Studies towards the development of chemically synthesized non-radioactive biotinylated nucleic acid hybridization probes.

Abstract:

:Non-radioactive nucleic acid hybridization probes have been constructed in which the reporter group is long chain biotin chemically linked to a basic macromolecule (histone H1, cytochrome C or polyethyleneimine). The modified basic macromolecule which carries many biotin residues can, in turn, be covalently linked to nucleic acids (DNA) via the bifunctional cross-linking reagents, glutaraldehyde, 1,2,7,8-diepoxyoctane, bis (succinimidyl) suberate or bis (sulfonosuccinimidyl) suberate. This provides a very sensitive probe by which as little as between 10-50fg of target DNA can be visualized using dot-blot hybridization procedures in conjunction with avidin or streptavidin enzyme conjugates.

journal_name

Nucleic Acids Res

journal_title

Nucleic acids research

authors

Al-Hakim AH,Hull R

doi

10.1093/nar/14.24.9965

subject

Has Abstract

pub_date

1986-12-22 00:00:00

pages

9965-76

issue

24

eissn

0305-1048

issn

1362-4962

journal_volume

14

pub_type

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