当前位置： SCI文献检索 > NUCLEIC ACIDS RESEARCH期刊下所有文献 > The abundance of RNPS1, a protein component of the exon junction complex, can determine the variability in efficiency of the Nonsense Mediated Decay pathway.

The abundance of RNPS1, a protein component of the exon junction complex, can determine the variability in efficiency of the Nonsense Mediated Decay pathway.

Abstract：

:Nonsense-mediated mRNA decay (NMD) is a molecular pathway of mRNA surveillance that ensures rapid degradation of mRNAs containing premature translation termination codons (PTCs) in eukaryotes. NMD has been shown to also regulate normal gene expression and thus emerged as one of the key post-transcriptional mechanisms of gene regulation. Recently, NMD efficiency has been shown to vary between cell types and individuals thus implicating NMD as a modulator of genetic disease severity. We have now specifically analysed the molecular mechanism of variable NMD efficiency and first established an assay system for the quantification of NMD efficiency, which is based on carefully validated cellular NMD target transcripts. In a HeLa cell model system, NMD efficiency is shown to be remarkably variable and to represent a stable characteristic of different strains. In one of these strains, low NMD efficiency is shown to be functionally related to the reduced abundance of the exon junction component RNPS1. Furthermore, restoration of functional RNPS1 expression, but not of NMD-inactive mutant proteins, also restores efficient NMD in this model. We conclude that cellular concentrations of RNPS1 can modify NMD efficiency and propose that cell type specific co-factor availability represents a novel principle that controls NMD.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Viegas MH,Gehring NH,Breit S,Hentze MW,Kulozik AE

doi

10.1093/nar/gkm461

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

4542-51

issue

13

eissn

0305-1048

issn

1362-4962

pii

gkm461

journal_volume

35

pub_type

杂志文章

相关文献

NUCLEIC ACIDS RESEARCH文献大全
  • Imino proton NMR analysis of HDV ribozymes: nested double pseudoknot structure and Mg2+ ion-binding site close to the catalytic core in solution.

    abstract：:Minimized trans-acting HDV ribozyme systems consisting of three (Rz-3) and two (Rz-2) RNA strands were prepared and their folding conformations were analyzed by NMR spectroscopy. The guanosine residues in one of the enzyme components of Rz-3 were labeled with 13C and 15N. Imino proton signals were assigned by analysis...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/30.3.766

    authors： Tanaka Y,Hori T,Tagaya M,Sakamoto T,Kurihara Y,Katahira M,Uesugi S

    更新日期：2002-02-01 00:00:00

  • Direct interaction between Rsc6 and Rsc8/Swh3,two proteins that are conserved in SWI/SNF-related complexes.

    abstract：:The RSC complex of Saccharomyces cerevisiae is closely related to the SWI/SNF complex. Both complexes are involved in remodeling chromatin structure and they share conserved components. The RSC proteins Sth1, Rsc8/Swh3, Sfh1 and Rsc6 are homologs of the SWI/SNF proteins Swi2/Snf2, Swi3, Snf5 and Swp73 respectively. To...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/26.16.3739

    authors： Treich I,Ho L,Carlson M

    更新日期：1998-08-15 00:00:00

  • Amplification of genomic sequences flanking transposable elements in host and heterologous plants: a tool for transposon tagging and genome characterization.

    abstract：:The isolation of sequences flanking integrated transposable elements is an important step in gene tagging strategies. We have demonstrated that sequences flanking transposons integrated into complex genomes can be simply and rapidly obtained using the polymerase chain reaction. Amplification of such sequences was esta...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/18.11.3271

    authors： Earp DJ,Lowe B,Baker B

    更新日期：1990-06-11 00:00:00

  • Does the 'non-coding' strand code?

    abstract：:The hypothesis that DNA strands complementary to the coding strand contain in phase coding sequences has been investigated. Statistical analysis of the 50 genes of bacteriophage T7 shows no significant correlation between patterns of codon usage on the coding and non-coding strands. In Bacillus and yeast genes the cor...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/13.4.1389

    authors： Sharp PM

    更新日期：1985-02-25 00:00:00

  • Yeast two-hybrid junk sequences contain selected linear motifs.

    abstract：:Yeast two-hybrid (Y2H) screenings result in identification of many out-of-frame (OOF) clones that code for short (2-100 amino acids) peptides with no sequence homology to known proteins. We hypothesize that these peptides can reveal common short linear motifs (SLiMs) responsible for their selection. We present a new p...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr600

    authors： Liu Y,Woods NT,Kim D,Sweet M,Monteiro AN,Karchin R

    更新日期：2011-10-01 00:00:00

  • N-terminal phosphorylation of HP1α increases its nucleosome-binding specificity.

    abstract：:Heterochromatin protein 1 (HP1) is an evolutionarily conserved chromosomal protein that binds to lysine 9-methylated histone H3 (H3K9me), a hallmark of heterochromatin. Although HP1 phosphorylation has been described in several organisms, the biological implications of this modification remain largely elusive. Here we...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gku995

    authors： Nishibuchi G,Machida S,Osakabe A,Murakoshi H,Hiragami-Hamada K,Nakagawa R,Fischle W,Nishimura Y,Kurumizaka H,Tagami H,Nakayama J

    更新日期：2014-11-10 00:00:00

  • Distribution of topoisomerase II-mediated cleavage sites and relation to structural and functional landmarks in 830 kb of Drosophila DNA.

    abstract：:The pattern of sites for cleavage mediated by topoisomerase II was determined in 830 kb of cloned DNA from the Drosophila X chromosome, with the objectives of comparing it with mapped structural and functional landmarks and examining if the correlations with such landmarks reported in individual loci can be generalize...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/25.11.2041

    authors： Miassod R,Razin SV,Hancock R

    更新日期：1997-06-01 00:00:00

  • Single base extension (SBE) with proofreading polymerases and phosphorothioate primers: improved fidelity in single-substrate assays.

    abstract：:Model single base extension (SBE) genotyping reactions with individual deoxy-, dideoxy- and acyclonucleoside triphosphates are monitored by MALDI-TOF mass spectrometry. Three non-proofreading DNA polymerases display remarkably high misincorporation (up to 64% of correct incorporation) when extending primers with singl...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gng007

    authors： Di Giusto D,King GC

    更新日期：2003-02-01 00:00:00

  • Epigenetic changes in histone acetylation underpin resistance to the topoisomerase I inhibitor irinotecan.

    abstract：:The topoisomerase I (TOP1) inhibitor irinotecan triggers cell death by trapping TOP1 on DNA, generating cytotoxic protein-linked DNA breaks (PDBs). Despite its wide application in a variety of solid tumors, the mechanisms of cancer cell resistance to irinotecan remains poorly understood. Here, we generated colorectal ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkw1026

    authors： Meisenberg C,Ashour ME,El-Shafie L,Liao C,Hodgson A,Pilborough A,Khurram SA,Downs JA,Ward SE,El-Khamisy SF

    更新日期：2017-02-17 00:00:00

  • The conformational landscape of transcription intermediates involved in the regulation of the ZMP-sensing riboswitch from Thermosinus carboxydivorans.

    abstract：:Recently, prokaryotic riboswitches have been identified that regulate transcription in response to change of the concentration of secondary messengers. The ZMP (5-Aminoimidazole-4-carboxamide ribonucleotide (AICAR))-sensing riboswitch from Thermosinus carboxydivorans is a transcriptional ON-switch that is involved in ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa427

    authors： Binas O,Schamber T,Schwalbe H

    更新日期：2020-07-09 00:00:00

  • TFII-I-mediated polymerase pausing antagonizes GLI2 induction by TGFβ.

    abstract：:The modulation of GLI2, an oncogenic transcription factor commonly upregulated in cancer, is in many cases not due to genetic defects, suggesting dysregulation through alternative mechanisms. The identity of these molecular events remains for the most part unknown. Here, we identified TFII-I as a novel repressor of GL...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa476

    authors： McCleary-Wheeler AL,Paradise BD,Almada LL,Carlson AJ,Marks DL,Vrabel A,Vera RE,Sigafoos AN,Olson RL,Fernandez-Zapico ME

    更新日期：2020-07-27 00:00:00

  • Partitioning of zinc and copper within subnuclear nucleoprotein particles.

    abstract：:Nuclei from frozen calf thymus suspended in buffer were analyzed for metal content prior to and after repeated washing. After three such extractions about 0.1 micrograms Zn/mg DNA and 0.025 micrograms Cu/mg DNA remained tightly associated with chromatin. This level of metal was essentially unchanged with subsequent wa...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/9.21.5811

    authors： Bryan SE,Vizard DL,Beary DA,LaBiche RA,Hardy KJ

    更新日期：1981-11-11 00:00:00

  • EMAGE--Edinburgh Mouse Atlas of Gene Expression: 2008 update.

    abstract：:EMAGE (http://genex.hgu.mrc.ac.uk/Emage/database) is a database of in situ gene expression patterns in the developing mouse embryo. Domains of expression from raw data images are spatially integrated into a set of standard 3D virtual mouse embryos at different stages of development, allowing data interrogation by spat...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm938

    authors： Venkataraman S,Stevenson P,Yang Y,Richardson L,Burton N,Perry TP,Smith P,Baldock RA,Davidson DR,Christiansen JH

    更新日期：2008-01-01 00:00:00

  • Assembly, nuclear import and function of U7 snRNPs studied by microinjection of synthetic U7 RNA into Xenopus oocytes.

    abstract：:In Xenopus oocytes in vitro transcribed mouse U7 RNA is assembled into small nuclear ribonucleoproteins (snRNPs) that are functional in histone RNA 3' processing. If the special Sm binding site of U7 (AAUUUGUCUAG, U7 Sm WT) is converted into the canonical Sm sequence derived from the major snRNAs (AAUUUUUGGAG, U7 Sm O...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/23.16.3141

    authors： Stefanovic B,Hackl W,Lührmann R,Schümperli D

    更新日期：1995-08-25 00:00:00

  • Interactive Tree Of Life v2: online annotation and display of phylogenetic trees made easy.

    abstract：:Interactive Tree Of Life (http://itol.embl.de) is a web-based tool for the display, manipulation and annotation of phylogenetic trees. It is freely available and open to everyone. In addition to classical tree viewer functions, iTOL offers many novel ways of annotating trees with various additional data. Current versi...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr201

    authors： Letunic I,Bork P

    更新日期：2011-07-01 00:00:00

  • The effect of hybridization-induced secondary structure alterations on RNA detection using backscattering interferometry.

    abstract：:Backscattering interferometry (BSI) has been used to successfully monitor molecular interactions without labeling and with high sensitivity. These properties suggest that this approach might be useful for detecting biomarkers of infection. In this report, we identify interactions and characteristics of nucleic acid pr...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt165

    authors： Adams NM,Olmsted IR,Haselton FR,Bornhop DJ,Wright DW

    更新日期：2013-05-01 00:00:00

  • Determination of the molecular weight of DNA-bound protein(s) responsible for gel electrophoretic mobility shift of linear DNA fragments examplified with purified viral myb protein.

    abstract：:A protein-DNA complex has less gel electrophoretic mobility than the free DNA fragment. One parameter for the degree of retardation of a linear DNA fragment in a protein-DNA complex is the molecular weight of the bound protein(s). The quotient of the migration distances of free DNA (m) and protein-DNA complex (m') is ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/16.12.5241

    authors： Bading H

    更新日期：1988-06-24 00:00:00

  • Deaminase-independent inhibition of HIV-1 reverse transcription by APOBEC3G.

    abstract：:APOBEC3G (A3G), a host protein that inhibits HIV-1 reverse transcription and replication in the absence of Vif, displays cytidine deaminase and single-stranded (ss) nucleic acid binding activities. HIV-1 nucleocapsid protein (NC) also binds nucleic acids and has a unique property, nucleic acid chaperone activity, whic...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkm750

    authors： Iwatani Y,Chan DS,Wang F,Stewart-Maynard K,Sugiura W,Gronenborn AM,Rouzina I,Williams MC,Musier-Forsyth K,Levin JG

    更新日期：2007-01-01 00:00:00

  • Genome-wide prediction and analysis of human chromatin boundary elements.

    abstract：:Boundary elements partition eukaryotic chromatin into active and repressive domains, and can also block regulatory interactions between domains. Boundary elements act via diverse mechanisms making accurate feature-based computational predictions difficult. Therefore, we developed an unbiased algorithm that predicts th...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkr750

    authors： Wang J,Lunyak VV,Jordan IK

    更新日期：2012-01-01 00:00:00

  • Assessment of clone identity and sequence fidelity for 1189 IMAGE cDNA clones.

    abstract：:This report documents the error rate in a commercially distributed subset of the IMAGE Consortium mouse cDNA clone collection. After isolation of plasmid DNA from 1189 bacterial stock cultures, only 62. 2% were uncontaminated and contained cDNA inserts that had significant sequence identity to published data for the o...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/29.2.582

    authors： Halgren RG,Fielden MR,Fong CJ,Zacharewski TR

    更新日期：2001-01-15 00:00:00

  • ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin.

    abstract：:Zinc finger protein with KRAB and SCAN domains 3 (ZKSCAN3) has long been known as a master transcriptional repressor of autophagy. Here, we identify a novel role for ZKSCAN3 in alleviating senescence that is independent of its autophagy-related activity. Downregulation of ZKSCAN3 is observed in aged human mesenchymal ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkaa425

    authors： Hu H,Ji Q,Song M,Ren J,Liu Z,Wang Z,Liu X,Yan K,Hu J,Jing Y,Wang S,Zhang W,Liu GH,Qu J

    更新日期：2020-06-19 00:00:00

  • Alpha-amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes.

    abstract：:Many, if not all, mRNAs in T.brucei start with the same sequence of 35 nucleotides, separately encoded in clustered so-called mini-exon repeats. From these mini-exon repeats a 141-nt precursor RNA with the 35-nt sequence at its 5' end is transcribed. Indirect evidence suggests that this RNA is linked in a second step ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/12.24.9457

    authors： Kooter JM,Borst P

    更新日期：1984-12-21 00:00:00

  • DNA linking number change induced by sequence-specific DNA-binding proteins.

    abstract：:Sequence-specific DNA-binding proteins play a key role in many fundamental biological processes, such as transcription, DNA replication and recombination. Very often, these DNA-binding proteins introduce structural changes to the target DNA-binding sites including DNA bending, twisting or untwisting and wrapping, whic...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkq078

    authors： Chen B,Xiao Y,Liu C,Li C,Leng F

    更新日期：2010-06-01 00:00:00

  • Cyber-T web server: differential analysis of high-throughput data.

    abstract：:The Bayesian regularization method for high-throughput differential analysis, described in Baldi and Long (A Bayesian framework for the analysis of microarray expression data: regularized t-test and statistical inferences of gene changes. Bioinformatics 2001: 17: 509-519) and implemented in the Cyber-T web server, is ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks420

    authors： Kayala MA,Baldi P

    更新日期：2012-07-01 00:00:00

  • Identification and classification of bacterial Type III toxin-antitoxin systems encoded in chromosomal and plasmid genomes.

    abstract：:Toxin-antitoxin systems are widespread in bacteria and archaea. They perform diverse functional roles, including the generation of persistence, maintenance of genetic loci and resistance to bacteriophages through abortive infection. Toxin-antitoxin systems have been divided into three types, depending on the nature of...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gks231

    authors： Blower TR,Short FL,Rao F,Mizuguchi K,Pei XY,Fineran PC,Luisi BF,Salmond GP

    更新日期：2012-07-01 00:00:00

  • Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes.

    abstract：:Codon usage data has been compiled for 110 yeast genes. Cluster analysis on relative synonymous codon usage revealed two distinct groups of genes. One group corresponds to highly expressed genes, and has much more extreme synonymous codon preference. The pattern of codon usage observed is consistent with that expected...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/14.13.5125

    authors： Sharp PM,Tuohy TM,Mosurski KR

    更新日期：1986-07-11 00:00:00

  • CAMP: Collection of sequences and structures of antimicrobial peptides.

    abstract：:Antimicrobial peptides (AMPs) are gaining importance as anti-infective agents. Here we describe the updated Collection of Antimicrobial Peptide (CAMP) database, available online at http://www.camp.bicnirrh.res.in/. The 3D structures of peptides are known to influence antimicrobial activity. Although there exists datab...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkt1157

    authors： Waghu FH,Gopi L,Barai RS,Ramteke P,Nizami B,Idicula-Thomas S

    更新日期：2014-01-01 00:00:00

  • A non-canonical multisubunit RNA polymerase encoded by the AR9 phage recognizes the template strand of its uracil-containing promoters.

    abstract：:AR9 is a giant Bacillus subtilis phage whose uracil-containing double-stranded DNA genome encodes distant homologs of β and β' subunits of bacterial RNA polymerase (RNAP). The products of these genes are thought to assemble into two non-canonical multisubunit RNAPs - a virion RNAP (vRNAP) that is injected into the hos...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/gkx264

    authors： Sokolova M,Borukhov S,Lavysh D,Artamonova T,Khodorkovskii M,Severinov K

    更新日期：2017-06-02 00:00:00

  • Interactions between p53, hMSH2-hMSH6 and HMG I(Y) on Holliday junctions and bulged bases.

    abstract：:The ability of the tumor suppressor protein, p53, to recognize certain types of DNA lesions may represent one of the mechanisms by which this protein modulates cellular response to DNA damage. p53 DNA binding properties are regulated by several factors, such as post-translational modifications including phosphorylatio...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/30.11.2427

    authors： Subramanian D,Griffith JD

    更新日期：2002-06-01 00:00:00

  • Nucleotide sequence of the putative recognition site for coat protein in the RNAs of alfalfa mosaic virus and tobacco streak virus.

    abstract：:The sequence of the 3'-terminal 180 and 140 nucleotides of RNAs 2 and 3, respectively, of tobacco streak virus (TSV) was deduced by reverse transcription in the presence of a specific primer and chain terminators. Homology between the two RNAs was found to be restricted to a 3-terminal region of about 45 nucleotides. ...

    journal_title：Nucleic acids research

    pub_type： 杂志文章

    doi：10.1093/nar/8.15.3307

    authors： Koper-Zwarthoff EC,Bol JF

    更新日期：1980-08-11 00:00:00