Abstract:
:Over 50 introns have been reported in archaeal rRNA genes (rDNAs), a subset of which nests putative homing endonuclease (HEase) genes. Here, we report the identification and characterization of a novel archaeal LAGLIDADG-type HEase, I-ApeKI [corrected], encoded by the ApeK1.S908 intron within the 16S rDNA of Aeropyrum pernix K1. I-ApeKI [corrected] consists of 222 amino acids and harbors two LAGLIDADG-like sequences. It recognizes the 20 bp non-palindromic sequence 5'-GCAAGGCTGAAAC downward arrowTTAAAGG and cleaves target DNA to produce protruding tetranucleotide 3' ends. Either Mn2+ or Co2+ can be substituted for Mg2+ as a cofactor in the cleavage reaction. Of the 20 bases within the minimal recognition site, 7 are essential for cleavage and are located at positions proximal to the cleavage sites.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Nomura N,Morinaga Y,Shirai N,Sako Ydoi
10.1093/nar/gni118keywords:
subject
Has Abstractpub_date
2005-07-26 00:00:00pages
e116issue
13eissn
0305-1048issn
1362-4962pii
33/13/e116journal_volume
33pub_type
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