Abstract:
:The first step in DNA cleavage at V(D)J recombination signals by RAG1 and RAG2 is creation of a nick at the heptamer/coding flank border. Under proper conditions in vitro the second step, hairpin formation, requires two signals with spacers of 12 and 23 bp, a restriction referred to as the 12/23 rule. Under these conditions hairpin formation occurs at the two signals at or near the same time. In contrast, we find that under the same conditions nicking occurs at isolated signals and hence is not subject to the 12/23 rule. With two signals the nicking events are not concerted and the signal with a 12 bp spacer is usually nicked first. However, the extent and rate of nicking at a given signal are diminished by mutations of the other signal. The appearance of DNA nicked at both signals is stimulated by more than an order of magnitude by the ability of the signals to synapse, indicating that synapsis accelerates nicking and often precedes it. These observations allow formulation of a more complete model of catalysis of DNA cleavage and how the 12/23 rule is enforced.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Eastman QM,Schatz DGdoi
10.1093/nar/25.21.4370subject
Has Abstractpub_date
1997-11-01 00:00:00pages
4370-8issue
21eissn
0305-1048issn
1362-4962pii
gka677journal_volume
25pub_type
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2003-09-15 00:00:00
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pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:1990-04-25 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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pub_type: 杂志文章
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更新日期:1984-12-21 00:00:00
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更新日期:2002-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-12-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-07-02 00:00:00
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journal_title:Nucleic acids research
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更新日期:2020-07-27 00:00:00
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journal_title:Nucleic acids research
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更新日期:2009-03-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkn057
更新日期:2008-06-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2016-06-02 00:00:00
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journal_title:Nucleic acids research
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journal_title:Nucleic acids research
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更新日期:1988-01-11 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/13.1.225
更新日期:1985-01-11 00:00:00
abstract::SMILE (small heterodimer partner interacting leucine zipper protein) has been identified as a coregulator in ER signaling. In this study, we have examined the effects of SMILE on other NRs (nuclear receptors). SMILE inhibits GR, CAR and HNF4 alpha-mediated transactivation. Knockdown of SMILE gene expression increases ...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkp333
更新日期:2009-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2004-01-02 00:00:00
abstract::PRED(BALB/c) is a computational system that predicts peptides binding to the major histocompatibility complex-2 (H2(d)) of the BALB/c mouse, an important laboratory model organism. The predictions include the complete set of H2(d) class I (H2-K(d), H2-L(d) and H2-D(d)) and class II (I-E(d) and I-A(d)) molecules. The p...
journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2005-07-01 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
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更新日期:2018-01-25 00:00:00
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journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkr988
更新日期:2012-01-01 00:00:00
abstract::Initiation of reverse transcription is a complex and regulated process in all retroviruses. Several base pairing interactions have been proposed to occur between the HIV-1 RNA genome and the specific tRNA(lys3) primer. The tRNA primer can form up to 21 bp with the primer binding site (PBS), and an additional 8 bp inte...
journal_title:Nucleic acids research
pub_type: 杂志文章
doi:10.1093/nar/gkg714
更新日期:2003-09-01 00:00:00