Hepatotoxicity of high affinity gapmer antisense oligonucleotides is mediated by RNase H1 dependent promiscuous reduction of very long pre-mRNA transcripts.

abstract：:The noncoding RNA database (ncRNAdb) was created as a source of information on RNA molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to constitutively expressed, housekeeping or infrastructural RNAs, there is a wide variety of RNAs participating in mechanisms involved...

journal_title：Nucleic acids research

authors： Szymanski M,Erdmann VA,Barciszewski J

更新日期：2007-01-01 00:00:00

abstract：:The signal recognition particle database (SRPDB) is located at the University of Texas Health Science Center at Tyler and includes tabulations of SRP RNA, SRP protein and SRP receptor sequences. The sequences are annotated with links to the primary databases. They are ordered alphabetically or phylogenetically and are...

journal_title：Nucleic acids research

authors： Samuelsson T,Zwieb C

更新日期：1999-01-01 00:00:00

abstract：:Transcriptional repression and activation by nuclear receptors (NRs) are brought about by coregulator complexes. These complexes modify the chromatin environment of target genes and affect the activity of the basal transcription machinery. We have previously implicated the yeast ADA3 protein in transcriptional activat...

journal_title：Nucleic acids research

authors： Benecke A,Gaudon C,Garnier JM,vom Baur E,Chambon P,Losson R

更新日期：2002-06-01 00:00:00

abstract：:Heat shock genes are activated by the binding of the heat shock transcription factor (HSF) to heat shock elements (HSEs), consisting of arrays of the 5-bp unit NGAAN arranged as inverted repeats. Here, we have investigated the interaction of the 5-bp unit with HSFs of Drosophila and Saccharomyces. Mutations within the...

journal_title：Nucleic acids research

authors： Fernandes M,Xiao H,Lis JT

更新日期：1994-01-25 00:00:00

abstract：:Histone H2A ubiquitination plays critical roles in transcriptional repression and deoxyribonucleic acid (DNA) damage response. More attention has been focused on ubiquitin E3 ligases of H2A, however, less is known about the negative regulators of H2A ubiquitination. Here we identified HSCARG as a new negative regulato...

journal_title：Nucleic acids research

authors： Hu B,Li S,Zhang X,Zheng X

更新日期：2014-05-01 00:00:00

abstract：:Meiotic cells possess surveillance mechanisms that monitor critical events such as recombination and chromosome synapsis. Meiotic defects resulting from the absence of the synaptonemal complex component Zip1 activate a meiosis-specific checkpoint network resulting in delayed or arrested meiotic progression. Pch2 is an...

journal_title：Nucleic acids research

authors： Herruzo E,Ontoso D,González-Arranz S,Cavero S,Lechuga A,San-Segundo PA

更新日期：2016-09-19 00:00:00

abstract：:A peculiar phenomenon is observed in several adenovirus type 2 or 5 (Ad2 or Ad5) transformed cell lines: the right hand and left hand terminal regions of the viral genome present in the viral DNA insertions of these cell lines are found to be linked together. A large part of the viral DNA insertion present in the Ad5 ...

journal_title：Nucleic acids research

authors： Visser L,Reemst AC,van Mansfeld AD,Rozijn TH

更新日期：1982-04-10 00:00:00

abstract：:DNA polymerase III (DNA pol III) efficiently replicates the Escherichia coli genome, but it cannot bypass DNA damage. Instead, translesion synthesis (TLS) DNA polymerases are employed to replicate past damaged DNA; however, the exchange of replicative for TLS polymerases is not understood. The umuD gene products, whic...

journal_title：Nucleic acids research

authors： Silva MC,Nevin P,Ronayne EA,Beuning PJ

更新日期：2012-07-01 00:00:00

abstract：:Linkage analysis is a successful procedure to associate diseases with specific genomic regions. These regions are often large, containing hundreds of genes, which make experimental methods employed to identify the disease gene arduous and expensive. We present two methods to prioritize candidates for further experimen...

journal_title：Nucleic acids research

authors： George RA,Liu JY,Feng LL,Bryson-Richardson RJ,Fatkin D,Wouters MA

更新日期：2006-01-01 00:00:00

abstract：:Tunable induction of gene expression is an essential tool in biology and biotechnology. In spite of that, current induction systems often exhibit unpredictable behavior and performance shortcomings, including high sensitivity to transactivator dosage and plasmid take-up variation, and excessive consumption of cellular...

journal_title：Nucleic acids research

authors： Lillacci G,Benenson Y,Khammash M

更新日期：2018-10-12 00:00:00

abstract：:DNA dodecamers have been designed with two cytosines on each end and intervening A and T stretches, such that the oligomers have fully complementary A:T base pairs when aligned in the parallel orientation. Spectroscopic (UV, CD and IR), NMR and molecular dynamics studies have shown that oligomers having the sequences ...

journal_title：Nucleic acids research

authors： Parvathy VR,Bhaumik SR,Chary KV,Govil G,Liu K,Howard FB,Miles HT

更新日期：2002-04-01 00:00:00

abstract：:We have studied a novel class of DNA sequences that cause DNA polymerases to pause. These sequences have the central consensus Py-G-C and are not necessarily adjacent to hairpins in the DNA template. Since most consensus sequences do not cause pauses under standard conditions, additional template features must exist t...

journal_title：Nucleic acids research

authors： Mytelka DS,Chamberlin MJ

更新日期：1996-07-15 00:00:00

abstract：:Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studie...

journal_title：Nucleic acids research

authors： Newman JA,Savitsky P,Allerston CK,Bizard AH,Özer Ö,Sarlós K,Liu Y,Pardon E,Steyaert J,Hickson ID,Gileadi O

更新日期：2015-05-26 00:00:00

abstract：:8-Oxo-7,8,-dihydro-2'-deoxyguanosine triphosphate (8-oxo-dGTP) is a major product of oxidative damage in the nucleotide pool. It is capable of mispairing with adenosine (dA), resulting in futile, mutagenic cycles of base excision repair. Therefore, it is critical that DNA polymerases discriminate against 8-oxo-dGTP at...

journal_title：Nucleic acids research

authors： Burak MJ,Guja KE,Garcia-Diaz M

更新日期：2015-09-18 00:00:00

abstract：:LTR-IS elements are middle repetitive sequences in the mouse genome with structural features of solitary retroviral LTRs. In order to get some insight in the possible functional role of these sequences the promotor activity of two LTR-IS representatives differing by 105 bp in their U3 region was investigated. Gene fus...

journal_title：Nucleic acids research

authors： Köhrer K,Grummt I,Horak I

更新日期：1985-04-11 00:00:00

abstract：:Earlier we have shown that exogenous expression of HIPPI, a molecular partner of Huntingtin interacting protein HIP-1, induces apoptosis and increases expression of caspases-1, -8 and -10 in HeLa and Neuro2A cells. The C-terminal pseudo death effector domain of HIPPI (pDED-HIPPI) specifically interacts with the putati...

journal_title：Nucleic acids research

authors： Banerjee M,Datta M,Majumder P,Mukhopadhyay D,Bhattacharyya NP

更新日期：2010-01-01 00:00:00

abstract：:Comparative structural studies of ribosomes from various organisms keep offering exciting insights on how species-specific or environment-related structural features of ribosomes may impact translation specificity and its regulation. Although the importance of such features may be less obvious within more closely rela...

journal_title：Nucleic acids research

authors： Khusainov I,Vicens Q,Bochler A,Grosse F,Myasnikov A,Ménétret JF,Chicher J,Marzi S,Romby P,Yusupova G,Yusupov M,Hashem Y

更新日期：2016-12-01 00:00:00

abstract：:Discovering which regulatory proteins, especially transcription factors (TFs), are active under certain experimental conditions and identifying the corresponding binding motifs is essential for understanding the regulatory circuits that control cellular programs. The experimental methods used for this purpose are labo...

journal_title：Nucleic acids research

authors： Zamdborg L,Ma P

更新日期：2009-09-01 00:00:00

abstract：:Homologous recombination (HR) is important in repairing errors of replication and other forms of DNA damage. In mammalian cells, potential templates include the homologous chromosome, and after DNA replication, the sister chromatid. Previous work has shown that the mammalian recombination machinery is organized to sup...

journal_title：Nucleic acids research

authors： Read LR,Raynard SJ,Rukść A,Baker MD

更新日期：2004-02-20 00:00:00

abstract：:The speed of the new DNA sequencing techniques has created a need for computer programs to handle the data produced. This paper describes simple programs designed specifically for use by people with little or no computer experience. The programs are for use on small computers and provide facilities for storage, editin...

journal_title：Nucleic acids research

authors： Staden R

更新日期：1977-11-01 00:00:00

abstract：:Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in situ hybridization...

journal_title：Nucleic acids research

authors： Uno N,Kaku N,Morinaga Y,Hasegawa H,Yanagihara K

更新日期：2020-09-04 00:00:00

abstract：:A very mild and efficient procedure has been developed for the preparation of C-5 substituted deoxyuridines. The substituent carries a masked primary aliphatic amino group. These compounds are readily converted into their phosphoramidites and can be used to prepare oligonucleotides carrying one or more aliphatic amino...

journal_title：Nucleic acids research

authors： Haralambidis J,Chai M,Tregear GW

更新日期：1987-06-25 00:00:00

abstract：:Electrophoretic mobility shift assays (EMSA) were used to define the regions of the INO1 promoter that interact with factors present in extracts prepared from the yeast, Saccharomyces cerevisae. These experiments identified three different types of protein:DNA complexes that assemble with the INO1 promoter. Formation ...

journal_title：Nucleic acids research

authors： Lopes JM,Henry SA

更新日期：1991-07-25 00:00:00

abstract：:The major immediate early regulatory region of human cytomegalovirus (HCMV) has a complex set of DNA sites through which both cellular and viral factors coordinately regulate immediate early gene expression. In undifferentiated human teratocarcinoma (T2) cells we have previously shown that major immediate early gene e...

journal_title：Nucleic acids research

authors： Kothari S,Baillie J,Sissons JG,Sinclair JH

更新日期：1991-04-25 00:00:00

abstract：:Spliced leader (SL) trans-splicing is a critical element of gene expression in a number of eukaryotic groups. This process is arguably best understood in nematodes, where biochemical and molecular studies in Caenorhabditis elegans and Ascaris suum have identified key steps and factors involved. Despite this, the preci...

journal_title：Nucleic acids research

authors： Philippe L,Pandarakalam GC,Fasimoye R,Harrison N,Connolly B,Pettitt J,Müller B

更新日期：2017-08-21 00:00:00

abstract：:The -175 T greater than C mutation in the promoter of the A gamma- or G gamma-globin gene causes a 50-100 fold increase of the expression of the respective gene in adult erythroid cells (Hereditary Persistence of Fetal Hemoglobin). We show here that this mutation increases 3-9 fold the expression of a gamma-CAT report...

journal_title：Nucleic acids research

authors： Nicolis S,Ronchi A,Malgaretti N,Mantovani R,Giglioni B,Ottolenghi S

更新日期：1989-07-25 00:00:00

abstract：:Fluorogenic RNAs that are based on the complex formed by 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) derivatives and the RNA aptamer named Spinach were used to engineer a new generation of in vitro and in vivo sensors for bioanalytics. With the resolved crystal structure of the RNA/small molecule complex, ...

journal_title：Nucleic acids research

authors： Ketterer S,Fuchs D,Weber W,Meier M

更新日期：2015-10-30 00:00:00

abstract：:The cause of 50S ribosomal subunit collapse reportedly triggered by hybridization of a 14-base cDNA probe to the alpha-sarcin region of 23S rRNA was investigated by physical measurement of probe-subunit complexes in varying buffer conditions. The results reported here show that this probe was unable to hybridize to it...

journal_title：Nucleic acids research

authors： White GA,Wood T,Hill WE

更新日期：1988-11-25 00:00:00

abstract：:We previously showed that histone H4 serine-1 phosphorylation (H4S1ph) is evolutionarily conserved during gametogenesis, and contributes to post-meiotic nuclear compaction and to full completion of sporulation in the yeast Saccharomyces cerevisiae. Previous studies showed that H4S1ph and another modification of the sa...

journal_title：Nucleic acids research

authors： Govin J,Schug J,Krishnamoorthy T,Dorsey J,Khochbin S,Berger SL

更新日期：2010-08-01 00:00:00

abstract：:A synthetic gene for somatomedin C, a human growth factor, has been assembled by a single ligation of 23 oligodeoxyribonucleotides, which were chemically synthesized by an improved solid phase phosphotriester method. ...

journal_title：Nucleic acids research

authors： Sproat BS,Gait MJ

更新日期：1985-04-25 00:00:00