The primary structure of the larval beta 1-globin gene of Xenopus laevis and its flanking regions.

abstract：:In an extensive analysis, using a range of restriction endonucleases, HinfI and TaqI were found to differentiate satellites I, II and III & IV. Satellite I is resistant to digestion by TaqI, but is cleaved by HinfI to yield three major fragments of approximate size 770, 850 and 950bp, associated in a single length of ...

journal_title：Nucleic acids research

authors： Frommer M,Prosser J,Tkachuk D,Reisner AH,Vincent PC

更新日期：1982-01-22 00:00:00

abstract：:Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins and ribosomal RNA (rRNA), including the processing of the latter. Signalling through mammalian target of rapamycin complex 1 (mTORC1) activates all the...

journal_title：Nucleic acids research

authors： Liu R,Iadevaia V,Averous J,Taylor PM,Zhang Z,Proud CG

更新日期：2014-04-01 00:00:00

abstract：:Insertion of the insertion sequence Is2(I) directly before the galE gene of the galactose operon results in a Gal minus phenotype (1, 2). The Gal-constitutive allele galc200 (and its deletion derivative galc200 delta 31) arise from such a Gal minus mutant by the insertion of LS2(II) DNA within the LS2(I) sequence (3)....

journal_title：Nucleic acids research

authors： Hinton DM,Musso RE

更新日期：1982-08-25 00:00:00

abstract：:Microsatellites, such as (TG)n found at random throughout the genome, or as 3' extensions of Alu sequences are being increasingly used as genetic markers because of their pluriallelic character. The search for polymorphic microsatellites is time consuming, however, as it is necessary to sequence clones containing the ...

journal_title：Nucleic acids research

authors： Charlieu JP,Laurent AM,Carter DA,Bellis M,Roizès G

更新日期：1992-03-25 00:00:00

abstract：:Numerous RNA-binding proteins have modular structures, comprising one or several copies of a selective RNA-binding domain generally coupled to an auxiliary domain that binds RNA non-specifically. We have built and compared homology-based models of the cold-shock domain (CSD) of the Xenopus protein, FRGY2, and of the t...

journal_title：Nucleic acids research

authors： Manival X,Ghisolfi-Nieto L,Joseph G,Bouvet P,Erard M

更新日期：2001-06-01 00:00:00

abstract：:The Bovine Genome Database (BGD) (http://bovinegenome.org) has been the key community bovine genomics database for more than a decade. To accommodate the increasing amount and complexity of bovine genomics data, BGD continues to advance its practices in data acquisition, curation, integration and efficient data retrie...

journal_title：Nucleic acids research

authors： Shamimuzzaman M,Le Tourneau JJ,Unni DR,Diesh CM,Triant DA,Walsh AT,Tayal A,Conant GC,Hagen DE,Elsik CG

更新日期：2020-01-08 00:00:00

abstract：:The crystal structure of the RNA/DNA hybrid r(GAAGAGAAGC). d(GCTTCTCTTC) has been solved and refined at 2.5 A resolution. The refinement procedure converged at R = 0.181 for all reflections in the range 20.0-2.5 A. In the crystal, the RNA/DNA hybrid duplex has an A' conformation with all but one of the nucleotide suga...

journal_title：Nucleic acids research

authors： Conn GL,Brown T,Leonard GA

更新日期：1999-01-15 00:00:00

abstract：:RNA pseudoknots are functional structure elements with key roles in viral and cellular processes. Prediction of a pseudoknotted minimum free energy structure is an NP-complete problem. Practical algorithms for RNA structure prediction including restricted classes of pseudoknots suffer from high runtime and poor accura...

journal_title：Nucleic acids research

authors： Sperschneider J,Datta A

更新日期：2010-04-01 00:00:00

abstract：:We have examined the behaviour of the beta-interferon promoter in mouse F9 embryonal carcinoma cells. In undifferentiated cells, the beta-interferon promoter is not responsive to dsRNA or to Sendai virus. In cells stimulated to differentiate into parietal endoderm by treatment with retinoic acid, the beta-interferon p...

journal_title：Nucleic acids research

authors： Ellis MJ,Goodbourn S

更新日期：1994-10-25 00:00:00

abstract：:Restriction mapping demonstrated the presence of several distinct proviral forms of mouse mammary tumor virus in the genome of GR mice. One of these proviruses (GR-MTV-2) was highly amplified in GR 3A cells, a cell line derived from a GR mammary tumor. By the criterion of restriction mapping, the amplified GR-MTV-2 pr...

journal_title：Nucleic acids research

authors： Fanning TG,Puma JP,Cardiff RD

更新日期：1980-12-11 00:00:00

abstract：:Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imin...

journal_title：Nucleic acids research

authors： Kierzek R,He L,Turner DH

更新日期：1992-04-11 00:00:00

abstract：:Genomic DNA methylation contributes substantively to transcriptional regulations that underlie mammalian development and cellular differentiation. Much effort has been made to decipher the molecular mechanisms governing the establishment and maintenance of DNA methylation patterns. However, little is known about genom...

journal_title：Nucleic acids research

authors： Xie H,Wang M,de Andrade A,Bonaldo Mde F,Galat V,Arndt K,Rajaram V,Goldman S,Tomita T,Soares MB

更新日期：2011-05-01 00:00:00

abstract：:Inducible promoters are a central regulatory component in synthetic biology, metabolic engineering, and protein production for laboratory and commercial uses. Many of these applications utilize two or more exogenous promoters, imposing a currently unquantifiable metabolic burden on the living system. Here, we engineer...

journal_title：Nucleic acids research

authors： Davey JA,Wilson CJ

更新日期：2020-09-25 00:00:00

abstract：:The proteins in a cell often assemble into complexes to carry out their functions and play an essential role of biological processes. The PCFamily server identifies template-based homologous protein complexes [called protein complex family (PCF)] and infers functional modules of the query proteins. This server first f...

journal_title：Nucleic acids research

authors： Lo YS,Lin CY,Yang JM

更新日期：2010-07-01 00:00:00

abstract：:The effect of several rifamycin derivatives on poly(A) synthesis in vitro was tested using purified rat liver mitochondrial poly(A) polymerase assayed with an exogenous primer. When used at a concentration of 300 mug/ml, derivatives AF/013, PR/19, AF/AETP, M/88 and AF/ABDP completely inhibited activity corresponding t...

journal_title：Nucleic acids research

authors： Jacob ST,Rose KM

更新日期：1974-11-01 00:00:00

abstract：:This article presents W-IQ-TREE, an intuitive and user-friendly web interface and server for IQ-TREE, an efficient phylogenetic software for maximum likelihood analysis. W-IQ-TREE supports multiple sequence types (DNA, protein, codon, binary and morphology) in common alignment formats and a wide range of evolutionary ...

journal_title：Nucleic acids research

authors： Trifinopoulos J,Nguyen LT,von Haeseler A,Minh BQ

更新日期：2016-07-08 00:00:00

abstract：:The structure in solution of an RNA fragment (218 nucleotides long) containing part of E. coli 16S rRNA domain 2 has been studied using the intrinsic photoaffinity probe 4-thiouridine (s4U). In vitro transcription with T7 polymerase, in the presence of s4U triphosphate yielded complete RNA molecules. An affinity elect...

journal_title：Nucleic acids research

authors： Dubreuil YL,Expert-Bezançon A,Favre A

更新日期：1991-07-11 00:00:00

abstract：:The history and mechanism of molecular evolution in DNA have been greatly elucidated by contributions from genetics, probability theory and bioinformatics--indeed, mathematical developments such as Kimura's neutral theory, Kingman's coalescent theory and efficient software such as BLAST, ClustalW, Phylip, etc., provid...

journal_title：Nucleic acids research

authors： Waldispühl J,Devadas S,Berger B,Clote P

更新日期：2009-07-01 00:00:00

abstract：:DNA polymerase III (DNA pol III) efficiently replicates the Escherichia coli genome, but it cannot bypass DNA damage. Instead, translesion synthesis (TLS) DNA polymerases are employed to replicate past damaged DNA; however, the exchange of replicative for TLS polymerases is not understood. The umuD gene products, whic...

journal_title：Nucleic acids research

authors： Silva MC,Nevin P,Ronayne EA,Beuning PJ

更新日期：2012-07-01 00:00:00

abstract：:Four phage clones which hybridize with U1 small nuclear RNA were obtained from a rat gene library. Two clones contain a presumed pseudogene. A third clone includes two gene candidates that are co-linear with the rat U1-RNA, 3.6kb apart and in the opposite orientation. The two genes are surrounded by identical sequence...

journal_title：Nucleic acids research

authors： Watanabe-Nagasu N,Itoh Y,Tani T,Okano K,Koga N,Okada N,Ohshima Y

更新日期：1983-03-25 00:00:00

abstract：:Nucleosomes, a basic structural unit of eukaryotic chromatin, play a significant role in regulating gene expression. We have developed a web tool based on DNA sequences known from empirical and theoretical studies to influence DNA bending and flexibility, and to exclude nucleosomes. NXSensor (available at http://www.s...

journal_title：Nucleic acids research

authors： Luykx P,Bajić IV,Khuri S

更新日期：2006-07-01 00:00:00

abstract：:We have investigated the distribution of mitochondrial DNA polymorphisms in a rare maternally transmitted genetic trait that causes hypersensitivity to aminoglycoside antibiotics, in the hope that a characterization of its molecular basis might provide a molecular and cellular understanding of aminoglycoside-induced d...

journal_title：Nucleic acids research

authors： Hutchin T,Haworth I,Higashi K,Fischel-Ghodsian N,Stoneking M,Saha N,Arnos C,Cortopassi G

更新日期：1993-09-11 00:00:00

abstract：:We demonstrate compaction of DNA with nanoscale biomimetic constructs which are robust synthetic analogs of globular proteins. These constructs are approximately 15 nm in diameter, shell crosslinked knedel-like (SCKs) nanoparticles, which are prepared by covalent stabilization of amphiphilic di-block co-polymer micell...

journal_title：Nucleic acids research

authors： Thurmond KB 2nd,Remsen EE,Kowalewski T,Wooley KL

更新日期：1999-07-15 00:00:00

abstract：:A physical mapping strategy has been developed to verify and accelerate the assembly and gap closure phase of a microbial genome shotgun-sequencing project. The protocol was worked out during the ongoing Pseudomonas putida KT2440 genome project. A macro-restriction map was constructed by linking probe hybridisation of...

journal_title：Nucleic acids research

authors： Weinel C,Tümmler B,Hilbert H,Nelson KE,Kiewitz C

更新日期：2001-11-15 00:00:00

abstract：:Tetrahymena thermophila mitochondrial DNA is a linear molecule with two tRNAs, large subunit beta (LSU beta) rRNA (21S rRNA) and LSU alpha rRNA (5.8S-like RNA) encoded near each terminus. The DNA sequence of approximately 550 bp of this region was determined in six species of Tetrahymena. In three species the LSU beta...

journal_title：Nucleic acids research

authors： Morin GB,Cech TR

更新日期：1988-01-11 00:00:00

abstract：:The secondary structure of V4, the largest variable area of eukaryotic small subunit ribosomal RNA, was re-examined by comparative analysis of 3253 nucleotide sequences distributed over the animal, plant and fungal kingdoms and a diverse set of protist taxa. An extensive search for compensating base pair substitutions...

journal_title：Nucleic acids research

authors： Wuyts J,De Rijk P,Van de Peer Y,Pison G,Rousseeuw P,De Wachter R

更新日期：2000-12-01 00:00:00

abstract：:We have isolated and sequenced the complete murine I-E alpha immune response gene of the H-2db haplotype. The I-E alpha d gene consists of 5300 basepairs and is organized into five or possibly six exons that correspond to different domains of the alpha chain. The amino acid sequence deduced from the I-E alpha gene sho...

journal_title：Nucleic acids research

authors： Hyldig-Nielsen JJ,Schenning L,Hammerling U,Widmark E,Heldin E,Lind P,Servenius B,Lund T,Flavell R,Lee JS,Trowsdale J,Schreier PH,Zablitzky F,Larhammar D,Peterson PA,Rask L

更新日期：1983-08-11 00:00:00

abstract：:FlyBase is a database of genetic and molecular data concerning Drosophila. FlyBase is maintained as a relational database (in Sybase). The scope of FlyBase includes: genes, alleles (and phenotypes), aberrations, pointers to sequence data, clones, stock lists, Drosophila workers and bibliographic references. FlyBase is...

journal_title：Nucleic acids research

authors： Flybase Consortium.

更新日期：1996-01-01 00:00:00

abstract：:Nematode mitochondria expresses two types of extremely truncated tRNAs that are specifically recognized by two distinct elongation factor Tu (EF-Tu) species named EF-Tu1 and EF-Tu2. This is unlike the canonical EF-Tu molecule that participates in the standard protein biosynthesis systems, which basically recognizes al...

journal_title：Nucleic acids research

authors： Suematsu T,Sato A,Sakurai M,Watanabe K,Ohtsuki T

更新日期：2005-08-19 00:00:00

abstract：:The alternatively spliced RNA species of tumor suppressor gene p53, containing an additional 96 bases derived from intron 10, is present at approximately 25 to 30% the level of regularly spliced p53 RNA in both normal epidermal and carcinoma cells. The presence of this alternatively spliced RNA in 10T1/2 fibroblast ce...

journal_title：Nucleic acids research

authors： Han KA,Kulesz-Martin MF

更新日期：1992-04-25 00:00:00