Studies towards the development of chemically synthesized non-radioactive biotinylated nucleic acid hybridization probes.


:Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to nucleic acids (DNA) via the bifunctional cross-linking reagents, glutaraldehyde, 1,2,7,8-diepoxyoctane, bis (succinimidyl) suberate or bis (sulfonosuccinimidyl) suberate. This provides a very sensitive probe by which as little as between 10-50fg of target DNA can be visualized using dot-blot hybridization procedures in conjunction with avidin or streptavidin enzyme conjugates.


Nucleic Acids Res


Nucleic acids research


Al-Hakim AH,Hull R




Has Abstract


1986-12-22 00:00:00












  • Structural determinants of an internal ribosome entry site that direct translational reading frame selection.

    abstract::The dicistrovirus intergenic internal ribosome entry site (IGR IRES) directly recruits the ribosome and initiates translation using a non-AUG codon. A subset of IGR IRESs initiates translation in either of two overlapping open reading frames (ORFs), resulting in expression of the 0 frame viral structural polyprotein a...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ren Q,Au HH,Wang QS,Lee S,Jan E

    更新日期:2014-08-01 00:00:00

  • Effects of diaminopurine and inosine substitutions on A-tract induced DNA curvature. Importance of the 3'-A-tract junction.

    abstract::Gel migration and uranyl photoprobing have been used to study the effects of inosine and 2,6-diaminopurine (2,6-DAP) substitution on adenine-tract (A-tract) induced DNA curvature. Using a 10mer repeated sequence including five inosines we show by uranyl photoprobing that a narrow minor groove varying in phase with the...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Mollegaard NE,Bailly C,Waring MJ,Nielsen PE

    更新日期:1997-09-01 00:00:00

  • Two closely spaced promoters are equally activated by a remote enhancer: evidence against a scanning model for enhancer action.

    abstract::To explain the activation of transcription by a remote enhancer, two models are most often considered, namely looping and scanning. A scanning model, also referred to as 'polymerase entry site' model predicts that for two adjacent promoters the one proximal to an enhancer would be preferentially activated. Preferentia...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Heuchel R,Matthias P,Schaffner W

    更新日期:1989-11-25 00:00:00

  • Identification of proteins interacting with newly replicated DNA in SV40-infected cells by UV-induced DNA-protein cross-linking.

    abstract::Protein species interacting with newly replicated DNA were analyzed using a photo cross-linking technique. Nascent DNA was labeled in vitro with [alpha-32P]dCTP and BrdUTP in SV40-infected CV-1 cells made permeable with saponin. The labeled cells were then irradiated with UV light (254 nm) and were treated extensively...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tsutsui K,Watanabe S,Katagiri M,Oda T

    更新日期:1983-07-25 00:00:00

  • Spatial localization of co-regulated genes exceeds genomic gene clustering in the Saccharomyces cerevisiae genome.

    abstract::While it has been long recognized that genes are not randomly positioned along the genome, the degree to which its 3D structure influences the arrangement of genes has remained elusive. In particular, several lines of evidence suggest that actively transcribed genes are spatially co-localized, forming transcription fa...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ben-Elazar S,Yakhini Z,Yanai I

    更新日期:2013-02-01 00:00:00

  • 3' Alu PCR: a simple and rapid method to isolate human polymorphic markers.

    abstract::Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Charlieu JP,Laurent AM,Carter DA,Bellis M,Roizès G

    更新日期:1992-03-25 00:00:00

  • Roles of yeast eIF2α and eIF2β subunits in the binding of the initiator methionyl-tRNA.

    abstract::Heterotrimeric eukaryotic/archaeal translation initiation factor 2 (e/aIF2) binds initiator methionyl-tRNA and plays a key role in the selection of the start codon on messenger RNA. tRNA binding was extensively studied in the archaeal system. The γ subunit is able to bind tRNA, but the α subunit is required to reach h...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Naveau M,Lazennec-Schurdevin C,Panvert M,Dubiez E,Mechulam Y,Schmitt E

    更新日期:2013-01-01 00:00:00

  • Ionic strength effects on the stability and conformation of Penicillium chrysogenum mycophage double stranded RNA.

    abstract::The effect of [Na+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stabili...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Burnett JP,Frank BH,Douthart RJ

    更新日期:1975-06-01 00:00:00

  • A new member of the plasma protease inhibitor gene family.

    abstract::A 2.1-kb cDNA clone representing a new member of the protease inhibitor family was isolated from a human liver cDNA library. The inhibitor, named human Leuserpin 2 (hLS2), comprises 480 amino acids and contains a leucine residue at its putative reactive center. HLS2 is about 25-28% homologous to three human members of...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Ragg H

    更新日期:1986-01-24 00:00:00

  • The secondary structure of E. coli ribosomes and ribosomal RNA's: a spectrophotometric approach.

    abstract::The thermal denaturation spectra of E. coli 50s and 30s ribosomal sub units and of their isolated RNA's were studied over the wavelength range of 2300-3000 Angstrom. It was possible to fit the experimental denaturation spectra with super positions of the reference spectra for denaturation of A - U and G - C base pairs...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Araco A,Belli M,Giorgi C,Onori G

    更新日期:1975-03-01 00:00:00

  • PARP1 catalytic variants reveal branching and chain length-specific functions of poly(ADP-ribose) in cellular physiology and stress response.

    abstract::Poly(ADP-ribosyl)ation regulates numerous cellular processes like genome maintenance and cell death, thus providing protective functions but also contributing to several pathological conditions. Poly(ADP-ribose) (PAR) molecules exhibit a remarkable heterogeneity in chain lengths and branching frequencies, but the biol...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Aberle L,Krüger A,Reber JM,Lippmann M,Hufnagel M,Schmalz M,Trussina IREA,Schlesiger S,Zubel T,Schütz K,Marx A,Hartwig A,Ferrando-May E,Bürkle A,Mangerich A

    更新日期:2020-10-09 00:00:00

  • Identification of DNA--cisplatin adducts in a blind trial of in situ scanning tunneling microscopy.

    abstract::Scanning tunneling microscopy (STM) reveals nanometer scale details of hydrated DNA but the interpretation of the images is controversial because of substrate artifacts and the lack of a theory for image contrast. We demonstrate that we have overcome these problems by identifying five DNA samples by their STM images a...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Jeffrey AM,Jing TW,DeRose JA,Vaught A,Rekesh D,Lu FX,Lindsay SM

    更新日期:1993-12-25 00:00:00

  • Characterization of human 5S rRNA genes.

    abstract::The human 5S rRNA genes are found in clusters of tandem repeated units. We have cloned and partially characterized six restriction fragments from two clusters of 2.3 kb and 1.6 kb repeats, respectively. Four fragments from the cluster of 2.3 kb repeats contain a 5S rRNA gene and one fragment contains a gene variant wi...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sørensen PD,Frederiksen S

    更新日期:1991-08-11 00:00:00

  • Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.

    abstract::We previously showed that mRNAs synthesized from three genes that naturally lack introns contain a portion of their coding sequence, known as a cytoplasmic accumulation region (CAR), which is essential for stable accumulation of the intronless mRNAs in the cytoplasm. The CAR in each mRNA is unexpectedly large, ranging...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lei H,Zhai B,Yin S,Gygi S,Reed R

    更新日期:2013-02-01 00:00:00

  • High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands.

    abstract::We have isolated 2'-Fluoro-substituted RNA aptamers that bind to streptavidin (SA) with an affinity around 7 +/- 1.8 nM, comparable with that of recently described peptide aptamers. Binding to SA was not prevented by prior saturation with biotin, enabling nucleic acid aptamers to form useful ternary complexes. Mutagen...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Tahiri-Alaoui A,Frigotto L,Manville N,Ibrahim J,Romby P,James W

    更新日期:2002-05-15 00:00:00

  • Coding capacity of complementary DNA strands.

    abstract::A Fortran computer algorithm has been used to analyze the nucleotide sequence of several structural genes. The analysis performed on both coding and complementary DNA strands shows that whereas open reading frames shorter than 100 codons are randomly distributed on both DNA strands, open reading frames longer than 100...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Casino A,Cipollaro M,Guerrini AM,Mastrocinque G,Spena A,Scarlato V

    更新日期:1981-03-25 00:00:00

  • Crystal structure of the P2 C-repressor: a binder of non-palindromic direct DNA repeats.

    abstract::As opposed to the vast majority of prokaryotic repressors, the immunity repressor of temperate Escherichia coli phage P2 (C) recognizes non-palindromic direct repeats of DNA rather than inverted repeats. We have determined the crystal structure of P2 C at 1.8 Å. This constitutes the first structure solved from the fam...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Massad T,Skaar K,Nilsson H,Damberg P,Henriksson-Peltola P,Haggård-Ljungquist E,Högbom M,Stenmark P

    更新日期:2010-11-01 00:00:00

  • Dimerization specificity of all 67 B-ZIP motifs in Arabidopsis thaliana: a comparison to Homo sapiens B-ZIP motifs.

    abstract::Basic region-leucine zipper (B-ZIP) proteins are a class of dimeric sequence-specific DNA-binding proteins unique to eukaryotes. We have identified 67 B-ZIP proteins in the Arabidopsis thaliana genome. No A.thaliana B-ZIP domains are homologous with any Homo sapiens B-ZIP domains. Here, we predict the dimerization spe...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Deppmann CD,Acharya A,Rishi V,Wobbes B,Smeekens S,Taparowsky EJ,Vinson C

    更新日期:2004-06-29 00:00:00

  • Spatial configuration of deoxyribotrinucleoside diphosphates in aqueous solution.

    abstract::The detailed conformational features and dynamics of the naturally occurring deoxyribotrinucleoside diphosphates d-TpTpT and d-TpTpC have been investigated at 20 degrees C and 80 degrees C in aqueous solution by nuclear magnetic resonance spectroscopy. The observed NMR parameters indicate that the conformational prope...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Cheng DM,Dhingra MM,Sarma RH

    更新日期:1978-11-01 00:00:00

  • DRB4 dsRBD1 drives dsRNA recognition in Arabidopsis thaliana tasi/siRNA pathway.

    abstract::In Arabidopsis thaliana, endogenous trans-acting and exogenous siRNA pathways are initiated by the interaction of DRB4 with trigger dsRNA. Further, DCL4:DRB4 complex cleaves the dsRNA into 21 bp siRNA. Understanding molecular determinants and mechanistic details of dsRNA recognition by DRB4 is vital for inducing long-...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Chiliveri SC,Aute R,Rai U,Deshmukh MV

    更新日期:2017-08-21 00:00:00

  • RAD51 and mitotic function of mus81 are essential for recovery from low-dose of camptothecin in the absence of the WRN exonuclease.

    abstract::Stabilization of stalled replication forks prevents excessive fork reversal or degradation, which can undermine genome integrity. The WRN protein is unique among the other human RecQ family members to possess exonuclease activity. However, the biological role of the WRN exonuclease is poorly defined. Recently, the WRN...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Aiello FA,Palma A,Malacaria E,Zheng L,Campbell JL,Shen B,Franchitto A,Pichierri P

    更新日期:2019-07-26 00:00:00

  • The Zebrafish Information Network: the zebrafish model organism database provides expanded support for genotypes and phenotypes.

    abstract::The Zebrafish Information Network (ZFIN,, the model organism database for zebrafish, provides the central location for curated zebrafish genetic, genomic and developmental data. Extensive data integration of mutant phenotypes, genes, expression patterns, sequences, genetic markers, morpholinos, map po...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sprague J,Bayraktaroglu L,Bradford Y,Conlin T,Dunn N,Fashena D,Frazer K,Haendel M,Howe DG,Knight J,Mani P,Moxon SA,Pich C,Ramachandran S,Schaper K,Segerdell E,Shao X,Singer A,Song P,Sprunger B,Van Slyke CE,Weste

    更新日期:2008-01-01 00:00:00

  • A computer program to enter DNA gel reading data into a computer.

    abstract::This paper describes a simple program that uses a digitizing device to enter DNA sequences directly from autoradiographs into a computer. ...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Staden R

    更新日期:1984-01-11 00:00:00

  • Transcripts within the replication origin, oriC, of Escherichia coli.

    abstract::Transcription start and termination sites were mapped in the E. coli replication origin, oriC. Outward transcription from within oriC (promoters Pori-r and Pori-l) was found to start in vivo at position 178 for Pori-l and at positions 294 and 304 for Pori-r, respectively. These transcripts were terminated after 100-15...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Schauzu MA,Kücherer C,Kölling R,Messer W,Lother H

    更新日期:1987-03-25 00:00:00

  • Intracellular inhibition of hepatitis C virus (HCV) internal ribosomal entry site (IRES)-dependent translation by peptide nucleic acids (PNAs) and locked nucleic acids (LNAs).

    abstract::Hepatitis C virus (HCV) is the major etiological agent of non-A, non-B hepatitis. Current therapies are not effective in all patients and can result in the generation of resistant mutants, leading to a need for new therapeutic options. HCV has an RNA genome that contains a well-defined and highly conserved secondary s...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Nulf CJ,Corey D

    更新日期:2004-07-19 00:00:00

  • The regulatory region of the divergent argECBH operon in Escherichia coli K-12.

    abstract::The nucleotide sequence of the control region of the divergent argECBH operon has been established in the wild type and in mutants affecting expression of these genes. The argE and argCBH promoters face each other and overlap with an operator region containing two domains which may act as distinct repressor binding si...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Piette J,Cunin R,Boyen A,Charlier D,Crabeel M,Van Vliet F,Glansdorff N,Squires C,Squires CL

    更新日期:1982-12-20 00:00:00

  • Genome-wide prediction and analysis of human chromatin boundary elements.

    abstract::Boundary elements partition eukaryotic chromatin into active and repressive domains, and can also block regulatory interactions between domains. Boundary elements act via diverse mechanisms making accurate feature-based computational predictions difficult. Therefore, we developed an unbiased algorithm that predicts th...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Wang J,Lunyak VV,Jordan IK

    更新日期:2012-01-01 00:00:00

  • CUBAP: an interactive web portal for analyzing codon usage biases across populations.

    abstract::Synonymous codon usage significantly impacts translational and transcriptional efficiency, gene expression, the secondary structure of both mRNA and proteins, and has been implicated in various diseases. However, population-specific differences in codon usage biases remain largely unexplored. Here, we present a web se...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Hodgman MW,Miller JB,Meurs TE,Kauwe JSK

    更新日期:2020-11-04 00:00:00

  • PolyA_DB 2: mRNA polyadenylation sites in vertebrate genes.

    abstract::Polyadenylation of nascent transcripts is one of the key mRNA processing events in eukaryotic cells. A large number of human and mouse genes have alternative polyadenylation sites, or poly(A) sites, leading to mRNA variants with different protein products and/or 3'-untranslated regions (3'-UTRs). PolyA_DB 2 contains p...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Lee JY,Yeh I,Park JY,Tian B

    更新日期:2007-01-01 00:00:00

  • Sequence-dependent off-target inhibition of TLR7/8 sensing by synthetic microRNA inhibitors.

    abstract::Anti-microRNA (miRNA) oligonucleotides (AMOs) with 2'-O-Methyl (2'OMe) residues are commonly used to study miRNA function and can achieve high potency, with low cytotoxicity. Not withstanding this, we demonstrate the sequence-dependent capacity of 2'OMe AMOs to inhibit Toll-like receptor (TLR) 7 and 8 sensing of immun...

    journal_title:Nucleic acids research

    pub_type: 杂志文章


    authors: Sarvestani ST,Stunden HJ,Behlke MA,Forster SC,McCoy CE,Tate MD,Ferrand J,Lennox KA,Latz E,Williams BR,Gantier MP

    更新日期:2015-01-01 00:00:00