Unexpected A-form formation of 4'-thioDNA in solution, revealed by NMR, and the implications as to the mechanism of nuclease resistance.

abstract：:The beta-globin gene of a patient with mRNA-deficient beta o-thalassemia has been sequenced. We find a single nucleotide deletion in amino acid codon 44 that produces a UGA terminator at codon 60. We have previously shown that the beta-globin mRNA of this patient is correctly spliced and polyadenylated, but rapidly tu...

journal_title：Nucleic acids research

authors： Kinniburgh AJ,Maquat LE,Schedl T,Rachmilewitz E,Ross J

更新日期：1982-09-25 00:00:00

abstract：:The strand displacement activity of DNA polymerase δ is strongly stimulated by its interaction with proliferating cell nuclear antigen (PCNA). However, inactivation of the 3'-5' exonuclease activity is sufficient to allow the polymerase to carry out strand displacement even in the absence of PCNA. We have examined in ...

journal_title：Nucleic acids research

authors： Koc KN,Stodola JL,Burgers PM,Galletto R

更新日期：2015-04-30 00:00:00

abstract：:A putative role for mammalian polynucleotide kinases that possess both 5'-phosphotransferase and 3'-phosphatase activity is the restoration of DNA strand breaks with 5'-hydroxyl termini or 3'-phosphate termini, or both, to a form that supports the subsequent action of DNA repair polymerases and DNA ligases, i.e. 5'-ph...

journal_title：Nucleic acids research

authors： Karimi-Busheri F,Lee J,Tomkinson AE,Weinfeld M

更新日期：1998-10-01 00:00:00

abstract：:Mitochondrial DNA is a sensitive target of chemical carcinogens (Backer and Weinstein (1980) Science 209, 297-299), suggesting that mutations of the mitochondrial genome occur in tumor cells. We examined this point by comparing mitochondrial DNA sequences in four rat tumors with those of normal rat liver. Some novel m...

journal_title：Nucleic acids research

authors： Taira M,Yoshida E,Kobayashi M,Yaginuma K,Koike K

更新日期：1983-03-25 00:00:00

abstract：:A C-->U mutation (rdn5) in the conserved sarcin/ricin domain of yeast 25S rRNA has been shown to cause translational suppression and paromomycin resistance. It also separates the killing from the misreading effect of this antibiotic. We confirm these findings and provide in vitro evidence that rdn5 causes a 3-fold inc...

journal_title：Nucleic acids research

authors： Panopoulos P,Dresios J,Synetos D

更新日期：2004-10-11 00:00:00

abstract：:The Deciphering the Mechanisms of Developmental Disorders (DMDD) consortium is a research programme set up to identify genes in the mouse, which if mutated (or knocked-out) result in embryonic lethality when homozygous, and initiate the study of why disruption of their function has such profound effects on embryo deve...

journal_title：Nucleic acids research

authors： Wilson R,McGuire C,Mohun T,DMDD Project.

更新日期：2016-01-04 00:00:00

abstract：:RNA-based therapeutics, i.e. the utilization of synthetic RNA molecules to alter cellular functions, have the potential to address targets which are currently out of scope for traditional drug design pipelines. This potential however hinges on the ability to selectively deliver and internalize therapeutic RNAs into ce...

journal_title：Nucleic acids research

authors： Wang Y,Hoinka J,Liang Y,Adamus T,Swiderski P,Przytycka TM

更新日期：2018-09-19 00:00:00

abstract：:The laboratory mouse is the premier animal model for studying human biology because all life stages can be accessed experimentally, a completely sequenced reference genome is publicly available and there exists a myriad of genomic tools for comparative and experimental research. In the current era of genome scale, dat...

journal_title：Nucleic acids research

authors： Bult CJ,Eppig JT,Blake JA,Kadin JA,Richardson JE,Mouse Genome Database Group.

更新日期：2013-01-01 00:00:00

abstract：:Generally, the presence of a consensus sequence in the promoter of a gene is taken as indication for regulation by the transcription factor that binds to this sequence. In light of the recent developments in genome research, we were interested to what extent this supposition is valid. We examined the relationship betw...

journal_title：Nucleic acids research

authors： Hamoen LW,Smits WK,de Jong A,Holsappel S,Kuipers OP

更新日期：2002-12-15 00:00:00

abstract：:Bacillus stearothermophilus large ribosomal subunits were reconstituted in the presence of 5S rRNAs from different origins and tested for their biological activities. The results obtained have shown that eubacterial and archaebacterial 5S rRNAs can easily substitute for B. stearothermophilus 5S rRNA in the reconstitut...

journal_title：Nucleic acids research

authors： Hartmann RK,Vogel DW,Walker RT,Erdmann VA

更新日期：1988-04-25 00:00:00

abstract：:DNA predominantly contains Watson-Crick (WC) base pairs, but a non-negligible fraction of base pairs are in the Hoogsteen (HG) hydrogen bonding motif at any time. In HG, the purine is rotated ∼180° relative to the WC motif. The transitions between WC and HG may play a role in recognition and replication, but are diffi...

journal_title：Nucleic acids research

authors： Vreede J,Pérez de Alba Ortíz A,Bolhuis PG,Swenson DWH

更新日期：2019-12-02 00:00:00

abstract：:Posttranslational modifications (PTMs) of histones represent a crucial regulatory mechanism of nucleosome and chromatin dynamics in various of DNA-based cellular processes, such as replication, transcription and DNA damage repair. Lysine succinylation (Ksucc) is a newly identified histone PTM, but its regulation and f...

journal_title：Nucleic acids research

authors： Jing Y,Ding D,Tian G,Kwan KCJ,Liu Z,Ishibashi T,Li XD

更新日期：2020-09-25 00:00:00

abstract：:The anticancer drug cisplatin reacts with DNA leading to the formation of interstrand and intrastrand cross-links that are the critical cytotoxic lesions. In contrast to cells bearing mutations in other components of the nucleotide excision repair apparatus (XPB, XPD, XPG and CSB), cells defective for the ERCC1-XPF st...

journal_title：Nucleic acids research

authors： De Silva IU,McHugh PJ,Clingen PH,Hartley JA

更新日期：2002-09-01 00:00:00

abstract：:As a trial practical application, we have applied optimized microfabricated electrophoresis devices, combined with enzymatic mutation detection methods, to the determination of single nucleotide polymorphism (SNP) sites in the p53 suppressor gene. Using clinical samples, we have achieved robust assays with quality fac...

journal_title：Nucleic acids research

authors： Schmalzing D,Belenky A,Novotny MA,Koutny L,Salas-Solano O,El-Difrawy S,Adourian A,Matsudaira P,Ehrlich D

更新日期：2000-05-01 00:00:00

abstract：:DNA lengths in the structural repeat units of Chinese hamster ovary (CHO) and chicken erythrocyte chromatin were compared by analyzing the sizes of DNA fragments produced after treatment of nuclei with staphylococcal nuclease. The repeat length of CHO chromatin (173 +- 4 BP) is about 20 base pairs (BP) smaller than th...

journal_title：Nucleic acids research

authors： Rill RL,Nelson DA,Oosterhof DK,Hozier JC

更新日期：1977-04-01 00:00:00

abstract：:For the isolation of single stranded plasmid DNA, various E. coli and E. coli-Streptomyces shuttle plasmids were equipped with the phage f1 replication origin. The transformation of some representative Streptomyces species with plasmid vectors occurred irrespective of whether single or double stranded DNA was used. In...

journal_title：Nucleic acids research

authors： Hillemann D,Pühler A,Wohlleben W

更新日期：1991-02-25 00:00:00

abstract：:The opening of DNA double strands is extremely relevant to several biological functions, such as replication and transcription or binding of specific proteins. Such opening phenomenon is particularly sensitive to the aqueous solvent conditions in which the DNA molecule is dispersed, as it can be observed by considerin...

journal_title：Nucleic acids research

authors： Tempestini A,Cassina V,Brogioli D,Ziano R,Erba S,Giovannoni R,Cerrito MG,Salerno D,Mantegazza F

更新日期：2013-02-01 00:00:00

abstract：:pS194 is a naturally occurring Staphylococcus aureus plasmid encoding streptomycin resistance. The plasmid has a copy number of about 25 per cell, and belongs to the inc5 incompatibility group. The nucleotide sequence of pS194 has been determined and consists of 4397 base pairs including four open reading frames poten...

journal_title：Nucleic acids research

authors： Projan SJ,Moghazeh S,Novick RP

更新日期：1988-03-25 00:00:00

abstract：:The nucleotide sequence of a 2.14 kb fragment of Bacillus subtilis DNA containing the citG gene encoding fumarase was determined using the dideoxy chain termination method. The citG coding region of 1392 base pairs (464 codons) was identified, and the deduced Mr (50425) is in good agreement with that of the protein id...

journal_title：Nucleic acids research

authors： Miles JS,Guest JR

更新日期：1985-01-11 00:00:00

abstract：:Epigenetic regulation of gene expression is tightly controlled by the dynamic modification of histones by chemical groups, the diversity of which has largely expanded over the past decade with the discovery of lysine acylations, catalyzed from acyl-coenzymes A. We investigated the dynamics of lysine acetylation and cr...

journal_title：Nucleic acids research

authors： Crespo M,Damont A,Blanco M,Lastrucci E,Kennani SE,Ialy-Radio C,Khattabi LE,Terrier S,Louwagie M,Kieffer-Jaquinod S,Hesse AM,Bruley C,Chantalat S,Govin J,Fenaille F,Battail C,Cocquet J,Pflieger D

更新日期：2020-05-07 00:00:00

abstract：:Multiple pathways counteract DNA replication stress to prevent genomic instability and tumorigenesis. The recently identified human SDE2 is a genome surveillance protein regulated by PCNA, a DNA clamp and processivity factor at replication forks. Here, we show that SDE2 cleavage after its ubiquitin-like domain generat...

journal_title：Nucleic acids research

authors： Rageul J,Park JJ,Jo U,Weinheimer AS,Vu TTM,Kim H

更新日期：2019-05-07 00:00:00

abstract：:We previously constructed a multiribozyme expression vector by combining cis- and trans-acting ribozymes and we showed that several ribozymes, each directed against a different target in the HIV genome and acting independently in a 'shotgun' manner, markedly increased the efficiency of cleavage of HIV RNA in vitro [Oh...

journal_title：Nucleic acids research

authors： Yuyama N,Ohkawa J,Koguma T,Shirai M,Taira K

更新日期：1994-11-25 00:00:00

abstract：:Purified 15 S globin mRNA-protein (mRNP) complexes obtained by EDTA dissociation of duck reticulocytes polyribosomes were digested with the calcium dependant Staphylococcus aureus nuclease (EC 3. 1. 4. 7.). 25% of the globin mRNA sequences were resistant to extensive nuclease digestion as determined by TCA precipitati...

journal_title：Nucleic acids research

authors： Goldenberg S,Vincent A,Scherrer K

更新日期：1979-06-25 00:00:00

abstract：:Four hundred twenty-eight high-resolution DNA-protein complexes were chosen for a bioinformatics study. Although 164 crystal structures (38% of those searched) contained no interactions, 574 discrete π-contacts between the aromatic amino acids and the DNA nucleobases or deoxyribose were identified using strict criteri...

journal_title：Nucleic acids research

authors： Wilson KA,Kellie JL,Wetmore SD

更新日期：2014-06-01 00:00:00

abstract：:Pds5 is required for sister chromatid cohesion, and somewhat paradoxically, to remove cohesin from chromosomes. We found that Pds5 plays a critical role during DNA replication that is distinct from its previously known functions. Loss of Pds5 hinders replication fork progression in unperturbed human and mouse cells. I...

journal_title：Nucleic acids research

authors： Carvajal-Maldonado D,Byrum AK,Jackson J,Wessel S,Lemaçon D,Guitton-Sert L,Quinet A,Tirman S,Graziano S,Masson JY,Cortez D,Gonzalo S,Mosammaparast N,Vindigni A

更新日期：2019-02-20 00:00:00

abstract：:Signal transducer and activator of transcription 6 (STAT6) is a regulator of transcription for interleukin-4 (IL-4)-induced genes. The ability of STAT6 to activate transcription depends on functional interaction with other transcription factors and coactivators. We have characterized the mechanism of STAT6-mediated tr...

journal_title：Nucleic acids research

authors： Välineva T,Yang J,Silvennoinen O

更新日期：2006-01-01 00:00:00

abstract：:Three new proteins which selectively bind to UV-damaged DNA were identified and purified to near homogeneity from UV-irradiated Drosophila melanogaster embryos through several column chromatographies. These proteins, tentatively designated as D-DDB P1, P2 and P3, can be identified as different complex bands in a gel s...

journal_title：Nucleic acids research

authors： Kai M,Takahashi T,Todo T,Sakaguchi K

更新日期：1995-07-25 00:00:00

abstract：:We have used a polymerase chain reaction (PCR) procedure to analyse low abundance complementary sense RNAs of Digitaria streak virus (DSV) from infected leaves of Digitaria setigera. This study has confirmed that both spliced and unspliced RNAs are synthesised by the same transcription unit. The position of the intron...

journal_title：Nucleic acids research

authors： Mullineaux PM,Guerineau F,Accotto GP

更新日期：1990-12-25 00:00:00

abstract：:Two CTAG-recognizing restriction and modification (R/M) systems, designated MthZI and MthFI, were identified in the thermophilic archaeon Methanobacterium thermoformicicum strains Z-245 and FTF, respectively. Further analysis revealed that the methyltransferase (MTase) genes are plasmid-located in both strains. The pl...

journal_title：Nucleic acids research

authors： Nölling J,de Vos WM

更新日期：1992-10-11 00:00:00

abstract：:The high-throughput sequencing of nuclease-protected mRNA fragments bound to ribosomes, a technique known as ribosome profiling, quantifies the relative frequencies with which different regions of transcripts are translated. This technique has revealed novel translation initiation sites with unprecedented scope and ha...

journal_title：Nucleic acids research

authors： Martens AT,Taylor J,Hilser VJ

更新日期：2015-04-20 00:00:00