Identification and functional characterization of a cis-acting positive DNA element regulating CYP 2B1/B2 gene transcription in rat liver.

abstract：:The base pairing properties of oligonucleotide duplexes containing 8-aza-7-deaza-2'-deoxyisoguanosine, its 7-bromo or its 7-iodo derivative are described. The nucleosides were synthesized on a convergent route, protected and converted into phosphoramidites. Oligonucleotides were prepared on a solid-phase and were hybr...

journal_title：Nucleic acids research

authors： Seela F,Kröschel R

更新日期：2003-12-15 00:00:00

abstract：:Long-range enhancers of transcription are a key component of the genomic regulatory architecture. Recent studies have identified bi-directionally transcribed RNAs emanating from these enhancers known as eRNAs. However, it remains unclear how tightly coupled eRNA production is with enhancer activity. Through our system...

journal_title：Nucleic acids research

authors： Banerjee AR,Kim YJ,Kim TH

更新日期：2014-11-10 00:00:00

abstract：:Transcriptional repression of pathogen defense-related genes is essential for plant growth and development. Several proteins are known to be involved in the transcriptional regulation of plant defense responses. However, mechanisms by which expression of defense-related genes are regulated by repressor proteins are po...

journal_title：Nucleic acids research

authors： Kim HS,Park HC,Kim KE,Jung MS,Han HJ,Kim SH,Kwon YS,Bahk S,An J,Bae DW,Yun DJ,Kwak SS,Chung WS

更新日期：2012-10-01 00:00:00

abstract：:A computer program, BRUCE, was developed for the identification of transfer-messenger RNA (tmRNA) genes. The program employs heuristic algorithms to search for a tRNA(Ala)-like secondary structure surrounding a short sequence encoding the tag peptide. In the 57 completely sequenced bacterial genomes where tmRNA genes ...

journal_title：Nucleic acids research

authors： Laslett D,Canback B,Andersson S

更新日期：2002-08-01 00:00:00

abstract：:It has been proposed that phage lambda site-specific recombination proceeds via two independent strand exchanges: the first exchange forming a Holliday-structure which is then converted into complete recombinant products by the second strand exchange. If this hypothesis is correct, one should be able to trap the putat...

journal_title：Nucleic acids research

authors： Kitts PA,Nash HA

更新日期：1988-07-25 00:00:00

abstract：:Human somatic cells have essentially no telomerase activity. Telomerase is linked to tumor genesis and is a valuable marker for malignant growth. Extreme paucity of the enzyme neccessitated development of a PCR-based assay, 'telomeric repeat amplification protocol' (TRAP). Unfortunately, this method is not without dif...

journal_title：Nucleic acids research

authors： Krupp G,Kühne K,Tamm S,Klapper W,Heidorn K,Rott A,Parwaresch R

更新日期：1997-02-15 00:00:00

abstract：:Alternative splicing of mRNA precursors results in multiple protein variants from a single gene and is critical for diverse cellular processes and development. Xist encodes a long noncoding RNA which is a central player to induce X-chromosome inactivation in female mammals and has two major splicing variants: long and...

journal_title：Nucleic acids research

authors： Yue M,Ogawa Y

更新日期：2018-03-16 00:00:00

abstract：:Mixed-lineage leukemia (MLL) maintains the expression of cellular memory genes during development, while leukemic MLL fusion proteins aberrantly maintain expression of hematopoietic stem cell program genes such as HOXA9 to cause leukemia. However, the molecular mechanism of gene activation is unclear. Here we show tha...

journal_title：Nucleic acids research

authors： Okuda H,Kawaguchi M,Kanai A,Matsui H,Kawamura T,Inaba T,Kitabayashi I,Yokoyama A

更新日期：2014-04-01 00:00:00

abstract：:DNA lengths in the structural repeat units of Chinese hamster ovary (CHO) and chicken erythrocyte chromatin were compared by analyzing the sizes of DNA fragments produced after treatment of nuclei with staphylococcal nuclease. The repeat length of CHO chromatin (173 +- 4 BP) is about 20 base pairs (BP) smaller than th...

journal_title：Nucleic acids research

authors： Rill RL,Nelson DA,Oosterhof DK,Hozier JC

更新日期：1977-04-01 00:00:00

abstract：:From gene expression to nanotechnology, understanding and controlling DNA requires a detailed knowledge of its higher order structure and dynamics. Here we take advantage of the environment-sensitive photoisomerization of cyanine dyes to probe local and global changes in DNA structure. We report that a covalently atta...

journal_title：Nucleic acids research

authors： Morten MJ,Lopez SG,Steinmark IE,Rafferty A,Magennis SW

更新日期：2018-11-30 00:00:00

abstract：:The TcSNP database (http://snps.tcruzi.org) integrates information on genetic variation (polymorphisms and mutations) for different stocks, strains and isolates of Trypanosoma cruzi, the causative agent of Chagas disease. The database incorporates sequences (genes from the T. cruzi reference genome, mRNAs, ESTs and ge...

journal_title：Nucleic acids research

authors： Ackermann AA,Carmona SJ,Agüero F

更新日期：2009-01-01 00:00:00

abstract：:OligoArrayDb is a comprehensive database containing pangenomic oligonucleotide microarray probe sets designed for most of the sequenced genomes that are not covered by commercial catalog arrays. The availability of probe sequences, associated with custom microarray fabrication services offered by many companies and co...

journal_title：Nucleic acids research

authors： Rouillard JM,Gulari E

更新日期：2009-01-01 00:00:00

abstract：:Humans have two nearly identical copies of the survival motor neuron (SMN ) gene, SMN1 and SMN2. Homozygous loss of SMN1 causes spinal muscular atrophy (SMA). SMN2 is unable to prevent the disease due to skipping of exon 7. Using a systematic approach of in vivo selection, we have previously demonstrated that a weak 5...

journal_title：Nucleic acids research

authors： Singh NN,Singh RN,Androphy EJ

更新日期：2007-01-01 00:00:00

abstract：:Methylation of miRNAs at the 2'-hydroxyl group on the ribose at 3'-end (2'-O-methylation, 2'Ome) is critical for miRNA function in plants and Drosophila. Whether this methylation phenomenon exists for mammalian miRNA remains unknown. Through LC-MS/MS analysis, we discover that majority of miR-21-5p isolated from human...

journal_title：Nucleic acids research

authors： Liang H,Jiao Z,Rong W,Qu S,Liao Z,Sun X,Wei Y,Zhao Q,Wang J,Liu Y,Chen X,Wang T,Zhang CY,Zen K

更新日期：2020-07-27 00:00:00

abstract：:YdiV is a negative regulator of cell motility. It interacts with FlhD(4)C(2) complex, a product of flagellar master operon, which works as the transcription activator of all other flagellar operons. Here, we report the crystal structures of YdiV and YdiV(2)-FlhD(2) complex at 1.9 Å and 2.9 Å resolutions, respectively....

journal_title：Nucleic acids research

authors： Li B,Li N,Wang F,Guo L,Huang Y,Liu X,Wei T,Zhu D,Liu C,Pan H,Xu S,Wang HW,Gu L

更新日期：2012-11-01 00:00:00

abstract：:A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing S...

journal_title：Nucleic acids research

authors： Pyle LE,Finch LR

更新日期：1988-07-11 00:00:00

abstract：:In Saccharomyces cerevisiae, the DNA damage response (DDR) is activated by the spatio-temporal colocalization of Mec1-Ddc2 kinase and the 9-1-1 clamp. In the absence of direct means to monitor Mec1 kinase activation in vivo, activation of the checkpoint kinase Rad53 has been taken as a proxy for DDR activation. Here, ...

journal_title：Nucleic acids research

authors： Janke R,Herzberg K,Rolfsmeier M,Mar J,Bashkirov VI,Haghnazari E,Cantin G,Yates JR 3rd,Heyer WD

更新日期：2010-04-01 00:00:00

abstract：:In the haploid genome of Xenopus laevis there are two genes coding for the r-protein L14. It is not known if they are located on the same chromosome. cDNA clones deriving from the transcripts of the two genes have been isolated from an oocyte messenger cDNA bank showing that they are both expressed. We have studied th...

journal_title：Nucleic acids research

authors： Beccari E,Mazzetti P,Mileo A,Bozzoni I,Pierandrei-Amaldi P,Amaldi F

更新日期：1986-10-10 00:00:00

abstract：:Sas2p is a histone acetyltransferase implicated in the regulation of transcriptional silencing, and ORC is the six-subunit origin recognition complex involved in the initiation of DNA replication and the establishment of transcriptionally silent chromatin by silencers in yeast. We show here that SAS2 deletion (sas2Del...

journal_title：Nucleic acids research

authors： Zou Y,Bi X

更新日期：2008-09-01 00:00:00

abstract：:Dinucleoside diphosphates of the general type pGpN have been prepared enzymatically using ribonuclease N1. Alkylated uridines or cytidines, which are products of carcinogens acting on nucleic acids, were tested in dinucleoside diphosphates for their ability to stimulate the binding of Ala- or Val-tRNA to ribosomes. O2...

journal_title：Nucleic acids research

authors： Singer B,Pergolizzi RG,Grunberger D

更新日期：1979-04-01 00:00:00

abstract：:The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using (32)P-labeled material from T4-infected cultures of Escherichiacoli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAApsiCUGAUGAUGUGAGTpsiCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the class...

journal_title：Nucleic acids research

authors： Stahl S,Paddock GV,Abelson J

更新日期：1974-10-01 00:00:00

abstract：:Most proteins from higher organisms are known to be multi-domain proteins and contain substantial numbers of intrinsically disordered (ID) regions. To analyse such protein sequences, those from human for instance, we developed a special protein-structure-prediction pipeline and accumulated the products in the Structur...

journal_title：Nucleic acids research

authors： Motono C,Nakata J,Koike R,Shimizu K,Shirota M,Amemiya T,Tomii K,Nagano N,Sakaya N,Misoo K,Sato M,Kidera A,Hiroaki H,Shirai T,Kinoshita K,Noguchi T,Ota M

更新日期：2011-01-01 00:00:00

abstract：:A DNA region of 2750 base pairs encompassing the genes III, VI and I of bacteriophage M13 has been sequenced by the Maxam-Gilbert procedure. By establishing the nucleotide changes introduced by several amber mutations, the coding region and the regulatory signals of each gene have been deduced. The genes appear to spa...

journal_title：Nucleic acids research

authors： van Wezenbeek P,Schoenmakers JG

更新日期：1979-06-25 00:00:00

abstract：:The Homeodomain Resource is a comprehensive collection of sequence, structure and genomic information on the homeodomain protein family. Available through the Resource are both full-length and domain-only sequence data, as well as X-ray and NMR structural data for proteins and protein-DNA complexes. Also available is ...

journal_title：Nucleic acids research

authors： Banerjee-Basu S,Ferlanti ES,Ryan JF,Baxevanis AD

更新日期：1999-01-01 00:00:00

abstract：:Myocyte enhancer binding factor 2 (MEF2) proteins belong to the MADS box family of transcription factors and four MEF2 proteins, MEF2A, MEF2B, MEF2C and MEF2D, have been found. MEF2 proteins have been shown to play critical roles in differentiation of muscles and neuronal tissues. How transactivational activity of MEF...

journal_title：Nucleic acids research

authors： Choi SJ,Park SY,Han TH

更新日期：2001-07-01 00:00:00

abstract：:The higher order structure of the functionally important 530 loop in Escherichia coli 16S rRNA was studied in mutants with single base changes at position 517, which significantly impair translational fidelity. The 530 loop has been proposed to interact with the EF-Tu-GTP-aatRNA ternary complex during decoding. The re...

journal_title：Nucleic acids research

authors： Van Ryk DI,Dahlberg AE

更新日期：1995-09-11 00:00:00

abstract：:Reactive oxygen species (ROS) have been implicated as the cause of cumulative damage to DNA, proteins and lipids that can ultimately result in cell death. A common problem when measuring oxidative DNA damage has been the introduction of modifications in the native state of the molecule by many DNA isolation methods. W...

journal_title：Nucleic acids research

authors： Milano J,Day BJ

更新日期：2000-02-15 00:00:00

abstract：:Many mutations occur as a result of DNA synthesis past the site of DNA damage by DNA damage bypass polymerases. The frequency and types of mutations not only depend on the nature of the damage, but also on the sequence context, as revealed from analysis of mutation spectra of DNA exposed to mutagens. Herein we report ...

journal_title：Nucleic acids research

authors： Fang H,Taylor JS

更新日期：2008-10-01 00:00:00

abstract：:The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains sev...

journal_title：Nucleic acids research

authors： Ledeboer AM,Edens L,Maat J,Visser C,Bos JW,Verrips CT,Janowicz Z,Eckart M,Roggenkamp R,Hollenberg CP

更新日期：1985-05-10 00:00:00

abstract：:Human Hepatitis B Virus (HBV) RNAs contain a cis -acting sequence, the post-transcriptional regulatory element (HPRE), which facilitates the cytoplasmic localization of intronless transcripts. Our previous studies have shown that the HPRE is composed of at least two independent sub-elements, HPREalpha and HPREbeta, wh...

journal_title：Nucleic acids research

authors： Smith GJ 3rd,Donello JE,Lück R,Steger G,Hope TJ

更新日期：1998-11-01 00:00:00