Abstract:
:The investigation of RNA structure, dynamics and biological function often requires the site-specific incorporation of non-natural moieties. Here we describe the functionalization of RNA transcripts by aldehyde-hydrazine chemistry using a simple initiator nucleotide that carries an acetal-protected aldehyde function. This initiator nucleotide was efficiently incorporated into RNA, and the modified RNAs were quantitatively coupled to a peptide derivative displaying a hydrazine moiety at one end, a biotin tag at the other, and a trypsin-cleavable sequence in between. RNA conjugates could be easily isolated by affinity chromatography on streptavidin agarose and quantitatively cleaved off the support by trypsin treatment without detectable RNA degradation. The strategy described here may allow the incorporation of various new features into enzymatically synthesized RNA under mild conditions.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Pfander S,Fiammengo R,Kirin SI,Metzler-Nolte N,Jäschke Adoi
10.1093/nar/gkl1110subject
Has Abstractpub_date
2007-01-01 00:00:00pages
e25issue
4eissn
0305-1048issn
1362-4962pii
gkl1110journal_volume
35pub_type
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