Abstract:
:The molecular structure of the DNA double helix has been known for 60 years, but we remain surprisingly ignorant of the balance of forces that determine its mechanical properties. The DNA double helix is among the stiffest of all biopolymers, but neither theory nor experiment has provided a coherent understanding of the relative roles of attractive base stacking forces and repulsive electrostatic forces creating this stiffness. To gain insight, we have created a family of double-helical DNA-like polymers where one of the four normal bases is replaced with various cationic, anionic or neutral analogs. We apply DNA ligase-catalyzed cyclization kinetics experiments to measure the bending and twisting flexibilities of these polymers under low salt conditions. Interestingly, we show that these modifications alter DNA bending stiffness by only 20%, but have much stronger (5-fold) effects on twist flexibility. We suggest that rather than modifying DNA stiffness through a mechanism easily interpretable as electrostatic, the more dominant effect of neutral and charged base modifications is their ability to drive transitions to helical conformations different from canonical B-form DNA.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
Peters JP,Yelgaonkar SP,Srivatsan SG,Tor Y,James Maher L 3rddoi
10.1093/nar/gkt808subject
Has Abstractpub_date
2013-12-01 00:00:00pages
10593-604issue
22eissn
0305-1048issn
1362-4962pii
gkt808journal_volume
41pub_type
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