Abstract：
:BRENDA is a comprehensive relational database on functional and molecular information of enzymes, based on primary literature. The database contains information extracted and evaluated from approximately 46 000 references, holding data of at least 40 000 different enzymes from more than 6900 different organisms, classified in approximately 3900 EC numbers. BRENDA is an important tool for biochemical and medical research covering information on properties of all classified enzymes, including data on the occurrence, catalyzed reaction, kinetics, substrates/products, inhibitors, cofactors, activators, structure and stability. All data are connected to literature references which in turn are linked to PubMed. The data and information provide a fundamental tool for research of enzyme mechanisms, metabolic pathways, the evolution of metabolism and, furthermore, for medicinal diagnostics and pharmaceutical research. The database is a resource for data of enzymes, classified according to the EC system of the IUBMB Enzyme Nomenclature Committee, and the entries are cross-referenced to other databases, i.e. organism classification, protein sequence, protein structure and literature references. BRENDA provides an academic web access at http://www.brenda.uni-koeln.de.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Schomburg I,Chang A,Schomburg Ddoi
10.1093/nar/30.1.47keywords:
subject
Has Abstractpub_date
2002-01-01 00:00:00pages
47-9issue
1eissn
0305-1048issn
1362-4962journal_volume
30pub_type
杂志文章abstract：:Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia coli of several gene activities independently and quantitatively. The novel promoter PLtetO-1 allows the regulation of ge...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/25.6.1203
更新日期：1997-03-15 00:00:00
abstract：:IS200 is found throughout Enterobacteriaceae and transposes at a notoriously low frequency. In addition to the transposase protein (TnpA), IS200 encodes an uncharacterized Hfq-binding sRNA that is encoded opposite to the tnpA 5'UTR. In the current work we asked if this sRNA represses tnpA expression. We show here that...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv584
更新日期：2015-07-27 00:00:00
abstract：:FIMM database (http://sdmc.krdl.org.sg:8080/fimm) contains data relevant to functional molecular immunology, focusing on cellular immunology. It contains fully referenced data on protein antigens, major histocompatibility complex (MHC) molecules, MHC-associated peptides and relevant disease associations. FIMM has a se...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/30.1.226
更新日期：2002-01-01 00:00:00
abstract：:This report documents the error rate in a commercially distributed subset of the IMAGE Consortium mouse cDNA clone collection. After isolation of plasmid DNA from 1189 bacterial stock cultures, only 62. 2% were uncontaminated and contained cDNA inserts that had significant sequence identity to published data for the o...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.2.582
更新日期：2001-01-15 00:00:00
abstract：:The log kobs vs. pH profiles were determined in the intermediate acidity region for the glycosyl hydrolysis of guanosine and its 8-amino, 8-monomethylamino, 8-dimethylamino and 8-bromo derivatives. The decreased rate of the 8-amino and enhanced rate of the 8-bromo compound compared to guanosine support an A type mecha...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/4.3.697
更新日期：1977-03-01 00:00:00
abstract：:FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase). The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. FlyBase is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.1.53
更新日期：1996-01-01 00:00:00
abstract：:The ARF tumour suppressor stabilizes p53 by negatively regulating the E3 ubiquitin ligase MDM2 to promote cell cycle arrest and cell death. However, ARF is also able to arrest cell proliferation by inhibiting ribosome biogenesis. In greater part this is achieved by targeting the transcription termination factor I (TTF...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gks198
更新日期：2012-07-01 00:00:00
abstract：:The DNA unwinding element (DUE) is a sequence rich in adenine and thymine residues present within the origin region of both prokaryotic and eukaryotic replicons. Recently, it has been shown that this is the site where bacterial DnaA proteins, the chromosomal replication initiators, form a specific nucleoprotein filame...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku453
更新日期：2014-07-01 00:00:00
abstract：:Programmed ribosomal frameshifting provides a mechanism to decode information located in two overlapping reading frames by diverting a proportion of translating ribosomes into a second open reading frame (ORF). The result is the production of two proteins: the product of standard translation from ORF1 and an ORF1-ORF2...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkl531
更新日期：2006-01-01 00:00:00
abstract：:R.E.D. Server is a unique, open web service, designed to derive non-polarizable RESP and ESP charges and to build force field libraries for new molecules/molecular fragments. It provides to computational biologists the means to derive rigorously molecular electrostatic potential-based charges embedded in force field l...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr288
更新日期：2011-07-01 00:00:00
abstract：:Dictyostelium discoideum is of increasing interest as a model eukaryotic cell because its many attributes have recently been expanded to include improved genetic and biochemical manipulability. The ability to transform Dictyostelium using drug resistance as a selectable marker (1) and to gene target by high frequency ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/16.14.6617
更新日期：1988-07-25 00:00:00
abstract：:The genetic etiology of premature ovarian insufficiency (POI) has been well established to date, however, the role of long noncoding RNAs (lncRNAs) in POI is largely unknown. In this study, we identified a down-expressed lncRNA HCP5 in granulosa cells (GCs) from biochemical POI (bPOI) patients, which impaired DNA dama...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa127
更新日期：2020-05-07 00:00:00
abstract：:Microbial biosynthetic gene clusters are a valuable source of bioactive molecules. However, because they typically represent a small fraction of genomic material in most metagenomic samples, it remains challenging to deeply sequence them. We present an approach to isolate and sequence gene clusters in metagenomic samp...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa131
更新日期：2020-05-07 00:00:00
abstract：:Despite much information as to the structure and function of the general transcription factors, little is known about the regulation of their expression. Transcription of the Saccharomyces cerevisiae SUA7 (TFIIB) gene results in the formation of two discrete transcripts. It was originally reported that the two transcr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.22.4435
更新日期：2000-11-15 00:00:00
abstract：:Yeast tRNA Lys2 codes preferentially for AAA and contains a 2-thiouridine derivative (U) at the 5'-position of the anticodon. Removal of the 2-thio group from U by treatment with CNBr did not affect the amino acid accepting activity of the modified tRNA Lys2. CNBr treated tRNA Lys2 was active in protein synthesis but ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.3.523
更新日期：1976-03-01 00:00:00
abstract：:We previously proposed that the first enzyme for histidine biosynthesis in Salmonellatyphimurium plays a role in regulating expression of the histidine operon and that in order to play this role the enzyme must form a complex with histidyl-tRNA. Among the many observations that led to these conclusions were 1) that re...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/1.7.881
更新日期：1974-07-01 00:00:00
abstract：:The DNA repair enzyme human uracil DNA glycosylase (UNG) scans short stretches of genomic DNA and captures rare uracil bases as they transiently emerge from the DNA duplex via spontaneous base pair breathing motions. The process of DNA scanning requires that the enzyme transiently loosen its grip on DNA to allow stoch...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp161
更新日期：2009-06-01 00:00:00
abstract：:A cell-free extract of yeast nuclei that can specifically transcribe cloned yeast 5S rRNA genes has been developed. Optima for transcription of 5S rDNA were determined and conditions of extract preparation leading to reproducible activities and specificities established. The major in vitro product has the same size an...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/10.17.5223
更新日期：1982-09-11 00:00:00
abstract：:We present evidence of formation of an intramolecular parallel triple helix with T*A.T and G*G.C base triplets (where * represents the hydrogen bonding interaction between the third strand and the duplex while. represents the Watson-Crick interactions which stabilize the duplex). The third GT strand, containing seven ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/28.18.3511
更新日期：2000-09-15 00:00:00
abstract：:DNA double-strand breaks (DSBs) are repaired by either the non-homologous end joining (NHEJ) or homologous recombination (HR) pathway. Pathway choice is determined by the generation of 3΄ single-strand DNA overhangs at the break that are initiated by the action of the Mre11-Rad50-Xrs2 (MRX) complex to direct repair to...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx221
更新日期：2017-06-02 00:00:00
abstract：:Early in its differentiative pathway, a given B lymphocyte expresses immunoglobulin of the mu heavy chain class (IgM). Subsequent differentiative processes may involve rearrangement within the immunoglobulin heavy chain chromosomal locus to enable cells of the same lineage to synthesize immunoglobulins of other heavy ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/13.1.225
更新日期：1985-01-11 00:00:00
abstract：:RNA is emerging as a key regulator of a plethora of biological processes. While its study has remained elusive for decades, the recent advent of high-throughput sequencing technologies provided the unique opportunity to develop novel techniques for the study of RNA structure and post-transcriptional modifications. Non...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky486
更新日期：2018-09-19 00:00:00
abstract：:Signaling pathways constitute a valuable source of information that allows interpreting the way in which alterations in gene activities affect to particular cell functionalities. There are web tools available that allow viewing and editing pathways, as well as representing experimental data on them. However, few metho...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkt451
更新日期：2013-07-01 00:00:00
abstract：:Mutations in mammalian and Drosophila Hel308 and PolQ paralogues cause genome instability but their helicase functions are mysterious. By in vivo and in vitro analysis, we show that Hel308 from archaea (Hel308a) may act at stalled replication forks. Introducing hel308a into Escherichia coli dnaE strains that condition...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gki685
更新日期：2005-06-30 00:00:00
abstract：:U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Here we report the first example of naturally occurring U1-independent U2-type splicing in humans. The U1 components were not included in the pre-spliceosomal E complex formed on the human F1gamma (hF1gamma) intron 9 in vitro. Moreover, h...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkp050
更新日期：2009-04-01 00:00:00
abstract：:Using cyanoethyldiisopropylamino phosphoramidite chemistry, four oligonucleotides constituting a part of the sequence of the initiator t-RNA from B. subtilis were synthesized. For the protection of the exocyclic amino functions of bases, phenoxyacetyl group was used for adenine and guanine, and acetyl group was prefer...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.18.7381
更新日期：1989-09-25 00:00:00
abstract：:A microchip method has been developed for massive and parallel thermodynamic analyses of DNA duplexes. Fluorescently labeled oligonucleotides were hybridized with oligonucleotides immobilized in the 100 x 100 x 20 mum gel pads of the microchips. The equilibrium melting curves for all microchip duplexes were measured i...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.6.1515
更新日期：1998-03-15 00:00:00
abstract：:We describe a database of macromolecular motions meant to be of general use to the structural community. The database, which is accessible on the World Wide Web with an entry point at http://bioinfo.mbb.yale.edu/MolMovDB , attempts to systematize all instances of protein and nucleic acid movement for which there is at...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.18.4280
更新日期：1998-09-15 00:00:00
abstract：:Several different computational problems have been solved using DNA as a medium. However, the DNA computations that have so far been carried out have examined a relatively small number of possible sequence solutions in order to find correct sequence solutions. We have encoded a search algorithm in DNA that required th...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.22.5203
更新日期：1998-11-15 00:00:00
abstract：:The nucleotide sequence of the celD gene, encoding the previously crystallized endoglucanase D of Clostridium thermocellum, is reported. The enzyme shares a conserved, reiterated domain with the COOH-terminal end of endoglucanases A and B from the same organism. The overexpression in Escherichia coli of celD subcloned...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/14.21.8605
更新日期：1986-11-11 00:00:00
