Different strategies of differential display: areas of application.

abstract：:Oligoribonucleotides containing a 5'-phosphorothiolate linkage have provided effective tools to study the mechanisms of RNA catalysis, allowing resolution of kinetic ambiguity associated with mechanistic dissection and providing a strategy to establish linkage between catalysis and specific functional groups. However,...

journal_title：Nucleic acids research

authors： Li NS,Frederiksen JK,Koo SC,Lu J,Wilson TJ,Lilley DM,Piccirilli JA

更新日期：2011-03-01 00:00:00

abstract：:In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage lambda integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the parental enzyme,...

journal_title：Nucleic acids research

authors： Tay Y,Ho C,Droge P,Ghadessy FJ

更新日期：2010-03-01 00:00:00

abstract：:The cDNA of human DNA polymerase delta was cloned. The cDNA had a length of 3.5 kb and encoded a protein of 1107 amino acid residues with a calculated molecular mass of 124 kDa. Northern blot analysis showed that the cDNA hybridized to a mRNA of 3.4 kb. Monoclonal and polyclonal antibodies to the C-terminal 20 residue...

journal_title：Nucleic acids research

authors： Yang CL,Chang LS,Zhang P,Hao H,Zhu L,Toomey NL,Lee MY

更新日期：1992-02-25 00:00:00

abstract：:The Functional Single Nucleotide Polymorphism (F-SNP) database integrates information obtained from 16 bioinformatics tools and databases about the functional effects of SNPs. These effects are predicted and indicated at the splicing, transcriptional, translational and post-translational level. As such, the database h...

journal_title：Nucleic acids research

authors： Lee PH,Shatkay H

更新日期：2008-01-01 00:00:00

abstract：:The database of 3D interacting domains (3did) is a collection of protein interactions for which high-resolution 3D structures are known. 3did exploits structural information to provide the crucial molecular details necessary for understanding how protein interactions occur. Besides interactions between globular domain...

journal_title：Nucleic acids research

authors： Stein A,Panjkovich A,Aloy P

更新日期：2009-01-01 00:00:00

abstract：:I-motif DNAs have been widely employed as robust modulating components to construct reconfigurable DNA nanodevices that function well in acidic cellular environments. However, they generally display poor interactivity with fluorescent ligands under these complex conditions, illustrating a major difficulty in utilizing...

journal_title：Nucleic acids research

authors： Shi L,Peng P,Zheng J,Wang Q,Tian Z,Wang H,Li T

更新日期：2020-02-28 00:00:00

abstract：:Metagenomics is a relatively recently established but rapidly expanding field that uses high-throughput next-generation sequencing technologies to characterize the microbial communities inhabiting different ecosystems (including oceans, lakes, soil, tundra, plants and body sites). Metagenomics brings with it a number ...

journal_title：Nucleic acids research

authors： Hunter S,Corbett M,Denise H,Fraser M,Gonzalez-Beltran A,Hunter C,Jones P,Leinonen R,McAnulla C,Maguire E,Maslen J,Mitchell A,Nuka G,Oisel A,Pesseat S,Radhakrishnan R,Rocca-Serra P,Scheremetjew M,Sterk P,Vaughan D,

更新日期：2014-01-01 00:00:00

abstract：:Since July 1995, the European Bioinformatics Institute (EBI) has maintained RHdb (http://www.ebi.ac.uk/RHdb/RHdb.html ), a public database for radiation hybrid data. Radiation hybrid mapping is an important technique for determining high resolution maps. Recently, CORBA access has been added to Rhdb. The EBI is an Out...

journal_title：Nucleic acids research

authors： Lijnzaad P,Helgesen C,Rodriguez-Tomé P

更新日期：1998-01-01 00:00:00

abstract：:Many interacting proteins regulate and/or assist the activities of RAD51, a recombinase which plays a critical role in both DNA repair and meiotic recombination. Yeast two-hybrid screening of a human testis cDNA library revealed a new protein, RAD51AP2 (RAD51 Associated Protein 2), that interacts strongly with RAD51. ...

journal_title：Nucleic acids research

authors： Kovalenko OV,Wiese C,Schild D

更新日期：2006-01-01 00:00:00

abstract：:Glutaminyl-tRNA synthetase from Deinococcus radiodurans possesses a C-terminal extension of 215 residues appending the anticodon-binding domain. This domain constitutes a paralog of the Yqey protein present in various organisms and part of it is present in the C-terminal end of the GatB subunit of GatCAB, a partner of...

journal_title：Nucleic acids research

authors： Deniziak M,Sauter C,Becker HD,Paulus CA,Giegé R,Kern D

更新日期：2007-01-01 00:00:00

abstract：:We report that greA expression is driven by two strong, overlapping P1 and P2 promoters. The P1 promoter is sigma(70)-dependent and P2 is sigma(E)-dependent. Two-thirds of transcripts terminate within the leader region and the remaining third comprises greA mRNA. Termination efficiency seems to be unaffected by growth...

journal_title：Nucleic acids research

authors： Potrykus K,Murphy H,Chen X,Epstein JA,Cashel M

更新日期：2010-03-01 00:00:00

abstract：:We have previously shown that integration of a polyoma vector containing rodent repetitive elements into rat cellular DNA is non-random (Wallenburg et al. J. Virol. 50: 678-683). Junctions between the polyoma vector and the host DNA occur in the repetitive sequences of the vector about ten times more frequently than w...

journal_title：Nucleic acids research

authors： Wallenburg JC,Nepveu A,Chartrand P

更新日期：1987-10-12 00:00:00

abstract：:Recent genome-wide analyses have elucidated the extent of alternative splicing (AS) in mammals, often focusing on comparisons of splice isoforms between differentiated tissues. However, regulated splicing changes are likely to be important in biological transitions such as cellular differentiation, or response to envi...

journal_title：Nucleic acids research

authors： Bland CS,Wang ET,Vu A,David MP,Castle JC,Johnson JM,Burge CB,Cooper TA

更新日期：2010-11-01 00:00:00

abstract：:HinP1I recognizes and cleaves the palindromic tetranucleotide sequence G downward arrowCGC in DNA. We report three structures of HinP1I-DNA complexes: in the presence of Ca(2+) (pre-reactive complex), in the absence of metal ion (binary complex) and in the presence of Mg(2+) (post-reactive complex). HinP1I forms a bac...

journal_title：Nucleic acids research

authors： Horton JR,Zhang X,Maunus R,Yang Z,Wilson GG,Roberts RJ,Cheng X

更新日期：2006-02-09 00:00:00

abstract：:Understanding networks of protein-protein interactions constitutes an essential component on a path towards comprehensive description of cell function. Whereas efficient techniques are readily available for the initial identification of interacting protein partners, practical strategies are lacking for the subsequent ...

journal_title：Nucleic acids research

authors： Pajunen M,Turakainen H,Poussu E,Peränen J,Vihinen M,Savilahti H

更新日期：2007-01-01 00:00:00

abstract：:Transcription initiation complexes formed by bacterial RNA polymerases (RNAPs) exhibit dramatic species-specific differences in stability, leading to different strategies of transcription regulation. The molecular basis for this diversity is unclear. Promoter complexes formed by RNAP from Thermus aquaticus (Taq) are c...

journal_title：Nucleic acids research

authors： Mekler V,Minakhin L,Kuznedelov K,Mukhamedyarov D,Severinov K

更新日期：2012-12-01 00:00:00

abstract：:The linking number of DNA in minichromosomes increases by 2 turns during SV40 assembly. Changes in temperature also influence the average linking number of the total intracellular forms of SV40 DNA. When the isolated minichromosomes assembled in vivo are incubated with topoisomerase I at 33 degrees C in vitro, the lin...

journal_title：Nucleic acids research

authors： Ambrose C,McLaughlin R,Bina M

更新日期：1987-05-11 00:00:00

abstract：:Two hexamer DNA fragments containing a carcinogenic modified base, O6-methyl guanine, have been synthesized by a solid-phase phosphotriester method, in which the unmodified guanine residues present were O6 protected with the 4-nitrophenylethyl group. These two alkylated oligonucleotides were found to have similar Tm's...

journal_title：Nucleic acids research

authors： Kuzmich S,Marky LA,Jones RA

更新日期：1983-05-25 00:00:00

abstract：:Pyrrole-imidazole polyamides targeted to the androgen response element were cytotoxic in multiple cell lines, independent of intact androgen receptor signaling. Polyamide treatment induced accumulation of S-phase cells and of PCNA replication/repair foci. Activation of a cell cycle checkpoint response was evidenced by...

journal_title：Nucleic acids research

authors： Martínez TF,Phillips JW,Karanja KK,Polaczek P,Wang CM,Li BC,Campbell JL,Dervan PB

更新日期：2014-10-01 00:00:00

abstract：:Mammalian antibody switch regions (∼1500 bp) are composed of a series of closely neighboring G4-capable sequences. Whereas numerous structural and genome-wide analyses of roles for minimal G4s in transcriptional regulation have been reported, Long G4-capable regions (LG4s)-like those at antibody switch regions-remain ...

journal_title：Nucleic acids research

authors： Williams JD,Houserova D,Johnson BR,Dyniewski B,Berroyer A,French H,Barchie AA,Bilbrey DD,Demeis JD,Ghee KR,Hughes AG,Kreitz NW,McInnis CH,Pudner SC,Reeves MN,Stahly AN,Turcu A,Watters BC,Daly GT,Langley RJ,Gillesp

更新日期：2020-06-19 00:00:00

abstract：:A positive cis-acting DNA element in the near 5'-upstream region of the CYP2B1/B2 genes in rat liver was found to play an important role in the transcription of these genes. An oligonucleotide covering -69 to -98 nt mimicked the gel mobility shift pattern given by the fragment -179 to +29 nt, which was earlier found a...

journal_title：Nucleic acids research

authors： Upadhya P,Rao MV,Venkateswar V,Rangarajan PN,Padmanaban G

更新日期：1992-02-11 00:00:00

abstract：:Locked nucleic acid (LNA) is a chemically modified nucleic acid with its sugar ring locked in an RNA-like (C3'-endo) conformation. LNAs show extraordinary thermal stabilities when hybridized with DNA, RNA or LNA itself. We performed molecular dynamics simulations on five isosequential duplexes (LNA-DNA, LNA-LNA, LNA-R...

journal_title：Nucleic acids research

authors： Pande V,Nilsson L

更新日期：2008-03-01 00:00:00

abstract：:MethBank (http://bigd.big.ac.cn/methbank) is a database that integrates high-quality DNA methylomes across a variety of species and provides an interactive browser for visualization of methylation data. Here, we present an updated implementation of MethBank (version 3.0) by incorporating more DNA methylomes from multi...

journal_title：Nucleic acids research

authors： Li R,Liang F,Li M,Zou D,Sun S,Zhao Y,Zhao W,Bao Y,Xiao J,Zhang Z

更新日期：2018-01-04 00:00:00

abstract：:N-nitrosation of glycine and its derivatives generates potent alkylating agents that can lead to the formation of O(6)-carboxymethylguanine (O(6)-CMG) in DNA. O(6)-CMG has been identified in DNA derived from human colon tissue, and its occurrence has been linked to diets high in red and processed meats. By analogy to ...

journal_title：Nucleic acids research

authors： Zhang F,Tsunoda M,Suzuki K,Kikuchi Y,Wilkinson O,Millington CL,Margison GP,Williams DM,Czarina Morishita E,Takénaka A

更新日期：2013-05-01 00:00:00

abstract：:UHRF1 plays multiple roles in regulating DNMT1-mediated DNA methylation maintenance during DNA replication. The UHRF1 C-terminal RING finger functions as an ubiquitin E3 ligase to establish histone H3 ubiquitination at Lys18 and/or Lys23, which is subsequently recognized by DNMT1 to promote its localization onto repli...

journal_title：Nucleic acids research

authors： Li T,Wang L,Du Y,Xie S,Yang X,Lian F,Zhou Z,Qian C

更新日期：2018-04-06 00:00:00

abstract：:Human transcription factor IIIC contains a 55 kDa polypeptide which specifically interacts with the Adenovirus 2 VAI gene promoter and which mimics most of the DNA binding properties of the entire factor. The specificity and affinity of this protein:DNA interaction was demonstrated by: (i) Separation of purified fract...

journal_title：Nucleic acids research

authors： Schneider HR,Waldschmidt R,Seifart KH

更新日期：1990-08-25 00:00:00

abstract：:Ribosome biogenesis in Saccharomyces cerevisiae involves a regulon of >200 genes (Ribi genes) coordinately regulated in response to nutrient availability and cellular growth rate. Two cis-acting elements called PAC and RRPE are known to mediate Ribi gene repression in response to nutritional downshift. Here, we show t...

journal_title：Nucleic acids research

authors： Bosio MC,Fermi B,Spagnoli G,Levati E,Rubbi L,Ferrari R,Pellegrini M,Dieci G

更新日期：2017-05-05 00:00:00

abstract：:The RDM1 gene encodes a RNA recognition motif (RRM)-containing protein involved in the cellular response to the anti-cancer drug cisplatin in vertebrates. We previously reported a cDNA encoding the full-length human RDM1 protein. Here, we describe the identification of 11 human cDNAs encoding RDM1 protein isoforms. Th...

journal_title：Nucleic acids research

authors： Messaoudi L,Yang YG,Kinomura A,Stavreva DA,Yan G,Bortolin-Cavaillé ML,Arakawa H,Buerstedde JM,Hainaut P,Cavaillé J,Takata M,Van Dyck E

更新日期：2007-01-01 00:00:00

abstract：:CTCF plays a pivotal role in mediating chromatin interactions, but it does not do so alone. A number of factors have been reported to co-localize with CTCF and regulate CTCF loops, but no comprehensive analysis of binding partners has been performed. This prompted us to identify CTCF loop participants and regulators b...

journal_title：Nucleic acids research

authors： Hu G,Dong X,Gong S,Song Y,Hutchins AP,Yao H

更新日期：2020-09-25 00:00:00

abstract：:Error-free lesion bypass and error-prone lesion bypass are important cellular responses to DNA damage during replication, both of which require a DNA polymerase (Pol). To identify lesion bypass DNA polymerases, we have purified human Polkappa encoded by the DINB1 gene and examined its response to damaged DNA templates...

journal_title：Nucleic acids research

authors： Zhang Y,Yuan F,Wu X,Wang M,Rechkoblit O,Taylor JS,Geacintov NE,Wang Z

更新日期：2000-11-01 00:00:00