Abstract:
:Cells deficient in the Werner syndrome protein (WRN) or BRCA1 are hypersensitive to DNA interstrand cross-links (ICLs), whose repair requires nucleotide excision repair (NER) and homologous recombination (HR). However, the roles of WRN and BRCA1 in the repair of DNA ICLs are not understood and the molecular mechanisms of ICL repair at the processing stage have not yet been established. This study demonstrates that WRN helicase activity, but not exonuclease activity, is required to process DNA ICLs in cells and that WRN cooperates with BRCA1 in the cellular response to DNA ICLs. BRCA1 interacts directly with WRN and stimulates WRN helicase and exonuclease activities in vitro. The interaction between WRN and BRCA1 increases in cells treated with DNA cross-linking agents. WRN binding to BRCA1 was mapped to BRCA1 452-1079 amino acids. The BRCA1/BARD1 complex also associates with WRN in vivo and stimulates WRN helicase activity on forked and Holliday junction substrates. These findings suggest that WRN and BRCA1 act in a coordinated manner to facilitate repair of DNA ICLs.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Cheng WH,Kusumoto R,Opresko PL,Sui X,Huang S,Nicolette ML,Paull TT,Campisi J,Seidman M,Bohr VAdoi
10.1093/nar/gkl362subject
Has Abstractpub_date
2006-05-19 00:00:00pages
2751-60issue
9eissn
0305-1048issn
1362-4962pii
34/9/2751journal_volume
34pub_type
杂志文章abstract::Ribosomal recruitment of cellular mRNAs depends on binding of eIF4F to the mRNA's 5'-terminal 'cap'. The minimal 'cap0' consists of N7-methylguanosine linked to the first nucleotide via a 5'-5' triphosphate (ppp) bridge. Cap0 is further modified by 2'-O-methylation of the next two riboses, yielding 'cap1' (m7GpppNmN) ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1321
更新日期:2014-03-01 00:00:00
abstract::On the tandemly linked ribosomal genes of Xenopus laevis, the RNA polymerase transcribes past the 3' end of the 40S coding region and terminates at T3 just upstream of the gene promoter. The close proximity of T3 to the gene promoter, and the functional interdependence of these two elements, has led to the suggestion ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/17.1.373
更新日期:1989-01-11 00:00:00
abstract::Histone H3K4 methylation is an epigenetic mark associated with actively transcribed genes. This modification is catalyzed by the mixed lineage leukaemia (MLL) family of histone methyltransferases including MLL1, MLL2, MLL3, MLL4, SET1A and SET1B. The catalytic activity of this family is dependent on interactions with ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkz697
更新日期:2019-09-26 00:00:00
abstract::Comparisons of proteins show that they evolve through the movement of domains. However, in many cases, the underlying mechanisms remain unclear. Here, we observed the movements of DNA recognition domains between non-orthologous proteins within a prokaryote genome. Restriction-modification (RM) systems, consisting of a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks681
更新日期:2012-10-01 00:00:00
abstract::Intramolecular RNA cross-links were induced within the large ribosomal subunit of E. coli by mild ultraviolet irradiation. Regions of the 23S RNA previously implicated in interactions with ribosomal-bound tRNA were then specifically excised by addressed cleavage using ribonuclease H, in conjunction with synthetic comp...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.15.4325
更新日期:1990-08-11 00:00:00
abstract::The transcription factor Sox5 has previously been shown in chicken to be expressed in early neural crest cells and neural crest-derived peripheral glia. Here, we show in mouse that Sox5 expression also continues after neural crest specification in the melanocyte lineage. Despite its continued expression, Sox5 has litt...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn527
更新日期:2008-10-01 00:00:00
abstract::The role of TATA elements in the expression of a mammalian promoter was investigated in the fission yeast Schizosaccharomyces pombe, by studying the human adenovirus E2-early promoter. This is a unique dual promoter with two nonconsensus TATA elements directing transcription from two cap sites, +1 and -26. A sequence ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.11.2737
更新日期:1993-06-11 00:00:00
abstract::Using a powerful computer-assisted analysis strategy, a large-scale search of small nucleolar RNA (snoRNA) genes in the recently released draft sequence of the rice genome was carried out. This analysis identified 120 different box C/D snoRNA genes with a total of 346 gene variants, which were predicted to guide 135 2...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg373
更新日期:2003-05-15 00:00:00
abstract::With the rapid accumulation of our knowledge on diseases, disease-related genes and drug targets, network-based analysis plays an increasingly important role in systems biology, systems pharmacology and translational science. The new release of VisANT aims to provide new functions to facilitate the convenient network ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt401
更新日期:2013-07-01 00:00:00
abstract::Genomes mutate and evolve in ways simple (substitution or deletion of bases) and complex (e.g. chromosome shattering). We do not fully understand what types of complex mutation occur, and we cannot routinely characterize arbitrarily-complex mutations in a high-throughput, genome-wide manner. Long-read DNA sequencing m...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx1266
更新日期:2018-02-28 00:00:00
abstract::The mitochondrial DNA (mtDNA) control regions for common chimpanzee, pygmy chimpanzee and gorilla were sequenced and the lengths and termini of their D-loop DNA's characterized. In these and all other species for which there are data, 5' termini map to sequences that contain the trinucleotide YAY. 3' termini are 25-51...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.13.5841
更新日期:1988-07-11 00:00:00
abstract::Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoure...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv311
更新日期:2015-05-19 00:00:00
abstract::The 3374 nucleotide sequence of RNA2 from the British PEBV strain SP5 has been determined. The RNA includes three open reading frames flanked by 5' and 3' noncoding regions of 509 and 480 nucleotides. The open reading frames specify coat protein, a 29.6K product homologous to the 29.1K product of TRV(TCM) RNA2 and a 2...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/18.15.4507
更新日期:1990-08-11 00:00:00
abstract::Work from a number of laboratories has indicated that the TATA box sequence can act as a basal promoter element not only for RNA polymerase II (RNAP II) transcription, but also for transcription by RNA polymerase III (RNAP III). We previously reported that, in the absence of other cis-acting elements, the canonical TA...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.15.3100
更新日期:1996-08-01 00:00:00
abstract::HT1080 cells stably expressing green fluorescent protein (GFP) linked to a 3' terminal AU-rich element (ARE) proved to be a convenient system to study the dynamics of mRNA stability, as changes in mRNA levels are reflected in increased or decreased fluorescence intensity. This study examined whether mRNA stability can...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkh282
更新日期:2004-02-19 00:00:00
abstract::The sigma(54) factor associates with core RNA polymerase (RNAP) to form a holoenzyme that is unable to initiate transcription unless acted on by an activator protein. sigma(54) is closely involved in many steps of activator-dependent transcription, such as core RNAP binding, promoter recognition, activator interaction...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp419
更新日期:2009-07-01 00:00:00
abstract::Inability of T7 RNA polymerase to processively transcribe higher eukaryotic chromatin is interpreted as a correlate of its reported inhibition by nucleosomes on reconstituted templates in vitro . We used chromosomally integrated reporter cassettes to examine features of T7 transcription in a lower eukaryotic system. L...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.20.4626
更新日期:1998-10-15 00:00:00
abstract::A mutant at the carboxyl end of the terminal protein, p3, of phage phi 29 DNA has been constructed by inserting an containing the stop translation codon TGA in the three possible reading frames, immediately downstream of a phage phi 29 DNA fragment coding for all but the last five amino acids of protein p3. The activ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/11.21.7397
更新日期:1983-11-11 00:00:00
abstract::The detailed conformational features and dynamics of the naturally occurring deoxyribotrinucleoside diphosphates d-TpTpT and d-TpTpC have been investigated at 20 degrees C and 80 degrees C in aqueous solution by nuclear magnetic resonance spectroscopy. The observed NMR parameters indicate that the conformational prope...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/5.11.4399
更新日期:1978-11-01 00:00:00
abstract::We have isolated and characterized DNA probes that detect homologies between the X and Y chromosomes. Clone St25 is derived from the q13-q22 region of the X chromosome and recognizes a 98% homologous sequence on the Y chromosome. Y specific fragments were present in DNAs from 5 Yq-individuals and from 4 out of 7 XX ma...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.15.5485
更新日期:1985-08-12 00:00:00
abstract::We have used computer assisted dot matrix and oligonucleotide frequency analyses to identify highly recurring sequence elements of 7-11 base pairs in eukaryotic genes and viral DNAs. Such elements are found much more frequently than expected, often with an average spacing of a few hundred base pairs. Furthermore, the ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.4.1835
更新日期:1987-02-25 00:00:00
abstract::Human MitBASE is a database collecting human mtDNA variants. This database is part of a greater mitochondrial genome database (MitBASE) funded within the EU Biotech Program. The present paper reports the recent improvements in data structure, data quality and data quantity. As far as the database structure is concerne...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.1.143
更新日期:1999-01-01 00:00:00
abstract::In order to study the intragenic profiles of active transcription, we determined the relative levels of active RNA polymerase II present at the 3'- and 5'-ends of 261 yeast genes by run-on. The results obtained indicate that the 3'/5' run-on ratio varies among the genes studied by over 12 log(2) units. This ratio seem...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkq215
更新日期:2010-08-01 00:00:00
abstract::Characterization of the ability of human interferons (IFNs) to rapidly induce genes led to the identification of the first two members of the STAT (signal transducers and activators of transcription) family, Stat1 and Stat2. To study the unique role of this transcription factor in IFN signaling under more physiologica...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.21.4191
更新日期:1999-11-01 00:00:00
abstract::Nucleotide excision DNA repair is mechanistically conserved across all kingdoms of life. In prokaryotes, this multi-enzyme process requires six proteins: UvrA-D, DNA polymerase I and DNA ligase. To examine how UvrC locates the UvrB-DNA pre-incision complex at a site of damage, we have labeled UvrB and UvrC with differ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt177
更新日期:2013-05-01 00:00:00
abstract::A straightforward enzymatic protocol for converting regular DNA into pseudo-complementary DNA could improve the performance of oligonucleotide microarrays by generating readily hybridizable structure-free targets. Here we screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn797
更新日期:2008-12-01 00:00:00
abstract::A vast amount of research on the regulation of gene expression has relied on plasmid reporter assays. In this study, we show that plasmids widely used for this purpose constitutively produce substantial amounts of RNA from a TATA-containing cryptic promoter within the origin of replication. Readthrough of these RNAs i...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gks451
更新日期:2012-08-01 00:00:00
abstract::Non-LTR retrotransposons are mobile genetic elements and play a major role in eukaryotic genome evolution and disease. Similar to retroviruses they encode a reverse transcriptase, but their genomic integration mechanism is fundamentally different, and they lack homologs of the retroviral nucleocapsid-forming protein G...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt786
更新日期:2013-12-01 00:00:00
abstract::In highly oncogenic adenovirus (Ad) 12-transformed cells, major histocompatibility complex (MHC) class I gene expression is down-regulated by the products of the viral E1A oncogene at the level of initiation of transcription. However, class I gene expression is unaltered or elevated in non-oncogenic Ad2- or Ad5-transf...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/22.22.4779
更新日期:1994-11-11 00:00:00
abstract::DNA polymerases of the A and B families, and reverse transcriptases, share a common mechanism for preventing incorporation of ribonucleotides: a highly conserved active site residue obstructing the position that would be occupied by a 2' hydroxyl group on the incoming nucleotide. In the family Y (lesion bypass) polyme...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg417
更新日期:2003-07-15 00:00:00