Abstract：
:The TGGCA protein, the chicken homologue of HeLa cell NF-I, was purified to homogeneity from liver tissue by a procedure which includes preparative mobility shift electrophoresis (PMSE) as the final step. PMSE was here adjusted for the isolation of the TGGCA protein, but can be used as a general method to characterize the protein moiety of specific DNA-binding proteins. The TGGCA protein is a family of 6 protein species, which show minor differences in molecular weight from 36.8kd to 29.8kd. This microheterogeneity differs from the size distribution reported for HeLa cell NF-I polypeptides. All species of the TGGCA protein bind identically to a synthetic DNA-binding site and appear to be highly related in primary structure. We discuss the possible functional importance of this microheterogeneity.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Rupp RA,Sippel AEdoi
10.1093/nar/15.23.9707subject
Has Abstractpub_date
1987-12-10 00:00:00pages
9707-26issue
23eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract：:RNA synthesis and DNA replication cease after DNA damage. We studied RNA synthesis using an in situ run-on assay and found ribosomal RNA (rRNA) synthesis was inhibited 24 h after UV light, gamma radiation or DNA cross-linking by cisplatin in human cells. Cisplatin led to accumulation of cells in S phase. Inhibition of...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt502
更新日期：2013-08-01 00:00:00
abstract：:The high mobility group protein (HMG)-box is a DNA-binding domain found in many proteins that bind preferentially to DNA of irregular structures in a sequence-independent manner and can bend the DNA. We show here that GST-fusion proteins of HMG domains from HMG1 and HMG2 promote a triple-stranded complex formation bet...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.23.4733
更新日期：1996-12-01 00:00:00
abstract：:In this study, we show that silencing of CITED2 using small-hairpin RNA (shCITED2) induced DNA damage and reduction of ERCC1 gene expression in HEK293, HeLa and H1299 cells, even in the absence of cisplatin. In contrast, ectopic expression of ERCC1 significantly reduced intrinsic and induced DNA damage levels, and res...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv934
更新日期：2015-12-15 00:00:00
abstract：:We took advantage of the unusual genomic organization of the ciliate Oxytricha trifallax to screen for eukaryotic non-coding RNA (ncRNA) genes. Ciliates have two types of nuclei: a germ line micronucleus that is usually transcriptionally inactive, and a somatic macronucleus that contains a reduced, fragmented and rear...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr501
更新日期：2011-09-01 00:00:00
abstract：:The replication control circuit of the FII plasmids R1, R100 and R6-5 involves a small untranslated RNA molecule of about 93 nucleotides. This RNA, designated CopA RNA in plasmid R1, acts in vivo as a post-transcriptional inhibitor of the expression of the RepA protein whose synthesis is required for each round of pla...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.6.2523
更新日期：1986-03-25 00:00:00
abstract：:This work describes the organization, at the nucleotide sequence level, of genes flanking the junctions of the large single copy regions and the inverted repeats of Spinacia oleracea (spinach) and Nicotiana debneyi chloroplast DNAs. In both genomes, trnH1, the gene for tRNA-His(GUG) is located at the extremity of the ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.16.6547
更新日期：1984-08-24 00:00:00
abstract：:The mutations of 76 haemophilia B patients representing the whole population registered with the Malmö haemophilia centre (42) and referrals from the UK, were characterised. RFLP haplotype analysis of the defective genes indicated that 51 single base pair substitutions were definitely of independent origin and 27 of t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.11.3227
更新日期：1990-06-11 00:00:00
abstract：:The structures of the termini and their flanking regions of two human KpnI family members were investigated. The two differed in length, but the starting sequence at one terminal (defined as the 5' terminal) was found to be common to both members. The Alu family sequence was found in the 5' flanking regions. The KpnI ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.19.6837
更新日期：1983-10-11 00:00:00
abstract：:Small interfering RNAs (siRNAs) are promising new active compounds in gene medicine but the induction of non-specific immune responses following their delivery continues to be a serious problem. With the purpose of avoiding such effects chemically modified siRNAs are tested in screening assay but often only examining ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks033
更新日期：2012-05-01 00:00:00
abstract：:Direct labeling of RNA is an expedient method for labeling large quantities (e.g. micrograms) of target RNA for microarray analysis. We have developed an efficient labeling system that uses T4 RNA ligase to attach a 3'-biotinylated donor molecule to target RNA. Microarray analyses indicate that directly labeled RNA is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnh085
更新日期：2004-06-17 00:00:00
abstract：:Mature chicken erythrocyte polynucleosomes which are soluble at physiological ionic strength are enriched in beta-globin DNA sequences. Vitellogenin chromatin, which is not expressed in this tissue, is found in aggregation prone, salt insoluble chromatin. There is a direct correlation between the size of soluble fragm...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.3.1081
更新日期：1987-02-11 00:00:00
abstract：:Genome-wide association studies (GWAS) have become a preferred method to identify new genetic susceptibility loci. This technique aims to understanding the molecular etiology of common diseases, but in many cases, it has led to the identification of loci with no obvious biological relevance. Herein, we show that previ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks169
更新日期：2012-06-01 00:00:00
abstract：:Pre-mRNA transcripts in a variety of organisms, including plants, Drosophila and Caenorhabditis elegans, contain introns which are significantly richer in adenosine and uridine residues than their flanking exons. Previous analyses using exonic and intronic replacements between two nonequivalent 5'splice sites in the 4...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.5.1071
更新日期：1997-03-01 00:00:00
abstract：:The mammalian target of rapamycin (mTOR) is a critical regulator of cell growth, integrating multiple signalling cues and pathways. Key among the downstream activities of mTOR is the control of the protein synthesis machinery. This is achieved, in part, via the co-ordinated regulation of mRNAs that contain a terminal ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa1189
更新日期：2021-01-11 00:00:00
abstract：:When the function of origins of replication in yeast was compromised by placing ARS sequences downstream of strong promoters, ARS activity might have been affected either by transcription or by an altered chromatin configuration induced by the construct. To distinguish between these possibilities, derivatives of the y...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/22.19.3904
更新日期：1994-09-25 00:00:00
abstract：:Ditercalinium, an antitumor bifunctional intercalator which forms a high affinity reversible complex with DNA, was found to be specifically cytotoxic for polA and lig7 E. coli strains. In the polA strain, the cytotoxic effect of ditercalinium was suppressed by the uvrA mutation. DNA single strand breaks accumulated in...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.3.1063
更新日期：1988-02-11 00:00:00
abstract：:Large expansions of the trinucleotide repeat GAA*TTC within the first intron of the X25 (frataxin) gene cause Friedreich's ataxia, the most common inherited ataxia. Expansion leads to reduced levels of frataxin mRNA in affected individuals. Here we show that GAA*TTC tracts, in the absence of any other frataxin gene se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.14.2815
更新日期：2000-07-15 00:00:00
abstract：:Mechanisms that preserve genome integrity are highly important during the normal life cycle of human cells. Loss of genome protective mechanisms can lead to the development of diseases such as cancer. Checkpoint kinases function in the cellular surveillance pathways that help cells to cope with DNA damage. Importantly...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkr697
更新日期：2012-01-01 00:00:00
abstract：:A normal human population has been screened for the existence of further restriction fragment length polymorphisms (RFLPs) in the clotting factor IX gene in addition to the TaqI polymorphism already characterised (1,2). Two polymorphic loci were found, both within 6 Kb of the TaqI polymorphism within the body of the f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.23.8861
更新日期：1984-12-11 00:00:00
abstract：:A set of programs is presented for the reconstruction of a DNA sequence from data generated by the M13 shotgun sequencing technique. Once the sequence has been established and stored other programs are used for its analysis. The programs have been written for the Apple II microcomputer. A minimum investment is require...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.1.39
更新日期：1982-01-11 00:00:00
abstract：:High affinity antisense oligonucleotides (ASOs) containing bicylic modifications (BNA) such as locked nucleic acid (LNA) designed to induce target RNA cleavage have been shown to have enhanced potency along with a higher propensity to cause hepatotoxicity. In order to understand the mechanism of this hepatotoxicity, t...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1210
更新日期：2016-03-18 00:00:00
abstract：:SINE-VNTR-Alu (SVA) elements are non-autonomous, hominid-specific non-LTR retrotransposons and distinguished by their organization as composite mobile elements. They represent the evolutionarily youngest, currently active family of human non-LTR retrotransposons, and sporadically generate disease-causing insertions. S...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr863
更新日期：2012-02-01 00:00:00
abstract：:Deproteinated 16S RNA was iodinated at pH 5.0 in an aqueous solution containing TlCl3 plus KI for 1-5 hours at 42 degrees C. Under these conditions 33 moles of iodine are incorporated per mole of RNA. As judged by sucrose gradient sedimentation, the iodinated RNA does not exhibit any large alteration in conformation a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.11.3001
更新日期：1976-11-01 00:00:00
abstract：:A successful application is reported of the multivariate curve resolution alternating least-squares method (MCR-ALS) for the analysis of nucleic acid melting and salt-induced transitions. Under conditions where several structures co-exist in a conformational equilibrium, MCR-ALS analysis of the UV and circular dichroi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gnf091
更新日期：2002-09-01 00:00:00
abstract：:Mitochondrial-DNA diseases have no effective treatments. Allotopic expression-synthesis of a wild-type version of the mutated protein in the nuclear-cytosolic compartment and its importation into mitochondria-has been proposed as a gene-therapy approach. Allotopic expression has been successfully demonstrated in yeast...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq769
更新日期：2011-01-01 00:00:00
abstract：:The potent anticancer drug actinomycin D (ActD) functions by intercalating into DNA at GpC sites, thereby interrupting essential biological processes including replication and transcription. Certain neurological diseases are correlated with the expansion of (CGG)n trinucleotide sequences, which contain many contiguous...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt084
更新日期：2013-04-01 00:00:00
abstract：:Mutational analysis of the 5'-untranslated leader sequence (omega) of tobacco mosaic virus (TMV) was carried out to determine those sequences necessary for the translational enhancement associated with omega. Five deletion mutants, a single base substitution, and a 25 base replacement mutant were tested for alteration...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.3.883
更新日期：1988-02-11 00:00:00
abstract：:Restriction endonucleases are highly specific in recognizing the particular DNA sequence they act on. However, their activity is affected by sequence context, enzyme concentration and buffer composition. Changes in these factors may lead to either ineffective cleavage at the cognate restriction site or relaxed specifi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt257
更新日期：2013-06-01 00:00:00
abstract：:The alternative proliferating-cell nuclear antigen (PCNA)-loader CTF18-RFC forms a stable complex with DNA polymerase ε (Polε). We observed that, under near-physiological conditions, CTF18-RFC alone loaded PCNA inefficiently, but loaded it efficiently when complexed with Polε. During efficient PCNA loading, CTF18-RFC ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx096
更新日期：2017-05-05 00:00:00
abstract：:The Comparative Toxicogenomics Database (CTD; http://ctdbase.org/) is a premier public resource for literature-based, manually curated associations between chemicals, gene products, phenotypes, diseases, and environmental exposures. In this biennial update, we present our new chemical-phenotype module that codes chemi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky868
更新日期：2019-01-08 00:00:00
