Molecular basis of artifacts in the detection of telomerase activity and a modified primer for a more robust 'TRAP' assay.

abstract：:Differential expression analysis of RNA sequencing (RNA-seq) data typically relies on reconstructing transcripts or counting reads that overlap known gene structures. We previously introduced an intermediate statistical approach called differentially expressed region (DER) finder that seeks to identify contiguous regi...

journal_title：Nucleic acids research

authors： Collado-Torres L,Nellore A,Frazee AC,Wilks C,Love MI,Langmead B,Irizarry RA,Leek JT,Jaffe AE

更新日期：2017-01-25 00:00:00

abstract：:Therapeutic small interfering RNAs (siRNAs) are composed of chemically modified nucleotides, which enhance RNA stability and increase affinity in Watson-Crick base pairing. However, the precise fate of such modified nucleotides once the siRNA is degraded within the cell is unknown. Previously, we demonstrated that deo...

journal_title：Nucleic acids research

authors： Wu SY,Chen TM,Gmeiner WH,Chu E,Schmitz JC

更新日期：2013-04-01 00:00:00

abstract：:Long non-coding RNAs (lncRNAs) that have no protein-coding capacity make up a large proportion of the transcriptome of various species. Many lncRNAs are expressed within the animal central nervous system in spatial- and temporal-specific patterns, indicating that lncRNAs play important roles in cellular processes, neu...

journal_title：Nucleic acids research

authors： Li M,Wen S,Guo X,Bai B,Gong Z,Liu X,Wang Y,Zhou Y,Chen X,Liu L,Chen R

更新日期：2012-12-01 00:00:00

abstract：:The Rice Proteome Database is the first detailed database to describe the proteome of rice. The current release contains 21 reference maps based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) of proteins from rice tissues and subcellular compartments. These reference maps comprise 11 941 identified pr...

journal_title：Nucleic acids research

authors： Komatsu S,Kojima K,Suzuki K,Ozaki K,Higo K

更新日期：2004-01-01 00:00:00

abstract：:We have developed an effective post-PCR sterilization process and have applied the procedure to a diagnostic assay for HIV-1. The method, which is based on isopsoralen photochemistry, satisfies both the inactivation and hybridization requirements of a practical sterilization process. The key feature of the technique i...

journal_title：Nucleic acids research

authors： Isaacs ST,Tessman JW,Metchette KC,Hearst JE,Cimino GD

更新日期：1991-01-11 00:00:00

abstract：:The gene co-expression analysis toolbox (GeneCAT) introduces several novel microarray data analyzing tools. First, the multigene co-expression analysis, combined with co-expressed gene networks, provides a more powerful data mining technique than standard, single-gene co-expression analysis. Second, the high-throughpu...

journal_title：Nucleic acids research

authors： Mutwil M,Obro J,Willats WG,Persson S

更新日期：2008-07-01 00:00:00

abstract：:Two dimensional (2D) NMR and molecular dynamics simulations have been used to determine the three dimensional (3D) structure of a hairpin DNA, d-CTA-GAGGATCC-TUTT-GGATCCT (22mer; abbreviated as U2-hairpin), which has uracil at the second position from the 5' end of the tetraloop. The(1)H resonances of this hairpin hav...

journal_title：Nucleic acids research

authors： Ghosh M,Vinay Kumar N,Varshney U,Chary KV

更新日期：2000-05-01 00:00:00

abstract：:The small subunit (SSU) processome is a large ribonucleoprotein that is required for maturation of the 18S rRNA of the ribosome. Recently, a missense mutation in the C-terminus of an SSU processome protein, Utp4/Cirhin, was reported to cause North American Indian childhood cirrhosis (NAIC). In this study, we use Sacch...

journal_title：Nucleic acids research

authors： Freed EF,Baserga SJ

更新日期：2010-08-01 00:00:00

abstract：:The archaeal exosome is a phosphorolytic 3'-5' exoribonuclease complex. In a reverse reaction it synthesizes A-rich RNA tails. Its RNA-binding cap comprises the eukaryotic orthologs Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. In Sulfolobus solfataricus DnaG and Rrp4 but not Csl4 show preference f...

journal_title：Nucleic acids research

authors： Hou L,Klug G,Evguenieva-Hackenberg E

更新日期：2014-11-10 00:00:00

abstract：:Diadenosine 5',5'"-P1,P4-tetraphosphate (Ap4A) has been detected in cysts and developing embryos of the brine shrimp Artemia in amounts 10(4)-10(6) times lower than that of the guanine analogue, Gp4G. The unexpectedly high level of Ap4A in dormant cysts of 2.37 pmol/10(6) cells can be reduced to 0.03 pmol/10(6) cells ...

journal_title：Nucleic acids research

authors： McLennan AG,Prescott M

更新日期：1984-02-10 00:00:00

abstract：:Addition of thiol compounds containing an anionic group to the 3'-terminal unsaturated sugar of the 5' fragment obtained from an oligonucleotide containing an AP site cleaved by beta-elimination, can be followed by gel electrophoresis. The technique enables to distinguish between two mechanisms of cleavage of the C3'-...

journal_title：Nucleic acids research

authors： Bailly V,Verly WG

更新日期：1988-10-25 00:00:00

abstract：:The sequence of 436 nucleotides around the region coding for 5.8S RNA in the Sciara coprophila rDNA transcription unit (1) has been determined. Regions coding for 5.8S and 2S RNAs have been identified; they are 80 - 90% homologous to the corresponding Drosophila sequences and are separated by a 22 nucleotide long spac...

journal_title：Nucleic acids research

authors： Jordan BR,Latil-Damotte M,Jourdan R

更新日期：1980-08-25 00:00:00

abstract：:Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor expressed in metazoan cells that is responsible for eliciting the production of type I interferons and pro-inflammatory cytokines upon detection of intracellular, non-self RNA. Structural studies of RIG-I have identified a novel Pincer domain com...

journal_title：Nucleic acids research

authors： Rawling DC,Kohlway AS,Luo D,Ding SC,Pyle AM

更新日期：2014-10-01 00:00:00

abstract：:Tunable induction of gene expression is an essential tool in biology and biotechnology. In spite of that, current induction systems often exhibit unpredictable behavior and performance shortcomings, including high sensitivity to transactivator dosage and plasmid take-up variation, and excessive consumption of cellular...

journal_title：Nucleic acids research

authors： Lillacci G,Benenson Y,Khammash M

更新日期：2018-10-12 00:00:00

abstract：:SUPERFAMILY provides structural, functional and evolutionary information for proteins from all completely sequenced genomes, and large sequence collections such as UniProt. Protein domain assignments for over 900 genomes are included in the database, which can be accessed at http://supfam.org/. Hidden Markov models ba...

journal_title：Nucleic acids research

authors： Wilson D,Pethica R,Zhou Y,Talbot C,Vogel C,Madera M,Chothia C,Gough J

更新日期：2009-01-01 00:00:00

abstract：:Equilibrium and kinetics of thermal melting of yeast 5S ribosomal RNA in aqueous NaCl with or without Mg2+ were investigated by differential thermal melting and temperature jump methods. Two peaks (1 and 2) and a shoulder were observed in each of melting curves at ionic strength I=0.002-0.5 and linearity between each ...

journal_title：Nucleic acids research

authors： Ohta S,Maruyama S,Nitta K,Sugai S

更新日期：1983-05-25 00:00:00

abstract：:The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs. ...

journal_title：Nucleic acids research

authors： Smith CL,Matsumoto T,Niwa O,Klco S,Fan JB,Yanagida M,Cantor CR

更新日期：1987-06-11 00:00:00

abstract：:Present results provide direct evidence of the nature of a conformational change in DNA when nucleosomes are formed from core histones and poly [d(A-T)]. First, we have found some features which have characteristic aspects of the A like conformation of DNA. Thus, an increased contribution due to a sugar conformation c...

journal_title：Nucleic acids research

authors： Brahms S,Brahmachari SK,Angelier N,Brahms JG

更新日期：1981-10-10 00:00:00

abstract：:The 5'-cap of spliceosomal small nuclear RNAs, some small nucleolar RNAs and of telomerase RNA was found to be hypermethylated in vivo. The Trimethylguanosine Synthase 1 (TGS1) mediates this conversion of the 7-methylguanosine-cap to the 2,2,7-trimethylguanosine (m(3)G)-cap during maturation of the RNPs. For mammalian...

journal_title：Nucleic acids research

authors： Monecke T,Dickmanns A,Ficner R

更新日期：2009-07-01 00:00:00

abstract：:The Plant Ontology Consortium (POC, http://www.plantontology.org) is a collaborative effort among model plant genome databases and plant researchers that aims to create, maintain and facilitate the use of a controlled vocabulary (ontology) for plants. The ontology allows users to ascribe attributes of plant structure ...

journal_title：Nucleic acids research

authors： Avraham S,Tung CW,Ilic K,Jaiswal P,Kellogg EA,McCouch S,Pujar A,Reiser L,Rhee SY,Sachs MM,Schaeffer M,Stein L,Stevens P,Vincent L,Zapata F,Ware D

更新日期：2008-01-01 00:00:00

abstract：:A C-->U mutation (rdn5) in the conserved sarcin/ricin domain of yeast 25S rRNA has been shown to cause translational suppression and paromomycin resistance. It also separates the killing from the misreading effect of this antibiotic. We confirm these findings and provide in vitro evidence that rdn5 causes a 3-fold inc...

journal_title：Nucleic acids research

authors： Panopoulos P,Dresios J,Synetos D

更新日期：2004-10-11 00:00:00

abstract：:Inducible promoters are a central regulatory component in synthetic biology, metabolic engineering, and protein production for laboratory and commercial uses. Many of these applications utilize two or more exogenous promoters, imposing a currently unquantifiable metabolic burden on the living system. Here, we engineer...

journal_title：Nucleic acids research

authors： Davey JA,Wilson CJ

更新日期：2020-09-25 00:00:00

abstract：:Biological networks are a topic of great current interest, particularly with the publication of a number of large genome-wide interaction datasets. They are globally characterized by a variety of graph-theoretic statistics, such as the degree distribution, clustering coefficient, characteristic path length and diamete...

journal_title：Nucleic acids research

authors： Yu H,Zhu X,Greenbaum D,Karro J,Gerstein M

更新日期：2004-01-14 00:00:00

abstract：:Protein-DNA binding is a fundamental component of gene regulatory processes, but it is still not completely understood how proteins recognize their target sites in the genome. Besides hydrogen bonding in the major groove (base readout), proteins recognize minor-groove geometry using positively charged amino acids (sha...

journal_title：Nucleic acids research

authors： Chiu TP,Rao S,Mann RS,Honig B,Rohs R

更新日期：2017-12-01 00:00:00

abstract：:The sequences coding for DNA[cytosine-N4]methyltransferases MvaI (from Micrococcus varians RFL19) and Cfr9I (from Citrobacter freundii RFL9) have been determined. The predicted methylases are proteins of 454 and 300 amino acids, respectively. Primary structure comparison of M.Cfr9I and another m4C-forming methylase, M...

journal_title：Nucleic acids research

authors： Klimasauskas S,Timinskas A,Menkevicius S,Butkienè D,Butkus V,Janulaitis A

更新日期：1989-12-11 00:00:00

abstract：:Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to ...

journal_title：Nucleic acids research

authors： Al-Hakim AH,Hull R

更新日期：1986-12-22 00:00:00

abstract：:Trinucleotide repeat (TNR) expansions cause at least 17 heritable neurological diseases, including Huntington's disease. Expansions are thought to arise from abnormal processing of TNR DNA by specific trans-acting proteins. For example, the DNA repair complex MutSβ (MSH2-MSH3 heterodimer) is required in mice for on-go...

journal_title：Nucleic acids research

authors： Gannon AM,Frizzell A,Healy E,Lahue RS

更新日期：2012-11-01 00:00:00

abstract：:Protein p56 (56 amino acids) from the Bacillus subtilis phage 29 inactivates the host uracil-DNA glycosylase (UDG), an enzyme involved in the base excision repair pathway. At present, p56 is the only known example of a UDG inhibitor encoded by a non-uracil containing viral DNA. Using analytical ultracentrifugation met...

journal_title：Nucleic acids research

authors： Serrano-Heras G,Ruiz-Masó JA,del Solar G,Espinosa M,Bravo A,Salas M

更新日期：2007-01-01 00:00:00

abstract：:Every bulky lesion in DNA can potentially inhibit the Taq DNA polymerase and thereby decrease the amplification produced in the polymerase chain reaction. We investigated the feasibility of using this inhibition to quantify DNA lesions produced by the anticancer drug cisplatin. Products were detected by electrophoresi...

journal_title：Nucleic acids research

authors： Jennerwein MM,Eastman A

更新日期：1991-11-25 00:00:00

abstract：:Proteins that catalyse homologous recombination have been identified in all living organisms and are essential for the repair of damaged DNA as well as for the generation of genetic diversity. In bacteria homologous recombination is performed by the RecA protein, whereas in the eukarya a related protein called Rad51 i...

journal_title：Nucleic acids research

authors： McIlwraith MJ,Hall DR,Stasiak AZ,Stasiak A,Wigley DB,West SC

更新日期：2001-11-15 00:00:00