being characterized by numerous research groups in the past decades. One such feature is the subunit stoichiometry of these hetero-oligomeric enzyme complexes, which has important implications for the reaction mecha...

journal_title：Nucleic acids research

authors： Butterer A,Pernstich C,Smith RM,Sobott F,Szczelkun MD,Tóth J

更新日期：2014-04-01 00:00:00

abstract：:A phosphotriester solid phase method on a polyamide support has been used to prepare oligodeoxyribonucleotides up to 12 units long. Compared to solid phase phosphodiester synthesis the new methodology is quicker, more flexible and gives 10-60-fold better overall yields. ...

journal_title：Nucleic acids research

authors： Gait MJ,Singh M,Sheppard RC,Edge MD,Greene AR,Heathcliffe GR,Atkinson TC,Newton CR,Markham AF

更新日期：1980-03-11 00:00:00

abstract：:Procaryotic DNA polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. DNA polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. We have investigated the fidelity of pol...

journal_title：Nucleic acids research

authors： Abbotts J,Nishiyama Y,Yoshida S,Loeb LA

更新日期：1987-02-11 00:00:00

abstract：:A study of the synthesis of oligothymidylates via phosphotriester intermediates on a polystyrene support is described. The sequence involves condensation of a phenyl nucleoside-3' -phosphorochloridate with the 5'-hydroxyl group of the carrier bound oligonucleotide derivative in the presence of 1-methylimidazole. Condi...

journal_title：Nucleic acids research

authors： Pless RC,Letsinger RL

更新日期：1975-06-01 00:00:00

abstract：:Highly regulated induction systems enabling dose-dependent and reversible fine-tuning of protein expression output are beneficial for engineering complex biosynthetic pathways. To address this, we developed PhiReX, a novel red/far-red light-regulated protein expression system for use in Saccharomyces cerevisiae. PhiRe...

journal_title：Nucleic acids research

authors： Hochrein L,Machens F,Messerschmidt K,Mueller-Roeber B

更新日期：2017-09-06 00:00:00

abstract：:Nucleic acid double helices are stabilized by hydrogen bonding and stacking forces (a combination of hydrophobic, dispersive and electrostatic forces) of the base pairs in the helix. One would predict the hydrogen bonding contributions to increase and the stacking contributions to decrease as the water activity in the...

journal_title：Nucleic acids research

authors： Sen A,Nielsen PE

更新日期：2007-01-01 00:00:00

abstract：:Recognition of DNA by proteins depends on DNA sequence and structure. Often unanswered is whether the structure of naked DNA persists in a protein-DNA complex, or whether protein binding changes DNA shape. While X-ray structures of protein-DNA complexes are numerous, the structure of naked cognate DNA is seldom availa...

journal_title：Nucleic acids research

authors： Azad RN,Zafiropoulos D,Ober D,Jiang Y,Chiu TP,Sagendorf JM,Rohs R,Tullius TD

更新日期：2018-03-16 00:00:00

abstract：:Cell surface mannose 6-phosphate/insulin-like growth factor II receptors (M6P/IGF2R) bind and target exogenous insulin-like growth factor II (IGF2) to the prelysosomes where it is degraded. Loss of heterozygosity (LOH) for M6P/IGF2R is found in cancers, with mutational inactivation of the remaining allele. We exploite...

journal_title：Nucleic acids research

authors： Huang Z,Wen Y,Shandilya R,Marks JR,Berchuck A,Murphy SK

更新日期：2006-01-23 00:00:00

abstract：:We describe a new, broadly applicable methodology for screening in parallel interactions of RNA-binding proteins (RBPs) with large numbers of microRNA (miRNA) precursors and for determining their affinities in native form in the presence of cellular factors. The assays aim at identifying pre-miRNAs that are potentiall...

journal_title：Nucleic acids research

authors： Towbin H,Wenter P,Guennewig B,Imig J,Zagalak JA,Gerber AP,Hall J

更新日期：2013-02-01 00:00:00

abstract：:In the presence of S-adenosyl methionine the 5' terminal guanosine residue of in vitro synthesized reovirus mRNA becomes methylated at the 2'-OH position. In addition, 7-methyl guanylic acid is condensed covalently at the 5' terminus resulting in the formation of a 5' to 5' triphosphate bridge. Analysis of the 5' term...

journal_title：Nucleic acids research

authors： Faust M,Hastings KE,Millward S

更新日期：1975-08-01 00:00:00

abstract：:Fluorescence anisotropy and fluorescence correlation spectroscopy measurements of rhodamine-labeled DNA oligonucleotide duplexes have been used to determine equilibrium binding constants for DNA binding of the prokaryotic transcription activator protein NtrC. Measurements were made with wild-type NtrC from Escherichia...

journal_title：Nucleic acids research

authors： Sevenich FW,Langowski J,Weiss V,Rippe K

更新日期：1998-03-15 00:00:00

abstract：:To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...

journal_title：Nucleic acids research

authors： Heuchel R,Matthias P,Schaffner W

更新日期：1989-11-25 00:00:00

abstract：:Analysis of the human protein-tyrosine phosphatase (PTP) PTPRJ mRNA detected three in-frame AUGs at the 5'-end (starting at nt +14, +191 and +356) with no intervening stop codons. This tandem AUG arrangement is conserved between humans and the mouse and is unique among the genes of the classical PTPs. Until now it was...

journal_title：Nucleic acids research

authors： Karagyozov L,Godfrey R,Böhmer SA,Petermann A,Hölters S,Ostman A,Böhmer FD

更新日期：2008-08-01 00:00:00

abstract：:With the improved secreted protein prediction approach and comprehensive data sources, including Swiss-Prot, TrEMBL, RefSeq, Ensembl and CBI-Gene, we have constructed secretomes of human, mouse and rat, with a total of 18 152 secreted proteins. All the entries are ranked according to the prediction confidence. They we...

journal_title：Nucleic acids research

authors： Chen Y,Zhang Y,Yin Y,Gao G,Li S,Jiang Y,Gu X,Luo J

更新日期：2005-01-01 00:00:00