Abstract:
:The cDNA of human DNA polymerase delta was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residues specifically immunoblotted the human pol delta catalytic polypeptide. A multiple sequence alignment was constructed. This showed that human pol delta is closely related to yeast pol delta and the herpes virus DNA polymerases. The levels of pol delta message were found to be induced concomitantly with DNA pol delta activity and DNA synthesis in serum restimulated proliferating IMR90 cultured cells. The human pol delta gene was localized to chromosome 19 by Southern blotting of EcoRI digested DNA from a panel of rodent/human cell hybrids.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Yang CL,Chang LS,Zhang P,Hao H,Zhu L,Toomey NL,Lee MYdoi
10.1093/nar/20.4.735keywords:
subject
Has Abstractpub_date
1992-02-25 00:00:00pages
735-45issue
4eissn
0305-1048issn
1362-4962journal_volume
20pub_type
杂志文章abstract::To synchronize SV40 replicons, simian cells infected with a tsA mutant were restricted at 40 degrees, to complete ongoing replication and returned to 32 degrees, to activate new replicons in the presence of the DNA chain elongation inhibitor aphidicolin. Upon further incubation at 40 degrees without the drug, 3H-dT wa...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/10.2.763
更新日期:1982-01-22 00:00:00
abstract::The numerous genomic sequences and ESTs released by the Arabidopsis thaliana Genome Initiative (AGI) have allowed a systematic and functional study of the DEAD box RNA helicase family. Sequencing and in silico analysis led to the characterization of 28 novel A. thaliana DEAD box RNA helicases forming a family of 32 me...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/27.2.628
更新日期:1999-01-15 00:00:00
abstract::Various antitumor drugs stabilize DNA topoisomerase II-DNA transient covalent complexes. The complexes distribution along pBR322 DNA was shown previously to depend upon the nature of the drug (Tewey et al. (1984) Science 226, 466-468). The position in pBR322 of DNA cleavage by calf DNA topoisomerase II for 115 such si...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.11.2861
更新日期:1991-06-11 00:00:00
abstract::Ribosomal protein (RP) genes must be coordinately expressed for proper assembly of the ribosome yet the mechanisms that control expression of RP genes in metazoans are poorly understood. Recently, TATA-binding protein-related factor 2 (TRF2) rather than the TATA-binding protein (TBP) was found to function in transcrip...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx676
更新日期:2017-10-13 00:00:00
abstract::We have characterized triple-helix-mediated inhibition of an artificial bacteriophage promoter with respect to relief of inhibition by incoming RNA polymerases that initiate upstream or downstream from the operator sequence. Whereas oligonucleotide-directed triple-helix formation inhibits the test promoter, promoter a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/21.17.4055
更新日期:1993-08-25 00:00:00
abstract::DNA molecules with covalently sealed (hairpin) ends are probable intermediates in V(D)J recombination. According to current models hairpin ends are opened to produce short single-stranded extensions that are thought to be precursors of a particular type of extra nucleotides, termed P nucleotides, which are frequently ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/23.19.3872
更新日期:1995-10-11 00:00:00
abstract::The molecular structure of the DNA double helix has been known for 60 years, but we remain surprisingly ignorant of the balance of forces that determine its mechanical properties. The DNA double helix is among the stiffest of all biopolymers, but neither theory nor experiment has provided a coherent understanding of t...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt808
更新日期:2013-12-01 00:00:00
abstract::The main goal of this review is to provide some guide-lines for choosing a proper method when searching for differentially expressed transcripts. The choice of an approach should depend upon particular features of the experiment and project requirements. We outline some general considerations to help decide between di...
journal_title:Nucleic acids research
pub_type: 杂志文章,评审
doi:10.1093/nar/26.24.5537
更新日期:1998-12-15 00:00:00
abstract::Egr-1 is a murine zinc finger encoding cDNA whose expression is modulated by a variety of ligand-receptor interactions and is often coregulated with c-fos (1). This study reports the isolation of a mouse Egr-1 genomic clone, its intron-exon structure, and 935 bp of 5' flanking sequence. The gene spans about 3.8 kb and...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.18.8835
更新日期:1988-09-26 00:00:00
abstract::Owing to their great potentials in genetic code extension and the development of nucleic acid-based functional nanodevices, DNA duplexes containing HgII-mediated base pairs have been extensively studied during the past 60 years. However, structural basis underlying these base pairs remains poorly understood. Herein, w...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkw1296
更新日期:2017-03-17 00:00:00
abstract::Short repetitive DNA sequences are believed to be one of the primordial genetic elements that served as a source of complex large DNA found in the genome of modern organisms. However, the mechanism of its expansion (increase in repeat number) during the course of evolution is unclear. We demonstrate that the DNA polym...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/28.20.3999
更新日期:2000-10-15 00:00:00
abstract::The noncoding RNA database (ncRNAdb) was created as a source of information on RNA molecules, which do not possess protein-coding capacity. It is now widely accepted that, in addition to constitutively expressed, housekeeping or infrastructural RNAs, there is a wide variety of RNAs participating in mechanisms involved...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkl994
更新日期:2007-01-01 00:00:00
abstract::We have used fluorescein-11-dUTP in a nick-translation format to produce fluoresceinated human nucleic acid probes. After in situ hybridization of fluoresceinated DNAs to human metaphase chromosomes, the detection sensitivity was found to be 50-100 kb. The feasibility and the increase in detection sensitivity of micro...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/19.12.3237
更新日期:1991-06-25 00:00:00
abstract::Chromatin fragments released from intact Friend erythroleukemia cell nuclei during limited incubation with micrococcal nuclease, DNase II or DNase I were analyzed to determine the distribution of DNA methyltransferase in chromatin. The enzyme was released in a free form when internucleosomal DNA was digested with micr...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/9.20.5359
更新日期:1981-10-24 00:00:00
abstract::With an increased emphasis on genotyping of single nucleotide polymorphisms (SNPs) in disease association studies, the genotyping platform of choice is constantly evolving. In addition, the development of more specific SNP assays and appropriate genotype validation applications is becoming increasingly critical to elu...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gnh046
更新日期:2004-03-26 00:00:00
abstract::Benzo[c]phenanthrene dihydrodiol epoxide (B[c] PhDE) is well known as an important environmental chemical carcinogen that preferentially modifies DNA in adenine residues. However, the molecular mechanism by which B[c]PhDE induces tumorigenesis is not fully understood. In this report, we demonstrate that DNA mismatch r...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg875
更新日期:2003-11-15 00:00:00
abstract::The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast ce...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/20.8.1979
更新日期:1992-04-25 00:00:00
abstract::The RNA sequences boxA, boxB and boxC constitute the nut regions of phage lambda. They nucleate the formation of a termination-resistant RNA polymerase complex on the lambda chromosome. The complex includes E. coli proteins NusA, NusB, NusG and NusE, and the lambda N protein. A complex that includes the Nus proteins a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp452
更新日期:2009-08-01 00:00:00
abstract::DNA mismatches that occur between vector homology arms and chromosomal target sequences reduce gene targeting frequencies in several species; however, this has not been reported in human cells. Here we demonstrate that even a single mismatched base pair can significantly decrease human gene targeting frequencies. In a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkt1303
更新日期:2014-03-01 00:00:00
abstract::Combinatorial interactions of sequence-specific trans-acting factors with localized genomic cis-element clusters are the principal mechanism for regulating tissue-specific and developmental gene expression. With the emergence of expanding numbers of genome-wide expression analyses, the identification of the cis-elemen...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gki486
更新日期:2005-07-01 00:00:00
abstract::We have previously shown that Y box-binding protein-1 (YB-1) binds preferentially to cisplatin-modified Y box sequences. Based on structural and biochemical data, we predicted that this protein binds single-stranded nucleic acids. In the present study we confirmed the prediction and also discovered some unexpected fun...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.5.1200
更新日期:2001-03-01 00:00:00
abstract::The family Picornaviridae contains important pathogens including, for example, hepatitis A virus and foot-and-mouth disease virus. The genome of these viruses is a single messenger-active (+)-RNA of 7200-8500 nt. Besides coding for the viral proteins, it also contains functionally important RNA secondary structures, a...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/29.24.5079
更新日期:2001-12-15 00:00:00
abstract::Double-stranded soybean leghemoglobin DNA was synthesized from leghemoglobin mRNA isolated from soybean nodules. The dsDNA was inserted into the Bam H1 site of plasmid pBR322 using the poly-dAT-joiner method. A cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease di...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/6.9.3061
更新日期:1979-07-11 00:00:00
abstract::Here, we report a webserver for the improved SDM, used for predicting the effects of mutations on protein stability. As a pioneering knowledge-based approach, SDM has been highlighted as the most appropriate method to use in combination with many other approaches. We have updated the environment-specific amino-acid su...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkx439
更新日期:2017-07-03 00:00:00
abstract::Modification of cytosine plays an important role in epigenetic regulation of gene expression and genome stability. Cytosine is converted to 5-methylcytosine (5mC) by DNA methyltransferase; in turn, 5mC may be oxidized to 5-hydroxymethylcytosine (5hmC) by ten-eleven translocation enzyme. The structural flexibility of D...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkaa1210
更新日期:2021-01-25 00:00:00
abstract::Two DNA hexadecamers containing one central 5'-GC-3' base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from th...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/16.23.11125
更新日期:1988-12-09 00:00:00
abstract::The convergently transcribed restriction (R) and methylase (M) genes of the Restriction-Modification system Esp1396I are tightly regulated by a controller (C) protein that forms part of the CR operon. We have mapped the transcriptional start sites from each promoter and examined the regulatory role of C.Esp1396I in vi...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp210
更新日期:2009-06-01 00:00:00
abstract::Bacterial Hfq is a protein that plays an important role in the regulation of genes in cooperation with sRNAs. Escherichia coli Hfq (EcHfq) has two or more sites that bind RNA(s) including U-rich and/or the poly(A) tail of mRNA. However, functional and structural information about Bacillus subtilis Hfq (BsHfq) includin...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr892
更新日期:2012-02-01 00:00:00
abstract::Using a human interferon-alpha probe we have isolated recombinant phages containing murine interferon-alpha (Mu IFN-alpha) genes from a genomic library. One of these phages contained two complete Mu IFN-alpha genes and part of a third gene. The insert of a second phage held two IFN genes. This indicates that the Mu IF...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.3.791
更新日期:1985-02-11 00:00:00
abstract::The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution fol...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gku1349
更新日期:2015-01-01 00:00:00