Abstract:
:We have used computer assisted dot matrix and oligonucleotide frequency analyses to identify highly recurring sequence elements of 7-11 base pairs in eukaryotic genes and viral DNAs. Such elements are found much more frequently than expected, often with an average spacing of a few hundred base pairs. Furthermore, the most abundant repetitive elements observed in the ovalbumin locus, the beta-globin gene cluster, the metallothionein gene and the viral genomes of SV40, polyoma, Herpes simplex-1 and Mouse Mammary Tumor Virus were sequences shown previously to be protein binding sites or sequences important for regulating gene expression. These sequences were present in both exons and introns as well as promoter regions. These observations suggest that such sequences are often highly overrepresented within the specific gene segments with which they are associated. Computer analysis of other genetic units, including viral genomes and oncogenes, has identified a number of highly recurring sequence elements that could serve similar regulatory or protein-binding functions. A model for the role of such reiterated sequence elements in DNA organization and function is presented.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Bodnar JW,Ward DCdoi
10.1093/nar/15.4.1835subject
Has Abstractpub_date
1987-02-25 00:00:00pages
1835-51issue
4eissn
0305-1048issn
1362-4962journal_volume
15pub_type
杂志文章abstract::We describe here the cloning, characterization and expression in E. coli of the gene coding for a DNA methylase from Spiroplasma sp. strain MQ1 (M.SssI). This enzyme methylates completely and exclusively CpG sequences. The Spiroplasma gene was transcribed in E. coli using its own promoter. Translation of the entire me...
journal_title:Nucleic acids research
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abstract::The cDNA of human DNA polymerase delta was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residue...
journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
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更新日期:2015-07-13 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.22.5067
更新日期:1993-11-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2012-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1986-03-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv666
更新日期:2015-09-18 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.7.1475
更新日期:1991-04-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:2014-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/24.8.1395
更新日期:1996-04-15 00:00:00
abstract::Not I linking clones contain sequences flanking Not I recognition sites and were previously shown to be tightly associated with CpG islands and genes. To directly assess the value of Not I clones in genome research, high density grids with 50 000 Not I linking clones originating from six representative Not I linking l...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.7.1635
更新日期:2000-04-01 00:00:00
abstract::Digestion of isolated Friend erythroleukemic cell nuclei with DNase I under conditions which selectively destroy the DNA of transcriptionally "active" genes releases into the supernatant fraction proteins of the non-histone "High Mobility Group" (HMGs). Two of these, HMG-14 and HMG-17(identified by solubility in trich...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/8.9.1947
更新日期:1980-05-10 00:00:00
abstract::Infrared Spectroscopy was used to determine the numbers of base pairs for yeast 5S RNA and 5.8S RNA. The spectra were recorded at 20 degrees C and 50 degrees C, where tertiary interactions are assumed to be of less importance. It may be concluded that the structure of both RNAs is highly ordered and that there are lar...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.15.3851
更新日期:1981-08-11 00:00:00
abstract::Isogenic pluripotent stem cells are critical tools for studying human neurological diseases by allowing one to study the effects of a mutation in a fixed genetic background. Of particular interest are the spectrum of autism disorders, some of which are monogenic such as Timothy syndrome (TS); others are multigenic suc...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv164
更新日期:2015-05-26 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/26.15.3494
更新日期:1998-08-01 00:00:00
abstract::The original cDNA sequence reported for the murine DNA methyltransferase (MTase) was not full length. Recently, additional cDNA sequences have been reported that lie upstream of the original and contain an extended open reading frame with three additional ATGs in frame with the coding region [Tucker et al . (1996) Pro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.22.4666
更新日期:1997-11-15 00:00:00
abstract::The family of ten-eleven translocation (Tet) dioxygenases is widely distributed across the eukaryotic tree of life, from mammals to the amoeboflagellate Naegleria gruberi. Like mammalian Tet proteins, the Naegleria Tet-like protein, NgTet1, acts on 5-methylcytosine (5mC) and generates 5-hydroxymethylcytosine (5hmC), 5...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv870
更新日期:2015-12-15 00:00:00
abstract::We have previously investigated the role of the N-terminal region of ribosomal protein S4 to participate in 30S ribosome assembly and function (1-3). In this report we extend these studies to the two fragments produced by the chemical cleavage of protein S4 at the tryptophan residue 167. We find that the carboxyl term...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.17.6343
更新日期:1985-09-11 00:00:00
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journal_title:Nucleic acids research
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更新日期:1996-03-15 00:00:00
abstract::The 'POU' (acronym of Pit-1, Oct-1, Unc-86) family of transcription factors share a common DNA-binding domain of approximately 160 residues, comprising so-called 'POUs' and 'POUh' sub-domains connected by a flexible linker. The importance of POU proteins as developmental regulators and tumor-promoting agents is due to...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Nucleic acids research
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更新日期:2013-02-01 00:00:00
abstract::The principles of mRNA decoding are conserved among all extant life forms. We present an integrative view of all the interaction networks between mRNA, tRNA and rRNA: the intrinsic stability of codon-anticodon duplex, the conformation of the anticodon hairpin, the presence of modified nucleotides, the occurrence of no...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkw608
更新日期:2016-09-30 00:00:00
abstract::It has recently been demonstrated that nucleobase-density profiles of typical mRNA coding sequences exhibit a complementary relationship with nucleobase-interaction propensity profiles of their cognate protein sequences. This finding supports the idea that the genetic code developed in response to direct binding inter...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv166
更新日期:2015-03-31 00:00:00
abstract::The possibility of cloning large segments of DNA in cosmid vectors offers distinct advantages, in particular for the study of multigene families. Large size fragments of mouse embryo DNA were successfully cloned in the cosmid pHC 79. Twelve recombinants hybridizing specifically to an immunoglobulin kappa chain variabl...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.12.2777
更新日期:1981-06-25 00:00:00
abstract::The CDC16 gene is involved in the process of chromosome segregation in mitosis and a cdc16ts mutant accumulates the predominant microtubule-associated protein at the nonpermissive temperature. We find that the CDC16 gene open reading frame (ORF) is capable of encoding a protein whose calculated molecular weight and pI...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/15.20.8439
更新日期:1987-10-26 00:00:00
abstract::The Mouse Genome Database (MGD; http://www.informatics.jax.org) is the primary community model organism database for the laboratory mouse and serves as the source for key biological reference data related to mouse genes, gene functions, phenotypes and disease models with a strong emphasis on the relationship of these ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkv1211
更新日期:2016-01-04 00:00:00
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journal_title:Nucleic acids research
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doi:10.1093/nar/gkl136
更新日期:2006-04-20 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/gkaa261
更新日期:2020-05-21 00:00:00
abstract::Thyroid hormone receptors (TRs) are members of the nuclear hormone receptor superfamily, which act as transcription factors upon binding to specific DNA sequences called thyroid hormone (T3) response elements (TREs). Such elements are found in the upstream regulatory region of promoters as well as in intragenic sequen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/25.9.1774
更新日期:1997-05-01 00:00:00