Abstract：
:The anticancer drug cisplatin reacts with DNA leading to the formation of interstrand and intrastrand cross-links that are the critical cytotoxic lesions. In contrast to cells bearing mutations in other components of the nucleotide excision repair apparatus (XPB, XPD, XPG and CSB), cells defective for the ERCC1-XPF structure-specific nuclease are highly sensitive to cisplatin. To determine if the extreme sensitivity of XPF and ERCC1 cells to cisplatin results from specific defects in the repair of either intrastrand or interstrand cross-links we measured the elimination of both lesions in a range of nucleotide excision repair Chinese hamster mutant cell lines, including XPF- and ERCC1-defective cells. Compared to the parental, repair-proficient cell line all the mutants tested were defective in the elimination of both classes of adduct despite their very different levels of increased sensitivity. Consequently, there is no clear relationship between initial incisions at interstrand cross-links or removal of intrastrand adducts and cellular sensitivity. These results demonstrate that the high cisplatin sensitivity of ERCC1 and XPF cells likely results from a defect other than in excision repair. In contrast to other conventional DNA cross-linking agents, we found that the repair of cisplatin adducts does not involve the formation of DNA double-strand breaks. Surprisingly, XRCC2 and XRCC3 cells are defective in the uncoupling step of cisplatin interstrand cross-link repair, suggesting that homologous recombination might be initiated prior to excision of this type of cross-link.
journal_name
Nucleic Acids Resjournal_title
Nucleic acids researchauthors
De Silva IU,McHugh PJ,Clingen PH,Hartley JAdoi
10.1093/nar/gkf479keywords:
subject
Has Abstractpub_date
2002-09-01 00:00:00pages
3848-56issue
17eissn
0305-1048issn
1362-4962journal_volume
30pub_type
杂志文章abstract：:Current knowledge of parameters affecting RNA stability is very restricted in plants. Here we investigated factors which might contribute to the stability of a particular plant messenger RNA. To this end, insertion and deletion mutants were made in two different exons and an intron of the transcribed region of a well ...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/18.10.2917
更新日期：1990-05-25 00:00:00
abstract：:We have identified and sequenced two members of a chicken middle repetitive DNA sequence family. By reassociation kinetics, members of this family (termed CRl) are estimated to be present in 1500-7000 copies per chicken haploid genome. The first family member sequenced (CRlUla) is located approximately 2 kb upstream f...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/9.20.5383
更新日期：1981-10-24 00:00:00
abstract：:Given that greater than 90% of the human genome is expressed, it is logical to assume that post-transcriptional regulatory mechanisms must be the primary means of controlling the flow of information from mRNA to protein. This report describes a robust approach that includes in silico, in vitro and in cellulo experimen...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkq557
更新日期：2010-11-01 00:00:00
abstract：:No analogous nucleoside triphosphate was found which acts as well as ATP in binding to and supporting catalysis of leucyl-tRNA synthetase from Escherichia coli MRE 600. However, there are numerous nucleotides which are able to replace ATP, but with lower efficiency. The 6-amino group of the adenine ring and the 2'-hyd...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/3.8.2067
更新日期：1976-08-01 00:00:00
abstract：:The DNA damage checkpoint halts cell cycle progression in G2 in response to genotoxic insults. Central to the execution of cell cycle arrest is the checkpoint-induced stabilization of securin-separase complex (yeast Pds1-Esp1). The checkpoint kinases Chk1 and Chk2 (yeast Chk1 and Rad53) are thought to critically contr...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa355
更新日期：2020-06-19 00:00:00
abstract：:We report thermodynamic values for binding of the guanosine nucleophile to the ribozyme derived from the Anabaena group I intron, and find that they are similar to those measured previously for the structurally distinct Tetrahymena ribozyme. The free energy of binding guanosine 5'-monophosphate (pG) at 30 degrees C is...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.19.3722
更新日期：1996-10-01 00:00:00
abstract：:This paper examines theoretically the effects that restraints on the tertiary structure of a superhelical DNA domain exert on the energetics of linking and the onset of conformational transitions. The most important tertiary constraint arises from the nucleosomal winding of genomic DNA in vivo. Conformational transiti...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/15.23.9985
更新日期：1987-12-10 00:00:00
abstract：:Using a combination of bioinformatic and molecular biology approaches a Drosophila melanogaster protein, DmTTF, has been identified, which exhibits sequence and structural similarity with two mitochondrial transcription termination factors, mTERF (human) and mtDBP (sea urchin). Import/processing assays indicate that D...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg272
更新日期：2003-03-15 00:00:00
abstract：:A downstream G-rich sequence (GRS), GGGGGAGGUGUGGG, has been previously shown to influence the efficiency of 3' end processing of the SV40 late polyadenylation signal. We have now defined several important parameters for GRS-mediated polyadenylation. The ability of the GRS to influence 3' end processing efficiency was...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/23.9.1625
更新日期：1995-05-11 00:00:00
abstract：:The contacts of phosphate groups in mRNAs with ribosomes were studied. Two mRNAs were used: one mRNA contained in the middle two defined codons to construct the pre- and the post-translocational states, the other was a sequence around the initiation site of the natural cro-mRNA. Phosphorothioate nucleotides were rando...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/24.12.2228
更新日期：1996-06-15 00:00:00
abstract：:We present version 2 of the SPINE system for structural proteomics. SPINE is available over the web at http://nesg.org. It serves as the central hub for the Northeast Structural Genomics Consortium, allowing collaborative structural proteomics to be carried out in a distributed fashion. The core of SPINE is a laborato...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkg397
更新日期：2003-06-01 00:00:00
abstract：:Cdc13 is an essential protein from Saccharomyces cerevisiae that caps telomeres by protecting the C-rich telomeric DNA strand from degradation and facilitates telomeric DNA replication by telomerase. In vitro, Cdc13 binds TG-rich single-stranded telomeric DNA with high affinity and specificity. A previously identified...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkf554
更新日期：2002-10-01 00:00:00
abstract：:O-GLYCBASE is a revised database of information on glycoproteins and their O-linked glycosylation sites. Entries are compiled and revised from the literature, and from the sequence databases. Entries include information about species, sequence, glycosylation sites and glycan type and is fully cross-referenced. Compare...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/26.1.387
更新日期：1998-01-01 00:00:00
abstract：:The NCBI Taxonomy database (http://www.ncbi.nlm.nih.gov/taxonomy) is the standard nomenclature and classification repository for the International Nucleotide Sequence Database Collaboration (INSDC), comprising the GenBank, ENA (EMBL) and DDBJ databases. It includes organism names and taxonomic lineages for each of the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr1178
更新日期：2012-01-01 00:00:00
abstract：:Topoisomerase II is an essential enzyme that is required for virtually every process that requires movement of DNA within the nucleus or the opening of the double helix. This enzyme helps to regulate DNA under- and overwinding and removes knots and tangles from the genetic material. In order to carry out its critical ...
journal_title：Nucleic acids research
pub_type： 杂志文章,评审
doi：10.1093/nar/gkn937
更新日期：2009-02-01 00:00:00
abstract：:H/ACA small nucleolar RNAs (snoRNAs) guide pseudouridylation as part of a small nucleolar ribonucleoprotein complex (snoRNP). Disruption of H/ACA snoRNA levels in stem cells impairs pluripotency, yet it remains unclear how H/ACA snoRNAs contribute to differentiation. To determine if H/ACA snoRNA levels are dynamic dur...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkaa612
更新日期：2020-09-04 00:00:00
abstract：:We created a single cell sorting system to screen for enzyme activity in Escherichia coli producing 3,4 dihydroxy benzoate (34DHB). To do so, we engineered a transcription factor regulon controlling the expression of green fluorescent protein (GFP) for induction by 34DHB. An autoregulated transcription factor, pcaU, w...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gku444
更新日期：2014-07-01 00:00:00
abstract：:Endonucleases that generate double-strand breaks in DNA often possess two identical subunits related by rotational symmetry, arranged so that the active sites from each subunit act on opposite DNA strands. In contrast to many endonucleases, Type IIP restriction enzyme BcnI, which recognizes the pseudopalindromic seque...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr588
更新日期：2011-11-01 00:00:00
abstract：:Human adenovirus mutants that carry a large deletion in early region 4 (E4) are severely defective in the synthesis of viral late proteins. Plasmids that carry intact E4 sequences can complement the late protein synthetic defect of such mutants when introduced into infected cells by transfection, presumably due to the...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/17.8.3037
更新日期：1989-04-25 00:00:00
abstract：:eggNOG is a public database of orthology relationships, gene evolutionary histories and functional annotations. Here, we present version 5.0, featuring a major update of the underlying genome sets, which have been expanded to 4445 representative bacteria and 168 archaea derived from 25 038 genomes, as well as 477 euka...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky1085
更新日期：2019-01-08 00:00:00
abstract：:The molecular mechanisms that underlie the neurological manifestations of patients with inherited diseases of vitamin B12 (cobalamin) metabolism remain to date obscure. We observed transcriptomic changes of genes involved in RNA metabolism and endoplasmic reticulum stress in a neuronal cell model with impaired cobalam...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gky634
更新日期：2018-09-06 00:00:00
abstract：:While antibiotic selection has been routinely used for the selection of genetically modified cells, administration of cytotoxic drugs often leads to deleterious effects not only to inert cells but also to transfected or transduced ones. In this study, we propose an Antigen-MEdiated Genetically modified cell Amplificat...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gng032
更新日期：2003-04-01 00:00:00
abstract：:The linking number of DNA in minichromosomes increases by 2 turns during SV40 assembly. Changes in temperature also influence the average linking number of the total intracellular forms of SV40 DNA. When the isolated minichromosomes assembled in vivo are incubated with topoisomerase I at 33 degrees C in vitro, the lin...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/15.9.3703
更新日期：1987-05-11 00:00:00
abstract：:Guanine-quadruplexes (G4) consist of non-canonical four-stranded helical arrangements of guanine-rich nucleic acid sequences. The bulky and thermodynamically stable features of G4 structures have been shown in many respects to affect normal nucleic acid metabolism. In vivo conversion of G4 structures to single-strande...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkr630
更新日期：2011-11-01 00:00:00
abstract：:We have analyzed multiple recombinant DNA clones containing ribosomal RNA repeat units of the silkmoth, Bombyx mori. In combination with genomic DNA blots, analysis of these clones indicated that the rDNA repeat of B. mori is 10.8 kilobase pair in length and tandemly repeated in the genome, as reported by Manning et a...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/12.11.4703
更新日期：1984-06-11 00:00:00
abstract：:Replication protein A (RPA) plays a critical role in all eukaryotic DNA processing involving single-stranded DNA (ssDNA). Contrary to the notion that RPA provides solely inert protection to transiently formed ssDNA, the RPA-ssDNA complex acts as a dynamic DNA processing unit. Here, we studied the diffusion of RPA alon...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkz1202
更新日期：2020-02-28 00:00:00
abstract：:Ribosomal DNA (rDNA) from Xenopus laevis contains several heterogeneities in all three transcribed spacers, as revealed by analysis of cloned and uncloned amplified rDNA from oocytes and cloned chromosomal rDNA from erythrocytes. Heterogeneities include single base changes and length variants of one to several nucleot...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/11.3.629
更新日期：1983-02-11 00:00:00
abstract：:Unwanted side effects of drugs are a burden on patients and a severe impediment in the development of new drugs. At the same time, adverse drug reactions (ADRs) recorded during clinical trials are an important source of human phenotypic data. It is therefore essential to combine data on drugs, targets and side effects...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkv1075
更新日期：2016-01-04 00:00:00
abstract：:Phosphorylation of the cellular single-stranded DNA-binding protein, replication protein A (RPA), occurs during normal mitotic cell cycle progression and also in response to genotoxic stress. In budding yeast, these reactions require the ATM homolog Mec1, a central regulator of the DNA replication and DNA damage check...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/29.23.4808
更新日期：2001-12-01 00:00:00
abstract：:Recent evidence indicates a link between Parkinson's Disease (PD) and the expression of a-synuclein (SNCA) isoforms with different 3' untranslated regions (3'UTRs). Yet, the post-transcriptional mechanisms regulating SNCA expression are unknown. Using a large-scale in vitro /in silico screening we identified RNA-bindi...
journal_title：Nucleic acids research
pub_type： 杂志文章
doi：10.1093/nar/gkx1048
更新日期：2017-12-15 00:00:00
