3' Alu PCR: a simple and rapid method to isolate human polymorphic markers.

abstract：:Recent studies indicate that controlling the nuclear decondensation and intra-nuclear localization of plasmid DNA (pDNA) would result in an increased transfection efficiency. In the present study, we established a technology for imaging the nuclear condensation/decondensation status of pDNA in nuclear subdomains using...

journal_title：Nucleic acids research

authors： Shaheen SM,Akita H,Yamashita A,Katoono R,Yui N,Biju V,Ishikawa M,Harashima H

更新日期：2011-04-01 00:00:00

abstract：:Abasic sites (AP-sites) are frequent DNA lesions, arising by spontaneous base hydrolysis or as intermediates of base excision repair (BER). The hemiacetal at the anomeric centre renders them chemically reactive, which presents a challenge to biochemical and structural investigation. Chemically more stable AP-site anal...

journal_title：Nucleic acids research

authors： Schuermann D,Scheidegger SP,Weber AR,Bjørås M,Leumann CJ,Schär P

更新日期：2016-03-18 00:00:00

abstract：:Calf thymus nucleosomes exhibit two different and independent hydrodynamic responses to diminishing salt concentration. One change is gradual over the range 40 to 0.2 mM Na+ and is accompanied by decreases in contact-site cross-linking efficiency. The other change is abrupt, being centered between 1 and 2 mM Na+. We f...

journal_title：Nucleic acids research

authors： Burch JB,Martinson HG

更新日期：1980-11-11 00:00:00

abstract：:Two genomic DNAs with a size of approximately 2.8 kb, isolated from the liver of Bungarus multicinctus (Taiwan banded krait), encode the precursors of the long neurotoxins, alpha-Bgt(A31) and alpha-Bgt(V31), respectively. Both genes share virtually identical overall organization with three exons separated by two intro...

journal_title：Nucleic acids research

authors： Chang L,Lin S,Huang H,Hsiao M

更新日期：1999-10-15 00:00:00

abstract：:The MPI Bioinformatics Toolkit (http://toolkit.tuebingen.mpg.de) is an open, interactive web service for comprehensive and collaborative protein bioinformatic analysis. It offers a wide array of interconnected, state-of-the-art bioinformatics tools to experts and non-experts alike, developed both externally (e.g. BLAS...

journal_title：Nucleic acids research

authors： Alva V,Nam SZ,Söding J,Lupas AN

更新日期：2016-07-08 00:00:00

abstract：:APOBEC3G (A3G), a host protein that inhibits HIV-1 reverse transcription and replication in the absence of Vif, displays cytidine deaminase and single-stranded (ss) nucleic acid binding activities. HIV-1 nucleocapsid protein (NC) also binds nucleic acids and has a unique property, nucleic acid chaperone activity, whic...

journal_title：Nucleic acids research

authors： Iwatani Y,Chan DS,Wang F,Stewart-Maynard K,Sugiura W,Gronenborn AM,Rouzina I,Williams MC,Musier-Forsyth K,Levin JG

更新日期：2007-01-01 00:00:00

abstract：:The small subunit ribosomal RNA gene (16S rRNA) has been successfully used to catalogue and study the diversity of prokaryotic species and communities but it offers limited resolution at the species and finer levels, and cannot represent the whole-genome diversity and fluidity. To overcome these limitations, we introd...

journal_title：Nucleic acids research

authors： Rodriguez-R LM,Gunturu S,Harvey WT,Rosselló-Mora R,Tiedje JM,Cole JR,Konstantinidis KT

更新日期：2018-07-02 00:00:00

abstract：:RNA molecules of > 20 nucleotides have been the focus of numerous recent NMR structural studies. Several investigators have used the UNCG family of hairpins to ensure proper folding. We show that th UUCG hairpin has a minimum requirement of a two base-pair stem. Hairpins with a CG loop closing base pair and an initial...

journal_title：Nucleic acids research

authors： Molinaro M,Tinoco I Jr

更新日期：1995-08-11 00:00:00

abstract：:Transgenic mice suitable for the in vivo assay of suspected mutagens at the chromosome level have been constructed by stable integration of a lambda phage shuttle vector. The shuttle vector, which contains a beta-galactosidase (beta-gal) target gene, can be rescued from genomic DNA with in vitro packaging extracts. Mu...

journal_title：Nucleic acids research

authors： Kohler SW,Provost GS,Kretz PL,Dycaico MJ,Sorge JA,Short JM

更新日期：1990-05-25 00:00:00

abstract：:The EcoRV DNA-(adenine-N(6))-methyltransferase (M.EcoRV) specifically modifies the first adenine residue within GATATC sequences. During catalysis, the enzyme flips its target base out of the DNA helix and binds it into a target base binding pocket which is formed in part by Lys16 and Tyr196. A cytosine residue is acc...

journal_title：Nucleic acids research

authors： Roth M,Jeltsch A

更新日期：2001-08-01 00:00:00

abstract：:Dinucleoside diphosphates of the general type pGpN have been prepared enzymatically using ribonuclease N1. Alkylated uridines or cytidines, which are products of carcinogens acting on nucleic acids, were tested in dinucleoside diphosphates for their ability to stimulate the binding of Ala- or Val-tRNA to ribosomes. O2...

journal_title：Nucleic acids research

authors： Singer B,Pergolizzi RG,Grunberger D

更新日期：1979-04-01 00:00:00

abstract：:We have previously characterized an extracellular nuclease from Pseudomonas BAL 31 which, in addition to other activities, displays a double-strand exonuclease activity which progressively shortens both strands of linear duplex DNA molecules from both termini. This degradation is accomplished without the introduction ...

journal_title：Nucleic acids research

authors： Legerski RJ,Hodnett JL,Gray HB Jr

更新日期：1978-05-01 00:00:00

abstract：:Spirochetes of the genus Borrelia include the tick-transmitted causative agents of Lyme disease and relapsing fever. They possess unusual genomes composed mainly of linear replicons terminated by closed DNA hairpin telomeres. Hairpin telomeres present an uninterrupted DNA chain to the replication machinery overcoming ...

journal_title：Nucleic acids research

authors： Mir T,Huang SH,Kobryn K

更新日期：2013-12-01 00:00:00

abstract：:We have analyzed the ATPase activity exhibited by the UvrABC DNA repair complex. The UvrA protein is an ATPase whose lack of DNA dependence may be related to the ATP induced monomer-dimer transitions. ATP induced dimerization may be responsible for the enhanced DNA binding activity observed in the presence of ATP. Alt...

journal_title：Nucleic acids research

authors： Oh EY,Claassen L,Thiagalingam S,Mazur S,Grossman L

更新日期：1989-06-12 00:00:00

abstract：:Based on parameters governing promoter activity and using regulatory elements of the lac, ara and tet operon transcription control sequences were composed which permit the regulation in Escherichia coli of several gene activities independently and quantitatively. The novel promoter PLtetO-1 allows the regulation of ge...

journal_title：Nucleic acids research

authors： Lutz R,Bujard H

更新日期：1997-03-15 00:00:00

abstract：:Proteomic and RNomic approaches have identified many components of different ribonucleoprotein particles (RNPs), yet still little is known about the organization and protein proximities within these heterogeneous and highly dynamic complexes. Here we describe a targeted cross-linking approach, which combines cross-lin...

journal_title：Nucleic acids research

authors： Trahan C,Oeffinger M

更新日期：2016-02-18 00:00:00

abstract：:The Homeodomain Resource is a comprehensive collection of sequence, structure and genomic information on the homeodomain protein family. Available through the Resource are both full-length and domain-only sequence data, as well as X-ray and NMR structural data for proteins and protein-DNA complexes. Also available is ...

journal_title：Nucleic acids research

authors： Banerjee-Basu S,Ferlanti ES,Ryan JF,Baxevanis AD

更新日期：1999-01-01 00:00:00

abstract：:Bacteria occupy a diverse set of environmental niches with differing oxygen availability. Anaerobic environments such as mammalian digestive tracts and industrial reactors harbor an abundance of both obligate and facultative anaerobes, many of which play significant roles in human health and biomanufacturing. Studying...

journal_title：Nucleic acids research

authors： Wang XC,Wilson SC,Hammond MC

更新日期：2016-09-30 00:00:00

abstract：:To screen multiple loci in small purified samples of diploid and aneuploid cells a PCR-based technique of whole genome amplification was adapted to the study of somatic lesions. DNA samples from different numbers of flow-sorted diploid and aneuploid cells from biopsies were amplified with a degenerate 15mer primer. Al...

journal_title：Nucleic acids research

authors： Barrett MT,Reid BJ,Joslyn G

更新日期：1995-09-11 00:00:00

abstract：:Bacterial retrons consist of a reverse transcriptase (RT) and a contiguous non-coding RNA (ncRNA) gene. One third of annotated retrons carry additional open reading frames (ORFs), the contribution and significance of which in retron biology remains to be determined. In this study we developed a computational pipeline ...

journal_title：Nucleic acids research

authors： Mestre MR,González-Delgado A,Gutiérrez-Rus LI,Martínez-Abarca F,Toro N

更新日期：2020-12-16 00:00:00

abstract：:DnaD and DnaB are essential DNA-replication-initiation proteins in low-G+C content Gram-positive bacteria. Here we use sensitive Hidden Markov Model-based techniques to show that the DnaB and DnaD proteins share a common structure that is evident across all their structural domains, termed DDBH1 and DDBH2 (DnaD DnaB H...

journal_title：Nucleic acids research

authors： Marston FY,Grainger WH,Smits WK,Hopcroft NH,Green M,Hounslow AM,Grossman AD,Craven CJ,Soultanas P

更新日期：2010-11-01 00:00:00

abstract：:Transcriptional interference between adjacent genes has been demonstrated in a variety of biological systems. To study this process in RNA polymerase II (pol II) transcribed genes we have analysed the effect of transcription on tandem HIV-1 promoters integrated into the genome of HeLa cells. We show that transcription...

journal_title：Nucleic acids research

authors： Greger IH,Demarchi F,Giacca M,Proudfoot NJ

更新日期：1998-03-01 00:00:00

abstract：:Mouse globin messenger ribonucleoprotein (mRNP) has been isolated from reticulocyte polysomes by affinity chromatography to oligo(T)-cellulose, using a procedure modified from that of Lindberg and Sundquist. The messenger RNA and protein moieties fo the mRNP are indistinguishable from those isolated by less rapid tech...

journal_title：Nucleic acids research

authors： Burns AT,Williamson R

更新日期：1975-12-01 00:00:00

abstract：:The current release of ProTherm, Thermodynamic Database for Proteins and Mutants, contains more than 10 000 numerical data (300% of the first version) of several thermodynamic parameters, experimental methods and conditions, reversibility of folding, details about the surrounding residues in space for all mutants, str...

journal_title：Nucleic acids research

authors： Gromiha MM,Uedaira H,An J,Selvaraj S,Prabakaran P,Sarai A

更新日期：2002-01-01 00:00:00

abstract：:In the present study, the relationship between short interfering RNA (siRNA) sequence and RNA interference (RNAi) effect was extensively analyzed using 62 targets of four exogenous and two endogenous genes and three mammalian and Drosophila cells. We present the rules that may govern siRNA sequence preference and in a...

journal_title：Nucleic acids research

authors： Ui-Tei K,Naito Y,Takahashi F,Haraguchi T,Ohki-Hamazaki H,Juni A,Ueda R,Saigo K

更新日期：2004-02-09 00:00:00

abstract：:In this study we describe a novel method to investigate the RNA-RNA interactions between a small RNA and its target that we termed 'RNA walk'. The method is based on UV-induced AMT cross-linking in vivo followed by affinity selection of the hybrid molecules and mapping the intermolecular adducts by RT-PCR or real-time...

journal_title：Nucleic acids research

authors： Lustig Y,Wachtel C,Safro M,Liu L,Michaeli S

更新日期：2010-01-01 00:00:00

abstract：:A study of the activity of deoxyribonucleotide-substituted analogs of the hammerhead domain of RNA catalysis has led to the design of a 14mer oligomer composed entirely of deoxyribonucleotides that promotes the cleavage of an RNA substrate. Characterization of this reaction with sequence variants and mixed DNA/RNA oli...

journal_title：Nucleic acids research

authors： Chartrand P,Harvey SC,Ferbeyre G,Usman N,Cedergren R

更新日期：1995-10-25 00:00:00

abstract：:The last few years have witnessed significant changes in Pfam (https://pfam.xfam.org). The number of families has grown substantially to a total of 17,929 in release 32.0. New additions have been coupled with efforts to improve existing families, including refinement of domain boundaries, their classification into Pfa...

journal_title：Nucleic acids research

authors： El-Gebali S,Mistry J,Bateman A,Eddy SR,Luciani A,Potter SC,Qureshi M,Richardson LJ,Salazar GA,Smart A,Sonnhammer ELL,Hirsh L,Paladin L,Piovesan D,Tosatto SCE,Finn RD

更新日期：2019-01-08 00:00:00

abstract：:The nucleotide sequence of the faeD gene of Escherichia coli and the amino acid sequence of its product is presented. The faeD product is an outer membrane protein required for transport of K88ab fimbrial subunits across the outer membrane. The protein is synthesized as a precursor containing a signal peptide, and the...

journal_title：Nucleic acids research

authors： Mooi FR,Claassen I,Bakker D,Kuipers H,de Graaf FK

更新日期：1986-03-25 00:00:00

abstract：:The avian retrovirus pp32 DNA endonuclease prefers to nick supercoiled DNA containing long terminal repeat (LTR) circle junction sequences at one or the other of two sites, each which mapped two nucleotides back from the circle junction. The sequence at the sites of nicking was (sequence: see text) where increases ind...

journal_title：Nucleic acids research

authors： Grandgenett DP,Vora AC

更新日期：1985-09-11 00:00:00