A truncated DNA-damage-signaling response is activated after DSB formation in the G1 phase of Saccharomyces cerevisiae.

abstract：:Methyl carbon-13 NMR spectra of purified tRNA species are presented for the first time. In addition, these spectra of tRNA species specific for phenylalanine, tyrosine, and cysteine exhibited the first resolution of single methyl carbon resonances. Carbon-13 enriched methyl groups of ribothymidine (T) and 7-methylguan...

journal_title：Nucleic acids research

authors： Agris PF,Schmidt PG

更新日期：1980-05-10 00:00:00

abstract：:The E.coli ada gene protein coding region has been ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase gene promoter region to produce pADH06C. The yeast strains SX46A, 7799-4B and VV-6 are deficient in endogenous O6-alkylguanine-DNA-alkyltransferase and...

journal_title：Nucleic acids research

authors： Brozmanova J,Kleibl K,Vlckova V,Skorvaga M,Cernakova L,Margison GP

更新日期：1990-01-25 00:00:00

abstract：:The multifunctional HCV core protein consists of a hydrophilic RNA interacting D1 domain and a hydrophobic D2 domain interacting with membranes and lipid droplets. The core D1 domain was found to possess nucleic acid annealing and strand transfer properties. To further understand these chaperone properties, we investi...

journal_title：Nucleic acids research

authors： Sharma Kk,Didier P,Darlix JL,de Rocquigny H,Bensikaddour H,Lavergne JP,Pénin F,Lessinger JM,Mély Y

更新日期：2010-06-01 00:00:00

abstract：:We report here spectroscopic and biochemical data of a novel series of sugar-modified oligodeoxy-nucleotides, the carbocyclic oligothymidylates, c(dT)3-20. In c(dT)n a methylene group has been substituted for the oxygen atom of the deoxyribose ring of the natural thymidylate unit. c(dT)10-20 form helical structures, i...

journal_title：Nucleic acids research

authors： Sági J,Szemzõ A,Szécsi J,Otvös L

更新日期：1990-04-25 00:00:00

abstract：:During DNA double-strand break and replication fork repair by homologous recombination, the RAD51 recombinase catalyzes the DNA strand exchange reaction via a helical polymer assembled on single-stranded DNA, termed the presynaptic filament. Our published work has demonstrated a dual function of the SWI5-SFR1 complex ...

journal_title：Nucleic acids research

authors： Su GC,Yeh HY,Lin SW,Chung CI,Huang YS,Liu YC,Lyu PC,Chi P

更新日期：2016-07-27 00:00:00

abstract：:We describe snap-to-it probes, a novel probe technology to enhance the hybridization specificity of natural and unnatural nucleic acid oligomers using a simple and readily introduced structural motif. Snap-to-it probes were prepared from peptide nucleic acid (PNA) oligomers by modifying each terminus with a coordinati...

journal_title：Nucleic acids research

authors： Morgan JR,Lyon RP,Maeda DY,Zebala JA

更新日期：2008-06-01 00:00:00

abstract：:Site-directed RNA editing (SDRE) is a strategy to precisely alter genetic information within mRNAs. By linking the catalytic domain of the RNA editing enzyme ADAR to an antisense guide RNA, specific adenosines can be converted to inosines, biological mimics for guanosine. Previously, we showed that a genetically encod...

journal_title：Nucleic acids research

authors： Montiel-González MF,Vallecillo-Viejo IC,Rosenthal JJ

更新日期：2016-12-01 00:00:00

abstract：:MicroRNAs (miRNA) are short single-stranded RNA molecules derived from hairpin-forming precursors that play a crucial role as post-transcriptional regulators in eukaryotes and viruses. In the past years, many microRNA target genes (MTGs) have been identified experimentally. However, because of the high costs of experi...

journal_title：Nucleic acids research

authors： Leclercq M,Diallo AB,Blanchette M

更新日期：2017-01-25 00:00:00

abstract：:Ribosome biogenesis is fundamental for cellular life, but surprisingly little is known about the underlying pathway. In eukaryotes a comprehensive collection of experimentally verified ribosome biogenesis factors (RBFs) exists only for Saccharomyces cerevisiae. Far less is known for other fungi, animals or plants, and...

journal_title：Nucleic acids research

authors： Ebersberger I,Simm S,Leisegang MS,Schmitzberger P,Mirus O,von Haeseler A,Bohnsack MT,Schleiff E

更新日期：2014-02-01 00:00:00

abstract：:The Escherichia coli fmu gene product has recently been determined to be the 16S rRNA m(5)C 967 methyltransferase. As such, Fmu represents the first protein identified as an S -adenosyl-L-methionine (AdoMet)- dependent RNA m(5)C methyltransferase whose amino acid sequence is known. Using the amino acid sequence of Fmu...

journal_title：Nucleic acids research

authors： Reid R,Greene PJ,Santi DV

更新日期：1999-08-01 00:00:00

abstract：:RsaE is a regulatory RNA highly conserved amongst Firmicutes that lowers the amount of mRNAs associated with the TCA cycle and folate metabolism. A search for new RsaE targets in Staphylococcus aureus revealed that in addition to previously described substrates, RsaE down-regulates several genes associated with argini...

journal_title：Nucleic acids research

authors： Rochat T,Bohn C,Morvan C,Le Lam TN,Razvi F,Pain A,Toffano-Nioche C,Ponien P,Jacq A,Jacquet E,Fey PD,Gautheret D,Bouloc P

更新日期：2018-09-28 00:00:00

abstract：:RNA secondary structures, e.g. stem-loops that are often found at the 5' and 3' ends of mRNAs, are in many cases known to be crucial for transcript stability but their role in prolonging the lifetime of transcripts remains elusive. In this study we show for an essential RNA-stabilizing stem-loop at the 5' end of rbcL ...

journal_title：Nucleic acids research

authors： Suay L,Salvador ML,Abesha E,Klein U

更新日期：2005-08-22 00:00:00

abstract：:The solid phase synthesis of oligoribonucleotides using the H-phosphonate approach and the 1-[(2-chloro-4-methyl)phenyl]-4-methoxypiperidin-4-yl (Ctmp) and dimethoxytrityl (DMTr) groups, respectively, for the protection of the 2'- and 5'-hydroxy functions is described. The use of a new reagent, tris-(1,1,1,3,3,3-hexaf...

journal_title：Nucleic acids research

authors： Sakatsume O,Ohtsuki M,Takaku H,Reese CB

更新日期：1989-05-25 00:00:00

abstract：:Increasing evidence indicates that active DNA demethylation is involved in several processes in mammals, resulting in developmental stage-specificity and cell lineage-specificity. The recently discovered Ten-Eleven Translocation (TET) dioxygenases are accepted to be involved in DNA demethylation by initiating 5-mC oxi...

journal_title：Nucleic acids research

authors： Chen H,Kazemier HG,de Groote ML,Ruiters MH,Xu GL,Rots MG

更新日期：2014-02-01 00:00:00

abstract：:DNA breaks occur during many processes in mammalian cells, including recombination, repair, mutagenesis and apoptosis. Here we report a simple and rapid method for assaying DNA breaks and identifying DNA breaksites. Breaksites are first tagged and amplified by ligation-mediated PCR (LM-PCR), using nested PCR primers t...

journal_title：Nucleic acids research

authors： Kong Q,Maizels N

更新日期：2001-03-15 00:00:00

abstract：:A cross index is presented for using the improved selectivity offered by the Hung and Wensink (Nucl. Acids Res. 12, 1863-1874, 1984) method of partially filling in 5'-extensions produced by type II restriction endonucleases. After this treatment, DNA fragments which normally cannot be ligated to one another, can be jo...

journal_title：Nucleic acids research

authors： Korch C

更新日期：1987-04-24 00:00:00

abstract：:The European Nucleotide Archive (ENA; https://www.ebi.ac.uk/ena), provided from EMBL-EBI, has for more than three decades been responsible for archiving the world's public sequencing data and presenting this important resource to the scientific community to support and accelerate the global research effort. Here, we o...

journal_title：Nucleic acids research

authors： Harrison PW,Alako B,Amid C,Cerdeño-Tárraga A,Cleland I,Holt S,Hussein A,Jayathilaka S,Kay S,Keane T,Leinonen R,Liu X,Martínez-Villacorta J,Milano A,Pakseresht N,Rajan J,Reddy K,Richards E,Rosello M,Silvester N,Smi

更新日期：2019-01-08 00:00:00

abstract：:We present web servers for analysis of non-coding RNA sequences on the basis of their secondary structures. Software tools for structural multiple sequence alignments, structural pairwise sequence alignments and structural motif findings are available from the integrated web server and the individual stand-alone web s...

journal_title：Nucleic acids research

authors： Asai K,Kiryu H,Hamada M,Tabei Y,Sato K,Matsui H,Sakakibara Y,Terai G,Mituyama T

更新日期：2008-07-01 00:00:00

abstract：:We report the first investigation of oligoribonucleotides containing a few 1-(2-deoxy-2-alpha-C-hydroxymethyl-beta-D-ribofuranosyl)thymine units (or 2'-hm-dT, abbreviated in this work as 'H'). Both the 2'-CH2O-phosphoramidite and 3'-O-phosphoramidite derivatives of H were synthesized and incorporated into both 2',5'-R...

journal_title：Nucleic acids research

authors： Peng CG,Damha MJ

更新日期：2005-12-23 00:00:00

abstract：:Embryonic stem cells (ESCs) and meiosis are featured by relatively higher frequent homologous recombination associated with DNA double strand breaks (DSB) repair. Here, we show that Pold3 plays important roles in DSB repair, telomere maintenance and genomic stability of both ESCs and spermatocytes in mice. By attempti...

journal_title：Nucleic acids research

authors： Zhou Z,Wang L,Ge F,Gong P,Wang H,Wang F,Chen L,Liu L

更新日期：2018-04-20 00:00:00

abstract：:The design of a family shuffling strategy (CLERY: Combinatorial Libraries Enhanced by Recombination in Yeast) associating PCR-based and in vivo recombination and expression in yeast is described. This strategy was tested using human cytochrome P450 CYP1A1 and CYP1A2 as templates, which share 74% nucleotide sequence id...

journal_title：Nucleic acids research

authors： Abécassis V,Pompon D,Truan G

更新日期：2000-10-15 00:00:00

abstract：:Here we examined the role of cellular vitamin C in genotoxicity of carcinogenic chromium(VI) that requires reduction to induce DNA damage. In the presence of ascorbate (Asc), low 0.2-2 microM doses of Cr(VI) caused 10-15 times more chromosomal breakage in primary human bronchial epithelial cells or lung fibroblasts. D...

journal_title：Nucleic acids research

authors： Reynolds M,Stoddard L,Bespalov I,Zhitkovich A

更新日期：2007-01-01 00:00:00

abstract：:The recent publication of the Caenorhabditis elegans cisRED database has provided an extensive catalog of upstream elements that are conserved between nematode genomes. We have performed a secondary analysis to determine which subsequences of the cisRED motifs are found in multiple locations throughout the C. elegans ...

journal_title：Nucleic acids research

authors： Sleumer MC,Mah AK,Baillie DL,Jones SJ

更新日期：2010-05-01 00:00:00

abstract：:An attempt has been made to correlate differential scanning calorimetry melting profiles of 5S rRNAs from lupin seeds (L.s.) and wheat germ (W.g.) with their structure. It is suggested that the observed differences in thermal unfolding are due to differences in RNA nucleotide sequence and as a consequence in higher or...

journal_title：Nucleic acids research

authors： Barciszewski J,Bratek-Wiewiórowska MD,Górnicki P,Naskret-Barciszewska M,Wiewiórowski M,Zielenkiewicz A,Zielenkiewicz W

更新日期：1988-01-25 00:00:00

abstract：:MicroRNAs (miRNAs) are small noncoding RNAs that act as master regulators in many biological processes. miRNAs function mainly by downregulating the expression of their gene targets. Thus, accurate prediction of miRNA targets is critical for characterization of miRNA functions. To this end, we have developed an online...

journal_title：Nucleic acids research

authors： Chen Y,Wang X

更新日期：2020-01-08 00:00:00

abstract：:Here, we report a webserver for the improved SDM, used for predicting the effects of mutations on protein stability. As a pioneering knowledge-based approach, SDM has been highlighted as the most appropriate method to use in combination with many other approaches. We have updated the environment-specific amino-acid su...

journal_title：Nucleic acids research

authors： Pandurangan AP,Ochoa-Montaño B,Ascher DB,Blundell TL

更新日期：2017-07-03 00:00:00

abstract：:The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using (32)P-labeled material from T4-infected cultures of Escherichiacoli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAApsiCUGAUGAUGUGAGTpsiCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the class...

journal_title：Nucleic acids research

authors： Stahl S,Paddock GV,Abelson J

更新日期：1974-10-01 00:00:00

abstract：:Transcription of ribosomal RNA genes is generally accepted to correlate with cell growth. Using primary cultures of adult bovine aortic endothelial (ABAE) cells, we have shown that transcription of rDNA in confluent cells falls to 5% of the transcription level in growing cells. Protein kinase NII appears to be a limit...

journal_title：Nucleic acids research

authors： Belenguer P,Baldin V,Mathieu C,Prats H,Bensaid M,Bouche G,Amalric F

更新日期：1989-08-25 00:00:00

abstract：:Aberrant DNA methylation at CpG dinucleotides can result in epigenetic silencing of tumour suppressor genes and represents one of the earliest events in tumourigenesis. To date, however, high-throughput tools that are capable of surveying the methylation status of multiple gene promoters have been restricted to a limi...

journal_title：Nucleic acids research

authors： Mund C,Beier V,Bewerunge P,Dahms M,Lyko F,Hoheisel JD

更新日期：2005-04-28 00:00:00

abstract：:The B-subunits associated with the replicative DNA polymerases are conserved from Archaea to humans, whereas the corresponding catalytic subunits are not related. The latter belong to the B and D DNA polymerase families in eukaryotes and archaea, respectively. Sequence analysis places the B-subunits within the calcine...

journal_title：Nucleic acids research

authors： Jokela M,Eskelinen A,Pospiech H,Rouvinen J,Syväoja JE

更新日期：2004-04-30 00:00:00