Abstract:
:The binding of anti poly(I). poly (C) Fab fragments to double or triple stranded polynucletides has been studied by fluorescence. Association constants were deduced from competition experiments. The comparison of the association constants leads to the conclusion that several atoms of the base residues do not interact with the amino acid residues of the binding site of Fab fragment while the hydroxyl groups of furanose rings interact. These results suggest that the Fab fragments do not bind to the major groove of the double stranded polynucleotides. An interaction between the C(2)O group of pyrimidine residues and Fab fragments cannot be excluded. Circular dichroism of poly(I). poly(C) or poly(I). poly(br5C)-Fab fragments complexes are very different from the circular dichroism of free polynucleotides which suggests a deformation of the polynucleotides bound to the Fab fragments.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Guigues M,Leng Mdoi
10.1093/nar/3.12.3337keywords:
subject
Has Abstractpub_date
1976-12-01 00:00:00pages
3337-47issue
12eissn
0305-1048issn
1362-4962journal_volume
3pub_type
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