Abstract:
:A novel approach for the amplification of cDNA ends is described. It requires only minimal amounts of material, a simple cDNA synthesis reaction and a single PCR reaction to amplify the desired 5'- or 3'-ends of a certain cDNA of interest. It combines the so called CapFinder approach with solid phase cDNA synthesis, thus almost eliminating background problems usually associated with 5'-RACE protocols. This approach could be used to generate complete 5'-ends of numerous cDNAs using only one cDNA synthesis reaction. In combination with LA PCR, several kilobases of unknown 5'-ends could be amplified. It is easy to perform, quick, inexpensive and reliable, which should enable it to replace most currently used 5'-RACE protocols.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Schramm G,Bruchhaus I,Roeder Tdoi
10.1093/nar/28.22.e96keywords:
subject
Has Abstractpub_date
2000-11-15 00:00:00pages
E96issue
22eissn
0305-1048issn
1362-4962journal_volume
28pub_type
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