Abstract:
:Bloom's syndrome helicase (BLM) is a member of the RecQ family of DNA helicases, which play key roles in the maintenance of genome integrity in all organism groups. We describe crystal structures of the BLM helicase domain in complex with DNA and with an antibody fragment, as well as SAXS and domain association studies in solution. We show an unexpected nucleotide-dependent interaction of the core helicase domain with the conserved, poorly characterized HRDC domain. The BLM-DNA complex shows an unusual base-flipping mechanism with unique positioning of the DNA duplex relative to the helicase core domains. Comparison with other crystal structures of RecQ helicases permits the definition of structural transitions underlying ATP-driven helicase action, and the identification of a nucleotide-regulated tunnel that may play a role in interactions with complex DNA substrates.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Newman JA,Savitsky P,Allerston CK,Bizard AH,Özer Ö,Sarlós K,Liu Y,Pardon E,Steyaert J,Hickson ID,Gileadi Odoi
10.1093/nar/gkv373subject
Has Abstractpub_date
2015-05-26 00:00:00pages
5221-35issue
10eissn
0305-1048issn
1362-4962pii
gkv373journal_volume
43pub_type
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