Dun1, a Chk2-related kinase, is the central regulator of securin-separase dynamics during DNA damage signaling.

abstract：:A 15-mer DNA aptamer (named TBA) adopts a G-quadruplex structure that strongly inhibits fibrin-clot formation by binding to thrombin. We have performed thermodynamic analysis, binding affinity and biological activity studies of TBA variants modified by unlocked nucleic acid (UNA) monomers. UNA-U placed in position U3,...

journal_title：Nucleic acids research

authors： Pasternak A,Hernandez FJ,Rasmussen LM,Vester B,Wengel J

更新日期：2011-02-01 00:00:00

abstract：:We have examined the behaviour of the beta-interferon promoter in mouse F9 embryonal carcinoma cells. In undifferentiated cells, the beta-interferon promoter is not responsive to dsRNA or to Sendai virus. In cells stimulated to differentiate into parietal endoderm by treatment with retinoic acid, the beta-interferon p...

journal_title：Nucleic acids research

authors： Ellis MJ,Goodbourn S

更新日期：1994-10-25 00:00:00

abstract：:Recombinase proteins assembled into helical filaments on DNA are believed to be the catalytic core of homologous recombination. The assembly, disassembly and dynamic rearrangements of this structure must drive the DNA strand exchange reactions of homologous recombination. The sensitivity of eukaryotic recombinase acti...

journal_title：Nucleic acids research

authors： Ristic D,Modesti M,van der Heijden T,van Noort J,Dekker C,Kanaar R,Wyman C

更新日期：2005-06-08 00:00:00

abstract：:The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which...

journal_title：Nucleic acids research

authors： Fleck O,Michael H,Heim L

更新日期：1992-05-11 00:00:00

abstract：:The Ribosomal Database Project (RDP-II) provides the research community with aligned and annotated rRNA gene sequences, along with analysis services and a phylogenetically consistent taxonomic framework for these data. Updated monthly, these services are made available through the RDP-II website (http://rdp.cme.msu.ed...

journal_title：Nucleic acids research

authors： Cole JR,Chai B,Farris RJ,Wang Q,Kulam SA,McGarrell DM,Garrity GM,Tiedje JM

更新日期：2005-01-01 00:00:00

abstract：:It is informative to detect highly conserved positions in proteins and nucleic acid sequence/structure since they are often indicative of structural and/or functional importance. ConSurf (http://consurf.tau.ac.il) and ConSeq (http://conseq.tau.ac.il) are two well-established web servers for calculating the evolutionar...

journal_title：Nucleic acids research

authors： Ashkenazy H,Erez E,Martz E,Pupko T,Ben-Tal N

更新日期：2010-07-01 00:00:00

abstract：:DNA polymerases alpha and delta are essential enzymes believed to play critical roles in initiation and replication of chromosome DNA. In this study, we show that the genes for Schizosaccharomyces pombe (S.pombe) DNA polymerase alpha and delta (pol alpha+ and pol delta+) are essential for cell viability. Disruption of...

journal_title：Nucleic acids research

authors： Francesconi S,Park H,Wang TS

更新日期：1993-08-11 00:00:00

abstract：:This report documents the error rate in a commercially distributed subset of the IMAGE Consortium mouse cDNA clone collection. After isolation of plasmid DNA from 1189 bacterial stock cultures, only 62. 2% were uncontaminated and contained cDNA inserts that had significant sequence identity to published data for the o...

journal_title：Nucleic acids research

authors： Halgren RG,Fielden MR,Fong CJ,Zacharewski TR

更新日期：2001-01-15 00:00:00

abstract：:Analysis of haplotypes is an important tool in population genetics, familial heredity and gene mapping. Determination of haplotypes of multiple single nucleotide polymorphisms (SNPs) or other simple mutations is time consuming and expensive when analyzing large populations, and often requires the help of computational...

journal_title：Nucleic acids research

authors： Eitan Y,Kashi Y

更新日期：2002-06-15 00:00:00

abstract：:We have isolated the poly(A) polymerase (PAP) encoding gene pla1 [for poly(A) polymerase] from the fission yeast Schizosaccharomyces pombe. Protein sequence alignments with other poly(A) polymerases reveal that pla1 is more closely related to Saccharomyces cerevisiae PAP than to bovine PAP. The two yeast poly(A) polym...

journal_title：Nucleic acids research

authors： Ohnacker M,Minvielle-Sebastia L,Keller W

更新日期：1996-07-01 00:00:00

abstract：:Reactome (http://www.reactome.org) is a manually curated open-source open-data resource of human pathways and reactions. The current version 46 describes 7088 human proteins (34% of the predicted human proteome), participating in 6744 reactions based on data extracted from 15 107 research publications with PubMed link...

journal_title：Nucleic acids research

authors： Croft D,Mundo AF,Haw R,Milacic M,Weiser J,Wu G,Caudy M,Garapati P,Gillespie M,Kamdar MR,Jassal B,Jupe S,Matthews L,May B,Palatnik S,Rothfels K,Shamovsky V,Song H,Williams M,Birney E,Hermjakob H,Stein L,D'Eusta

更新日期：2014-01-01 00:00:00

abstract：:Xenopus laevis 5S rRNA isolated from 7S particles or transcribed in vitro is found to adopt two alternative conformations. These two conformers contain different structural elements within the major TF III A binding domain, which, when isolated as RNA fragments, still interact with the transcription factor. Chemical m...

journal_title：Nucleic acids research

authors： Pieler T,Guddat U,Oei SL,Erdmann VA

更新日期：1986-08-11 00:00:00

abstract：:microRNAs (miRNAs) are small endogenous RNA molecules that are implicated in many biological processes through post-transcriptional regulation of gene expression. The DIANA-microT Web server provides a user-friendly interface for comprehensive computational analysis of miRNA targets in human and mouse. The server has ...

journal_title：Nucleic acids research

authors： Maragkakis M,Vergoulis T,Alexiou P,Reczko M,Plomaritou K,Gousis M,Kourtis K,Koziris N,Dalamagas T,Hatzigeorgiou AG

更新日期：2011-07-01 00:00:00

abstract：:We have isolated from a single human liver cDNA library two clones which are highly homologous (78% over the coding region) to the major phenobarbital-inducible P450 from rat (P450IIB1). This is the first direct demonstration of the presence of the P450IIB gene subfamily in humans. This subfamily is much less extensiv...

journal_title：Nucleic acids research

authors： Miles JS,Spurr NK,Gough AC,Jowett T,McLaren AW,Brook JD,Wolf CR

更新日期：1988-07-11 00:00:00

abstract：:The mammalian transcription factor SRF and the yeast regulatory protein MCM1 contain DNA binding domains that are 70% identical; moreover, both proteins can bind the serum response element in the human c-fos promoter. Here we present an analysis of MCM1 sequence specificity by selection of sites from random sequence o...

journal_title：Nucleic acids research

authors： Wynne J,Treisman R

更新日期：1992-07-11 00:00:00

abstract：:Cryptic splice sites are used only when use of a natural splice site is disrupted by mutation. To determine the features that distinguish authentic from cryptic 5' splice sites (5'ss), we systematically analyzed a set of 76 cryptic 5'ss derived from 46 human genes. These cryptic 5'ss have a similar frequency distribut...

journal_title：Nucleic acids research

authors： Roca X,Sachidanandam R,Krainer AR

更新日期：2003-11-01 00:00:00

abstract：:We present the complete nucleotide sequence of the larval beta I-globin gene of Xenopus laevis including 240 nucleotides of the 5' flanking region and 594 nucleotides beyond the polyadenylation site. The site of transcription initiation was mapped by S1 nuclease, and the site of polyadenylation was determined by compa...

journal_title：Nucleic acids research

authors： Meyerhof W,Klinger-Mitropoulos S,Stalder J,Weber R,Knöchel W

更新日期：1984-10-25 00:00:00

abstract：:Redefinition of UAG, UAA and UGA to specify a standard amino acid occurs in response to recoding signals present in a minority of mRNAs. This 'read-through' is in competition with termination and is utilized for gene expression. One of the recoding signals known to stimulate read-through is a hexanucleotide sequence o...

journal_title：Nucleic acids research

authors： Harrell L,Melcher U,Atkins JF

更新日期：2002-05-01 00:00:00

abstract：:We have examined the formation of alternate strand triple-helices at the target sequence A11(TC)6.(GA)6T11 using the oligonucleotides T11(AG)6 and T11(TG)6, by DNase I footprinting. These third strands were designed so as to form parallel T.AT triplets together with antiparallel G.GC and A.AT or T.AT triplets. We find...

journal_title：Nucleic acids research

authors： Washbrook E,Fox KR

更新日期：1994-09-25 00:00:00

abstract：:Negative superhelical strain induces the poly(dG)-poly(dC) sequence to adopt two totally different types of triple-helices, either a dG.dG.dC triplex in the presence of Mg(+)+ at both neutral and acidic pHs or a protonated dC+.dG.dC triplex in the absence of Mg(+)+ ions at acidic pH (1). To examine whether there are s...

journal_title：Nucleic acids research

authors： Kohwi-Shigematsu T,Kohwi Y

更新日期：1991-08-11 00:00:00

abstract：:During protein synthesis, the nascent peptide chain traverses the peptide exit tunnel of the ribosome. We monitor the co-translational movement of the nascent peptide using a fluorescent probe attached to the N-terminus of the nascent chain. Due to fluorophore quenching, the time-dependent fluorescence signal emitted ...

journal_title：Nucleic acids research

authors： Haase N,Holtkamp W,Lipowsky R,Rodnina M,Rudorf S

更新日期：2018-12-14 00:00:00

abstract：:Recent studies have employed cross-species comparisons of transcription factor binding, reporting significant regulatory network 'rewiring' between species. Here, we address how a transcriptional repressor targets and regulates neural genes differentially between human and mouse embryonic stem cells (ESCs). We find th...

journal_title：Nucleic acids research

authors： Rockowitz S,Zheng D

更新日期：2015-07-13 00:00:00

abstract：:Characterization of the epigenetic status of individual cells remains a challenge. Current sequencing approaches have limited coverage, and it is difficult to assign an epigenetic status to the transcription state of individual gene alleles in the same cell. To address these limitations, a targeted microscopy-based ep...

journal_title：Nucleic acids research

authors： Kint S,Van Criekinge W,Vandekerckhove L,De Vos WH,Bomsztyk K,Krause DS,Denisenko O

更新日期：2021-01-28 00:00:00

abstract：:Transcription start and termination sites were mapped in the E. coli replication origin, oriC. Outward transcription from within oriC (promoters Pori-r and Pori-l) was found to start in vivo at position 178 for Pori-l and at positions 294 and 304 for Pori-r, respectively. These transcripts were terminated after 100-15...

journal_title：Nucleic acids research

authors： Schauzu MA,Kücherer C,Kölling R,Messer W,Lother H

更新日期：1987-03-25 00:00:00

abstract：:To determine whether methylation changes in specific DNA sequences of the albumin and AFP genes are implicated in the modulation of transcriptional activity during rat liver development and neoplasia we have analysed the methylation pattern of C-C-G-G sequences within these genes in DNA isolated from fetal and adult h...

journal_title：Nucleic acids research

authors： Vedel M,Gomez-Garcia M,Sala M,Sala-Trepat JM

更新日期：1983-07-11 00:00:00

abstract：:The 3'-untranslated region (UTR) of tobacco mosaic virus (TMV), which terminates in a tRNA-like structure, functionally substitutes for a poly(A) tail in both plant and animal cells. The addition of the TMV 3'-UTR to chimeric mRNA constructs increases their expression up to 100-fold, increasing both translational effi...

journal_title：Nucleic acids research

authors： Gallie DR,Feder JN,Schimke RT,Walbot V

更新日期：1991-09-25 00:00:00

abstract：:Horizontally acquired genes are typically regulated by ancestral regulators. This regulation enables expression of horizontally acquired genes to be coordinated with that of preexisting genes. Here, we report a singular example of the opposite regulation: a horizontally acquired gene that controls an ancestral regulat...

journal_title：Nucleic acids research

authors： Choi J,Groisman EA

更新日期：2020-11-04 00:00:00

abstract：:Holliday junction (HJ) resolving enzyme RecU is involved in DNA repair and recombination. We have determined the crystal structure of inactive mutant (D88N) of RecU from Bacillus subtilis in complex with a 12 base palindromic DNA fragment at a resolution of 3.2 Å. This structure shows the stalk region and the essentia...

journal_title：Nucleic acids research

authors： Khavnekar S,Dantu SC,Sedelnikova S,Ayora S,Rafferty J,Kale A

更新日期：2017-01-25 00:00:00

abstract：:The recent publication of the Caenorhabditis elegans cisRED database has provided an extensive catalog of upstream elements that are conserved between nematode genomes. We have performed a secondary analysis to determine which subsequences of the cisRED motifs are found in multiple locations throughout the C. elegans ...

journal_title：Nucleic acids research

authors： Sleumer MC,Mah AK,Baillie DL,Jones SJ

更新日期：2010-05-01 00:00:00

abstract：:The first step in DNA cleavage at V(D)J recombination signals by RAG1 and RAG2 is creation of a nick at the heptamer/coding flank border. Under proper conditions in vitro the second step, hairpin formation, requires two signals with spacers of 12 and 23 bp, a restriction referred to as the 12/23 rule. Under these cond...

journal_title：Nucleic acids research

authors： Eastman QM,Schatz DG

更新日期：1997-11-01 00:00:00